Restriction map for
pFN21AA0706
(Circular) (Six-base) MAPSORT of: pFN21AA0706.seq Check: 7105 from: 1 to: 8014
pFN21AA0706 8014 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5824 278
Size: 53 83 186 936 4288 2468
Fragments arranged by size:
4288 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3870 5357 1937
Size: 1933 1487 4594
Fragments arranged by size:
4594 1933 1487
Acc65I G'GTAC_C
Cuts at: 5335 5335
Size: 8014
AclI AA'CG_TT
Cuts at: 1563 6141 6514 1563
Size: 4578 373 3063
Fragments arranged by size:
4578 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2819 3194 3767 4265 4704 6077 7461 7729
Size: 1187 375 573 498 439 1373 1384 268
Cuts at: 7729 1632
Size: 1917
Fragments arranged by size:
1917 1384 1373 1187 573 498 439 375
268
AfeI AGC'GCT
Cuts at: 2687 4052 2687
Size: 1365 6649
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7817
AflIII A'CryG_T
Cuts at: 1203 2274 6913 7890 1203
Size: 1071 4639 977 1327
Fragments arranged by size:
4639 1327 1071 977
AgeI A'CCGG_T
Cuts at: 6845 6845
Size: 8014
AhdI GACnn_n'nnGTC
Cuts at: 6743 6743
Size: 8014
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5295 1184
Size: 4111 3903
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3819 3851 3819
Size: 32 7982
AlwNI CAG_nnn'CTG
Cuts at: 2545 3148 3158 3952 4012 4585 4858 5757 7329
Size: 603 10 794 60 573 273 899 1572
Cuts at: 7329 2545
Size: 3230
Fragments arranged by size:
3230 1572 899 794 603 573 273 60
10
ApaI G_GGCC'C
Cuts at: 2531 2785 4168 4510 4678 5232 2531
Size: 254 1383 342 168 554 5313
Fragments arranged by size:
5313 1383 554 342 254 168
ApaLI G'TGCA_C
Cuts at: 6071 7227 6071
Size: 1156 6858
ApoI r'AATT_y
Cuts at: 1493 2938 5323 5549 5701 7810 1493
Size: 1445 2385 226 152 2109 1697
Fragments arranged by size:
2385 2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6568 160
Size: 872 5536 1606
Fragments arranged by size:
5536 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8014
AvaI C'yCGr_G
Cuts at: 1870 1943 2784 4404 4479 5124 5339 1870
Size: 73 841 1620 75 645 215 4545
Fragments arranged by size:
4545 1620 841 645 215 75 73
BamHI G'GATC_C
Cuts at: 5344 5711 5344
Size: 367 7647
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 3200 5335 6790 618
Size: 325 217 627 225 1188 2135 1455 1842
Fragments arranged by size:
2135 1842 1455 1188 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 2531 2785 2984 4168 4480 4510 4678 4868
Size: 1140 254 199 1184 312 30 168 190
Cuts at: 4868 5232 5333 1391
Size: 364 101 4072
Fragments arranged by size:
4072 1184 1140 364 312 254 199 190
168 101 30
BbeI G_GCGC'C
Cuts at: 3204 3204
Size: 8014
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2668 3182 3472 3500 3528 4811 928
Size: 971 769 514 290 28 28 1283 4131
Fragments arranged by size:
4131 1283 971 769 514 290 28 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2207 2241 6205 6239 1903
Size: 34 270 34 3964 34 3678
Fragments arranged by size:
3964 3678 270 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5906 7116 5906
Size: 1210 6804
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8014
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4235 4394 4484
Size: 107 122 71 631 605 2563 159 90
Cuts at: 4484 5227 6625 136
Size: 743 1398 1525
Fragments arranged by size:
2563 1525 1398 743 631 605 159 122
107 90 71
BglII A'GATC_T
Cuts at: 2448 4266 8009 2448
Size: 1818 3743 2453
Fragments arranged by size:
3743 2453 1818
BlpI GC'TnA_GC
Cuts at: 2412 3360 5431 2412
Size: 948 2071 4995
Fragments arranged by size:
4995 2071 948
Bme1580I G_kGCm'C
Cuts at: 1790 2364 2531 2785 4168 4510 4678 4917 5232
Size: 574 167 254 1383 342 168 239 315
Cuts at: 5232 6075 7231 1790
Size: 843 1156 2573
Fragments arranged by size:
2573 1383 1156 843 574 342 315 254
239 168 167
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8014
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2194 4710 5745
Size: 416 53 83 399 24 777 2516 1035
Cuts at: 5745 6703 442
Size: 958 1753
Fragments arranged by size:
2516 1753 1035 958 777 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3216 3293 3704 3920 3974 4019 4090 4411
Size: 1977 77 411 216 54 45 71 321
Cuts at: 4411 6674 1239
Size: 2263 2579
Fragments arranged by size:
2579 2263 1977 411 321 216 77 71
54 45
Bpu10I CC'TnA_GC
Cuts at: 2978 3141 2978
Size: 163 7851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 4319 4814 5276 6139 7004 7302 2915
Size: 1404 495 462 863 865 298 3627
Fragments arranged by size:
3627 1404 865 863 495 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 2463 2633 6677 882
Size: 617 555 409 170 4044 2219
Fragments arranged by size:
4044 2219 617 555 409 170
BsaAI yAC'GTr
Cuts at: 493 1204 2536 5805 493
Size: 711 1332 3269 2702
Fragments arranged by size:
3269 2702 1332 711
BsaBI GATnn'nnATC
Cuts at: 8008 8008
Size: 8014
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3201 3477 3567 4386
Size: 53 83 186 936 1668 276 90 819
Cuts at: 4386 5821 6203 6838 275
Size: 1435 382 635 1451
Fragments arranged by size:
1668 1451 1435 936 819 635 382 276
186 90 83 53
BsaWI w'CCGG_w
Cuts at: 3608 3642 3966 6447 6845 7119 7266 7677 3608
Size: 34 324 2481 398 274 147 411 3945
Fragments arranged by size:
3945 2481 411 398 324 274 147 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 3657 3687 3947
Size: 30 165 30 690 30 1534 30 260
Cuts at: 3947 3977 4760 4790 1178
Size: 30 783 30 4402
Fragments arranged by size:
4402 1534 783 690 260 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2764 3017 3395 4072 4445 4484 4718 4805
Size: 1578 253 378 677 373 39 234 87
Cuts at: 4805 5039 1186
Size: 234 4161
Fragments arranged by size:
4161 1578 677 378 373 253 234 234
87 39
BseYI C'CCAG_C
Cuts at: 2306 2364 2753 3226 3654 4145 4191 4224 4764
Size: 58 389 473 428 491 46 33 540
Cuts at: 4764 4900 4959 5154 5190 5209 5214 7217 7713
Size: 136 59 195 36 19 5 2003 496
Cuts at: 7713 2306
Size: 2607
Fragments arranged by size:
2607 2003 540 496 491 473 428 389
195 136 59 58 46 36 33 19
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2558 5124 1163
Size: 573 822 2566 4053
Fragments arranged by size:
4053 2566 822 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5464 6225 7253 1540
Size: 275 186 3463 761 1028 2301
Fragments arranged by size:
3463 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2413 2517 2919 6075 6160 7231 2413
Size: 104 402 3156 85 1071 3196
Fragments arranged by size:
3196 3156 1071 402 104 85
BsiWI C'GTAC_G
Cuts at: 2532 2532
Size: 8014
BsmI GAATG_Cn'
Cuts at: 5525 5618 5525
Size: 93 7921
BsmBI CGTCTCn'nnnn_
Cuts at: 4377 5260 4377
Size: 883 7131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2413 2517 2531 2785 2919 2984
Size: 399 574 49 104 14 254 134 65
Cuts at: 2984 4168 4480 4510 4678 4868 4917 5232 5333
Size: 1184 312 30 168 190 49 315 101
Cuts at: 5333 6075 6160 7231 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1184 1071 742 574 399 315 312
254 190 168 134 104 101 85 65
49 49 30 14
BspEI T'CCGG_A
Cuts at: 3642 7677 3642
Size: 4035 3979
BspHI T'CATG_A
Cuts at: 4636 5903 7633 4636
Size: 1267 1730 5017
Fragments arranged by size:
5017 1730 1267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5369 844
Size: 4525 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2461 4771 4992 5011 5132 5901 1394
Size: 429 638 2310 221 19 121 769 3507
Fragments arranged by size:
3507 2310 769 638 429 221 121 19
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 3061 3425 6509 6683 65
Size: 1183 1329 484 364 3084 174 1396
Fragments arranged by size:
3084 1396 1329 1183 484 364 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5058 6658 6845 1700
Size: 110 3248 1600 187 2869
Fragments arranged by size:
3248 2869 1600 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8014
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8014
BssSI C'ACGA_G
Cuts at: 6074 7086 6074
Size: 1012 7002
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2579 4585 4858 794
Size: 1785 2006 273 3950
Fragments arranged by size:
3950 2006 1785 273
BstBI TT'CG_AA
Cuts at: 6818 6818
Size: 8014
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8014
BstXI CCAn_nnnn'nTGG
Cuts at: 2083 3662 3873 2083
Size: 1579 211 6224
Fragments arranged by size:
6224 1579 211
BstYI r'GATC_y
Cuts at: 1972 2448 3949 4185 4266 5344 5711 6096 6113
Size: 476 1501 236 81 1078 367 385 17
Cuts at: 6113 7565 7651 7663 8009 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1501 1452 1078 476 385 367 346
236 86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2776 3020 3315 3407 5022 2776
Size: 244 295 92 1615 5768
Fragments arranged by size:
5768 1615 295 244 92
BtgI C'CryG_G
Cuts at: 513 1065 2004 3324 4110 4233 4300 513
Size: 552 939 1320 786 123 67 4227
Fragments arranged by size:
4227 1320 939 786 552 123 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7275
Fragments arranged by size:
7275 683 56
BtsI GCAGTG_nn'
Cuts at: 2037 4156 4459 4471 5530 6324 6344 2037
Size: 2119 303 12 1059 794 20 3707
Fragments arranged by size:
3707 2119 1059 794 303 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4117 4152 5281 5316 529
Size: 35 3553 35 1129 35 3227
Fragments arranged by size:
3553 3227 1129 35 35 35
DraI TTT'AAA
Cuts at: 5318 5318
Size: 8014
DraIII CAC_nnn'GTG
Cuts at: 3508 3508
Size: 8014
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7021 809
Size: 507 5705 1802
Fragments arranged by size:
5705 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3447 3580 4326
Size: 54 1425 50 275 18 1617 133 746
Cuts at: 4326 4976 5461 6350 7979 8
Size: 650 485 889 1629 43
Fragments arranged by size:
1629 1617 1425 889 746 650 485 275
133 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5461 1537
Size: 275 3649 4090
Fragments arranged by size:
4090 3649 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2387 3932 4056 5944 1353
Size: 882 152 1545 124 1888 3423
Fragments arranged by size:
3423 1888 1545 882 152 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4286 4502 6601 6975 7121 387
Size: 1064 138 2697 216 2099 374 146 1280
Fragments arranged by size:
2697 2099 1280 1064 374 216 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2819 3194 3216 3293 3704 3767 3920
Size: 393 1187 375 22 77 411 63 153
Cuts at: 3920 3974 4019 4090 4265 4411 4704 6077 6674
Size: 54 45 71 175 146 293 1373 597
Cuts at: 6674 7461 7729 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1373 1187 787 597 411 393 375
293 268 175 153 146 77 71 63
54 45 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3693 4628 3693
Size: 935 7079
EcoO109I rG'GnC_Cy
Cuts at: 2194 2377 2542 2781 3073 3835 4164 4165 4321
Size: 183 165 239 292 762 329 1 156
Cuts at: 4321 4498 4582 4666 4675 4795 4896 5079 5228
Size: 177 84 84 9 120 101 183 149
Cuts at: 5228 5229 2194
Size: 1 4979
Fragments arranged by size:
4979 762 329 292 239 183 183 177
165 156 149 120 101 84 84 9
1 1
EcoRI G'AATT_C
Cuts at: 5323 5701 5323
Size: 378 7636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4007 4954 4007
Size: 947 7067
FspI TGC'GCA
Cuts at: 4892 6520 4892
Size: 1628 6386
HaeII r_GCGC'y
Cuts at: 1678 2689 3204 4054 7161 1678
Size: 1011 515 850 3107 2531
Fragments arranged by size:
3107 2531 1011 850 515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2799 2831 2855
Size: 32 135 32 797 32 398 32 24
Cuts at: 2855 2887 4037 4069 4760 4792 6655 6687 6729
Size: 32 1150 32 691 32 1863 32 42
Cuts at: 6729 6761 1373
Size: 32 2626
Fragments arranged by size:
2626 1863 1150 797 691 398 135 42
32 32 32 32 32 32 32 32
32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 3871 5358 5604 669
Size: 988 281 1933 1487 246 3079
Fragments arranged by size:
3079 1933 1487 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2149 3722 748
Size: 1401 1573 5040
Fragments arranged by size:
5040 1573 1401
HpaI GTT'AAC
Cuts at: 5604 5604
Size: 8014
KasI G'GCGC_C
Cuts at: 3200 3200
Size: 8014
KpnI G_GTAC'C
Cuts at: 5339 5339
Size: 8014
MfeI C'AATT_G
Cuts at: 5613 5613
Size: 8014
MluI A'CGCG_T
Cuts at: 7890 7890
Size: 8014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 5027 5038 7128 7312 1305
Size: 1563 2159 11 2090 184 2007
Fragments arranged by size:
2159 2090 2007 1563 184 11
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4978 10
Size: 54 1425 343 3146 3046
Fragments arranged by size:
3146 3046 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 2963 4641 5972 6331 6490 518
Size: 648 1464 333 1678 1331 359 159 2042
Fragments arranged by size:
2042 1678 1464 1331 648 359 333 159
MspA1I CmG'CkG
Cuts at: 1606 3051 3212 3739 4032 4099 4224 5218 5309
Size: 1445 161 527 293 67 125 994 91
Cuts at: 5309 5430 6107 7255 7500 1606
Size: 121 677 1148 245 2120
Fragments arranged by size:
2120 1445 1148 994 677 527 293 245
161 125 121 91 67
NaeI GCC'GGC
Cuts at: 5060 5060
Size: 8014
NarI GG'CG_CC
Cuts at: 3201 3201
Size: 8014
NcoI C'CATG_G
Cuts at: 513 1065 2004 3324 4233 513
Size: 552 939 1320 909 4294
Fragments arranged by size:
4294 1320 939 909 552
NdeI CA'TA_TG
Cuts at: 387 3709 387
Size: 3322 4692
NgoMIV G'CCGG_C
Cuts at: 5058 5058
Size: 8014
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8014
NotI GC'GGCC_GC
Cuts at: 5461 5461
Size: 8014
NspI r_CATG'y
Cuts at: 1132 1229 2095 2794 4600 4729 5372 6917 1132
Size: 97 866 699 1806 129 643 1545 2229
Fragments arranged by size:
2229 1806 1545 866 699 643 129 97
PasI CC'CwG_GG
Cuts at: 1348 4671 5225 1348
Size: 3323 554 4137
Fragments arranged by size:
4137 3323 554
PciI A'CATG_T
Cuts at: 6913 6913
Size: 8014
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8014
PfoI T'CCnGG_A
Cuts at: 2508 3453 2508
Size: 945 7069
PmeI GTTT'AAAC
Cuts at: 5318 5318
Size: 8014
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2511 6083 6115 7596 7628 2479
Size: 32 3572 32 1481 32 2865
Fragments arranged by size:
3572 2865 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2377 2542 3073 3835 4498 4666 4795 5079
Size: 183 165 531 762 663 168 129 284
Cuts at: 5079 2194
Size: 5129
Fragments arranged by size:
5129 762 663 531 284 183 168 165
129
PshAI GACnn'nnGTC
Cuts at: 1613 4124 1613
Size: 2511 5503
PsiI TTA'TAA
Cuts at: 5584 5584
Size: 8014
PspOMI G'GGCC_C
Cuts at: 2527 2781 4164 4506 4674 5228 2527
Size: 254 1383 342 168 554 5313
Fragments arranged by size:
5313 1383 554 342 254 168
PstI C_TGCA'G
Cuts at: 830 3105 3339 3948 4209 4539 4554 4581 4854
Size: 2275 234 609 261 330 15 27 273
Cuts at: 4854 4863 5366 830
Size: 9 503 3478
Fragments arranged by size:
3478 2275 609 503 330 273 261 234
27 15 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8014
PvuII CAG'CTG
Cuts at: 3212 4099 5309 3212
Size: 887 1210 5917
Fragments arranged by size:
5917 1210 887
SalI G'TCGA_C
Cuts at: 1936 3869 5356 1936
Size: 1933 1487 4594
Fragments arranged by size:
4594 1933 1487
SanDI GG'GwC_CC
Cuts at: 3835 3835
Size: 8014
SbfI CC_TGCA'GG
Cuts at: 5366 5366
Size: 8014
ScaI AGT'ACT
Cuts at: 6262 7974 6262
Size: 1712 6302
SexAI A'CCwGG_T
Cuts at: 3504 3504
Size: 8014
SfcI C'TryA_G
Cuts at: 826 1045 2902 3101 3335 3944 4205 4422 4535
Size: 219 1857 199 234 609 261 217 113
Cuts at: 4535 4550 4577 4850 4859 5362 6497 7178 7369
Size: 15 27 273 9 503 1135 681 191
Cuts at: 7369 826
Size: 1471
Fragments arranged by size:
1857 1471 1135 681 609 503 273 261
234 219 217 199 191 113 27 15
9
SfiI GGCCn_nnn'nGGCC
Cuts at: 5227 5227
Size: 8014
SfoI GGC'GCC
Cuts at: 3202 3202
Size: 8014
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8014
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8014
SmaI CCC'GGG
Cuts at: 1872 2786 4406 5126 5341 1872
Size: 914 1620 720 215 4545
Fragments arranged by size:
4545 1620 914 720 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2930 4334 4829 5291 6118 7019
Size: 197 926 987 1404 495 462 827 901
Cuts at: 7019 7281 820
Size: 262 1553
Fragments arranged by size:
1553 1404 987 926 901 827 495 462
262 197
SnaBI TAC'GTA
Cuts at: 493 5805 493
Size: 5312 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8014
SphI G_CATG'C
Cuts at: 1132 2095 2794 4600 4729 5372 1132
Size: 963 699 1806 129 643 3774
Fragments arranged by size:
3774 1806 963 699 643 129
SspI AAT'ATT
Cuts at: 5 52 5938 5
Size: 47 5886 2081
Fragments arranged by size:
5886 2081 47
StuI AGG'CCT
Cuts at: 1517 3171 1517
Size: 1654 6360
StyI C'CwwG_G
Cuts at: 513 1065 2004 3324 4233 5453 513
Size: 552 939 1320 909 1220 3074
Fragments arranged by size:
3074 1320 1220 939 909 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3265 6041 6058 6211 6396 6841 325
Size: 1255 1685 2776 17 153 185 445 1498
Fragments arranged by size:
2776 1685 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6260 7972 96
Size: 274 80 33 51 1443 4283 1712 138
Fragments arranged by size:
4283 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 3657 3689 5023 5055 7974 8006 2063
Size: 32 1562 32 1334 32 2919 32 2071
Fragments arranged by size:
2919 2071 1562 1334 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8014
XbaI T'CTAG_A
Cuts at: 5350 5350
Size: 8014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3560 4761 3560
Size: 1201 6813
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8014
XmaI C'CCGG_G
Cuts at: 1870 2784 4404 5124 5339 1870
Size: 914 1620 720 215 4545
Fragments arranged by size:
4545 1620 914 720 215
XmnI GAAnn'nnTTC
Cuts at: 6143 6143
Size: 8014
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5822 276
Size: 53 83 186 936 4288 2468
Fragments arranged by size:
4288 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI BstZ17I
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SapI SrfI SwaI