Restriction map for
pFN21AA0591
(Linear) (Six-base) MAPSORT of: KIAA0591.seq Check: 7438 from: 1 to: 5448
KIAA0591.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AatII G_ACGT'C
Cuts at: 0 1366 5448
Size: 1366 4082
AccI GT'mk_AC
Cuts at: 0 1396 4916 5448
Size: 1396 3520 532
Fragments arranged by size:
3520 1396 532
AclI AA'CG_TT
Cuts at: 0 1425 5448
Size: 1425 4023
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1156 2204 2850 3332 4051 5448
Size: 132 1024 1048 646 482 719 1397
Fragments arranged by size:
1397 1048 1024 719 646 482 132
AfeI AGC'GCT
Cuts at: 0 1804 5448
Size: 1804 3644
AhdI GACnn_n'nnGTC
Cuts at: 0 3138 5448
Size: 3138 2310
AleI CACnn'nnGTG
Cuts at: 0 2693 5448
Size: 2693 2755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 314 3530 5448
Size: 256 58 3216 1918
Fragments arranged by size:
3216 1918 256 58
AlwNI CAG_nnn'CTG
Cuts at: 0 1161 1687 2112 2544 4817 5150 5448
Size: 1161 526 425 432 2273 333 298
Fragments arranged by size:
2273 1161 526 432 425 333 298
ApaLI G'TGCA_C
Cuts at: 0 4798 5448
Size: 4798 650
ApoI r'AATT_y
Cuts at: 0 443 951 2227 3074 4786 4987 5053 5448
Size: 443 508 1276 847 1712 201 66 395
Fragments arranged by size:
1712 1276 847 508 443 395 201 66
AseI AT'TA_AT
Cuts at: 0 812 3248 5448
Size: 812 2436 2200
Fragments arranged by size:
2436 2200 812
AvaI C'yCGr_G
Cuts at: 0 50 4229 4964 5036 5430 5448
Size: 50 4179 735 72 394 18
Fragments arranged by size:
4179 735 394 72 50 18
AvrII C'CTAG_G
Cuts at: 0 1566 5448
Size: 1566 3882
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4388 4421 5448
Size: 4388 33 1027
Fragments arranged by size:
4388 1027 33
BamHI G'GATC_C
Cuts at: 0 4851 5448
Size: 4851 597
BanI G'GyrC_C
Cuts at: 0 775 1694 5448
Size: 775 919 3754
Fragments arranged by size:
3754 919 775
BanII G_rGCy'C
Cuts at: 0 997 1722 1796 2677 3461 3917 4502 5448
Size: 997 725 74 881 784 456 585 946
Fragments arranged by size:
997 946 881 784 725 585 456 74
BbeI G_GCGC'C
Cuts at: 0 1698 5448
Size: 1698 3750
BbsI GAAGACnn'nnnn_
Cuts at: 0 1622 1942 4028 4212 4951 5181 5293 5448
Size: 1622 320 2086 184 739 230 112 155
Fragments arranged by size:
2086 1622 739 320 230 184 155 112
BbvCI CC'TCA_GC
Cuts at: 0 3638 4600 4615 5448
Size: 3638 962 15 833
Fragments arranged by size:
3638 962 833 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2692 2726 5448
Size: 2692 34 2722
Fragments arranged by size:
2722 2692 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2963 5110 5448
Size: 2963 2147 338
Fragments arranged by size:
2963 2147 338
BfrBI ATG'CAT
Cuts at: 0 77 5448
Size: 77 5371
BglI GCCn_nnn'nGGC
Cuts at: 0 347 2245 4274 5448
Size: 347 1898 2029 1174
Fragments arranged by size:
2029 1898 1174 347
BglII A'GATC_T
Cuts at: 0 2057 2088 2859 4139 4241 4643 5448
Size: 2057 31 771 1280 102 402 805
Fragments arranged by size:
2057 1280 805 771 402 102 31
BlpI GC'TnA_GC
Cuts at: 0 1688 1711 1925 5448
Size: 1688 23 214 3523
Fragments arranged by size:
3523 1688 214 23
Bme1580I G_kGCm'C
Cuts at: 0 2598 2963 4802 5448
Size: 2598 365 1839 646
Fragments arranged by size:
2598 1839 646 365
BmrI ACTGGGnnnn_n'
Cuts at: 0 3346 5160 5448
Size: 3346 1814 288
Fragments arranged by size:
3346 1814 288
BmtI G_CTAG'C
Cuts at: 0 1264 5448
Size: 1264 4184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1736 1974 2604 4477 4949 5448
Size: 1736 238 630 1873 472 499
Fragments arranged by size:
1873 1736 630 499 472 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1424 2574 3212 3802 4507 4516 5448
Size: 1424 1150 638 590 705 9 932
Fragments arranged by size:
1424 1150 932 705 638 590 9
Bpu10I CC'TnA_GC
Cuts at: 0 1154 1857 2938 3638 4576 4600 4615 5448
Size: 1154 703 1081 700 938 24 15 833
Fragments arranged by size:
1154 1081 938 833 703 700 24 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2518 4489 5448
Size: 2518 1971 959
Fragments arranged by size:
2518 1971 959
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 176 691 1945 5109 5448
Size: 49 127 515 1254 3164 339
Fragments arranged by size:
3164 1254 515 339 127 49
BsaAI yAC'GTr
Cuts at: 0 1818 5448
Size: 1818 3630
BsaBI GATnn'nnATC
Cuts at: 0 1181 4304 5448
Size: 1181 3123 1144
Fragments arranged by size:
3123 1181 1144
BsaHI Gr'CG_yC
Cuts at: 0 1363 1695 2825 5448
Size: 1363 332 1130 2623
Fragments arranged by size:
2623 1363 1130 332
BsaWI w'CCGG_w
Cuts at: 0 1748 2252 5448
Size: 1748 504 3196
Fragments arranged by size:
3196 1748 504
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 526 556 4872 4902 5448
Size: 29 497 30 4316 30 546
Fragments arranged by size:
4316 546 497 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1135 1421 2203 2863 3190 3215 4626 4884
Size: 1135 286 782 660 327 25 1411 258
Cuts at: 4884 5448
Size: 564
Fragments arranged by size:
1411 1135 782 660 564 327 286 258
25
BseYI C'CCAG_C
Cuts at: 0 592 1838 3804 4594 5010 5448
Size: 592 1246 1966 790 416 438
Fragments arranged by size:
1966 1246 790 592 438 416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 2380 3526 5295 5448
Size: 855 1525 1146 1769 153
Fragments arranged by size:
1769 1525 1146 855 153
BsiEI CG_ry'CG
Cuts at: 0 2718 2747 2895 5448
Size: 2718 29 148 2553
Fragments arranged by size:
2718 2553 148 29
BsiHKAI G_wGCw'C
Cuts at: 0 1030 1155 2677 3917 4475 4502 4802 5448
Size: 1030 125 1522 1240 558 27 300 646
Fragments arranged by size:
1522 1240 1030 646 558 300 125 27
BsmI GAATG_Cn'
Cuts at: 0 2812 3445 5448
Size: 2812 633 2003
Fragments arranged by size:
2812 2003 633
Bsp1286I G_dGCh'C
Cuts at: 0 997 1030 1155 1722 1796 2598 2677 2963
Size: 997 33 125 567 74 802 79 286
Cuts at: 2963 3461 3917 4475 4502 4802 5448
Size: 498 456 558 27 300 646
Fragments arranged by size:
997 802 646 567 558 498 456 300
286 125 79 74 33 27
BspEI T'CCGG_A
Cuts at: 0 1748 2252 5448
Size: 1748 504 3196
Fragments arranged by size:
3196 1748 504
BspHI T'CATG_A
Cuts at: 0 2442 5448
Size: 2442 3006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2139 5448
Size: 2139 3309
BsrBI CCG'CTC
Cuts at: 0 1692 1715 5448
Size: 1692 23 3733
Fragments arranged by size:
3733 1692 23
BsrDI GCAATG_nn'
Cuts at: 0 572 983 1074 1448 2310 3155 4176 5448
Size: 572 411 91 374 862 845 1021 1272
Fragments arranged by size:
1272 1021 862 845 572 411 374 91
BsrFI r'CCGG_y
Cuts at: 0 2827 4188 5448
Size: 2827 1361 1260
Fragments arranged by size:
2827 1361 1260
BsrGI T'GTAC_A
Cuts at: 0 218 3027 5448
Size: 218 2809 2421
Fragments arranged by size:
2809 2421 218
BssSI C'ACGA_G
Cuts at: 0 1919 5448
Size: 1919 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 0 982 1048 2139 3991 5448
Size: 982 66 1091 1852 1457
Fragments arranged by size:
1852 1457 1091 982 66
BstBI TT'CG_AA
Cuts at: 0 4303 5448
Size: 4303 1145
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 1506 2340 2430 5448
Size: 612 894 834 90 3018
Fragments arranged by size:
3018 894 834 612 90
BstYI r'GATC_y
Cuts at: 0 189 537 741 2057 2088 2859 4139 4241
Size: 189 348 204 1316 31 771 1280 102
Cuts at: 4241 4643 4851 5448
Size: 402 208 597
Fragments arranged by size:
1316 1280 771 597 402 348 208 204
189 102 31
Bsu36I CC'TnA_GG
Cuts at: 0 3968 5448
Size: 3968 1480
BtgI C'CryG_G
Cuts at: 0 998 1304 2688 2741 3452 4449 5279 5322
Size: 998 306 1384 53 711 997 830 43
Cuts at: 5322 5448
Size: 126
Fragments arranged by size:
1384 998 997 830 711 306 126 53
43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3021 3415 5448
Size: 3021 394 2033
Fragments arranged by size:
3021 2033 394
BtsI GCAGTG_nn'
Cuts at: 0 3348 3524 4611 4695 4881 5448
Size: 3348 176 1087 84 186 567
Fragments arranged by size:
3348 1087 567 186 176 84
ClaI AT'CG_AT
Cuts at: 0 1193 5448
Size: 1193 4255
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4641 4676 5081 5116 5448
Size: 4641 35 405 35 332
Fragments arranged by size:
4641 405 332 35 35
DraI TTT'AAA
Cuts at: 0 1430 5448
Size: 1430 4018
DraIII CAC_nnn'GTG
Cuts at: 0 657 2976 5448
Size: 657 2319 2472
Fragments arranged by size:
2472 2319 657
EaeI y'GGCC_r
Cuts at: 0 854 1284 1674 2744 2978 3729 5448
Size: 854 430 390 1070 234 751 1719
Fragments arranged by size:
1719 1070 854 751 430 390 234
EagI C'GGCC_G
Cuts at: 0 2744 5448
Size: 2744 2704
EarI CTCTTCn'nnn_
Cuts at: 0 1469 1727 2180 2192 2869 3308 4418 5448
Size: 1469 258 453 12 677 439 1110 1030
Fragments arranged by size:
1469 1110 1030 677 453 439 258 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3623 5448
Size: 3623 1825
EcoICRI GAG'CTC
Cuts at: 0 2675 3915 4500 5448
Size: 2675 1240 585 948
Fragments arranged by size:
2675 1240 948 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1156 1424 2204 2574 2850 3212 3332
Size: 132 1024 268 780 370 276 362 120
Cuts at: 3332 3802 4051 4507 4516 5448
Size: 470 249 456 9 932
Fragments arranged by size:
1024 932 780 470 456 370 362 276
268 249 132 120 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2320 4507 5335 5448
Size: 2320 2187 828 113
Fragments arranged by size:
2320 2187 828 113
EcoO109I rG'GnC_Cy
Cuts at: 0 38 1086 1140 1158 1310 4055 4610 5328
Size: 38 1048 54 18 152 2745 555 718
Cuts at: 5328 5340 5448
Size: 12 108
Fragments arranged by size:
2745 1048 718 555 152 108 54 38
18 12
EcoRI G'AATT_C
Cuts at: 0 3074 4786 5448
Size: 3074 1712 662
Fragments arranged by size:
3074 1712 662
EcoRV GAT'ATC
Cuts at: 0 1005 3405 4833 5448
Size: 1005 2400 1428 615
Fragments arranged by size:
2400 1428 1005 615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1498 2107 5137 5448
Size: 1498 609 3030 311
Fragments arranged by size:
3030 1498 609 311
HaeII r_GCGC'y
Cuts at: 0 1698 1806 2709 4670 5448
Size: 1698 108 903 1961 778
Fragments arranged by size:
1961 1698 903 778 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 254 286 526 558 1186 1218 1581 1613
Size: 254 32 240 32 628 32 363 32
Cuts at: 1613 2815 2839 2846 2847 2871 2878 3052 3084
Size: 1202 24 7 1 24 7 174 32
Cuts at: 3084 3124 3156 3250 3282 3785 3817 4124 4156
Size: 40 32 94 32 503 32 307 32
Cuts at: 4156 4573 4605 5448
Size: 417 32 843
Fragments arranged by size:
1202 843 628 503 417 363 307 254
240 174 94 40 32 32 32 32
32 32 32 32 32 32 24 24
7 7 1
HincII GTy'rAC
Cuts at: 0 1605 3816 5448
Size: 1605 2211 1632
Fragments arranged by size:
2211 1632 1605
HindIII A'AGCT_T
Cuts at: 0 1480 5413 5448
Size: 1480 3933 35
Fragments arranged by size:
3933 1480 35
HpaI GTT'AAC
Cuts at: 0 1605 5448
Size: 1605 3843
KasI G'GCGC_C
Cuts at: 0 1694 5448
Size: 1694 3754
MfeI C'AATT_G
Cuts at: 0 4841 5448
Size: 4841 607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2777 5448
Size: 2777 2671
MscI TGG'CCA
Cuts at: 0 1286 1676 2980 3731 5448
Size: 1286 390 1304 751 1717
Fragments arranged by size:
1717 1304 1286 751 390
MslI CAynn'nnrTG
Cuts at: 0 85 363 692 1504 2338 2428 2693 2914
Size: 85 278 329 812 834 90 265 221
Cuts at: 2914 3434 5448
Size: 520 2014
Fragments arranged by size:
2014 834 812 520 329 278 265 221
90 85
MspA1I CmG'CkG
Cuts at: 0 1000 1769 1851 3043 3808 4280 5014 5448
Size: 1000 769 82 1192 765 472 734 434
Fragments arranged by size:
1192 1000 769 765 734 472 434 82
NaeI GCC'GGC
Cuts at: 0 2829 4190 5448
Size: 2829 1361 1258
Fragments arranged by size:
2829 1361 1258
NarI GG'CG_CC
Cuts at: 0 1695 5448
Size: 1695 3753
NcoI C'CATG_G
Cuts at: 0 1304 5279 5448
Size: 1304 3975 169
Fragments arranged by size:
3975 1304 169
NdeI CA'TA_TG
Cuts at: 0 4410 5448
Size: 4410 1038
NgoMIV G'CCGG_C
Cuts at: 0 2827 4188 5448
Size: 2827 1361 1260
Fragments arranged by size:
2827 1361 1260
NheI G'CTAG_C
Cuts at: 0 1260 5448
Size: 1260 4188
NsiI A_TGCA'T
Cuts at: 0 79 5448
Size: 79 5369
PflMI CCAn_nnn'nTGG
Cuts at: 0 520 2976 5448
Size: 520 2456 2472
Fragments arranged by size:
2472 2456 520
PfoI T'CCnGG_A
Cuts at: 0 2486 5448
Size: 2486 2962
PpuMI rG'GwC_Cy
Cuts at: 0 1086 1140 1310 4055 4610 5328 5448
Size: 1086 54 170 2745 555 718 120
Fragments arranged by size:
2745 1086 718 555 170 120 54
PstI C_TGCA'G
Cuts at: 0 2175 3987 5448
Size: 2175 1812 1461
Fragments arranged by size:
2175 1812 1461
PvuII CAG'CTG
Cuts at: 0 1851 3808 5014 5448
Size: 1851 1957 1206 434
Fragments arranged by size:
1957 1851 1206 434
SacI G_AGCT'C
Cuts at: 0 2677 3917 4502 5448
Size: 2677 1240 585 946
Fragments arranged by size:
2677 1240 946 585
SacII CC_GC'GG
Cuts at: 0 1001 5448
Size: 1001 4447
SanDI GG'GwC_CC
Cuts at: 0 1310 4610 5448
Size: 1310 3300 838
Fragments arranged by size:
3300 1310 838
SapI GCTCTTCn'nnn_
Cuts at: 0 1727 5448
Size: 1727 3721
SfcI C'TryA_G
Cuts at: 0 422 915 2044 2171 3788 3983 5448
Size: 422 493 1129 127 1617 195 1465
Fragments arranged by size:
1617 1465 1129 493 422 195 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2245 5448
Size: 2245 3203
SfoI GGC'GCC
Cuts at: 0 1696 5448
Size: 1696 3752
SmaI CCC'GGG
Cuts at: 0 4966 5448
Size: 4966 482
SmlI C'TyrA_G
Cuts at: 0 50 2533 4468 5448
Size: 50 2483 1935 980
Fragments arranged by size:
2483 1935 980 50
SnaBI TAC'GTA
Cuts at: 0 1818 5448
Size: 1818 3630
SspI AAT'ATT
Cuts at: 0 810 3513 4011 5448
Size: 810 2703 498 1437
Fragments arranged by size:
2703 1437 810 498
StuI AGG'CCT
Cuts at: 0 2137 2454 5448
Size: 2137 317 2994
Fragments arranged by size:
2994 2137 317
StyI C'CwwG_G
Cuts at: 0 851 1304 1566 2212 2902 3170 3726 4202
Size: 851 453 262 646 690 268 556 476
Cuts at: 4202 5279 5448
Size: 1077 169
Fragments arranged by size:
1077 851 690 646 556 476 453 268
262 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 527 691 2909 3614 3681 4742 4783 5448
Size: 527 164 2218 705 67 1061 41 665
Fragments arranged by size:
2218 1061 705 665 527 164 67 41
TatI w'GTAC_w
Cuts at: 0 218 927 2393 3027 5258 5448
Size: 218 709 1466 634 2231 190
Fragments arranged by size:
2231 1466 709 634 218 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 515 547 4344 4376 4872 4904 5448
Size: 31 484 32 3797 32 496 32 544
Fragments arranged by size:
3797 544 496 484 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 3200 4152 5448
Size: 3200 952 1296
Fragments arranged by size:
3200 1296 952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1098 1302 5250 5448
Size: 1098 204 3948 198
Fragments arranged by size:
3948 1098 204 198
XhoI C'TCGA_G
Cuts at: 0 50 5448
Size: 50 5398
XmaI C'CCGG_G
Cuts at: 0 4964 5448
Size: 4964 484
XmnI GAAnn'nnTTC
Cuts at: 0 2207 2417 4997 5448
Size: 2207 210 2580 451
Fragments arranged by size:
2580 2207 451 210
ZraI GAC'GTC
Cuts at: 0 1364 5448
Size: 1364 4084
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AhdI AleI AlfI
AlwNI ApaLI ApoI AseI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI KasI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
PflMI PfoI PpuMI PstI PvuII SacI SacII SanDI
SapI SfcI SfiI SfoI SmaI SmlI SnaBI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AflII AflIII AgeI AloI ApaI AscI
AsiSI BclI BmgBI BsiWI BsmBI BssHII BstEII BstZ17I
DrdI FseI FspI FspAI KpnI MluI NotI NruI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SbfI
ScaI SexAI SgfI SgrAI SpeI SphI SrfI SwaI
XbaI