Restriction map for
pFN21AA0591
##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
Acc65I AflII AflIII AgeI ApaI
AscI Asp718I AviII BbrPI BclI
BfrI BsiWI BsmBI BspLU11I BssHII
Bst1107I BstEII BstPI BstZ17I CpoI
DrdI EcoO65I FbaI FseI FspI
KpnI MluI NotI NruI NspI
PacI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SalI SbfI
ScaI SexAI SgrAI SpeI SphI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1362 GACGTC GACGT^C
AccII 999 CGCG CG^CG
AclI 1424 AACGTT AA^CGTT
AhdI 3133 GACTTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4798 GTGCAC G^TGCAC
AocI 3967 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1802 AGCGCT AGC^GCT
ApaLI 4798 GTGCAC G^TGCAC
AspEI 3133 GACTTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1566 CCTAGG C^CTAGG
BamHI 4851 GGATCC G^GATCC
BbeI 1694 GGCGCC GGCGC^C
BcgI 2703 CGAGCGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1566 CCTAGG C^CTAGG
BsaAI 1816 TACGTA [CT]AC^GT[GA]
BspDI 1192 ATCGAT AT^CGAT
BspHI 2442 TCATGA T^CATGA
BspMI 2148 GCAGGT ACCTGC(4/8)
BssSI 1919 CACGAG CACGAG(-5/-1)
BstBI 4302 TTCGAA TT^CGAA
BstUI 999 CGCG CG^CG
Bsu36I 3967 CCTGAGG CC^T[ACGT]AGG
ClaI 1192 ATCGAT AT^CGAT
DraI 1428 TTTAAA TTT^AAA
EagI 2744 CGGCCG C^GGCCG
Eam1105I 3133 GACTTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2744 CGGCCG C^GGCCG
Eco47III 1802 AGCGCT AGC^GCT
Eco52I 2744 CGGCCG C^GGCCG
Eco81I 3967 CCTGAGG CC^T[ACGT]AGG
EcoT22I 75 ATGCAT ATGCA^T
HpaI 1603 GTTAAC GTT^AAC
KasI 1694 GGCGCC G^GCGCC
KspI 998 CCGCGG CCGC^GG
MfeI 4841 CAATTG C^AATTG
MunI 4841 CAATTG C^AATTG
MvnI 999 CGCG CG^CG
NarI 1694 GGCGCC GG^CGCC
NdeI 4409 CATATG CA^TATG
NheI 1260 GCTAGC G^CTAGC
NsiI 75 ATGCAT ATGCA^T
NspV 4302 TTCGAA TT^CGAA
PaeR7I 50 CTCGAG C^TCGAG
Psp1406I 1424 AACGTT AA^CGTT
RcaI 2442 TCATGA T^CATGA
SacII 998 CCGCGG CCGC^GG
SapI 1732 GAAGAGC GCTCTTC(1/4)
SfiI 2238 GGCCCAATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1694 GGCGCC GGC^GCC
SfuI 4302 TTCGAA TT^CGAA
SmaI 4964 CCCGGG CCC^GGG
SnaBI 1816 TACGTA TAC^GTA
XhoI 50 CTCGAG C^TCGAG
XmaCI 4964 CCCGGG C^CCGGG
XmaI 4964 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1395 GTCTAC GT^[AC][GT]AC
4915 GTAGAC
AccIII 1748 TCCGGA T^CCGGA
2252 TCCGGA
AseI 811 ATTAAT AT^TAAT
3247 ATTAAT
AsnI 811 ATTAAT AT^TAAT
3247 ATTAAT
AspI 3197 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4149 GACCTTGTC
BanI 775 GGTGCC G^G[CT][GA]CC
1694 GGCGCC
BciVI 5116 GGATAC GTATCC(6/5)
2952 GTATCC
BsaBI 1177 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4300 GATTCGAATC
BsaWI 1748 TCCGGA [AT]^CCGG[AT]
2252 TCCGGA
BseAI 1748 TCCGGA T^CCGGA
2252 TCCGGA
BsmI 2812 GCATTC GAATGC(1/-1)
3445 GCATTC
Bsp1407I 218 TGTACA T^GTACA
3027 TGTACA
BspEI 1748 TCCGGA T^CCGGA
2252 TCCGGA
BsrBI 1690 GAGCGG CCGCTC(-3/-3)
1713 GAGCGG
BsrFI 2827 GCCGGC [GA]^CCGG[CT]
4188 GCCGGC
BsrGI 218 TGTACA T^GTACA
3027 TGTACA
Cfr10I 2827 GCCGGC [GA]^CCGG[CT]
4188 GCCGGC
DraIII 652 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2971 CACAGGGTG
EcoRI 3074 GAATTC G^AATTC
4786 GAATTC
HincII 1603 GTTAAC GT[CT]^[GA]AC
3814 GTTGAC
HindII 1603 GTTAAC GT[CT]^[GA]AC
3814 GTTGAC
HindIII 1480 AAGCTT A^AGCTT
5413 AAGCTT
MamI 1177 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4300 GATTCGAATC
MroI 1748 TCCGGA T^CCGGA
2252 TCCGGA
NaeI 2827 GCCGGC GCC^GGC
4188 GCCGGC
NcoI 1304 CCATGG C^CATGG
5279 CCATGG
NgoMIV 2827 GCCGGC G^CCGGC
4188 GCCGGC
PflFI 3197 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4149 GACCTTGTC
PflMI 514 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2970 CCACAGGGTGG
PshBI 811 ATTAAT AT^TAAT
3247 ATTAAT
PstI 2171 CTGCAG CTGCA^G
3983 CTGCAG
SspBI 218 TGTACA T^GTACA
3027 TGTACA
StuI 2135 AGGCCT AGG^CCT
2452 AGGCCT
Tth111I 3197 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4149 GACCTTGTC
Van91I 514 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2970 CCACAGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI