Restriction map for
pFN21AA0756
(Linear) (Six-base) MAPSORT of: KIAA0756.seq Check: 79 from: 1 to: 3507
KIAA0756.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 375 3012 3507
Size: 375 2637 495
Fragments arranged by size:
2637 495 375
AccI GT'mk_AC
Cuts at: 0 2366 2534 3507
Size: 2366 168 973
Fragments arranged by size:
2366 973 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1726 2498 2503 2623 3260 3507
Size: 1726 772 5 120 637 247
Fragments arranged by size:
1726 772 637 247 120 5
AfeI AGC'GCT
Cuts at: 0 2143 3507
Size: 2143 1364
AflII C'TTAA_G
Cuts at: 0 711 3507
Size: 711 2796
AflIII A'CryG_T
Cuts at: 0 543 728 1802 1804 3507
Size: 543 185 1074 2 1703
Fragments arranged by size:
1703 1074 543 185 2
AhdI GACnn_n'nnGTC
Cuts at: 0 2985 3507
Size: 2985 522
AleI CACnn'nnGTG
Cuts at: 0 1645 3507
Size: 1645 1862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1663 3507
Size: 1663 1844
ApaI G_GGCC'C
Cuts at: 0 2419 3507
Size: 2419 1088
ApoI r'AATT_y
Cuts at: 0 77 349 3507
Size: 77 272 3158
Fragments arranged by size:
3158 272 77
AvaI C'yCGr_G
Cuts at: 0 245 740 1615 1880 1936 2167 2303 3507
Size: 245 495 875 265 56 231 136 1204
Fragments arranged by size:
1204 875 495 265 245 231 136 56
AvrII C'CTAG_G
Cuts at: 0 2791 3507
Size: 2791 716
BamHI G'GATC_C
Cuts at: 0 374 1931 3507
Size: 374 1557 1576
Fragments arranged by size:
1576 1557 374
BanI G'GyrC_C
Cuts at: 0 784 1170 1639 3325 3507
Size: 784 386 469 1686 182
Fragments arranged by size:
1686 784 469 386 182
BanII G_rGCy'C
Cuts at: 0 527 2338 2419 3031 3507
Size: 527 1811 81 612 476
Fragments arranged by size:
1811 612 527 476 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 1757 1994 3248 3404 3507
Size: 965 792 237 1254 156 103
Fragments arranged by size:
1254 965 792 237 156 103
BbvCI CC'TCA_GC
Cuts at: 0 3007 3507
Size: 3007 500
BciVI GTATCCnnnnn_n'
Cuts at: 0 785 2872 3507
Size: 785 2087 635
Fragments arranged by size:
2087 785 635
BfrBI ATG'CAT
Cuts at: 0 836 3507
Size: 836 2671
BglI GCCn_nnn'nGGC
Cuts at: 0 1875 2251 2663 3507
Size: 1875 376 412 844
Fragments arranged by size:
1875 844 412 376
Bme1580I G_kGCm'C
Cuts at: 0 741 787 1642 2375 2419 2790 3507
Size: 741 46 855 733 44 371 717
Fragments arranged by size:
855 741 733 717 371 46 44
BmgBI CAC'GTC
Cuts at: 0 2187 3476 3507
Size: 2187 1289 31
Fragments arranged by size:
2187 1289 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 2356 2777 3199 3507
Size: 2356 421 422 308
Fragments arranged by size:
2356 422 421 308
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 978 2318 3279 3507
Size: 978 1340 961 228
Fragments arranged by size:
1340 978 961 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1600 1675 1909 2604 2725 2842 3507
Size: 445 1155 75 234 695 121 117 665
Fragments arranged by size:
1155 695 665 445 234 121 117 75
Bpu10I CC'TnA_GC
Cuts at: 0 2420 2852 3007 3507
Size: 2420 432 155 500
Fragments arranged by size:
2420 500 432 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 3507
Size: 658 2849
BsaI GGTCTCn'nnnn_
Cuts at: 0 560 1406 2743 3507
Size: 560 846 1337 764
Fragments arranged by size:
1337 846 764 560
BsaAI yAC'GTr
Cuts at: 0 1805 2228 2346 2370 3507
Size: 1805 423 118 24 1137
Fragments arranged by size:
1805 1137 423 118 24
BsaHI Gr'CG_yC
Cuts at: 0 2233 3507
Size: 2233 1274
BsaWI w'CCGG_w
Cuts at: 0 1370 1607 2446 3507
Size: 1370 237 839 1061
Fragments arranged by size:
1370 1061 839 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1663 1693 2212 2242 3507
Size: 1663 30 519 30 1265
Fragments arranged by size:
1663 1265 519 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 43 273 276 389 758 1926 3064
Size: 34 9 230 3 113 369 1168 1138
Cuts at: 3064 3198 3507
Size: 134 309
Fragments arranged by size:
1168 1138 369 309 230 134 113 34
9 3
BseYI C'CCAG_C
Cuts at: 0 193 2125 3507
Size: 193 1932 1382
Fragments arranged by size:
1932 1382 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 3507
Size: 1669 1838
BsiEI CG_ry'CG
Cuts at: 0 307 3507
Size: 307 3200
BsiHKAI G_wGCw'C
Cuts at: 0 2163 3507
Size: 2163 1344
BsmI GAATG_Cn'
Cuts at: 0 838 2247 3507
Size: 838 1409 1260
Fragments arranged by size:
1409 1260 838
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1701 3507
Size: 1701 1806
Bsp1286I G_dGCh'C
Cuts at: 0 527 741 787 1642 2163 2338 2375 2419
Size: 527 214 46 855 521 175 37 44
Cuts at: 2419 2790 3031 3507
Size: 371 241 476
Fragments arranged by size:
855 527 521 476 371 241 214 175
46 44 37
BspEI T'CCGG_A
Cuts at: 0 2446 3507
Size: 2446 1061
BspHI T'CATG_A
Cuts at: 0 2495 2870 3507
Size: 2495 375 637
Fragments arranged by size:
2495 637 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 375 1289 2276 3012 3507
Size: 375 914 987 736 495
Fragments arranged by size:
987 914 736 495 375
BsrBI CCG'CTC
Cuts at: 0 1722 1785 3507
Size: 1722 63 1722
Fragments arranged by size:
1722 1722 63
BsrDI GCAATG_nn'
Cuts at: 0 2093 3507
Size: 2093 1414
BsrFI r'CCGG_y
Cuts at: 0 1874 3507
Size: 1874 1633
BsrGI T'GTAC_A
Cuts at: 0 731 3507
Size: 731 2776
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 3507
Size: 600 2907
BstEII G'GTnAC_C
Cuts at: 0 2650 3507
Size: 2650 857
BstXI CCAn_nnnn'nTGG
Cuts at: 0 996 1551 3507
Size: 996 555 1956
Fragments arranged by size:
1956 996 555
BstYI r'GATC_y
Cuts at: 0 87 374 1601 1931 2381 3507
Size: 87 287 1227 330 450 1126
Fragments arranged by size:
1227 1126 450 330 287 87
BtgI C'CryG_G
Cuts at: 0 518 1643 2661 3507
Size: 518 1125 1018 846
Fragments arranged by size:
1125 1018 846 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 216 822 849 1434 3084 3123 3507
Size: 216 606 27 585 1650 39 384
Fragments arranged by size:
1650 606 585 384 216 39 27
BtsI GCAGTG_nn'
Cuts at: 0 300 1414 2751 3507
Size: 300 1114 1337 756
Fragments arranged by size:
1337 1114 756 300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 864 899 3507
Size: 864 35 2608
Fragments arranged by size:
2608 864 35
DraIII CAC_nnn'GTG
Cuts at: 0 138 2330 3507
Size: 138 2192 1177
Fragments arranged by size:
2192 1177 138
DrdI GACnn_nn'nnGTC
Cuts at: 0 544 1103 1972 3507
Size: 544 559 869 1535
Fragments arranged by size:
1535 869 559 544
EaeI y'GGCC_r
Cuts at: 0 2 304 1196 1286 1628 1838 1867 1904
Size: 2 302 892 90 342 210 29 37
Cuts at: 1904 3414 3507
Size: 1510 93
Fragments arranged by size:
1510 892 342 302 210 93 90 37
29 2
EagI C'GGCC_G
Cuts at: 0 304 3507
Size: 304 3203
EarI CTCTTCn'nnn_
Cuts at: 0 2620 2686 3248 3507
Size: 2620 66 562 259
Fragments arranged by size:
2620 562 259 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 367 1564 2307 3507
Size: 80 287 1197 743 1200
Fragments arranged by size:
1200 1197 743 287 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1600 1675 1726 1909 2498 2503 2604
Size: 445 1155 75 51 183 589 5 101
Cuts at: 2604 2623 2725 2842 3260 3507
Size: 19 102 117 418 247
Fragments arranged by size:
1155 589 445 418 247 183 117 102
101 75 51 19 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1840 3507
Size: 1840 1667
EcoO109I rG'GnC_Cy
Cuts at: 0 240 272 678 849 888 930 1884 2415
Size: 240 32 406 171 39 42 954 531
Cuts at: 2415 2416 3507
Size: 1 1091
Fragments arranged by size:
1091 954 531 406 240 171 42 39
32 1
HaeII r_GCGC'y
Cuts at: 0 449 2145 3014 3507
Size: 449 1696 869 493
Fragments arranged by size:
1696 869 493 449
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 880 912 1193 1225 2413 2445 2870 2902
Size: 880 32 281 32 1188 32 425 32
Cuts at: 2902 2971 3003 3460 3492 3507
Size: 69 32 457 32 15
Fragments arranged by size:
1188 880 457 425 281 69 32 32
32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 2043 2076 3507
Size: 2043 33 1431
Fragments arranged by size:
2043 1431 33
HindIII A'AGCT_T
Cuts at: 0 765 3074 3507
Size: 765 2309 433
Fragments arranged by size:
2309 765 433
HpaI GTT'AAC
Cuts at: 0 2043 3507
Size: 2043 1464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1013 1289 2022 2564 3507
Size: 605 408 276 733 542 943
Fragments arranged by size:
943 733 605 542 408 276
MscI TGG'CCA
Cuts at: 0 4 1288 1630 1840 3507
Size: 4 1284 342 210 1667
Fragments arranged by size:
1667 1284 342 210 4
MslI CAynn'nnrTG
Cuts at: 0 1354 1419 1472 1645 2861 3507
Size: 1354 65 53 173 1216 646
Fragments arranged by size:
1354 1216 646 173 65 53
MspA1I CmG'CkG
Cuts at: 0 1653 3507
Size: 1653 1854
NcoI C'CATG_G
Cuts at: 0 518 3507
Size: 518 2989
NsiI A_TGCA'T
Cuts at: 0 838 3507
Size: 838 2669
NspI r_CATG'y
Cuts at: 0 603 732 3507
Size: 603 129 2775
Fragments arranged by size:
2775 603 129
PasI CC'CwG_GG
Cuts at: 0 23 780 2462 3108 3507
Size: 23 757 1682 646 399
Fragments arranged by size:
1682 757 646 399 23
PciI A'CATG_T
Cuts at: 0 728 3507
Size: 728 2779
PflMI CCAn_nnn'nTGG
Cuts at: 0 1143 1836 3507
Size: 1143 693 1671
Fragments arranged by size:
1671 1143 693
PfoI T'CCnGG_A
Cuts at: 0 1217 3507
Size: 1217 2290
PmlI CAC'GTG
Cuts at: 0 1805 2228 3507
Size: 1805 423 1279
Fragments arranged by size:
1805 1279 423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3373 3405 3507
Size: 3373 32 102
Fragments arranged by size:
3373 102 32
PpuMI rG'GwC_Cy
Cuts at: 0 240 272 678 849 888 1884 3507
Size: 240 32 406 171 39 996 1623
Fragments arranged by size:
1623 996 406 240 171 39 32
PshAI GACnn'nnGTC
Cuts at: 0 2362 3507
Size: 2362 1145
PsiI TTA'TAA
Cuts at: 0 698 3507
Size: 698 2809
PspOMI G'GGCC_C
Cuts at: 0 2415 3507
Size: 2415 1092
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2518 2550 3507
Size: 2518 32 957
Fragments arranged by size:
2518 957 32
PstI C_TGCA'G
Cuts at: 0 386 596 3302 3507
Size: 386 210 2706 205
Fragments arranged by size:
2706 386 210 205
PvuII CAG'CTG
Cuts at: 0 1653 3507
Size: 1653 1854
SanDI GG'GwC_CC
Cuts at: 0 272 849 3507
Size: 272 577 2658
Fragments arranged by size:
2658 577 272
SbfI CC_TGCA'GG
Cuts at: 0 386 3302 3507
Size: 386 2916 205
Fragments arranged by size:
2916 386 205
SexAI A'CCwGG_T
Cuts at: 0 1622 3507
Size: 1622 1885
SfcI C'TryA_G
Cuts at: 0 382 592 612 2688 3273 3298 3507
Size: 382 210 20 2076 585 25 209
Fragments arranged by size:
2076 585 382 210 209 25 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1875 3507
Size: 1875 1632
SmaI CCC'GGG
Cuts at: 0 247 1882 1938 3507
Size: 247 1635 56 1569
Fragments arranged by size:
1635 1569 247 56
SmlI C'TyrA_G
Cuts at: 0 673 711 2303 3507
Size: 673 38 1592 1204
Fragments arranged by size:
1592 1204 673 38
StuI AGG'CCT
Cuts at: 0 1846 2311 3507
Size: 1846 465 1196
Fragments arranged by size:
1846 1196 465
StyI C'CwwG_G
Cuts at: 0 236 410 518 1841 2753 2791 3231 3248
Size: 236 174 108 1323 912 38 440 17
Cuts at: 3248 3507
Size: 259
Fragments arranged by size:
1323 912 440 259 236 174 108 38
17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 547 622 655 1240 1912 2604 3121 3507
Size: 547 75 33 585 672 692 517 386
Fragments arranged by size:
692 672 585 547 517 386 75 33
TatI w'GTAC_w
Cuts at: 0 731 3419 3507
Size: 731 2688 88
Fragments arranged by size:
2688 731 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 1663 1695 2212 2244 3507
Size: 316 32 1315 32 517 32 1263
Fragments arranged by size:
1315 1263 517 316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 427 1969 2545 3507
Size: 427 1542 576 962
Fragments arranged by size:
1542 962 576 427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 20 866 1548 1650 1773 1999 3021 3144
Size: 20 846 682 102 123 226 1022 123
Cuts at: 3144 3507
Size: 363
Fragments arranged by size:
1022 846 682 363 226 123 123 102
20
XhoI C'TCGA_G
Cuts at: 0 2303 3507
Size: 2303 1204
XmaI C'CCGG_G
Cuts at: 0 245 1880 1936 3507
Size: 245 1635 56 1571
Fragments arranged by size:
1635 1571 245 56
XmnI GAAnn'nnTTC
Cuts at: 0 664 3507
Size: 664 2843
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AleI
AlfI ApaI ApoI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BciVI BfrBI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII HindIII HpaI MmeI MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuII SanDI SbfI SexAI SfcI SfiI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AloI AlwNI ApaLI AscI
AseI AsiSI BaeI BbeI BcgI BclI BglII BlpI
BmtI BsaBI BsiWI BssHII BssSI BstBI BstZ17I Bsu36I
ClaI DraI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PmeI PspXI PvuI
RsrII SacI SacII SalI SapI ScaI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
ZraI