(Circular) (Six-base) MAPSORT of: pFN21AA0756.seq Check: 2090 from: 1 to: 8212 pFN21AA0756 8212 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2380 5017 2380 Size: 2637 5575 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6022 278 Size: 53 83 186 936 4486 2468 Fragments arranged by size: 4486 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4371 4539 5555 1937 Size: 2434 168 1016 4594 Fragments arranged by size: 4594 2434 1016 168 Acc65I G'GTAC_C Cuts at: 5533 5533 Size: 8212 AclI AA'CG_TT Cuts at: 1563 6339 6712 1563 Size: 4776 373 3063 Fragments arranged by size: 4776 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3731 4503 4508 4628 5265 6275 7659 7927 Size: 2099 772 5 120 637 1010 1384 268 Cuts at: 7927 1632 Size: 1917 Fragments arranged by size: 2099 1917 1384 1010 772 637 268 120 5 AfeI AGC'GCT Cuts at: 4148 4148 Size: 8212 AflII C'TTAA_G Cuts at: 820 1017 2716 820 Size: 197 1699 6316 Fragments arranged by size: 6316 1699 197 AflIII A'CryG_T Cuts at: 1203 2548 2733 3807 3809 7111 8088 1203 Size: 1345 185 1074 2 3302 977 1327 Fragments arranged by size: 3302 1345 1327 1074 977 185 2 AgeI A'CCGG_T Cuts at: 7043 7043 Size: 8212 AhdI GACnn_n'nnGTC Cuts at: 4990 6941 4990 Size: 1951 6261 AleI CACnn'nnGTG Cuts at: 1166 3650 1166 Size: 2484 5728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3668 1184 Size: 2484 5728 AlwNI CAG_nnn'CTG Cuts at: 5955 7527 5955 Size: 1572 6640 ApaI G_GGCC'C Cuts at: 4424 4424 Size: 8212 ApaLI G'TGCA_C Cuts at: 6269 7425 6269 Size: 1156 7056 ApoI r'AATT_y Cuts at: 1493 2082 2354 5521 5747 5899 8008 1493 Size: 589 272 3167 226 152 2109 1697 Fragments arranged by size: 3167 2109 1697 589 272 226 152 AseI AT'TA_AT Cuts at: 160 1032 6766 160 Size: 872 5734 1606 Fragments arranged by size: 5734 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8212 AvaI C'yCGr_G Cuts at: 1870 1943 2250 2745 3620 3885 3941 4172 4308 Size: 73 307 495 875 265 56 231 136 Cuts at: 4308 5537 1870 Size: 1229 4545 Fragments arranged by size: 4545 1229 875 495 307 265 231 136 73 56 AvrII C'CTAG_G Cuts at: 4796 4796 Size: 8212 BamHI G'GATC_C Cuts at: 2379 3936 5542 5909 2379 Size: 1557 1606 367 4682 Fragments arranged by size: 4682 1606 1557 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2789 3175 3644 5330 5533 Size: 325 217 627 1002 386 469 1686 203 Cuts at: 5533 6988 618 Size: 1455 1842 Fragments arranged by size: 1842 1686 1455 1002 627 469 386 325 217 203 BanII G_rGCy'C Cuts at: 1391 2532 4343 4424 5036 5531 1391 Size: 1141 1811 81 612 495 4072 Fragments arranged by size: 4072 1811 1141 612 495 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 3762 3999 5253 5409 928 Size: 971 1071 792 237 1254 156 3731 Fragments arranged by size: 3731 1254 1071 971 792 237 156 BbvCI CC'TCA_GC Cuts at: 5012 5012 Size: 8212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6403 6437 1903 Size: 34 4466 34 3678 Fragments arranged by size: 4466 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2790 4877 6104 7314 2790 Size: 2087 1227 1210 3688 Fragments arranged by size: 3688 2087 1227 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8212 BfrBI ATG'CAT Cuts at: 2841 2841 Size: 8212 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3880 4256 4668 Size: 107 122 71 631 605 2208 376 412 Cuts at: 4668 6823 136 Size: 2155 1525 Fragments arranged by size: 2208 2155 1525 631 605 412 376 122 107 71 BglII A'GATC_T Cuts at: 8207 8207 Size: 8212 BlpI GC'TnA_GC Cuts at: 5629 5629 Size: 8212 Bme1580I G_kGCm'C Cuts at: 1790 2746 2792 3647 4380 4424 4795 6273 7429 Size: 956 46 855 733 44 371 1478 1156 Cuts at: 7429 1790 Size: 2573 Fragments arranged by size: 2573 1478 1156 956 855 733 371 46 44 BmgBI CAC'GTC Cuts at: 1318 4192 5481 1318 Size: 2874 1289 4049 Fragments arranged by size: 4049 2874 1289 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4361 4782 5204 Size: 416 53 83 399 24 2944 421 422 Cuts at: 5204 5943 6901 442 Size: 739 958 1753 Fragments arranged by size: 2944 1753 958 739 422 421 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8212 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2983 4323 5284 2983 Size: 1340 961 5911 Fragments arranged by size: 5911 1340 961 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 3605 3680 3914 4609 4730 4847 6872 Size: 1211 1155 75 234 695 121 117 2025 Cuts at: 6872 1239 Size: 2579 Fragments arranged by size: 2579 2025 1211 1155 695 234 121 117 75 Bpu10I CC'TnA_GC Cuts at: 4425 4857 5012 4425 Size: 432 155 7625 Fragments arranged by size: 7625 432 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2663 6337 7202 7500 2663 Size: 3674 865 298 3375 Fragments arranged by size: 3674 3375 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2565 3411 4748 6875 882 Size: 617 1066 846 1337 2127 2219 Fragments arranged by size: 2219 2127 1337 1066 846 617 BsaAI yAC'GTr Cuts at: 493 1204 3810 4233 4351 4375 6003 493 Size: 711 2606 423 118 24 1628 2702 Fragments arranged by size: 2702 2606 1628 711 423 118 24 BsaBI GATnn'nnATC Cuts at: 8206 8206 Size: 8212 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4238 6019 6401 7036 Size: 53 83 186 936 2705 1781 382 635 Cuts at: 7036 275 Size: 1451 Fragments arranged by size: 2705 1781 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3375 3612 4451 6645 7043 7317 7464 7875 3375 Size: 237 839 2194 398 274 147 411 3712 Fragments arranged by size: 3712 2194 839 411 398 274 237 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3668 3698 4217 4247 1178 Size: 30 165 30 2265 30 519 30 5143 Fragments arranged by size: 5143 2265 519 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2048 2278 2281 2394 2763 3931 5069 Size: 853 9 230 3 113 369 1168 1138 Cuts at: 5069 5203 1186 Size: 134 4195 Fragments arranged by size: 4195 1168 1138 853 369 230 134 113 9 3 BseYI C'CCAG_C Cuts at: 2198 4130 7415 7911 2198 Size: 1932 3285 496 2499 Fragments arranged by size: 3285 2499 1932 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3674 1163 Size: 573 1938 5701 Fragments arranged by size: 5701 1938 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2312 5662 6423 7451 1540 Size: 275 186 311 3350 761 1028 2301 Fragments arranged by size: 3350 2301 1028 761 311 275 186 BsiHKAI G_wGCw'C Cuts at: 4168 6273 6358 7429 4168 Size: 2105 85 1071 4951 Fragments arranged by size: 4951 2105 1071 85 BsmI GAATG_Cn' Cuts at: 2843 4252 5723 5816 2843 Size: 1409 1471 93 5239 Fragments arranged by size: 5239 1471 1409 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3706 3706 Size: 8212 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2532 2746 2792 3647 4168 4343 4380 Size: 399 742 214 46 855 521 175 37 Cuts at: 4380 4424 4795 5036 5531 6273 6358 7429 1391 Size: 44 371 241 495 742 85 1071 2174 Fragments arranged by size: 2174 1071 855 742 742 521 495 399 371 241 214 175 85 46 44 37 BspEI T'CCGG_A Cuts at: 4451 7875 4451 Size: 3424 4788 BspHI T'CATG_A Cuts at: 4500 4875 6101 7831 4500 Size: 375 1226 1730 4881 Fragments arranged by size: 4881 1730 1226 375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2380 3294 4281 5017 5567 844 Size: 1536 914 987 736 550 3489 Fragments arranged by size: 3489 1536 987 914 736 550 BsrBI CCG'CTC Cuts at: 1394 1823 3727 3790 6099 1394 Size: 429 1904 63 2309 3507 Fragments arranged by size: 3507 2309 1904 429 63 BsrDI GCAATG_nn' Cuts at: 65 1248 4098 6707 6881 65 Size: 1183 2850 2609 174 1396 Fragments arranged by size: 2850 2609 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3879 6856 7043 1700 Size: 110 2069 2977 187 2869 Fragments arranged by size: 2977 2869 2069 187 110 BsrGI T'GTAC_A Cuts at: 96 2736 96 Size: 2640 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8212 BssSI C'ACGA_G Cuts at: 6272 7284 6272 Size: 1012 7200 BstAPI GCAn_nnn'nTGC Cuts at: 794 2605 794 Size: 1811 6401 BstBI TT'CG_AA Cuts at: 7016 7016 Size: 8212 BstEII G'GTnAC_C Cuts at: 1184 4655 1184 Size: 3471 4741 BstXI CCAn_nnnn'nTGG Cuts at: 3001 3556 3001 Size: 555 7657 BstYI r'GATC_y Cuts at: 1972 2092 2379 3606 3936 4386 5542 5909 6294 Size: 120 287 1227 330 450 1156 367 385 Cuts at: 6294 6311 7763 7849 7861 8207 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1227 1156 450 385 367 346 330 287 120 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2523 3648 4666 513 Size: 552 939 519 1125 1018 4059 Fragments arranged by size: 4059 1125 1018 939 552 519 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2221 2827 2854 3439 5089 5128 Size: 683 56 995 606 27 585 1650 39 Cuts at: 5128 487 Size: 3571 Fragments arranged by size: 3571 1650 995 683 606 585 56 39 27 BtsI GCAGTG_nn' Cuts at: 2305 3419 4756 5728 6522 6542 2305 Size: 1114 1337 972 794 20 3975 Fragments arranged by size: 3975 1337 1114 972 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8212 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2869 2904 529 Size: 35 2305 35 5837 Fragments arranged by size: 5837 2305 35 35 DraI TTT'AAA Cuts at: 5516 5516 Size: 8212 DraIII CAC_nnn'GTG Cuts at: 2143 4335 2143 Size: 2192 6020 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2549 3108 3977 7219 809 Size: 507 1233 559 869 3242 1802 Fragments arranged by size: 3242 1802 1233 869 559 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2309 3201 Size: 54 1425 50 275 18 177 302 892 Cuts at: 3201 3291 3633 3843 3872 3909 5419 5508 5659 Size: 90 342 210 29 37 1510 89 151 Cuts at: 5659 6548 8177 8 Size: 889 1629 43 Fragments arranged by size: 1629 1510 1425 892 889 342 302 275 210 177 151 90 89 54 50 43 37 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2309 5659 1537 Size: 275 497 3350 4090 Fragments arranged by size: 4090 3350 497 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4625 4691 5253 6142 1353 Size: 3272 66 562 889 3423 Fragments arranged by size: 3423 3272 889 562 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2085 2372 3569 4312 6799 7173 Size: 1064 138 496 287 1197 743 2487 374 Cuts at: 7173 7319 387 Size: 146 1280 Fragments arranged by size: 2487 1280 1197 1064 743 496 374 287 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 3605 3680 3731 3914 4503 4508 Size: 393 818 1155 75 51 183 589 5 Cuts at: 4508 4609 4628 4730 4847 5265 6275 6872 7659 Size: 101 19 102 117 418 1010 597 787 Cuts at: 7659 7927 1239 Size: 268 1524 Fragments arranged by size: 1524 1155 1010 818 787 597 589 418 393 268 183 117 102 101 75 51 19 5 EcoNI CCTnn'n_nnAGG Cuts at: 3845 3845 Size: 8212 EcoO109I rG'GnC_Cy Cuts at: 2245 2277 2683 2854 2893 2935 3889 4420 4421 Size: 32 406 171 39 42 954 531 1 Cuts at: 4421 2245 Size: 6036 Fragments arranged by size: 6036 954 531 406 171 42 39 32 1 EcoRI G'AATT_C Cuts at: 5521 5899 5521 Size: 378 7834 FspI TGC'GCA Cuts at: 6718 6718 Size: 8212 HaeII r_GCGC'y Cuts at: 1678 2454 4150 5019 7359 1678 Size: 776 1696 869 2340 2531 Fragments arranged by size: 2531 2340 1696 869 776 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2885 2917 3198 3230 4418 Size: 32 135 32 1313 32 281 32 1188 Cuts at: 4418 4450 4875 4907 4976 5008 5465 5497 6853 Size: 32 425 32 69 32 457 32 1356 Cuts at: 6853 6885 6927 6959 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 1313 1188 457 425 281 135 69 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4048 4081 5556 5802 669 Size: 988 281 2110 33 1475 246 3079 Fragments arranged by size: 3079 2110 1475 988 281 246 33 HindIII A'AGCT_T Cuts at: 748 2770 5079 748 Size: 2022 2309 3881 Fragments arranged by size: 3881 2309 2022 HpaI GTT'AAC Cuts at: 4048 5802 4048 Size: 1754 6458 KpnI G_GTAC'C Cuts at: 5537 5537 Size: 8212 MfeI C'AATT_G Cuts at: 5811 5811 Size: 8212 MluI A'CGCG_T Cuts at: 8088 8088 Size: 8212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2610 3018 3294 4027 4569 7326 7510 1305 Size: 1305 408 276 733 542 2757 184 2007 Fragments arranged by size: 2757 2007 1305 733 542 408 276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3293 3635 3845 10 Size: 54 1425 343 177 1284 342 210 4377 Fragments arranged by size: 4377 1425 1284 343 342 210 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3359 3424 3477 3650 4866 6170 6529 Size: 648 2193 65 53 173 1216 1304 359 Cuts at: 6529 6688 518 Size: 159 2042 Fragments arranged by size: 2193 2042 1304 1216 648 359 173 159 65 53 MspA1I CmG'CkG Cuts at: 1606 3658 5628 6305 7453 7698 1606 Size: 2052 1970 677 1148 245 2120 Fragments arranged by size: 2120 2052 1970 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2523 513 Size: 552 939 519 6202 Fragments arranged by size: 6202 939 552 519 NdeI CA'TA_TG Cuts at: 387 387 Size: 8212 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8212 NotI GC'GGCC_GC Cuts at: 5659 5659 Size: 8212 NsiI A_TGCA'T Cuts at: 2843 2843 Size: 8212 NspI r_CATG'y Cuts at: 1132 1229 2608 2737 5570 7115 1132 Size: 97 1379 129 2833 1545 2229 Fragments arranged by size: 2833 2229 1545 1379 129 97 PasI CC'CwG_GG Cuts at: 1348 2028 2785 4467 5113 1348 Size: 680 757 1682 646 4447 Fragments arranged by size: 4447 1682 757 680 646 PciI A'CATG_T Cuts at: 2733 7111 2733 Size: 4378 3834 PflMI CCAn_nnn'nTGG Cuts at: 1108 3148 3841 1108 Size: 2040 693 5479 Fragments arranged by size: 5479 2040 693 PfoI T'CCnGG_A Cuts at: 3222 3222 Size: 8212 PmeI GTTT'AAAC Cuts at: 5516 5516 Size: 8212 PmlI CAC'GTG Cuts at: 3810 4233 3810 Size: 423 7789 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5378 5410 6281 6313 7794 7826 5378 Size: 32 871 32 1481 32 5764 Fragments arranged by size: 5764 1481 871 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2277 2683 2854 2893 3889 2245 Size: 32 406 171 39 996 6568 Fragments arranged by size: 6568 996 406 171 39 32 PshAI GACnn'nnGTC Cuts at: 1613 4367 1613 Size: 2754 5458 PsiI TTA'TAA Cuts at: 2703 5782 2703 Size: 3079 5133 PspOMI G'GGCC_C Cuts at: 4420 4420 Size: 8212 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4523 4555 4523 Size: 32 8180 PstI C_TGCA'G Cuts at: 830 2391 2601 5307 5564 830 Size: 1561 210 2706 257 3478 Fragments arranged by size: 3478 2706 1561 257 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8212 PvuII CAG'CTG Cuts at: 3658 3658 Size: 8212 SalI G'TCGA_C Cuts at: 1936 5554 1936 Size: 3618 4594 SanDI GG'GwC_CC Cuts at: 2277 2854 2277 Size: 577 7635 SbfI CC_TGCA'GG Cuts at: 2391 5307 5564 2391 Size: 2916 257 5039 Fragments arranged by size: 5039 2916 257 ScaI AGT'ACT Cuts at: 6460 8172 6460 Size: 1712 6500 SexAI A'CCwGG_T Cuts at: 3627 3627 Size: 8212 SfcI C'TryA_G Cuts at: 826 1045 2387 2597 2617 4693 5278 5303 5560 Size: 219 1342 210 20 2076 585 25 257 Cuts at: 5560 6695 7376 7567 826 Size: 1135 681 191 1471 Fragments arranged by size: 2076 1471 1342 1135 681 585 257 219 210 191 25 20 SfiI GGCCn_nnn'nGGCC Cuts at: 3880 3880 Size: 8212 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8212 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8212 SmaI CCC'GGG Cuts at: 1872 2252 3887 3943 5539 1872 Size: 380 1635 56 1596 4545 Fragments arranged by size: 4545 1635 1596 380 56 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 2716 4308 6316 7217 7479 Size: 197 926 735 38 1592 2008 901 262 Cuts at: 7479 820 Size: 1553 Fragments arranged by size: 2008 1592 1553 926 901 735 262 197 38 SnaBI TAC'GTA Cuts at: 493 6003 493 Size: 5510 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8212 SphI G_CATG'C Cuts at: 1132 5570 1132 Size: 4438 3774 SspI AAT'ATT Cuts at: 5 52 6136 5 Size: 47 6084 2081 Fragments arranged by size: 6084 2081 47 StuI AGG'CCT Cuts at: 1517 3851 4316 1517 Size: 2334 465 5413 Fragments arranged by size: 5413 2334 465 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2415 2523 3846 4758 4796 Size: 552 939 237 174 108 1323 912 38 Cuts at: 4796 5236 5253 5651 513 Size: 440 17 398 3074 Fragments arranged by size: 3074 1323 939 912 552 440 398 237 174 108 38 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2552 2627 2660 3245 3917 4609 5126 Size: 1255 972 75 33 585 672 692 517 Cuts at: 5126 6239 6256 6409 6594 7039 325 Size: 1113 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1113 972 692 672 585 517 445 185 153 75 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2736 5424 6458 Size: 274 80 33 51 1443 759 2688 1034 Cuts at: 6458 8170 96 Size: 1712 138 Fragments arranged by size: 2688 1712 1443 1034 759 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 3668 3700 4217 4249 8172 8204 2321 Size: 32 1315 32 517 32 3923 32 2329 Fragments arranged by size: 3923 2329 1315 517 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2432 3974 4550 1316 Size: 1116 1542 576 4978 Fragments arranged by size: 4978 1542 1116 576 XbaI T'CTAG_A Cuts at: 5548 5548 Size: 8212 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2025 2871 3553 3655 3778 4004 5026 5149 2025 Size: 846 682 102 123 226 1022 123 5088 Fragments arranged by size: 5088 1022 846 682 226 123 123 102 XhoI C'TCGA_G Cuts at: 1943 4308 1943 Size: 2365 5847 XmaI C'CCGG_G Cuts at: 1870 2250 3885 3941 5537 1870 Size: 380 1635 56 1596 4545 Fragments arranged by size: 4545 1635 1596 380 56 XmnI GAAnn'nnTTC Cuts at: 2669 6341 2669 Size: 3672 4540 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6020 276 Size: 53 83 186 936 4486 2468 Fragments arranged by size: 4486 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI RsrII SacI SacII SapI SfoI SrfI SwaI