(Circular) (Six-base) MAPSORT of: pFN21AA0536.seq Check: 1834 from: 1 to: 7726 pFN21AA0536 7726 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5536 278 Size: 53 83 186 936 4000 2468 Fragments arranged by size: 4000 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3271 3478 5069 1937 Size: 1334 207 1591 4594 Fragments arranged by size: 4594 1591 1334 207 Acc65I G'GTAC_C Cuts at: 5047 5047 Size: 7726 AclI AA'CG_TT Cuts at: 1563 4060 5853 6226 1563 Size: 2497 1793 373 3063 Fragments arranged by size: 3063 2497 1793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2457 3552 3693 4249 4718 4923 5789 7173 Size: 825 1095 141 556 469 205 866 1384 Cuts at: 7173 7441 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1095 866 825 556 469 268 205 141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7529 AflIII A'CryG_T Cuts at: 1203 3684 4522 6625 7602 1203 Size: 2481 838 2103 977 1327 Fragments arranged by size: 2481 2103 1327 977 838 AgeI A'CCGG_T Cuts at: 6557 6557 Size: 7726 AhdI GACnn_n'nnGTC Cuts at: 2920 6455 2920 Size: 3535 4191 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7726 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7726 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3343 3375 3343 Size: 32 7694 AlwNI CAG_nnn'CTG Cuts at: 3911 4954 5469 7041 3911 Size: 1043 515 1572 4596 Fragments arranged by size: 4596 1572 1043 515 ApaLI G'TGCA_C Cuts at: 5783 6939 5783 Size: 1156 6570 ApoI r'AATT_y Cuts at: 1493 2451 3512 4244 4662 5035 5261 5413 7522 Size: 958 1061 732 418 373 226 152 2109 Cuts at: 7522 1493 Size: 1697 Fragments arranged by size: 2109 1697 1061 958 732 418 373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4851 6280 160 Size: 872 3819 1429 1606 Fragments arranged by size: 3819 1606 1429 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7726 AvaI C'yCGr_G Cuts at: 1870 1943 3252 5051 1870 Size: 73 1309 1799 4545 Fragments arranged by size: 4545 1799 1309 73 BamHI G'GATC_C Cuts at: 5056 5423 5056 Size: 367 7359 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3889 4887 5047 6502 618 Size: 325 217 627 2102 998 160 1455 1842 Fragments arranged by size: 2102 1842 1455 998 627 325 217 160 BanII G_rGCy'C Cuts at: 1391 2409 4176 5045 1391 Size: 1018 1767 869 4072 Fragments arranged by size: 4072 1767 1018 869 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2109 2199 2331 3043 3585 3941 4355 Size: 971 210 90 132 712 542 356 414 Cuts at: 4355 4911 928 Size: 556 3743 Fragments arranged by size: 3743 971 712 556 542 414 356 210 132 90 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5917 5951 1903 Size: 34 3980 34 3678 Fragments arranged by size: 3980 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5618 6828 5618 Size: 1210 6516 BclI T'GATC_A Cuts at: 1548 4774 1548 Size: 3226 4500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6337 136 Size: 107 122 71 631 605 4665 1525 Fragments arranged by size: 4665 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2492 2771 3128 3335 3401 7721 2492 Size: 279 357 207 66 4320 2497 Fragments arranged by size: 4320 2497 357 279 207 66 BlpI GC'TnA_GC Cuts at: 5143 5143 Size: 7726 Bme1580I G_kGCm'C Cuts at: 1790 5787 6943 1790 Size: 3997 1156 2573 Fragments arranged by size: 3997 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3275 1318 Size: 1957 5769 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2017 2882 4092 Size: 416 53 83 399 24 600 865 1210 Cuts at: 4092 5457 6415 442 Size: 1365 958 1753 Fragments arranged by size: 1753 1365 1210 958 865 600 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7726 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 7726 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2268 3729 3779 6386 1239 Size: 1029 1461 50 2607 2579 Fragments arranged by size: 2607 2579 1461 1029 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2685 2733 5851 6716 7014 2685 Size: 48 3118 865 298 3397 Fragments arranged by size: 3397 3118 865 298 48 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2012 3111 3318 3360 3387 3462 6389 Size: 617 513 1099 207 42 27 75 2927 Cuts at: 6389 882 Size: 2219 Fragments arranged by size: 2927 2219 1099 617 513 207 75 42 27 BsaAI yAC'GTr Cuts at: 493 1204 3083 5517 493 Size: 711 1879 2434 2702 Fragments arranged by size: 2702 2434 1879 711 BsaBI GATnn'nnATC Cuts at: 3739 4230 4779 7720 3739 Size: 491 549 2941 3745 Fragments arranged by size: 3745 2941 549 491 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5533 5915 6550 275 Size: 53 83 186 936 4000 382 635 1451 Fragments arranged by size: 4000 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6159 6557 6831 6978 7389 6159 Size: 398 274 147 411 6496 Fragments arranged by size: 6496 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 3148 3178 3343 Size: 30 165 30 606 30 1109 30 165 Cuts at: 3343 3373 1178 Size: 30 5531 Fragments arranged by size: 5531 1109 606 165 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3235 3396 3445 1186 Size: 2049 161 49 5467 Fragments arranged by size: 5467 2049 161 49 BseYI C'CCAG_C Cuts at: 3029 4966 6929 7425 3029 Size: 1937 1963 496 3330 Fragments arranged by size: 3330 1963 1937 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2866 5176 5937 6965 1540 Size: 275 186 865 2310 761 1028 2301 Fragments arranged by size: 2310 2301 1028 865 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3946 4176 4576 4847 5787 5872 6943 3946 Size: 230 400 271 940 85 1071 4729 Fragments arranged by size: 4729 1071 940 400 271 230 85 BsmI GAATG_Cn' Cuts at: 5237 5330 5237 Size: 93 7633 BsmBI CGTCTCn'nnnn_ Cuts at: 3217 3217 Size: 7726 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 3946 4176 4576 4847 5045 5787 Size: 399 619 1537 230 400 271 198 742 Cuts at: 5787 5872 6943 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1537 1071 742 619 400 399 271 230 198 85 BspEI T'CCGG_A Cuts at: 7389 7389 Size: 7726 BspHI T'CATG_A Cuts at: 2455 5615 7345 2455 Size: 3160 1730 2836 Fragments arranged by size: 3160 2836 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3093 3369 5081 844 Size: 2249 276 1712 3489 Fragments arranged by size: 3489 2249 1712 276 BsrBI CCG'CTC Cuts at: 1394 1823 5613 1394 Size: 429 3790 3507 Fragments arranged by size: 3790 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4713 6221 6395 65 Size: 1183 3465 1508 174 1396 Fragments arranged by size: 3465 1508 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6370 6557 1700 Size: 110 4560 187 2869 Fragments arranged by size: 4560 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4794 96 Size: 4698 3028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7726 BssSI C'ACGA_G Cuts at: 5786 6798 5786 Size: 1012 6714 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7726 BstBI TT'CG_AA Cuts at: 4744 6530 4744 Size: 1786 5940 BstEII G'GTnAC_C Cuts at: 1184 3024 1184 Size: 1840 5886 BstYI r'GATC_y Cuts at: 1972 2492 2564 2771 2882 3128 3335 3401 4382 Size: 520 72 207 111 246 207 66 981 Cuts at: 4382 4711 5056 5423 5808 5825 7277 7363 7375 Size: 329 345 367 385 17 1452 86 12 Cuts at: 7375 7721 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 981 520 385 367 346 345 329 246 207 207 111 86 72 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 6226 Fragments arranged by size: 6226 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6987 Fragments arranged by size: 6987 683 56 BtsI GCAGTG_nn' Cuts at: 5242 6036 6056 5242 Size: 794 20 6912 Fragments arranged by size: 6912 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7726 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7691 DraI TTT'AAA Cuts at: 3517 4194 4494 5030 3517 Size: 677 300 536 6213 Fragments arranged by size: 6213 677 536 300 DraIII CAC_nnn'GTG Cuts at: 3470 3470 Size: 7726 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6733 809 Size: 507 5417 1802 Fragments arranged by size: 5417 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 5173 6062 Size: 54 1425 50 275 18 177 3166 889 Cuts at: 6062 7691 8 Size: 1629 43 Fragments arranged by size: 3166 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5173 1537 Size: 275 3361 4090 Fragments arranged by size: 4090 3361 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 3169 4268 5656 1353 Size: 1083 733 1099 1388 3423 Fragments arranged by size: 3423 1388 1099 1083 733 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6313 6687 6833 387 Size: 1064 138 4724 374 146 1280 Fragments arranged by size: 4724 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4174 4174 Size: 7726 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2268 2457 3552 3693 3729 3779 4249 Size: 393 636 189 1095 141 36 50 470 Cuts at: 4249 4718 4923 5789 6386 7173 7441 1239 Size: 469 205 866 597 787 268 1524 Fragments arranged by size: 1524 1095 866 787 636 597 470 469 393 268 205 189 141 50 36 EcoO109I rG'GnC_Cy Cuts at: 4864 4993 4864 Size: 129 7597 EcoRI G'AATT_C Cuts at: 5035 5413 5035 Size: 378 7348 EcoRV GAT'ATC Cuts at: 3262 4450 3262 Size: 1188 6538 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3542 3542 Size: 7726 FspI TGC'GCA Cuts at: 6232 6232 Size: 7726 HaeII r_GCGC'y Cuts at: 1678 6873 1678 Size: 5195 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2264 2296 2906 Size: 32 135 32 437 32 223 32 610 Cuts at: 2906 2927 2938 2959 2990 3022 3113 3145 3148 Size: 21 11 21 31 32 91 32 3 Cuts at: 3148 3180 3246 3278 3539 3571 4419 4451 4496 Size: 32 66 32 261 32 848 32 45 Cuts at: 4496 4528 4607 4639 6367 6399 6441 6473 1373 Size: 32 79 32 1728 32 42 32 2626 Fragments arranged by size: 2626 1728 848 610 437 261 223 135 91 79 66 45 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 21 21 11 3 HincII GTy'rAC Cuts at: 669 1657 1938 5070 5316 669 Size: 988 281 3132 246 3079 Fragments arranged by size: 3132 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4496 4700 748 Size: 3748 204 3774 Fragments arranged by size: 3774 3748 204 HpaI GTT'AAC Cuts at: 5316 5316 Size: 7726 KpnI G_GTAC'C Cuts at: 5051 5051 Size: 7726 MfeI C'AATT_G Cuts at: 3645 5325 3645 Size: 1680 6046 MluI A'CGCG_T Cuts at: 7602 7602 Size: 7726 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3341 3407 4880 4923 4941 6840 7024 1305 Size: 2036 66 1473 43 18 1899 184 2007 Fragments arranged by size: 2036 2007 1899 1473 184 66 43 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5904 Fragments arranged by size: 5904 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 5684 6043 6202 518 Size: 648 2523 1995 359 159 2042 Fragments arranged by size: 2523 2042 1995 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 3959 5142 5819 6967 7212 1606 Size: 409 1944 1183 677 1148 245 2120 Fragments arranged by size: 2120 1944 1183 1148 677 409 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6235 Fragments arranged by size: 6235 939 552 NdeI CA'TA_TG Cuts at: 387 4692 387 Size: 4305 3421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7726 NotI GC'GGCC_GC Cuts at: 5173 5173 Size: 7726 NruI TCG'CGA Cuts at: 2862 2862 Size: 7726 NspI r_CATG'y Cuts at: 1132 1229 3688 4445 4526 5084 6629 1132 Size: 97 2459 757 81 558 1545 2229 Fragments arranged by size: 2459 2229 1545 757 558 97 81 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7726 PciI A'CATG_T Cuts at: 3684 4522 6625 3684 Size: 838 2103 4785 Fragments arranged by size: 4785 2103 838 PflMI CCAn_nnn'nTGG Cuts at: 1108 4144 1108 Size: 3036 4690 PfoI T'CCnGG_A Cuts at: 5013 5013 Size: 7726 PmeI GTTT'AAAC Cuts at: 5030 5030 Size: 7726 PmlI CAC'GTG Cuts at: 3083 3083 Size: 7726 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3235 3267 3343 3375 5795 5827 7308 7340 3235 Size: 32 76 32 2420 32 1481 32 3621 Fragments arranged by size: 3621 2420 1481 76 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4864 4864 Size: 7726 PshAI GACnn'nnGTC Cuts at: 1613 2587 3423 1613 Size: 974 836 5916 Fragments arranged by size: 5916 974 836 PsiI TTA'TAA Cuts at: 2843 5296 2843 Size: 2453 5273 PstI C_TGCA'G Cuts at: 830 5078 830 Size: 4248 3478 PvuI CG_AT'CG Cuts at: 2001 2866 2001 Size: 865 6861 SacI G_AGCT'C Cuts at: 4176 4176 Size: 7726 SacII CC_GC'GG Cuts at: 2016 2016 Size: 7726 SalI G'TCGA_C Cuts at: 1936 5068 1936 Size: 3132 4594 SapI GCTCTTCn'nnn_ Cuts at: 3169 3169 Size: 7726 SbfI CC_TGCA'GG Cuts at: 5078 5078 Size: 7726 ScaI AGT'ACT Cuts at: 2488 5974 7686 2488 Size: 3486 1712 2528 Fragments arranged by size: 3486 2528 1712 SexAI A'CCwGG_T Cuts at: 3040 3040 Size: 7726 SfcI C'TryA_G Cuts at: 826 1045 4387 4626 4997 5074 6209 6890 7081 Size: 219 3342 239 371 77 1135 681 191 Cuts at: 7081 826 Size: 1471 Fragments arranged by size: 3342 1471 1135 681 371 239 219 191 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7726 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7726 SmaI CCC'GGG Cuts at: 1872 5053 1872 Size: 3181 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2700 2748 3252 5830 6731 6993 Size: 197 926 757 48 504 2578 901 262 Cuts at: 6993 820 Size: 1553 Fragments arranged by size: 2578 1553 926 901 757 504 262 197 48 SnaBI TAC'GTA Cuts at: 493 5517 493 Size: 5024 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7726 SphI G_CATG'C Cuts at: 1132 5084 1132 Size: 3952 3774 SspI AAT'ATT Cuts at: 5 52 5650 5 Size: 47 5598 2081 Fragments arranged by size: 5598 2081 47 StuI AGG'CCT Cuts at: 1517 3818 1517 Size: 2301 5425 StyI C'CwwG_G Cuts at: 513 1065 2004 5165 513 Size: 552 939 3161 3074 Fragments arranged by size: 3161 3074 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3043 4025 4875 5753 5770 5923 6108 Size: 1255 1463 982 850 878 17 153 185 Cuts at: 6108 6553 325 Size: 445 1498 Fragments arranged by size: 1498 1463 1255 982 878 850 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2486 2501 4794 Size: 274 80 33 51 1443 509 15 2293 Cuts at: 4794 4921 5972 7684 96 Size: 127 1051 1712 138 Fragments arranged by size: 2293 1712 1443 1051 509 274 138 127 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4837 4869 7686 7718 4837 Size: 32 2817 32 4845 Fragments arranged by size: 4845 2817 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2023 3042 3160 1316 Size: 707 1019 118 5882 Fragments arranged by size: 5882 1019 707 118 XbaI T'CTAG_A Cuts at: 5062 5062 Size: 7726 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4141 4141 Size: 7726 XhoI C'TCGA_G Cuts at: 1943 3252 1943 Size: 1309 6417 XmaI C'CCGG_G Cuts at: 1870 5051 1870 Size: 3181 4545 XmnI GAAnn'nnTTC Cuts at: 3526 5855 3526 Size: 2329 5397 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5534 276 Size: 53 83 186 936 4000 2468 Fragments arranged by size: 4000 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PspOMI PspXI PsrI PvuII RsrII SanDI SfiI SfoI SrfI SwaI