Restriction map for
pFN21AA0791
(Linear) (Six-base) MAPSORT of: KIAA0791.seq Check: 7322 from: 1 to: 3996
KIAA0791.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 904 3996
Size: 904 3092
AccI GT'mk_AC
Cuts at: 0 335 1701 2960 3801 3996
Size: 335 1366 1259 841 195
Fragments arranged by size:
1366 1259 841 335 195
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 2140 2236 2296 2692 3139 3271 3996
Size: 569 1571 96 60 396 447 132 725
Fragments arranged by size:
1571 725 569 447 396 132 96 60
AflIII A'CryG_T
Cuts at: 0 1301 3804 3996
Size: 1301 2503 192
Fragments arranged by size:
2503 1301 192
AlwNI CAG_nnn'CTG
Cuts at: 0 382 2055 2109 2196 3996
Size: 382 1673 54 87 1800
Fragments arranged by size:
1800 1673 382 87 54
ApoI r'AATT_y
Cuts at: 0 497 870 1318 1978 3146 3238 3394 3581
Size: 497 373 448 660 1168 92 156 187
Cuts at: 3581 3957 3996
Size: 376 39
Fragments arranged by size:
1168 660 497 448 376 373 187 156
92 39
AseI AT'TA_AT
Cuts at: 0 2332 3996
Size: 2332 1664
AvaI C'yCGr_G
Cuts at: 0 1532 3179 3996
Size: 1532 1647 817
Fragments arranged by size:
1647 1532 817
BamHI G'GATC_C
Cuts at: 0 3278 3753 3996
Size: 3278 475 243
Fragments arranged by size:
3278 475 243
BanII G_rGCy'C
Cuts at: 0 601 1056 1203 2924 3532 3914 3928 3996
Size: 601 455 147 1721 608 382 14 68
Fragments arranged by size:
1721 608 601 455 382 147 68 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 1460 2699 3996
Size: 1460 1239 1297
Fragments arranged by size:
1460 1297 1239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2158 2192 3996
Size: 2158 34 1804
Fragments arranged by size:
2158 1804 34
BclI T'GATC_A
Cuts at: 0 3033 3732 3996
Size: 3033 699 264
Fragments arranged by size:
3033 699 264
BfrBI ATG'CAT
Cuts at: 0 2744 3135 3546 3996
Size: 2744 391 411 450
Fragments arranged by size:
2744 450 411 391
BglI GCCn_nnn'nGGC
Cuts at: 0 544 3996
Size: 544 3452
BglII A'GATC_T
Cuts at: 0 2295 3996
Size: 2295 1701
BmrI ACTGGGnnnn_n'
Cuts at: 0 1992 2795 3669 3962 3996
Size: 1992 803 874 293 34
Fragments arranged by size:
1992 874 803 293 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 187 3996
Size: 103 84 3809
Fragments arranged by size:
3809 103 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1709 2449 2810 3022 3898 3996
Size: 404 1305 740 361 212 876 98
Fragments arranged by size:
1305 876 740 404 361 212 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2633 3544 3996
Size: 2633 911 452
Fragments arranged by size:
2633 911 452
BsaI GGTCTCn'nnnn_
Cuts at: 0 212 1625 3996
Size: 212 1413 2371
Fragments arranged by size:
2371 1413 212
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1978 2008 3996
Size: 1978 30 1988
Fragments arranged by size:
1988 1978 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 230 1318 1424 2780 3522 3996
Size: 230 1088 106 1356 742 474
Fragments arranged by size:
1356 1088 742 474 230 106
BseYI C'CCAG_C
Cuts at: 0 1746 2090 3835 3996
Size: 1746 344 1745 161
Fragments arranged by size:
1746 1745 344 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2065 3493 3769 3996
Size: 2065 1428 276 227
Fragments arranged by size:
2065 1428 276 227
BsiEI CG_ry'CG
Cuts at: 0 828 3996
Size: 828 3168
BsiHKAI G_wGCw'C
Cuts at: 0 601 1056 1737 2577 2924 3532 3914 3928
Size: 601 455 681 840 347 608 382 14
Cuts at: 3928 3996
Size: 68
Fragments arranged by size:
840 681 608 601 455 382 347 68
14
BsmI GAATG_Cn'
Cuts at: 0 419 789 3548 3996
Size: 419 370 2759 448
Fragments arranged by size:
2759 448 419 370
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1697 3996
Size: 1697 2299
Bsp1286I G_dGCh'C
Cuts at: 0 601 1056 1203 1737 2577 2924 3532 3914
Size: 601 455 147 534 840 347 608 382
Cuts at: 3914 3928 3996
Size: 14 68
Fragments arranged by size:
840 608 601 534 455 382 347 147
68 14
BspHI T'CATG_A
Cuts at: 0 2124 3246 3996
Size: 2124 1122 750
Fragments arranged by size:
2124 1122 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 904 3633 3996
Size: 904 2729 363
Fragments arranged by size:
2729 904 363
BsrBI CCG'CTC
Cuts at: 0 2788 3996
Size: 2788 1208
BsrDI GCAATG_nn'
Cuts at: 0 809 3173 3706 3996
Size: 809 2364 533 290
Fragments arranged by size:
2364 809 533 290
BsrFI r'CCGG_y
Cuts at: 0 3448 3996
Size: 3448 548
BsrGI T'GTAC_A
Cuts at: 0 1290 3333 3624 3996
Size: 1290 2043 291 372
Fragments arranged by size:
2043 1290 372 291
BssSI C'ACGA_G
Cuts at: 0 3107 3996
Size: 3107 889
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2335 3996
Size: 2335 1661
BstBI TT'CG_AA
Cuts at: 0 2178 3996
Size: 2178 1818
BstEII G'GTnAC_C
Cuts at: 0 3898 3996
Size: 3898 98
BstYI r'GATC_y
Cuts at: 0 429 2295 2643 3278 3753 3996
Size: 429 1866 348 635 475 243
Fragments arranged by size:
1866 635 475 429 348 243
BstZ17I GTA'TAC
Cuts at: 0 1702 3802 3996
Size: 1702 2100 194
Fragments arranged by size:
2100 1702 194
BtgI C'CryG_G
Cuts at: 0 1025 3996
Size: 1025 2971
BtsI GCAGTG_nn'
Cuts at: 0 130 866 1493 1907 3206 3781 3996
Size: 130 736 627 414 1299 575 215
Fragments arranged by size:
1299 736 627 575 414 215 130
ClaI AT'CG_AT
Cuts at: 0 824 3996
Size: 824 3172
DraI TTT'AAA
Cuts at: 0 840 1250 1440 2292 3411 3848 3963 3996
Size: 840 410 190 852 1119 437 115 33
Fragments arranged by size:
1119 852 840 437 410 190 115 33
EaeI y'GGCC_r
Cuts at: 0 3023 3996
Size: 3023 973
EarI CTCTTCn'nnn_
Cuts at: 0 72 449 1415 1589 3974 3996
Size: 72 377 966 174 2385 22
Fragments arranged by size:
2385 966 377 174 72 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1577 3996
Size: 1577 2419
EcoICRI GAG'CTC
Cuts at: 0 599 1054 2922 3530 3912 3926 3996
Size: 599 455 1868 608 382 14 70
Fragments arranged by size:
1868 608 599 455 382 70 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 569 1709 2140 2236 2296 2449 2692
Size: 404 165 1140 431 96 60 153 243
Cuts at: 2692 2810 3022 3139 3271 3898 3996
Size: 118 212 117 132 627 98
Fragments arranged by size:
1140 627 431 404 243 212 165 153
132 118 117 98 96 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 23 3996
Size: 23 3973
EcoO109I rG'GnC_Cy
Cuts at: 0 28 3996
Size: 28 3968
EcoRV GAT'ATC
Cuts at: 0 2406 3492 3996
Size: 2406 1086 504
Fragments arranged by size:
2406 1086 504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2575 2980 3996
Size: 2575 405 1016
Fragments arranged by size:
2575 1016 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 810 842 1978 2010 3901 3933
Size: 388 32 390 32 1136 32 1891 32
Cuts at: 3933 3996
Size: 63
Fragments arranged by size:
1891 1136 390 388 63 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1125 1721 2961 3996
Size: 1125 596 1240 1035
Fragments arranged by size:
1240 1125 1035 596
HindIII A'AGCT_T
Cuts at: 0 1863 3382 3996
Size: 1863 1519 614
Fragments arranged by size:
1863 1519 614
MfeI C'AATT_G
Cuts at: 0 176 671 845 1913 3996
Size: 176 495 174 1068 2083
Fragments arranged by size:
2083 1068 495 176 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1600 2682 3306 3996
Size: 1600 1082 624 690
Fragments arranged by size:
1600 1082 690 624
MscI TGG'CCA
Cuts at: 0 3025 3996
Size: 3025 971
MslI CAynn'nnrTG
Cuts at: 0 914 1765 3996
Size: 914 851 2231
Fragments arranged by size:
2231 914 851
MspA1I CmG'CkG
Cuts at: 0 1177 2274 2374 3354 3996
Size: 1177 1097 100 980 642
Fragments arranged by size:
1177 1097 980 642 100
NdeI CA'TA_TG
Cuts at: 0 119 3996
Size: 119 3877
NsiI A_TGCA'T
Cuts at: 0 2746 3137 3548 3996
Size: 2746 391 411 448
Fragments arranged by size:
2746 448 411 391
NspI r_CATG'y
Cuts at: 0 913 1305 3135 3550 3808 3996
Size: 913 392 1830 415 258 188
Fragments arranged by size:
1830 913 415 392 258 188
PciI A'CATG_T
Cuts at: 0 1301 3804 3996
Size: 1301 2503 192
Fragments arranged by size:
2503 1301 192
PfoI T'CCnGG_A
Cuts at: 0 1002 3996
Size: 1002 2994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1677 1709 3996
Size: 1677 32 2287
Fragments arranged by size:
2287 1677 32
PpuMI rG'GwC_Cy
Cuts at: 0 28 3996
Size: 28 3968
PsiI TTA'TAA
Cuts at: 0 2942 3996
Size: 2942 1054
PspXI vC'TCGA_Gb
Cuts at: 0 3179 3996
Size: 3179 817
PstI C_TGCA'G
Cuts at: 0 2114 2634 3996
Size: 2114 520 1362
Fragments arranged by size:
2114 1362 520
PvuI CG_AT'CG
Cuts at: 0 828 3996
Size: 828 3168
PvuII CAG'CTG
Cuts at: 0 1177 2274 3354 3996
Size: 1177 1097 1080 642
Fragments arranged by size:
1177 1097 1080 642
SacI G_AGCT'C
Cuts at: 0 601 1056 2924 3532 3914 3928 3996
Size: 601 455 1868 608 382 14 68
Fragments arranged by size:
1868 608 601 455 382 68 14
SalI G'TCGA_C
Cuts at: 0 2959 3996
Size: 2959 1037
SexAI A'CCwGG_T
Cuts at: 0 30 2817 3996
Size: 30 2787 1179
Fragments arranged by size:
2787 1179 30
SfcI C'TryA_G
Cuts at: 0 332 1370 2110 2630 2980 3996
Size: 332 1038 740 520 350 1016
Fragments arranged by size:
1038 1016 740 520 350 332
SmlI C'TyrA_G
Cuts at: 0 2648 3179 3559 3996
Size: 2648 531 380 437
Fragments arranged by size:
2648 531 437 380
SphI G_CATG'C
Cuts at: 0 3550 3996
Size: 3550 446
SspI AAT'ATT
Cuts at: 0 690 1883 3996
Size: 690 1193 2113
Fragments arranged by size:
2113 1193 690
StuI AGG'CCT
Cuts at: 0 2713 3996
Size: 2713 1283
StyI C'CwwG_G
Cuts at: 0 2912 3573 3996
Size: 2912 661 423
Fragments arranged by size:
2912 661 423
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 3996
Size: 130 3866
TatI w'GTAC_w
Cuts at: 0 1044 1290 1796 3111 3170 3333 3624 3649
Size: 1044 246 506 1315 59 163 291 25
Cuts at: 3649 3939 3996
Size: 290 57
Fragments arranged by size:
1315 1044 506 291 290 246 163 59
57 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 593 625 2999 3031 3996
Size: 593 32 2374 32 965
Fragments arranged by size:
2374 965 593 32 32
XbaI T'CTAG_A
Cuts at: 0 682 1983 3996
Size: 682 1301 2013
Fragments arranged by size:
2013 1301 682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1174 3996
Size: 1174 2822
XhoI C'TCGA_G
Cuts at: 0 3179 3996
Size: 3179 817
XmnI GAAnn'nnTTC
Cuts at: 0 3242 3989 3996
Size: 3242 747 7
Fragments arranged by size:
3242 747 7
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AseI AvaI
BamHI BanII BbsI BcgI BclI BfrBI BglI BglII
BmrI BplI BpmI BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtsI ClaI DraI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NdeI
NsiI NspI PciI PfoI PpiI PpuMI PsiI PspXI
PstI PvuI PvuII SacI SalI SexAI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BciVI BlpI Bme1580I BmgBI BmtI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsiWI BspEI BssHII
BstXI Bsu36I BtgZI CspCI DraIII DrdI EagI EcoRI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PshAI PspOMI PsrI RsrII
SacII SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XmaI
ZraI