Restriction map for
pFN21AA0577
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI AspEI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI BspEI BspLU11I BssHII BstAPI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoT22I FspI
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tsp509I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 2211 GTATAC GT^[AC][GT]AC
AccII 995 CGCG CG^CG
AflIII 2769 ACGTGT A^C[GA][CT]GT
AocI 381 CCTCAGG CC^T[ACGT]AGG
AspI 2575 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2150 CCTCAGC CCTCAGC(-5/-2)
BclI 1604 TGATCA T^GATCA
BsaWI 653 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2706 TGTACA T^GTACA
BspDI 2718 ATCGAT AT^CGAT
BspHI 2987 TCATGA T^CATGA
BsrGI 2706 TGTACA T^GTACA
Bst1107I 2211 GTATAC GTA^TAC
BstBI 1709 TTCGAA TT^CGAA
BstEII 2626 GGTGACC G^GT[ACGT]ACC
BstPI 2626 GGTGACC G^GT[ACGT]ACC
BstUI 995 CGCG CG^CG
BstZ17I 2211 GTATAC GTA^TAC
Bsu36I 381 CCTCAGG CC^T[ACGT]AGG
ClaI 2718 ATCGAT AT^CGAT
DrdI 2575 GACAAGGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 662 CCATGG C^C[GA][CT]GG
Eco81I 381 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2626 GGTGACC G^GT[ACGT]ACC
EcoRV 1828 GATATC GAT^ATC
FbaI 1604 TGATCA T^GATCA
FseI 170 GGCCGGCC GGCCGG^CC
MvnI 995 CGCG CG^CG
NcoI 662 CCATGG C^CATGG
NdeI 2354 CATATG CA^TATG
NspV 1709 TTCGAA TT^CGAA
PflFI 2575 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2987 TCATGA T^CATGA
SexAI 2633 ACCTGGT A^CC[AT]GGT
SfuI 1709 TTCGAA TT^CGAA
SgrAI 8 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2706 TGTACA T^GTACA
StuI 2147 AGGCCT AGG^CCT
Tth111I 2575 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1052 CCAAGTATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Acc65I 94 GGTACC G^GTACC
207 GGTACC
AcyI 6 GACGCC G[GA]^CG[CT]C
1696 GACGCC
Aor51HI 23 AGCGCT AGC^GCT
104 AGCGCT
ApaI 263 GGGCCC GGGCC^C
1025 GGGCCC
Asp700I 508 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2196 GAAGTGCTTC
Asp718I 94 GGTACC G^GTACC
207 GGTACC
BamHI 2070 GGATCC G^GATCC
2312 GGATCC
BanI 94 GGTACC G^G[CT][GA]CC
207 GGTACC
BsaHI 6 GACGCC G[GA]^CG[CT]C
1696 GACGCC
BsaI 1081 GAGACC GGTCTC(1/5)
2782 GGTCTC
BspMI 1724 GCAGGT ACCTGC(4/8)
2176 GCAGGT
BsrDI 2025 CATTGC GCAATG(2/0)
2282 GCAATG
BstXI 1373 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1815 CCAGCCCCCTGG
DraIII 46 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2655 CACACAGTG
EaeI 641 TGGCCG [CT]^GGCC[GA]
1382 TGGCCG
Eco47III 23 AGCGCT AGC^GCT
104 AGCGCT
Hin1I 6 GACGCC G[GA]^CG[CT]C
1696 GACGCC
KpnI 94 GGTACC GGTAC^C
207 GGTACC
NspI 896 ACATGC [GA]CATG^[CT]
1961 ACATGC
PstI 2139 CTGCAG CTGCA^G
2189 CTGCAG
SapI 2091 GAAGAGC GCTCTTC(1/4)
3112 GAAGAGC
XmnI 508 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2196 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI