(Circular) (Six-base) MAPSORT of: pFN21AA0542.seq Check: 9297 from: 1 to: 8338 pFN21AA0542 8338 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5445 5503 5445 Size: 58 8280 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6148 278 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 5681 1937 Size: 1362 2382 4594 Fragments arranged by size: 4594 2382 1362 Acc65I G'GTAC_C Cuts at: 5659 5659 Size: 8338 AclI AA'CG_TT Cuts at: 1563 6465 6838 1563 Size: 4902 373 3063 Fragments arranged by size: 4902 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2603 6401 7785 8053 1632 Size: 971 3798 1384 268 1917 Fragments arranged by size: 3798 1917 1384 971 268 AfeI AGC'GCT Cuts at: 4190 4190 Size: 8338 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8141 AflIII A'CryG_T Cuts at: 1203 3443 7237 8214 1203 Size: 2240 3794 977 1327 Fragments arranged by size: 3794 2240 1327 977 AgeI A'CCGG_T Cuts at: 7169 7169 Size: 8338 AhdI GACnn_n'nnGTC Cuts at: 7067 7067 Size: 8338 AleI CACnn'nnGTG Cuts at: 1166 2973 3268 1166 Size: 1807 295 6236 Fragments arranged by size: 6236 1807 295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3286 4367 1184 Size: 2102 1081 5155 Fragments arranged by size: 5155 2102 1081 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4045 4077 5408 5440 4045 Size: 32 1331 32 6943 Fragments arranged by size: 6943 1331 32 32 AlwNI CAG_nnn'CTG Cuts at: 2797 3160 3174 3399 3565 3763 3826 3961 4167 Size: 363 14 225 166 198 63 135 206 Cuts at: 4167 4228 4270 4630 4741 5489 5557 5620 5632 Size: 61 42 360 111 748 68 63 12 Cuts at: 5632 6081 7653 2797 Size: 449 1572 3482 Fragments arranged by size: 3482 1572 748 449 363 360 225 206 198 166 135 111 68 63 63 61 42 14 12 ApaI G_GGCC'C Cuts at: 2752 4444 5101 2752 Size: 1692 657 5989 Fragments arranged by size: 5989 1692 657 ApaLI G'TGCA_C Cuts at: 6395 7551 6395 Size: 1156 7182 ApoI r'AATT_y Cuts at: 1493 3743 5647 5873 6025 8134 1493 Size: 2250 1904 226 152 2109 1697 Fragments arranged by size: 2250 2109 1904 1697 226 152 AscI GG'CGCG_CC Cuts at: 3064 3064 Size: 8338 AseI AT'TA_AT Cuts at: 160 1032 6892 160 Size: 872 5860 1606 Fragments arranged by size: 5860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 AvaI C'yCGr_G Cuts at: 1870 1943 4002 5663 1870 Size: 73 2059 1661 4545 Fragments arranged by size: 4545 2059 1661 73 AvrII C'CTAG_G Cuts at: 5005 5234 5005 Size: 229 8109 BamHI G'GATC_C Cuts at: 2175 5668 6035 2175 Size: 3493 367 4478 Fragments arranged by size: 4478 3493 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3408 3588 4577 4820 5594 Size: 325 217 627 1621 180 989 243 774 Cuts at: 5594 5659 7114 618 Size: 65 1455 1842 Fragments arranged by size: 1842 1621 1455 989 774 627 325 243 217 180 65 BanII G_rGCy'C Cuts at: 1391 2752 3367 3708 4444 4867 4893 4908 5101 Size: 1361 615 341 736 423 26 15 193 Cuts at: 5101 5571 5657 1391 Size: 470 86 4072 Fragments arranged by size: 4072 1361 736 615 470 423 341 193 86 26 15 BbeI G_GCGC'C Cuts at: 3412 5598 3412 Size: 2186 6152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 2368 2495 4301 928 Size: 971 252 217 127 1806 4965 Fragments arranged by size: 4965 1806 971 252 217 127 BbvCI CC'TCA_GC Cuts at: 4734 4884 4734 Size: 150 8188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3262 3296 5431 5465 6529 6563 1903 Size: 34 1325 34 2135 34 1064 34 3678 Fragments arranged by size: 3678 2135 1325 1064 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6230 7440 6230 Size: 1210 7128 BclI T'GATC_A Cuts at: 1548 1548 Size: 8338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5318 6949 136 Size: 107 122 71 631 605 3646 1631 1525 Fragments arranged by size: 3646 1631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8333 8333 Size: 8338 BlpI GC'TnA_GC Cuts at: 2934 5016 5577 5755 2934 Size: 2082 561 178 5517 Fragments arranged by size: 5517 2082 561 178 Bme1580I G_kGCm'C Cuts at: 1790 2722 2752 3646 3700 4444 5101 6399 7555 Size: 932 30 894 54 744 657 1298 1156 Cuts at: 7555 1790 Size: 2573 Fragments arranged by size: 2573 1298 1156 932 894 744 657 54 30 BmgBI CAC'GTC Cuts at: 1318 2484 3608 1318 Size: 1166 1124 6048 Fragments arranged by size: 6048 1166 1124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3112 6069 7027 Size: 416 53 83 399 24 1695 2957 958 Cuts at: 7027 442 Size: 1753 Fragments arranged by size: 2957 1753 1695 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3239 3239 Size: 8338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2660 2744 2945 3508 3706 3796 4256 4295 Size: 1421 84 201 563 198 90 460 39 Cuts at: 4295 4529 4553 4922 5483 5555 6998 1239 Size: 234 24 369 561 72 1443 2579 Fragments arranged by size: 2579 1443 1421 563 561 460 369 234 201 198 90 84 72 39 24 Bpu10I CC'TnA_GC Cuts at: 2753 4734 4884 2753 Size: 1981 150 6207 Fragments arranged by size: 6207 1981 150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3650 4847 5378 5390 6463 7328 7626 3650 Size: 1197 531 12 1073 865 298 4362 Fragments arranged by size: 4362 1197 1073 865 531 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3195 4823 7001 882 Size: 617 1696 1628 2178 2219 Fragments arranged by size: 2219 2178 1696 1628 617 BsaAI yAC'GTr Cuts at: 493 1204 2596 2682 4488 6129 493 Size: 711 1392 86 1806 1641 2702 Fragments arranged by size: 2702 1806 1641 1392 711 86 BsaBI GATnn'nnATC Cuts at: 8332 8332 Size: 8338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 4942 5595 6145 Size: 53 83 186 936 1876 1533 653 550 Cuts at: 6145 6527 7162 275 Size: 382 635 1451 Fragments arranged by size: 1876 1533 1451 936 653 635 550 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2178 6771 7169 7443 7590 8001 2178 Size: 4593 398 274 147 411 2515 Fragments arranged by size: 4593 2515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2996 3026 3608 3638 3679 Size: 30 165 30 1593 30 582 30 41 Cuts at: 3679 3709 1178 Size: 30 5807 Fragments arranged by size: 5807 1593 582 165 41 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 4528 4556 4901 5387 5492 5579 1186 Size: 1222 2120 28 345 486 105 87 3945 Fragments arranged by size: 3945 2120 1222 486 345 105 87 28 BseYI C'CCAG_C Cuts at: 3756 4218 4638 4714 4757 4805 4811 5321 7541 Size: 462 420 76 43 48 6 510 2220 Cuts at: 7541 8037 3756 Size: 496 4057 Fragments arranged by size: 4057 2220 510 496 462 420 76 48 43 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2133 2231 2786 3123 3149 3827 3848 Size: 573 397 98 555 337 26 678 21 Cuts at: 3848 4121 4316 5184 5378 5537 1163 Size: 273 195 868 194 159 3964 Fragments arranged by size: 3964 868 678 573 555 397 337 273 195 194 159 98 26 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5788 6549 7577 1540 Size: 275 186 3787 761 1028 2301 Fragments arranged by size: 3787 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2681 2961 3141 4346 4867 4908 5571 6399 6484 Size: 280 180 1205 521 41 663 828 85 Cuts at: 6484 7555 2681 Size: 1071 3464 Fragments arranged by size: 3464 1205 1071 828 663 521 280 180 85 41 BsmI GAATG_Cn' Cuts at: 5849 5942 5849 Size: 93 8245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2681 2722 2752 2961 3141 3367 3646 Size: 399 891 41 30 209 180 226 279 Cuts at: 3646 3700 3708 4346 4444 4867 4893 4908 5101 Size: 54 8 638 98 423 26 15 193 Cuts at: 5101 5571 5657 6399 6484 7555 1391 Size: 470 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 891 742 638 470 423 399 279 226 209 193 180 98 86 85 54 41 30 26 15 8 BspEI T'CCGG_A Cuts at: 2178 8001 2178 Size: 5823 2515 BspHI T'CATG_A Cuts at: 2545 6227 7957 2545 Size: 3682 1730 2926 Fragments arranged by size: 3682 2926 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2901 3849 4385 4636 5445 5503 5693 844 Size: 2057 948 536 251 809 58 190 3489 Fragments arranged by size: 3489 2057 948 809 536 251 190 58 BsrBI CCG'CTC Cuts at: 1394 1823 2998 3341 3812 3817 6225 1394 Size: 429 1175 343 471 5 2408 3507 Fragments arranged by size: 3507 2408 1175 471 429 343 5 BsrDI GCAATG_nn' Cuts at: 65 1248 4797 6833 7007 65 Size: 1183 3549 2036 174 1396 Fragments arranged by size: 3549 2036 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2247 4260 6982 7169 1700 Size: 110 437 2013 2722 187 2869 Fragments arranged by size: 2869 2722 2013 437 187 110 BsrGI T'GTAC_A Cuts at: 96 2463 96 Size: 2367 5971 BssHII G'CGCG_C Cuts at: 1925 3064 1925 Size: 1139 7199 BssSI C'ACGA_G Cuts at: 2414 6398 7410 2414 Size: 3984 1012 3342 Fragments arranged by size: 3984 3342 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2271 2563 3859 3961 4228 4270 4306 4630 Size: 1477 292 1296 102 267 42 36 324 Cuts at: 4630 5392 5515 794 Size: 762 123 3617 Fragments arranged by size: 3617 1477 1296 762 324 292 267 123 102 42 36 BstBI TT'CG_AA Cuts at: 2305 2379 7142 2305 Size: 74 4763 3501 Fragments arranged by size: 4763 3501 74 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8338 BstXI CCAn_nnnn'nTGG Cuts at: 3052 4072 4492 5591 3052 Size: 1020 420 1099 5799 Fragments arranged by size: 5799 1099 1020 420 BstYI r'GATC_y Cuts at: 1972 2175 5376 5547 5668 6035 6420 6437 7889 Size: 203 3201 171 121 367 385 17 1452 Cuts at: 7889 7975 7987 8333 1972 Size: 86 12 346 1977 Fragments arranged by size: 3201 1977 1452 385 367 346 203 171 121 86 17 12 BstZ17I GTA'TAC Cuts at: 3300 3300 Size: 8338 Bsu36I CC'TnA_GG Cuts at: 5490 5490 Size: 8338 BtgI C'CryG_G Cuts at: 513 1065 3655 3996 513 Size: 552 2590 341 4855 Fragments arranged by size: 4855 2590 552 341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3418 4735 487 Size: 683 56 2192 1317 4090 Fragments arranged by size: 4090 2192 1317 683 56 BtsI GCAGTG_nn' Cuts at: 2841 3263 4251 4316 4541 4911 5854 6648 6668 Size: 422 988 65 225 370 943 794 20 Cuts at: 6668 2841 Size: 4511 Fragments arranged by size: 4511 988 943 794 422 370 225 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8303 DraI TTT'AAA Cuts at: 5642 5642 Size: 8338 DraIII CAC_nnn'GTG Cuts at: 3859 3859 Size: 8338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7345 809 Size: 507 6029 1802 Fragments arranged by size: 6029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 3042 3537 Size: 54 1425 50 275 18 447 765 495 Cuts at: 3537 5265 5785 6674 8303 8 Size: 1728 520 889 1629 43 Fragments arranged by size: 1728 1629 1425 889 765 520 495 447 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5785 1537 Size: 275 3973 4090 Fragments arranged by size: 4090 3973 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2314 2906 3129 3302 4316 6268 1353 Size: 961 592 223 173 1014 1952 3423 Fragments arranged by size: 3423 1952 1014 961 592 223 173 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4373 4670 6925 7299 7445 387 Size: 1064 138 2784 297 2255 374 146 1280 Fragments arranged by size: 2784 2255 1280 1064 374 297 146 138 EcoICRI GAG'CTC Cuts at: 4865 4906 5569 4865 Size: 41 663 7634 Fragments arranged by size: 7634 663 41 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 2660 2744 2945 3508 3706 3796 Size: 393 971 57 84 201 563 198 90 Cuts at: 3796 4256 4295 4529 4553 4922 5483 5555 6401 Size: 460 39 234 24 369 561 72 846 Cuts at: 6401 6998 7785 8053 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 971 846 787 597 563 561 460 393 369 268 234 201 198 90 84 72 57 39 24 EcoNI CCTnn'n_nnAGG Cuts at: 3920 4571 5054 5624 3920 Size: 651 483 570 6634 Fragments arranged by size: 6634 651 570 483 EcoO109I rG'GnC_Cy Cuts at: 2748 2749 4069 4441 4781 5059 5097 5098 5486 Size: 1 1320 372 340 278 38 1 388 Cuts at: 5486 5617 5629 2748 Size: 131 12 5457 Fragments arranged by size: 5457 1320 388 372 340 278 131 38 12 1 1 EcoRI G'AATT_C Cuts at: 3743 5647 6025 3743 Size: 1904 378 6056 Fragments arranged by size: 6056 1904 378 FspI TGC'GCA Cuts at: 2264 4968 5453 6844 2264 Size: 2704 485 1391 3758 Fragments arranged by size: 3758 2704 1391 485 HaeII r_GCGC'y Cuts at: 1678 3412 3875 4192 4544 5112 5584 5598 7485 Size: 1734 463 317 352 568 472 14 1887 Cuts at: 7485 1678 Size: 2531 Fragments arranged by size: 2531 1887 1734 568 472 463 352 317 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2620 2652 4288 4320 6979 Size: 32 135 32 1048 32 1636 32 2659 Cuts at: 6979 7011 7053 7085 1373 Size: 32 42 32 2626 Fragments arranged by size: 2659 2626 1636 1048 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5682 5928 669 Size: 988 281 3744 246 3079 Fragments arranged by size: 3744 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3672 748 Size: 2924 5414 HpaI GTT'AAC Cuts at: 5928 5928 Size: 8338 KasI G'GCGC_C Cuts at: 3408 5594 3408 Size: 2186 6152 KpnI G_GTAC'C Cuts at: 5663 5663 Size: 8338 MfeI C'AATT_G Cuts at: 5937 5937 Size: 8338 MluI A'CGCG_T Cuts at: 8214 8214 Size: 8338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2172 4938 7452 7636 1305 Size: 867 2766 2514 184 2007 Fragments arranged by size: 2766 2514 2007 867 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 3044 3539 5267 10 Size: 54 1425 343 447 765 495 1728 3081 Fragments arranged by size: 3081 1728 1425 765 495 447 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2973 3268 6296 6655 6814 518 Size: 648 1807 295 3028 359 159 2042 Fragments arranged by size: 3028 2042 1807 648 359 295 159 MspA1I CmG'CkG Cuts at: 1606 3157 3325 3958 4234 4267 4402 4426 4630 Size: 1551 168 633 276 33 135 24 204 Cuts at: 4630 4686 5224 5554 5754 6431 7579 7824 1606 Size: 56 538 330 200 677 1148 245 2120 Fragments arranged by size: 2120 1551 1148 677 633 538 330 276 245 204 200 168 135 56 33 24 NarI GG'CG_CC Cuts at: 3409 5595 3409 Size: 2186 6152 NcoI C'CATG_G Cuts at: 513 1065 3996 513 Size: 552 2931 4855 Fragments arranged by size: 4855 2931 552 NdeI CA'TA_TG Cuts at: 387 4058 387 Size: 3671 4667 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8338 NotI GC'GGCC_GC Cuts at: 5785 5785 Size: 8338 NspI r_CATG'y Cuts at: 1132 1229 3094 3239 5696 7241 1132 Size: 97 1865 145 2457 1545 2229 Fragments arranged by size: 2457 2229 1865 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8338 PciI A'CATG_T Cuts at: 7237 7237 Size: 8338 PflMI CCAn_nnn'nTGG Cuts at: 1108 4354 4698 4900 5557 1108 Size: 3246 344 202 657 3889 Fragments arranged by size: 3889 3246 657 344 202 PfoI T'CCnGG_A Cuts at: 3918 4569 3918 Size: 651 7687 PmeI GTTT'AAAC Cuts at: 5642 5642 Size: 8338 PmlI CAC'GTG Cuts at: 2682 4488 2682 Size: 1806 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2526 2558 6407 6439 7920 7952 2526 Size: 32 3849 32 1481 32 2912 Fragments arranged by size: 3849 2912 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4069 4781 4069 Size: 712 7626 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8338 PsiI TTA'TAA Cuts at: 5908 5908 Size: 8338 PspOMI G'GGCC_C Cuts at: 2748 4440 5097 2748 Size: 1692 657 5989 Fragments arranged by size: 5989 1692 657 PstI C_TGCA'G Cuts at: 830 2836 3879 4205 4233 4311 4470 4536 4635 Size: 2006 1043 326 28 78 159 66 99 Cuts at: 4635 5344 5690 830 Size: 709 346 3478 Fragments arranged by size: 3478 2006 1043 709 346 326 159 99 78 66 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8338 PvuII CAG'CTG Cuts at: 3157 3325 3958 4234 4267 4402 4630 5554 3157 Size: 168 633 276 33 135 228 924 5941 Fragments arranged by size: 5941 924 633 276 228 168 135 33 SacI G_AGCT'C Cuts at: 4867 4908 5571 4867 Size: 41 663 7634 Fragments arranged by size: 7634 663 41 SalI G'TCGA_C Cuts at: 1936 5680 1936 Size: 3744 4594 SanDI GG'GwC_CC Cuts at: 4781 4781 Size: 8338 SapI GCTCTTCn'nnn_ Cuts at: 3129 3129 Size: 8338 SbfI CC_TGCA'GG Cuts at: 5690 5690 Size: 8338 ScaI AGT'ACT Cuts at: 4039 6586 8298 4039 Size: 2547 1712 4079 Fragments arranged by size: 4079 2547 1712 SexAI A'CCwGG_T Cuts at: 4178 4178 Size: 8338 SfcI C'TryA_G Cuts at: 826 1045 2449 2631 2832 3253 3293 3329 3875 Size: 219 1404 182 201 421 40 36 546 Cuts at: 3875 4201 4229 4307 4466 4532 4631 5340 5686 Size: 326 28 78 159 66 99 709 346 Cuts at: 5686 6821 7502 7693 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1404 1135 709 681 546 421 346 326 219 201 191 182 159 99 78 66 40 36 28 SfoI GGC'GCC Cuts at: 3410 5596 3410 Size: 2186 6152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8338 SmaI CCC'GGG Cuts at: 1872 5665 1872 Size: 3793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3665 4826 5357 5369 6442 7343 Size: 197 926 1722 1161 531 12 1073 901 Cuts at: 7343 7605 820 Size: 262 1553 Fragments arranged by size: 1722 1553 1161 1073 926 901 531 262 197 12 SnaBI TAC'GTA Cuts at: 493 6129 493 Size: 5636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8338 SphI G_CATG'C Cuts at: 1132 3239 5696 1132 Size: 2107 2457 3774 Fragments arranged by size: 3774 2457 2107 SspI AAT'ATT Cuts at: 5 52 6262 5 Size: 47 6210 2081 Fragments arranged by size: 6210 2081 47 StuI AGG'CCT Cuts at: 1517 2573 2879 2990 4550 4616 4981 1517 Size: 1056 306 111 1560 66 365 4874 Fragments arranged by size: 4874 1560 1056 365 306 111 66 StyI C'CwwG_G Cuts at: 513 1065 3700 3996 4065 5005 5234 5777 513 Size: 552 2635 296 69 940 229 543 3074 Fragments arranged by size: 3074 2635 940 552 543 296 229 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3874 4361 4771 6365 6382 6535 6720 Size: 1255 2294 487 410 1594 17 153 185 Cuts at: 6720 7165 325 Size: 445 1498 Fragments arranged by size: 2294 1594 1498 1255 487 445 410 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2463 2526 4037 Size: 274 80 33 51 1443 486 63 1511 Cuts at: 4037 4501 6584 8296 96 Size: 464 2083 1712 138 Fragments arranged by size: 2083 1712 1511 1443 486 464 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2413 2445 3608 3640 3679 3711 8298 8330 2413 Size: 32 1163 32 39 32 4587 32 2421 Fragments arranged by size: 4587 2421 1163 39 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2143 1316 Size: 827 7511 XbaI T'CTAG_A Cuts at: 5674 5674 Size: 8338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4003 4003 Size: 8338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8338 XmaI C'CCGG_G Cuts at: 1870 5663 1870 Size: 3793 4545 XmnI GAAnn'nnTTC Cuts at: 2375 3674 6467 2375 Size: 1299 2793 4246 Fragments arranged by size: 4246 2793 1299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6146 276 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: BaeI BfrBI BsiWI BsmBI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SrfI SwaI