Restriction map for
pFN21AA0542
(Linear) (Six-base) MAPSORT of: KIAA0542.seq Check: 871 from: 1 to: 3633
KIAA0542.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3440 3498 3633
Size: 3440 58 135
Fragments arranged by size:
3440 135 58
AccI GT'mk_AC
Cuts at: 0 1294 3633
Size: 1294 2339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 3633
Size: 598 3035
AfeI AGC'GCT
Cuts at: 0 2185 3633
Size: 2185 1448
AflIII A'CryG_T
Cuts at: 0 1438 3633
Size: 1438 2195
AleI CACnn'nnGTG
Cuts at: 0 968 1263 3633
Size: 968 295 2370
Fragments arranged by size:
2370 968 295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1281 2362 3633
Size: 1281 1081 1271
Fragments arranged by size:
1281 1271 1081
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2040 2072 3403 3435 3633
Size: 2040 32 1331 32 198
Fragments arranged by size:
2040 1331 198 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 792 1155 1169 1394 1560 1758 1821 1956
Size: 792 363 14 225 166 198 63 135
Cuts at: 1956 2162 2223 2265 2625 2736 3484 3552 3615
Size: 206 61 42 360 111 748 68 63
Cuts at: 3615 3627 3633
Size: 12 6
Fragments arranged by size:
792 748 363 360 225 206 198 166
135 111 68 63 63 61 42 14
12 6
ApaI G_GGCC'C
Cuts at: 0 747 2439 3096 3633
Size: 747 1692 657 537
Fragments arranged by size:
1692 747 657 537
ApoI r'AATT_y
Cuts at: 0 1738 3633
Size: 1738 1895
AscI GG'CGCG_CC
Cuts at: 0 1059 3633
Size: 1059 2574
AvaI C'yCGr_G
Cuts at: 0 1997 3633
Size: 1997 1636
AvrII C'CTAG_G
Cuts at: 0 3000 3229 3633
Size: 3000 229 404
Fragments arranged by size:
3000 404 229
BamHI G'GATC_C
Cuts at: 0 170 3633
Size: 170 3463
BanI G'GyrC_C
Cuts at: 0 1403 1583 2572 2815 3589 3633
Size: 1403 180 989 243 774 44
Fragments arranged by size:
1403 989 774 243 180 44
BanII G_rGCy'C
Cuts at: 0 747 1362 1703 2439 2862 2888 2903 3096
Size: 747 615 341 736 423 26 15 193
Cuts at: 3096 3566 3633
Size: 470 67
Fragments arranged by size:
747 736 615 470 423 341 193 67
26 15
BbeI G_GCGC'C
Cuts at: 0 1407 3593 3633
Size: 1407 2186 40
Fragments arranged by size:
2186 1407 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 363 490 2296 3633
Size: 146 217 127 1806 1337
Fragments arranged by size:
1806 1337 217 146 127
BbvCI CC'TCA_GC
Cuts at: 0 2729 2879 3633
Size: 2729 150 754
Fragments arranged by size:
2729 754 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1257 1291 3426 3460 3633
Size: 1257 34 2135 34 173
Fragments arranged by size:
2135 1257 173 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 3313 3633
Size: 3313 320
BlpI GC'TnA_GC
Cuts at: 0 929 3011 3572 3633
Size: 929 2082 561 61
Fragments arranged by size:
2082 929 561 61
Bme1580I G_kGCm'C
Cuts at: 0 717 747 1641 1695 2439 3096 3633
Size: 717 30 894 54 744 657 537
Fragments arranged by size:
894 744 717 657 537 54 30
BmgBI CAC'GTC
Cuts at: 0 479 1603 3633
Size: 479 1124 2030
Fragments arranged by size:
2030 1124 479
BmrI ACTGGGnnnn_n'
Cuts at: 0 1107 3633
Size: 1107 2526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1234 3633
Size: 1234 2399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 739 940 1503 1701 1791 2251 2290
Size: 655 84 201 563 198 90 460 39
Cuts at: 2290 2524 2548 2917 3478 3550 3633
Size: 234 24 369 561 72 83
Fragments arranged by size:
655 563 561 460 369 234 201 198
90 84 83 72 39 24
Bpu10I CC'TnA_GC
Cuts at: 0 748 2729 2879 3633
Size: 748 1981 150 754
Fragments arranged by size:
1981 754 748 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1645 2842 3373 3385 3633
Size: 1645 1197 531 12 248
Fragments arranged by size:
1645 1197 531 248 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 1190 2818 3633
Size: 1190 1628 815
Fragments arranged by size:
1628 1190 815
BsaAI yAC'GTr
Cuts at: 0 591 677 2483 3633
Size: 591 86 1806 1150
Fragments arranged by size:
1806 1150 591 86
BsaHI Gr'CG_yC
Cuts at: 0 1404 2937 3590 3633
Size: 1404 1533 653 43
Fragments arranged by size:
1533 1404 653 43
BsaWI w'CCGG_w
Cuts at: 0 173 3633
Size: 173 3460
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 991 1021 1603 1633 1674 1704 3633
Size: 991 30 582 30 41 30 1929
Fragments arranged by size:
1929 991 582 41 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 2523 2551 2896 3382 3487 3574 3633
Size: 403 2120 28 345 486 105 87 59
Fragments arranged by size:
2120 486 403 345 105 87 59 28
BseYI C'CCAG_C
Cuts at: 0 1751 2213 2633 2709 2752 2800 2806 3316
Size: 1751 462 420 76 43 48 6 510
Cuts at: 3316 3633
Size: 317
Fragments arranged by size:
1751 510 462 420 317 76 48 43
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 226 781 1118 1144 1822 1843 2116
Size: 128 98 555 337 26 678 21 273
Cuts at: 2116 2311 3179 3373 3532 3633
Size: 195 868 194 159 101
Fragments arranged by size:
868 678 555 337 273 195 194 159
128 101 98 26 21
BsiHKAI G_wGCw'C
Cuts at: 0 676 956 1136 2341 2862 2903 3566 3633
Size: 676 280 180 1205 521 41 663 67
Fragments arranged by size:
1205 676 663 521 280 180 67 41
Bsp1286I G_dGCh'C
Cuts at: 0 676 717 747 956 1136 1362 1641 1695
Size: 676 41 30 209 180 226 279 54
Cuts at: 1695 1703 2341 2439 2862 2888 2903 3096 3566
Size: 8 638 98 423 26 15 193 470
Cuts at: 3566 3633
Size: 67
Fragments arranged by size:
676 638 470 423 279 226 209 193
180 98 67 54 41 30 26 15
8
BspEI T'CCGG_A
Cuts at: 0 173 3633
Size: 173 3460
BspHI T'CATG_A
Cuts at: 0 540 3633
Size: 540 3093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 896 1844 2380 2631 3440 3498 3633
Size: 896 948 536 251 809 58 135
Fragments arranged by size:
948 896 809 536 251 135 58
BsrBI CCG'CTC
Cuts at: 0 993 1336 1807 1812 3633
Size: 993 343 471 5 1821
Fragments arranged by size:
1821 993 471 343 5
BsrDI GCAATG_nn'
Cuts at: 0 2792 3633
Size: 2792 841
BsrFI r'CCGG_y
Cuts at: 0 242 2255 3633
Size: 242 2013 1378
Fragments arranged by size:
2013 1378 242
BsrGI T'GTAC_A
Cuts at: 0 458 3633
Size: 458 3175
BssHII G'CGCG_C
Cuts at: 0 1059 3633
Size: 1059 2574
BssSI C'ACGA_G
Cuts at: 0 409 3633
Size: 409 3224
BstAPI GCAn_nnn'nTGC
Cuts at: 0 266 558 1854 1956 2223 2265 2301 2625
Size: 266 292 1296 102 267 42 36 324
Cuts at: 2625 3387 3510 3633
Size: 762 123 123
Fragments arranged by size:
1296 762 324 292 267 266 123 123
102 42 36
BstBI TT'CG_AA
Cuts at: 0 300 374 3633
Size: 300 74 3259
Fragments arranged by size:
3259 300 74
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1047 2067 2487 3586 3633
Size: 1047 1020 420 1099 47
Fragments arranged by size:
1099 1047 1020 420 47
BstYI r'GATC_y
Cuts at: 0 170 3371 3542 3633
Size: 170 3201 171 91
Fragments arranged by size:
3201 171 170 91
BstZ17I GTA'TAC
Cuts at: 0 1295 3633
Size: 1295 2338
Bsu36I CC'TnA_GG
Cuts at: 0 3485 3633
Size: 3485 148
BtgI C'CryG_G
Cuts at: 0 1650 1991 3633
Size: 1650 341 1642
Fragments arranged by size:
1650 1642 341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1413 2730 3633
Size: 1413 1317 903
Fragments arranged by size:
1413 1317 903
BtsI GCAGTG_nn'
Cuts at: 0 836 1258 2246 2311 2536 2906 3633
Size: 836 422 988 65 225 370 727
Fragments arranged by size:
988 836 727 422 370 225 65
DraIII CAC_nnn'GTG
Cuts at: 0 1854 3633
Size: 1854 1779
EaeI y'GGCC_r
Cuts at: 0 272 1037 1532 3260 3633
Size: 272 765 495 1728 373
Fragments arranged by size:
1728 765 495 373 272
EarI CTCTTCn'nnn_
Cuts at: 0 309 901 1124 1297 2311 3633
Size: 309 592 223 173 1014 1322
Fragments arranged by size:
1322 1014 592 309 223 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2368 2665 3633
Size: 2368 297 968
Fragments arranged by size:
2368 968 297
EcoICRI GAG'CTC
Cuts at: 0 2860 2901 3564 3633
Size: 2860 41 663 69
Fragments arranged by size:
2860 663 69 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 655 739 940 1503 1701 1791 2251
Size: 598 57 84 201 563 198 90 460
Cuts at: 2251 2290 2524 2548 2917 3478 3550 3633
Size: 39 234 24 369 561 72 83
Fragments arranged by size:
598 563 561 460 369 234 201 198
90 84 83 72 57 39 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1915 2566 3049 3619 3633
Size: 1915 651 483 570 14
Fragments arranged by size:
1915 651 570 483 14
EcoO109I rG'GnC_Cy
Cuts at: 0 743 744 2064 2436 2776 3054 3092 3093
Size: 743 1 1320 372 340 278 38 1
Cuts at: 3093 3481 3612 3624 3633
Size: 388 131 12 9
Fragments arranged by size:
1320 743 388 372 340 278 131 38
12 9 1 1
EcoRI G'AATT_C
Cuts at: 0 1738 3633
Size: 1738 1895
FspI TGC'GCA
Cuts at: 0 259 2963 3448 3633
Size: 259 2704 485 185
Fragments arranged by size:
2704 485 259 185
HaeII r_GCGC'y
Cuts at: 0 1407 1870 2187 2539 3107 3579 3593 3633
Size: 1407 463 317 352 568 472 14 40
Fragments arranged by size:
1407 568 472 463 352 317 40 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 615 647 2283 2315 3633
Size: 615 32 1636 32 1318
Fragments arranged by size:
1636 1318 615 32 32
HindIII A'AGCT_T
Cuts at: 0 1667 3633
Size: 1667 1966
KasI G'GCGC_C
Cuts at: 0 1403 3589 3633
Size: 1403 2186 44
Fragments arranged by size:
2186 1403 44
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 2933 3633
Size: 167 2766 700
Fragments arranged by size:
2766 700 167
MscI TGG'CCA
Cuts at: 0 274 1039 1534 3262 3633
Size: 274 765 495 1728 371
Fragments arranged by size:
1728 765 495 371 274
MslI CAynn'nnrTG
Cuts at: 0 968 1263 3633
Size: 968 295 2370
Fragments arranged by size:
2370 968 295
MspA1I CmG'CkG
Cuts at: 0 1152 1320 1953 2229 2262 2397 2421 2625
Size: 1152 168 633 276 33 135 24 204
Cuts at: 2625 2681 3219 3549 3633
Size: 56 538 330 84
Fragments arranged by size:
1152 633 538 330 276 204 168 135
84 56 33 24
NarI GG'CG_CC
Cuts at: 0 1404 3590 3633
Size: 1404 2186 43
Fragments arranged by size:
2186 1404 43
NcoI C'CATG_G
Cuts at: 0 1991 3633
Size: 1991 1642
NdeI CA'TA_TG
Cuts at: 0 2053 3633
Size: 2053 1580
NspI r_CATG'y
Cuts at: 0 1089 1234 3633
Size: 1089 145 2399
Fragments arranged by size:
2399 1089 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 2349 2693 2895 3552 3633
Size: 2349 344 202 657 81
Fragments arranged by size:
2349 657 344 202 81
PfoI T'CCnGG_A
Cuts at: 0 1913 2564 3633
Size: 1913 651 1069
Fragments arranged by size:
1913 1069 651
PmlI CAC'GTG
Cuts at: 0 677 2483 3633
Size: 677 1806 1150
Fragments arranged by size:
1806 1150 677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 521 553 3633
Size: 521 32 3080
Fragments arranged by size:
3080 521 32
PpuMI rG'GwC_Cy
Cuts at: 0 2064 2776 3633
Size: 2064 712 857
Fragments arranged by size:
2064 857 712
PspOMI G'GGCC_C
Cuts at: 0 743 2435 3092 3633
Size: 743 1692 657 541
Fragments arranged by size:
1692 743 657 541
PstI C_TGCA'G
Cuts at: 0 831 1874 2200 2228 2306 2465 2531 2630
Size: 831 1043 326 28 78 159 66 99
Cuts at: 2630 3339 3633
Size: 709 294
Fragments arranged by size:
1043 831 709 326 294 159 99 78
66 28
PvuII CAG'CTG
Cuts at: 0 1152 1320 1953 2229 2262 2397 2625 3549
Size: 1152 168 633 276 33 135 228 924
Cuts at: 3549 3633
Size: 84
Fragments arranged by size:
1152 924 633 276 228 168 135 84
33
SacI G_AGCT'C
Cuts at: 0 2862 2903 3566 3633
Size: 2862 41 663 67
Fragments arranged by size:
2862 663 67 41
SanDI GG'GwC_CC
Cuts at: 0 2776 3633
Size: 2776 857
SapI GCTCTTCn'nnn_
Cuts at: 0 1124 3633
Size: 1124 2509
ScaI AGT'ACT
Cuts at: 0 2034 3633
Size: 2034 1599
SexAI A'CCwGG_T
Cuts at: 0 2173 3633
Size: 2173 1460
SfcI C'TryA_G
Cuts at: 0 444 626 827 1248 1288 1324 1870 2196
Size: 444 182 201 421 40 36 546 326
Cuts at: 2196 2224 2302 2461 2527 2626 3335 3633
Size: 28 78 159 66 99 709 298
Fragments arranged by size:
709 546 444 421 326 298 201 182
159 99 78 66 40 36 28
SfoI GGC'GCC
Cuts at: 0 1405 3591 3633
Size: 1405 2186 42
Fragments arranged by size:
2186 1405 42
SmlI C'TyrA_G
Cuts at: 0 1660 2821 3352 3364 3633
Size: 1660 1161 531 12 269
Fragments arranged by size:
1660 1161 531 269 12
SphI G_CATG'C
Cuts at: 0 1234 3633
Size: 1234 2399
StuI AGG'CCT
Cuts at: 0 568 874 985 2545 2611 2976 3633
Size: 568 306 111 1560 66 365 657
Fragments arranged by size:
1560 657 568 365 306 111 66
StyI C'CwwG_G
Cuts at: 0 1695 1991 2060 3000 3229 3633
Size: 1695 296 69 940 229 404
Fragments arranged by size:
1695 940 404 296 229 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1869 2356 2766 3633
Size: 1869 487 410 867
Fragments arranged by size:
1869 867 487 410
TatI w'GTAC_w
Cuts at: 0 458 521 2032 2496 3633
Size: 458 63 1511 464 1137
Fragments arranged by size:
1511 1137 464 458 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 408 440 1603 1635 1674 1706 3633
Size: 408 32 1163 32 39 32 1927
Fragments arranged by size:
1927 1163 408 39 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 138 3633
Size: 138 3495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1998 3633
Size: 1998 1635
XmnI GAAnn'nnTTC
Cuts at: 0 370 1669 3633
Size: 370 1299 1964
Fragments arranged by size:
1964 1299 370
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApoI AscI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HindIII KasI
MmeI MscI MslI MspA1I NarI NcoI NdeI NspI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI ScaI SexAI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI ApaLI AseI
AsiSI BaeI BciVI BclI BfrBI BglII BmtI BsaBI
BsiEI BsiWI BsmI BsmBI BstEII ClaI CspCI DraI
DrdI EagI EcoRV FalI FseI FspAI HincII HpaI
KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmaI ZraI