Restriction map for
pFN21AA0577
(Linear) (Six-base) MAPSORT of: KIAA0577.seq Check: 6704 from: 1 to: 3123
KIAA0577.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 2212 3123
Size: 2212 911
Acc65I G'GTAC_C
Cuts at: 0 94 207 3123
Size: 94 113 2916
Fragments arranged by size:
2916 113 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1036 1637 3123
Size: 499 537 601 1486
Fragments arranged by size:
1486 601 537 499
AfeI AGC'GCT
Cuts at: 0 25 106 3123
Size: 25 81 3017
Fragments arranged by size:
3017 81 25
AflIII A'CryG_T
Cuts at: 0 2769 3123
Size: 2769 354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 123 2214 2405 3123
Size: 123 2091 191 718
Fragments arranged by size:
2091 718 191 123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2062 2094 3123
Size: 2062 32 1029
Fragments arranged by size:
2062 1029 32
AlwNI CAG_nnn'CTG
Cuts at: 0 87 522 1765 2257 2530 2792 3000 3123
Size: 87 435 1243 492 273 262 208 123
Fragments arranged by size:
1243 492 435 273 262 208 123 87
ApaI G_GGCC'C
Cuts at: 0 267 1029 3123
Size: 267 762 2094
Fragments arranged by size:
2094 762 267
AvaI C'yCGr_G
Cuts at: 0 176 239 260 839 911 926 1028 1388
Size: 176 63 21 579 72 15 102 360
Cuts at: 1388 1397 2732 3123
Size: 9 1335 391
Fragments arranged by size:
1335 579 391 360 176 102 72 63
21 15 9
BamHI G'GATC_C
Cuts at: 0 2070 2312 3123
Size: 2070 242 811
Fragments arranged by size:
2070 811 242
BanI G'GyrC_C
Cuts at: 0 94 207 3123
Size: 94 113 2916
Fragments arranged by size:
2916 113 94
BanII G_rGCy'C
Cuts at: 0 267 809 963 1029 1052 1223 1637 1909
Size: 267 542 154 66 23 171 414 272
Cuts at: 1909 1925 2403 2423 2733 2762 3123
Size: 16 478 20 310 29 361
Fragments arranged by size:
542 478 414 361 310 272 267 171
154 66 29 23 20 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 656 1288 2895 3123
Size: 308 348 632 1607 228
Fragments arranged by size:
1607 632 348 308 228
BbvCI CC'TCA_GC
Cuts at: 0 2151 3123
Size: 2151 972
BclI T'GATC_A
Cuts at: 0 1604 3123
Size: 1604 1519
BglI GCCn_nnn'nGGC
Cuts at: 0 1393 1904 2946 3123
Size: 1393 511 1042 177
Fragments arranged by size:
1393 1042 511 177
BlpI GC'TnA_GC
Cuts at: 0 61 545 707 784 3123
Size: 61 484 162 77 2339
Fragments arranged by size:
2339 484 162 77 61
Bme1580I G_kGCm'C
Cuts at: 0 267 1029 2001 3123
Size: 267 762 972 1122
Fragments arranged by size:
1122 972 762 267
BmgBI CAC'GTC
Cuts at: 0 75 777 2429 3123
Size: 75 702 1652 694
Fragments arranged by size:
1652 702 694 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 2679 3123
Size: 75 2604 444
Fragments arranged by size:
2604 444 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 1237 1290 1533 3123
Size: 63 1174 53 243 1590
Fragments arranged by size:
1590 1174 243 63 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 292 337 544 757 895 1090 1096
Size: 40 252 45 207 213 138 195 6
Cuts at: 1096 1742 1867 3022 3046 3123
Size: 646 125 1155 24 77
Fragments arranged by size:
1155 646 252 213 207 195 138 125
77 45 40 24 6
Bpu10I CC'TnA_GC
Cuts at: 0 671 1275 1631 2151 2307 3123
Size: 671 604 356 520 156 816
Fragments arranged by size:
816 671 604 520 356 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 748 1621 2260 2284 3123
Size: 619 129 873 639 24 839
Fragments arranged by size:
873 839 639 619 129 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1075 2788 3123
Size: 1075 1713 335
Fragments arranged by size:
1713 1075 335
BsaHI Gr'CG_yC
Cuts at: 0 7 1697 3123
Size: 7 1690 1426
Fragments arranged by size:
1690 1426 7
BsaWI w'CCGG_w
Cuts at: 0 653 3123
Size: 653 2470
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1039 1069 1740 1770 2371 2401 3123
Size: 1039 30 671 30 601 30 722
Fragments arranged by size:
1039 722 671 601 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 337 421 541 772 961 985 1003
Size: 136 201 84 120 231 189 24 18
Cuts at: 1003 1087 1090 1093 1138 1231 1327 1870 2527
Size: 84 3 3 45 93 96 543 657
Cuts at: 2527 2910 3123
Size: 383 213
Fragments arranged by size:
657 543 383 231 213 201 189 136
120 96 93 84 84 45 24 18
3 3
BseYI C'CCAG_C
Cuts at: 0 26 856 983 1157 1814 3123
Size: 26 830 127 174 657 1309
Fragments arranged by size:
1309 830 657 174 127 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 93 151 1332 1440 3123
Size: 28 65 58 1181 108 1683
Fragments arranged by size:
1683 1181 108 65 58 28
BsiHKAI G_wGCw'C
Cuts at: 0 809 1223 1637 1925 2423 3123
Size: 809 414 414 288 498 700
Fragments arranged by size:
809 700 498 414 414 288
Bsp1286I G_dGCh'C
Cuts at: 0 267 809 963 1029 1052 1223 1637 1909
Size: 267 542 154 66 23 171 414 272
Cuts at: 1909 1925 2001 2403 2423 2733 2762 3123
Size: 16 76 402 20 310 29 361
Fragments arranged by size:
542 414 402 361 310 272 267 171
154 76 66 29 23 20 16
BspHI T'CATG_A
Cuts at: 0 2987 3123
Size: 2987 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1715 2167 3123
Size: 1715 452 956
Fragments arranged by size:
1715 956 452
BsrBI CCG'CTC
Cuts at: 0 69 549 798 3123
Size: 69 480 249 2325
Fragments arranged by size:
2325 480 249 69
BsrDI GCAATG_nn'
Cuts at: 0 2024 2289 3123
Size: 2024 265 834
Fragments arranged by size:
2024 834 265
BsrFI r'CCGG_y
Cuts at: 0 9 171 788 2454 3123
Size: 9 162 617 1666 669
Fragments arranged by size:
1666 669 617 162 9
BsrGI T'GTAC_A
Cuts at: 0 2706 3123
Size: 2706 417
BssSI C'ACGA_G
Cuts at: 0 793 2233 3107 3123
Size: 793 1440 874 16
Fragments arranged by size:
1440 874 793 16
BstBI TT'CG_AA
Cuts at: 0 1710 3123
Size: 1710 1413
BstEII G'GTnAC_C
Cuts at: 0 2626 3123
Size: 2626 497
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1380 1822 3123
Size: 1380 442 1301
Fragments arranged by size:
1380 1301 442
BstYI r'GATC_y
Cuts at: 0 159 831 936 1292 1802 2070 2261 2312
Size: 159 672 105 356 510 268 191 51
Cuts at: 2312 2516 3066 3123
Size: 204 550 57
Fragments arranged by size:
672 550 510 356 268 204 191 159
105 57 51
BstZ17I GTA'TAC
Cuts at: 0 2213 3123
Size: 2213 910
Bsu36I CC'TnA_GG
Cuts at: 0 382 3123
Size: 382 2741
BtgI C'CryG_G
Cuts at: 0 662 3123
Size: 662 2461
BtsI GCAGTG_nn'
Cuts at: 0 227 324 1667 2362 2828 3123
Size: 227 97 1343 695 466 295
Fragments arranged by size:
1343 695 466 295 227 97
ClaI AT'CG_AT
Cuts at: 0 2719 3123
Size: 2719 404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1671 1706 3123
Size: 1671 35 1417
Fragments arranged by size:
1671 1417 35
DraIII CAC_nnn'GTG
Cuts at: 0 51 2660 3123
Size: 51 2609 463
Fragments arranged by size:
2609 463 51
DrdI GACnn_nn'nnGTC
Cuts at: 0 2581 3123
Size: 2581 542
EaeI y'GGCC_r
Cuts at: 0 641 1382 3123
Size: 641 741 1741
Fragments arranged by size:
1741 741 641
EarI CTCTTCn'nnn_
Cuts at: 0 305 398 407 611 2086 2685 3107 3123
Size: 305 93 9 204 1475 599 422 16
Fragments arranged by size:
1475 599 422 305 204 93 16 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 757 1174 2194 3123
Size: 757 417 1020 929
Fragments arranged by size:
1020 929 757 417
EcoICRI GAG'CTC
Cuts at: 0 807 1221 1635 1923 2421 3123
Size: 807 414 414 288 498 702
Fragments arranged by size:
807 702 498 414 414 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 292 337 499 544 757 895 1036
Size: 40 252 45 162 45 213 138 141
Cuts at: 1036 1090 1096 1637 1742 1867 3022 3046 3123
Size: 54 6 541 105 125 1155 24 77
Fragments arranged by size:
1155 541 252 213 162 141 138 125
105 77 54 45 45 40 24 6
EcoO109I rG'GnC_Cy
Cuts at: 0 264 519 732 1025 1574 1641 3123
Size: 264 255 213 293 549 67 1482
Fragments arranged by size:
1482 549 293 264 255 213 67
EcoRV GAT'ATC
Cuts at: 0 1830 3123
Size: 1830 1293
FseI GG_CCGG'CC
Cuts at: 0 175 3123
Size: 175 2948
HaeII r_GCGC'y
Cuts at: 0 27 108 138 633 984 3123
Size: 27 81 30 495 351 2139
Fragments arranged by size:
2139 495 351 81 30 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 937 969 1024 1056 1114 1146 1444 1476
Size: 937 32 55 32 58 32 298 32
Cuts at: 1476 1619 1651 3123
Size: 143 32 1472
Fragments arranged by size:
1472 937 298 143 58 55 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2547 2607 2861 3123
Size: 2547 60 254 262
Fragments arranged by size:
2547 262 254 60
HindIII A'AGCT_T
Cuts at: 0 724 1414 1778 3123
Size: 724 690 364 1345
Fragments arranged by size:
1345 724 690 364
KpnI G_GTAC'C
Cuts at: 0 98 211 3123
Size: 98 113 2912
Fragments arranged by size:
2912 113 98
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1650 3123
Size: 458 1192 1473
Fragments arranged by size:
1473 1192 458
MslI CAynn'nnrTG
Cuts at: 0 1378 2062 2113 3123
Size: 1378 684 51 1010
Fragments arranged by size:
1378 1010 684 51
MspA1I CmG'CkG
Cuts at: 0 561 856 978 2385 2504 2751 3123
Size: 561 295 122 1407 119 247 372
Fragments arranged by size:
1407 561 372 295 247 122 119
NaeI GCC'GGC
Cuts at: 0 11 173 790 3123
Size: 11 162 617 2333
Fragments arranged by size:
2333 617 162 11
NcoI C'CATG_G
Cuts at: 0 662 3123
Size: 662 2461
NdeI CA'TA_TG
Cuts at: 0 2355 3123
Size: 2355 768
NgoMIV G'CCGG_C
Cuts at: 0 9 171 788 3123
Size: 9 162 617 2335
Fragments arranged by size:
2335 617 162 9
NspI r_CATG'y
Cuts at: 0 900 1965 3123
Size: 900 1065 1158
Fragments arranged by size:
1158 1065 900
PasI CC'CwG_GG
Cuts at: 0 964 1822 3123
Size: 964 858 1301
Fragments arranged by size:
1301 964 858
PfoI T'CCnGG_A
Cuts at: 0 587 1505 1883 1916 2339 3123
Size: 587 918 378 33 423 784
Fragments arranged by size:
918 784 587 423 378 33
PpuMI rG'GwC_Cy
Cuts at: 0 519 732 1574 1641 3123
Size: 519 213 842 67 1482
Fragments arranged by size:
1482 842 519 213 67
PsiI TTA'TAA
Cuts at: 0 3047 3123
Size: 3047 76
PspOMI G'GGCC_C
Cuts at: 0 263 1025 3123
Size: 263 762 2098
Fragments arranged by size:
2098 762 263
PstI C_TGCA'G
Cuts at: 0 2143 2193 3123
Size: 2143 50 930
Fragments arranged by size:
2143 930 50
PvuII CAG'CTG
Cuts at: 0 856 2385 2504 2751 3123
Size: 856 1529 119 247 372
Fragments arranged by size:
1529 856 372 247 119
SacI G_AGCT'C
Cuts at: 0 809 1223 1637 1925 2423 3123
Size: 809 414 414 288 498 700
Fragments arranged by size:
809 700 498 414 414 288
SapI GCTCTTCn'nnn_
Cuts at: 0 2086 3107 3123
Size: 2086 1021 16
Fragments arranged by size:
2086 1021 16
SexAI A'CCwGG_T
Cuts at: 0 2633 3123
Size: 2633 490
SfcI C'TryA_G
Cuts at: 0 1434 2139 2189 3123
Size: 1434 705 50 934
Fragments arranged by size:
1434 934 705 50
SgrAI Cr'CCGG_yG
Cuts at: 0 9 3123
Size: 9 3114
SmaI CCC'GGG
Cuts at: 0 178 262 841 1399 2734 3123
Size: 178 84 579 558 1335 389
Fragments arranged by size:
1335 579 558 389 178 84
SmlI C'TyrA_G
Cuts at: 0 634 727 1636 2239 2299 3123
Size: 634 93 909 603 60 824
Fragments arranged by size:
909 824 634 603 93 60
SrfI GCCC'GGGC
Cuts at: 0 262 3123
Size: 262 2861
StuI AGG'CCT
Cuts at: 0 2149 3123
Size: 2149 974
StyI C'CwwG_G
Cuts at: 0 155 662 902 1751 2412 3053 3123
Size: 155 507 240 849 661 641 70
Fragments arranged by size:
849 661 641 507 240 155 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1155 1303 1366 1396 3123
Size: 1155 148 63 30 1727
Fragments arranged by size:
1727 1155 148 63 30
TatI w'GTAC_w
Cuts at: 0 2498 2706 3123
Size: 2498 208 417
Fragments arranged by size:
2498 417 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 378 410 472 489 504 521 1740 1772
Size: 378 32 62 17 15 17 1219 32
Cuts at: 1772 3123
Size: 1351
Fragments arranged by size:
1351 1219 378 62 32 32 17 17
15
Tth111I GACn'n_nGTC
Cuts at: 0 2578 3123
Size: 2578 545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1059 3123
Size: 1059 2064
XmaI C'CCGG_G
Cuts at: 0 176 260 839 1397 2732 3123
Size: 176 84 579 558 1335 391
Fragments arranged by size:
1335 579 558 391 176 84
XmnI GAAnn'nnTTC
Cuts at: 0 512 2200 3123
Size: 512 1688 923
Fragments arranged by size:
1688 923 512
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlfI AloI AlwNI
ApaI AvaI BamHI BanI BanII BbsI BbvCI BclI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRV FseI HaeII Hin4I HincII
HindIII KpnI MmeI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PasI PfoI PpuMI PsiI PspOMI PstI
PvuII SacI SapI SexAI SfcI SgrAI SmaI SmlI
SrfI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BfrBI BglII BmtI BsaAI BsaBI BsiEI BsiWI
BsmI BsmBI BspEI BssHII BstAPI BtgZI DraI EagI
EcoNI EcoRI FalI FspI FspAI HpaI KasI MfeI
MluI MscI NarI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SnaBI SpeI SphI SspI SwaI XbaI
XhoI ZraI