Restriction map for
pFN21AA0542
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AseI AsnI
Asp718I AspEI BciVI BclI BfrI
BglII BsaBI BsiEI BsiWI BsmBI
BsmI BspDI BspLU11I BstEII BstPI
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1293 GTATAC GT^[AC][GT]AC
AccIII 173 TCCGGA T^CCGGA
AcsI 1738 GAATTC [GA]^AATT[CT]
AflIII 1438 ACACGT A^C[GA][CT]GT
AocI 3484 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2183 AGCGCT AGC^GCT
ApoI 1738 GAATTC [GA]^AATT[CT]
AscI 1058 GGCGCGCC GG^CGCGCC
AspI 135 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1997 CCCGAG C^[CT]CG[GA]G
BamHI 170 GGATCC G^GATCC
BglI 3307 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 173 TCCGGA [AT]^CCGG[AT]
BseAI 173 TCCGGA T^CCGGA
BsoBI 1997 CCCGAG C^[CT]CG[GA]G
Bsp1407I 458 TGTACA T^GTACA
BspEI 173 TCCGGA T^CCGGA
BspHI 540 TCATGA T^CATGA
BsrDI 2793 CATTGC GCAATG(2/0)
BsrGI 458 TGTACA T^GTACA
BssHII 1059 GCGCGC G^CGCGC
BssSI 409 CACGAG CACGAG(-5/-1)
Bst1107I 1293 GTATAC GTA^TAC
BstZ17I 1293 GTATAC GTA^TAC
Bsu36I 3484 CCTGAGG CC^T[ACGT]AGG
DraIII 1849 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2183 AGCGCT AGC^GCT
Eco81I 3484 CCTGAGG CC^T[ACGT]AGG
EcoRI 1738 GAATTC G^AATTC
HindIII 1667 AAGCTT A^AGCTT
MroI 173 TCCGGA T^CCGGA
NcoI 1991 CCATGG C^CATGG
NdeI 2052 CATATG CA^TATG
PflFI 135 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 540 TCATGA T^CATGA
SapI 1129 GAAGAGC GCTCTTC(1/4)
ScaI 2032 AGTACT AGT^ACT
SexAI 2173 ACCTGGT A^CC[AT]GGT
SphI 1230 GCATGC GCATG^C
SspBI 458 TGTACA T^GTACA
Tth111I 135 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1991 CCATGGCCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 366 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1665 GAAAGCTTTC
AvrII 3000 CCTAGG C^CTAGG
3229 CCTAGG
BbeI 1403 GGCGCC GGCGC^C
3589 GGCGCC
BbrPI 675 CACGTG CAC^GTG
2481 CACGTG
BbvCI 2728 CCTCAGC CCTCAGC(-5/-2)
2878 GCTGAGG
BcgI 3437 CGAGCAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1268 GCAAATGTATCG
BlnI 3000 CCTAGG C^CTAGG
3229 CCTAGG
BsaI 2824 GAGACC GGTCTC(1/5)
1184 GGTCTC
BsrFI 242 GCCGGT [GA]^CCGG[CT]
2255 ACCGGC
BstBI 299 TTCGAA TT^CGAA
373 TTCGAA
Cfr10I 242 GCCGGT [GA]^CCGG[CT]
2255 ACCGGC
DsaI 1650 CCACGG C^C[GA][CT]GG
1991 CCATGG
KasI 1403 GGCGCC G^GCGCC
3589 GGCGCC
MslI 964 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1259 CACTGCTGTG
NarI 1403 GGCGCC GG^CGCC
3589 GGCGCC
NspI 1085 ACATGC [GA]CATG^[CT]
1230 GCATGC
NspV 299 TTCGAA TT^CGAA
373 TTCGAA
PleI 1391 GACTC GAGTC(4/5)
2360 GACTC
PmaCI 675 CACGTG CAC^GTG
2481 CACGTG
PmlI 675 CACGTG CAC^GTG
2481 CACGTG
PpuMI 2063 AGGTCCT [GA]G^G[AT]CC[CT]
2775 GGGTCCC
SfoI 1403 GGCGCC GGC^GCC
3589 GGCGCC
SfuI 299 TTCGAA TT^CGAA
373 TTCGAA
XmnI 366 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1665 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
AviII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII