(Circular) (Six-base) MAPSORT of: pFN21AA0619.seq Check: 7261 from: 1 to: 8869 pFN21AA0619 8869 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6679 278 Size: 53 83 186 936 5143 2468 Fragments arranged by size: 5143 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2257 3353 6142 6212 1937 Size: 320 1096 2789 70 4594 Fragments arranged by size: 4594 2789 1096 320 70 Acc65I G'GTAC_C Cuts at: 6190 6190 Size: 8869 AclI AA'CG_TT Cuts at: 1563 6996 7369 1563 Size: 5433 373 3063 Fragments arranged by size: 5433 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2613 2973 3512 3857 4004 4454 4821 5313 Size: 981 360 539 345 147 450 367 492 Cuts at: 5313 6932 8316 8584 1632 Size: 1619 1384 268 1917 Fragments arranged by size: 1917 1619 1384 981 539 492 450 367 360 345 268 147 AfeI AGC'GCT Cuts at: 4412 4412 Size: 8869 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8672 AflIII A'CryG_T Cuts at: 1203 2708 5853 7768 8745 1203 Size: 1505 3145 1915 977 1327 Fragments arranged by size: 3145 1915 1505 1327 977 AgeI A'CCGG_T Cuts at: 7700 7700 Size: 8869 AhdI GACnn_n'nnGTC Cuts at: 7598 7598 Size: 8869 AleI CACnn'nnGTG Cuts at: 1166 5168 1166 Size: 4002 4867 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3398 3555 1184 Size: 2214 157 6498 Fragments arranged by size: 6498 2214 157 AlwNI CAG_nnn'CTG Cuts at: 2405 3113 3728 3787 4775 5695 6612 8184 2405 Size: 708 615 59 988 920 917 1572 3090 Fragments arranged by size: 3090 1572 988 920 917 708 615 59 ApaLI G'TGCA_C Cuts at: 6926 8082 6926 Size: 1156 7713 ApoI r'AATT_y Cuts at: 1493 2155 2579 2935 2964 3259 3586 3860 5504 Size: 662 424 356 29 295 327 274 1644 Cuts at: 5504 5673 5732 5998 6178 6404 6556 8665 1493 Size: 169 59 266 180 226 152 2109 1697 Fragments arranged by size: 2109 1697 1644 662 424 356 327 295 274 266 226 180 169 152 59 29 AseI AT'TA_AT Cuts at: 160 1032 3927 7423 160 Size: 872 2895 3496 1606 Fragments arranged by size: 3496 2895 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8869 AvaI C'yCGr_G Cuts at: 1870 1943 2045 6194 1870 Size: 73 102 4149 4545 Fragments arranged by size: 4545 4149 102 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2739 2772 2739 Size: 33 8836 BamHI G'GATC_C Cuts at: 6199 6566 6199 Size: 367 8502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 6190 7645 618 Size: 325 217 627 252 4151 1455 1842 Fragments arranged by size: 4151 1842 1455 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 4338 4590 6188 1391 Size: 2947 252 1598 4072 Fragments arranged by size: 4072 2947 1598 252 BbeI G_GCGC'C Cuts at: 2043 2043 Size: 8869 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2280 928 Size: 971 381 7517 Fragments arranged by size: 7517 971 381 BbvCI CC'TCA_GC Cuts at: 5430 5430 Size: 8869 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2312 2346 3136 3170 4769 4803 7060 Size: 34 375 34 790 34 1599 34 2257 Cuts at: 7060 7094 1903 Size: 34 3678 Fragments arranged by size: 3678 2257 1599 790 375 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2869 6761 7971 2869 Size: 3892 1210 3767 Fragments arranged by size: 3892 3767 1210 BclI T'GATC_A Cuts at: 1548 3073 3562 5247 1548 Size: 1525 489 1685 5170 Fragments arranged by size: 5170 1685 1525 489 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4139 5848 7480 Size: 107 122 71 631 605 2467 1709 1632 Cuts at: 7480 136 Size: 1525 Fragments arranged by size: 2467 1709 1632 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3805 4164 8864 3805 Size: 359 4700 3810 Fragments arranged by size: 4700 3810 359 BlpI GC'TnA_GC Cuts at: 6286 6286 Size: 8869 Bme1580I G_kGCm'C Cuts at: 1790 6930 8086 1790 Size: 5140 1156 2573 Fragments arranged by size: 5140 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6600 7558 442 Size: 416 53 83 399 24 5183 958 1753 Fragments arranged by size: 5183 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 3668 3851 4025 4574 4769 4868 6051 Size: 875 1554 183 174 549 195 99 1183 Cuts at: 6051 7529 1239 Size: 1478 2579 Fragments arranged by size: 2579 1554 1478 1183 875 549 195 183 174 99 Bpu10I CC'TnA_GC Cuts at: 5430 5430 Size: 8869 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4937 5085 6994 7859 8157 4937 Size: 148 1909 865 298 5649 Fragments arranged by size: 5649 1909 865 298 148 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 2103 2523 7532 882 Size: 617 543 61 420 5009 2219 Fragments arranged by size: 5009 2219 617 543 420 61 BsaAI yAC'GTr Cuts at: 493 1204 2709 6660 493 Size: 711 1505 3951 2702 Fragments arranged by size: 3951 2702 1505 711 BsaBI GATnn'nnATC Cuts at: 2493 8863 2493 Size: 6370 2499 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 6676 7058 7693 Size: 53 83 186 936 507 4636 382 635 Cuts at: 7693 275 Size: 1451 Fragments arranged by size: 4636 1451 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2764 7302 7700 7974 8121 8532 2764 Size: 4538 398 274 147 411 3101 Fragments arranged by size: 4538 3101 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 2520 2550 3094 Size: 30 165 30 699 30 388 30 544 Cuts at: 3094 3124 1178 Size: 30 6923 Fragments arranged by size: 6923 699 544 388 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3476 5856 5945 1186 Size: 2290 2380 89 4110 Fragments arranged by size: 4110 2380 2290 89 BseYI C'CCAG_C Cuts at: 5238 6061 8072 8568 5238 Size: 823 2011 496 5539 Fragments arranged by size: 5539 2011 823 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 1163 Size: 573 606 7690 Fragments arranged by size: 7690 606 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6319 7080 8108 1540 Size: 275 186 4318 761 1028 2301 Fragments arranged by size: 4318 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4338 4590 6930 7015 8086 4338 Size: 252 2340 85 1071 5121 Fragments arranged by size: 5121 2340 1071 252 85 BsmI GAATG_Cn' Cuts at: 6380 6473 6380 Size: 93 8776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4338 4590 6188 6930 7015 8086 1391 Size: 399 2548 252 1598 742 85 1071 2174 Fragments arranged by size: 2548 2174 1598 1071 742 399 252 85 BspEI T'CCGG_A Cuts at: 8532 8532 Size: 8869 BspHI T'CATG_A Cuts at: 3565 4836 5127 6758 8488 3565 Size: 1271 291 1631 1730 3946 Fragments arranged by size: 3946 1730 1631 1271 291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6224 844 Size: 5380 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6756 1394 Size: 429 4933 3507 Fragments arranged by size: 4933 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3382 5477 7364 7538 65 Size: 1183 2134 2095 1887 174 1396 Fragments arranged by size: 2134 2095 1887 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2010 7513 7700 1700 Size: 110 200 5503 187 2869 Fragments arranged by size: 5503 2869 200 187 110 BsrGI T'GTAC_A Cuts at: 96 2499 96 Size: 2403 6466 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8869 BssSI C'ACGA_G Cuts at: 6929 7941 6929 Size: 1012 7857 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8869 BstBI TT'CG_AA Cuts at: 4549 5308 7673 4549 Size: 759 2365 5745 Fragments arranged by size: 5745 2365 759 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8869 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2131 Size: 8869 BstYI r'GATC_y Cuts at: 1972 3805 4164 6199 6566 6951 6968 8420 8506 Size: 1833 359 2035 367 385 17 1452 86 Cuts at: 8506 8518 8864 1972 Size: 12 346 1977 Fragments arranged by size: 2035 1977 1833 1452 385 367 359 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3354 3354 Size: 8869 Bsu36I CC'TnA_GG Cuts at: 2781 2781 Size: 8869 BtgI C'CryG_G Cuts at: 513 1065 2625 3910 513 Size: 552 1560 1285 5472 Fragments arranged by size: 5472 1560 1285 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2328 487 Size: 683 56 1102 7028 Fragments arranged by size: 7028 1102 683 56 BtsI GCAGTG_nn' Cuts at: 2586 3139 5419 6385 7179 7199 2586 Size: 553 2280 966 794 20 4256 Fragments arranged by size: 4256 2280 966 794 553 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8834 DraI TTT'AAA Cuts at: 3969 4242 6173 3969 Size: 273 1931 6665 Fragments arranged by size: 6665 1931 273 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6138 7876 809 Size: 507 4822 1738 1802 Fragments arranged by size: 4822 1802 1738 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6134 6316 7205 Size: 54 1425 50 275 18 4304 182 889 Cuts at: 7205 8834 8 Size: 1629 43 Fragments arranged by size: 4304 1629 1425 889 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6316 1537 Size: 275 4504 4090 Fragments arranged by size: 4504 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2296 3453 4004 5289 6003 6799 1353 Size: 943 1157 551 1285 714 796 3423 Fragments arranged by size: 3423 1285 1157 943 796 714 551 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7456 7830 7976 387 Size: 1064 138 5867 374 146 1280 Fragments arranged by size: 5867 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4336 4588 4336 Size: 252 8617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2613 2973 3512 3668 3851 3857 Size: 393 482 499 360 539 156 183 6 Cuts at: 3857 4004 4025 4454 4574 4769 4821 4868 5313 Size: 147 21 429 120 195 52 47 445 Cuts at: 5313 6051 6932 7529 8316 8584 1239 Size: 738 881 597 787 268 1524 Fragments arranged by size: 1524 881 787 738 597 539 499 482 445 429 393 360 268 195 183 156 147 120 52 47 21 6 EcoRI G'AATT_C Cuts at: 2935 6178 6556 2935 Size: 3243 378 5248 Fragments arranged by size: 5248 3243 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2325 2618 3386 3638 4240 5489 2325 Size: 293 768 252 602 1249 5705 Fragments arranged by size: 5705 1249 768 602 293 252 FspI TGC'GCA Cuts at: 7375 7375 Size: 8869 HaeII r_GCGC'y Cuts at: 1678 2043 2102 3524 4414 8016 1678 Size: 365 59 1422 890 3602 2531 Fragments arranged by size: 3602 2531 1422 890 365 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2759 2791 2822 Size: 32 135 32 530 32 625 32 31 Cuts at: 2822 2854 3266 3298 4997 5029 5116 5148 5201 Size: 32 412 32 1699 32 87 32 53 Cuts at: 5201 5233 5239 5271 7510 7542 7584 7616 1373 Size: 32 6 32 2239 32 42 32 2626 Fragments arranged by size: 2626 2239 1699 625 530 412 135 87 53 42 32 32 32 32 32 32 32 32 32 32 32 32 31 6 HincII GTy'rAC Cuts at: 669 1657 1938 3604 4456 6143 6213 6459 669 Size: 988 281 1666 852 1687 70 246 3079 Fragments arranged by size: 3079 1687 1666 988 852 281 246 70 HindIII A'AGCT_T Cuts at: 748 2366 3207 748 Size: 1618 841 6410 Fragments arranged by size: 6410 1618 841 HpaI GTT'AAC Cuts at: 3604 4456 6459 3604 Size: 852 2003 6014 Fragments arranged by size: 6014 2003 852 KasI G'GCGC_C Cuts at: 2039 2039 Size: 8869 KpnI G_GTAC'C Cuts at: 6194 6194 Size: 8869 MfeI C'AATT_G Cuts at: 4794 4905 6468 4794 Size: 111 1563 7195 Fragments arranged by size: 7195 1563 111 MluI A'CGCG_T Cuts at: 8745 8745 Size: 8869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2737 3196 5653 5731 7983 8167 1305 Size: 1432 459 2457 78 2252 184 2007 Fragments arranged by size: 2457 2252 2007 1432 459 184 78 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7047 Fragments arranged by size: 7047 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 2507 2643 2711 3537 4511 5168 Size: 648 963 378 136 68 826 974 657 Cuts at: 5168 6827 7186 7345 518 Size: 1659 359 159 2042 Fragments arranged by size: 2042 1659 974 963 826 657 648 378 359 159 136 68 MspA1I CmG'CkG Cuts at: 1606 3226 3787 5359 6285 6962 8110 8355 1606 Size: 1620 561 1572 926 677 1148 245 2120 Fragments arranged by size: 2120 1620 1572 1148 926 677 561 245 NaeI GCC'GGC Cuts at: 2012 2012 Size: 8869 NarI GG'CG_CC Cuts at: 2040 2040 Size: 8869 NcoI C'CATG_G Cuts at: 513 1065 2625 3910 513 Size: 552 1560 1285 5472 Fragments arranged by size: 5472 1560 1285 552 NdeI CA'TA_TG Cuts at: 387 5205 5916 387 Size: 4818 711 3340 Fragments arranged by size: 4818 3340 711 NgoMIV G'CCGG_C Cuts at: 2010 2010 Size: 8869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8869 NotI GC'GGCC_GC Cuts at: 6316 6316 Size: 8869 NspI r_CATG'y Cuts at: 1132 1229 2521 2665 2978 5857 6227 7772 1132 Size: 97 1292 144 313 2879 370 1545 2229 Fragments arranged by size: 2879 2229 1545 1292 370 313 144 97 PasI CC'CwG_GG Cuts at: 1348 2454 1348 Size: 1106 7763 PciI A'CATG_T Cuts at: 5853 7768 5853 Size: 1915 6954 PflMI CCAn_nnn'nTGG Cuts at: 1108 4507 1108 Size: 3399 5470 PfoI T'CCnGG_A Cuts at: 4590 4590 Size: 8869 PmeI GTTT'AAAC Cuts at: 6173 6173 Size: 8869 PmlI CAC'GTG Cuts at: 2709 2709 Size: 8869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6938 6970 8451 8483 6938 Size: 32 1481 32 7324 Fragments arranged by size: 7324 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8869 PsiI TTA'TAA Cuts at: 4632 6439 4632 Size: 1807 7062 PstI C_TGCA'G Cuts at: 830 3739 5322 6221 830 Size: 2909 1583 899 3478 Fragments arranged by size: 3478 2909 1583 899 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8869 PvuII CAG'CTG Cuts at: 3226 3787 5359 3226 Size: 561 1572 6736 Fragments arranged by size: 6736 1572 561 SacI G_AGCT'C Cuts at: 4338 4590 4338 Size: 252 8617 SalI G'TCGA_C Cuts at: 1936 6141 6211 1936 Size: 4205 70 4594 Fragments arranged by size: 4594 4205 70 SapI GCTCTTCn'nnn_ Cuts at: 6003 6003 Size: 8869 SbfI CC_TGCA'GG Cuts at: 6221 6221 Size: 8869 ScaI AGT'ACT Cuts at: 7117 8829 7117 Size: 1712 7157 SfcI C'TryA_G Cuts at: 826 1045 2258 2841 3114 3250 3735 5318 6003 Size: 219 1213 583 273 136 485 1583 685 Cuts at: 6003 6217 7352 8033 8224 826 Size: 214 1135 681 191 1471 Fragments arranged by size: 1583 1471 1213 1135 685 681 583 485 273 219 214 191 136 SfoI GGC'GCC Cuts at: 2041 2041 Size: 8869 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8869 SmaI CCC'GGG Cuts at: 1872 6196 1872 Size: 4324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4916 5100 6973 7874 8136 820 Size: 197 926 2973 184 1873 901 262 1553 Fragments arranged by size: 2973 1873 1553 926 901 262 197 184 SnaBI TAC'GTA Cuts at: 493 6660 493 Size: 6167 2702 SpeI A'CTAG_T Cuts at: 152 4940 152 Size: 4788 4081 SphI G_CATG'C Cuts at: 1132 6227 1132 Size: 5095 3774 SspI AAT'ATT Cuts at: 5 52 4811 6793 5 Size: 47 4759 1982 2081 Fragments arranged by size: 4759 2081 1982 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8869 StyI C'CwwG_G Cuts at: 513 1065 2160 2625 3910 5678 6308 513 Size: 552 1095 465 1285 1768 630 3074 Fragments arranged by size: 3074 1768 1285 1095 630 552 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2446 6896 6913 7066 7251 7696 325 Size: 1255 866 4450 17 153 185 445 1498 Fragments arranged by size: 4450 1498 1255 866 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2499 2514 7115 Size: 274 80 33 51 1443 522 15 4601 Cuts at: 7115 8827 96 Size: 1712 138 Fragments arranged by size: 4601 1712 1443 522 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2931 2963 3395 3427 8829 8861 2931 Size: 32 432 32 5402 32 2939 Fragments arranged by size: 5402 2939 432 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8869 XbaI T'CTAG_A Cuts at: 3883 6205 3883 Size: 2322 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3397 4207 5593 3397 Size: 810 1386 6673 Fragments arranged by size: 6673 1386 810 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8869 XmaI C'CCGG_G Cuts at: 1870 6194 1870 Size: 4324 4545 XmnI GAAnn'nnTTC Cuts at: 2598 6998 2598 Size: 4400 4469 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6677 276 Size: 53 83 186 936 5143 2468 Fragments arranged by size: 5143 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI DraIII EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI