Restriction map for
pFN21AA0536
(Linear) (Six-base) MAPSORT of: KIAA0536.seq Check: 3347 from: 1 to: 3021
KIAA0536.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AccI GT'mk_AC
Cuts at: 0 1266 1473 3021
Size: 1266 207 1548
Fragments arranged by size:
1548 1266 207
AclI AA'CG_TT
Cuts at: 0 2055 3021
Size: 2055 966
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1547 1688 2244 2713 2918 3021
Size: 452 1095 141 556 469 205 103
Fragments arranged by size:
1095 556 469 452 205 141 103
AflIII A'CryG_T
Cuts at: 0 1679 2517 3021
Size: 1679 838 504
Fragments arranged by size:
1679 838 504
AhdI GACnn_n'nnGTC
Cuts at: 0 915 3021
Size: 915 2106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1338 1370 3021
Size: 1338 32 1651
Fragments arranged by size:
1651 1338 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1906 2949 3021
Size: 1906 1043 72
Fragments arranged by size:
1906 1043 72
ApoI r'AATT_y
Cuts at: 0 446 1507 2239 2657 3021
Size: 446 1061 732 418 364
Fragments arranged by size:
1061 732 446 418 364
AseI AT'TA_AT
Cuts at: 0 2846 3021
Size: 2846 175
AvaI C'yCGr_G
Cuts at: 0 1247 3021
Size: 1247 1774
BanI G'GyrC_C
Cuts at: 0 1884 2882 3021
Size: 1884 998 139
Fragments arranged by size:
1884 998 139
BanII G_rGCy'C
Cuts at: 0 404 2171 3021
Size: 404 1767 850
Fragments arranged by size:
1767 850 404
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 194 326 1038 1580 1936 2350 2906
Size: 104 90 132 712 542 356 414 556
Cuts at: 2906 3021
Size: 115
Fragments arranged by size:
712 556 542 414 356 132 115 104
90
BclI T'GATC_A
Cuts at: 0 2769 3021
Size: 2769 252
BglII A'GATC_T
Cuts at: 0 487 766 1123 1330 1396 3021
Size: 487 279 357 207 66 1625
Fragments arranged by size:
1625 487 357 279 207 66
BmgBI CAC'GTC
Cuts at: 0 1270 3021
Size: 1270 1751
BmrI ACTGGGnnnn_n'
Cuts at: 0 12 877 2087 3021
Size: 12 865 1210 934
Fragments arranged by size:
1210 934 865 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 444 3021
Size: 444 2577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1724 1774 3021
Size: 263 1461 50 1247
Fragments arranged by size:
1461 1247 263 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 728 3021
Size: 680 48 2293
Fragments arranged by size:
2293 680 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 1106 1313 1355 1382 1457 3021
Size: 7 1099 207 42 27 75 1564
Fragments arranged by size:
1564 1099 207 75 42 27 7
BsaAI yAC'GTr
Cuts at: 0 1078 3021
Size: 1078 1943
BsaBI GATnn'nnATC
Cuts at: 0 1734 2225 2774 3021
Size: 1734 491 549 247
Fragments arranged by size:
1734 549 491 247
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 1143 1173 1338 1368 3021
Size: 4 30 1109 30 165 30 1653
Fragments arranged by size:
1653 1109 165 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1230 1391 1440 3021
Size: 1230 161 49 1581
Fragments arranged by size:
1581 1230 161 49
BseYI C'CCAG_C
Cuts at: 0 1024 2961 3021
Size: 1024 1937 60
Fragments arranged by size:
1937 1024 60
BsiEI CG_ry'CG
Cuts at: 0 861 3021
Size: 861 2160
BsiHKAI G_wGCw'C
Cuts at: 0 1941 2171 2571 2842 3021
Size: 1941 230 400 271 179
Fragments arranged by size:
1941 400 271 230 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1212 3021
Size: 1212 1809
Bsp1286I G_dGCh'C
Cuts at: 0 404 1941 2171 2571 2842 3021
Size: 404 1537 230 400 271 179
Fragments arranged by size:
1537 404 400 271 230 179
BspHI T'CATG_A
Cuts at: 0 450 3021
Size: 450 2571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1088 1364 3021
Size: 1088 276 1657
Fragments arranged by size:
1657 1088 276
BsrDI GCAATG_nn'
Cuts at: 0 2708 3021
Size: 2708 313
BsrGI T'GTAC_A
Cuts at: 0 2789 3021
Size: 2789 232
BstBI TT'CG_AA
Cuts at: 0 2739 3021
Size: 2739 282
BstEII G'GTnAC_C
Cuts at: 0 1019 3021
Size: 1019 2002
BstYI r'GATC_y
Cuts at: 0 487 559 766 877 1123 1330 1396 2377
Size: 487 72 207 111 246 207 66 981
Cuts at: 2377 2706 3021
Size: 329 315
Fragments arranged by size:
981 487 329 315 246 207 207 111
72 66
BtgI C'CryG_G
Cuts at: 0 8 3021
Size: 8 3013
DraI TTT'AAA
Cuts at: 0 1512 2189 2489 3021
Size: 1512 677 300 532
Fragments arranged by size:
1512 677 532 300
DraIII CAC_nnn'GTG
Cuts at: 0 1465 3021
Size: 1465 1556
EaeI y'GGCC_r
Cuts at: 0 2 3021
Size: 2 3019
EarI CTCTTCn'nnn_
Cuts at: 0 431 1164 2263 3021
Size: 431 733 1099 758
Fragments arranged by size:
1099 758 733 431
EcoICRI GAG'CTC
Cuts at: 0 2169 3021
Size: 2169 852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 452 1547 1688 1724 1774 2244 2713
Size: 263 189 1095 141 36 50 470 469
Cuts at: 2713 2918 3021
Size: 205 103
Fragments arranged by size:
1095 470 469 263 205 189 141 103
50 36
EcoO109I rG'GnC_Cy
Cuts at: 0 2859 2988 3021
Size: 2859 129 33
Fragments arranged by size:
2859 129 33
EcoRV GAT'ATC
Cuts at: 0 1257 2445 3021
Size: 1257 1188 576
Fragments arranged by size:
1257 1188 576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1537 3021
Size: 1537 1484
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 259 291 901 922 933 954
Size: 4 32 223 32 610 21 11 21
Cuts at: 954 985 1017 1108 1140 1143 1175 1241 1273
Size: 31 32 91 32 3 32 66 32
Cuts at: 1273 1534 1566 2414 2446 2491 2523 2602 2634
Size: 261 32 848 32 45 32 79 32
Cuts at: 2634 3021
Size: 387
Fragments arranged by size:
848 610 387 261 223 91 79 66
45 32 32 32 32 32 32 32
32 32 32 31 21 21 11 4
3
HindIII A'AGCT_T
Cuts at: 0 2491 2695 3021
Size: 2491 204 326
Fragments arranged by size:
2491 326 204
MfeI C'AATT_G
Cuts at: 0 1640 3021
Size: 1640 1381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1402 2875 2918 2936 3021
Size: 1336 66 1473 43 18 85
Fragments arranged by size:
1473 1336 85 66 43 18
MslI CAynn'nnrTG
Cuts at: 0 1684 3021
Size: 1684 1337
MspA1I CmG'CkG
Cuts at: 0 10 1954 3021
Size: 10 1944 1067
Fragments arranged by size:
1944 1067 10
NdeI CA'TA_TG
Cuts at: 0 2687 3021
Size: 2687 334
NruI TCG'CGA
Cuts at: 0 857 3021
Size: 857 2164
NspI r_CATG'y
Cuts at: 0 1683 2440 2521 3021
Size: 1683 757 81 500
Fragments arranged by size:
1683 757 500 81
PciI A'CATG_T
Cuts at: 0 1679 2517 3021
Size: 1679 838 504
Fragments arranged by size:
1679 838 504
PflMI CCAn_nnn'nTGG
Cuts at: 0 2139 3021
Size: 2139 882
PfoI T'CCnGG_A
Cuts at: 0 3008 3021
Size: 3008 13
PmlI CAC'GTG
Cuts at: 0 1078 3021
Size: 1078 1943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1230 1262 1338 1370 3021
Size: 1230 32 76 32 1651
Fragments arranged by size:
1651 1230 76 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2859 3021
Size: 2859 162
PshAI GACnn'nnGTC
Cuts at: 0 582 1418 3021
Size: 582 836 1603
Fragments arranged by size:
1603 836 582
PsiI TTA'TAA
Cuts at: 0 838 3021
Size: 838 2183
PvuI CG_AT'CG
Cuts at: 0 861 3021
Size: 861 2160
SacI G_AGCT'C
Cuts at: 0 2171 3021
Size: 2171 850
SacII CC_GC'GG
Cuts at: 0 11 3021
Size: 11 3010
SapI GCTCTTCn'nnn_
Cuts at: 0 1164 3021
Size: 1164 1857
ScaI AGT'ACT
Cuts at: 0 483 3021
Size: 483 2538
SexAI A'CCwGG_T
Cuts at: 0 1035 3021
Size: 1035 1986
SfcI C'TryA_G
Cuts at: 0 2382 2621 2992 3021
Size: 2382 239 371 29
Fragments arranged by size:
2382 371 239 29
SmlI C'TyrA_G
Cuts at: 0 695 743 1247 3021
Size: 695 48 504 1774
Fragments arranged by size:
1774 695 504 48
StuI AGG'CCT
Cuts at: 0 1813 3021
Size: 1813 1208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1038 2020 2870 3021
Size: 1038 982 850 151
Fragments arranged by size:
1038 982 850 151
TatI w'GTAC_w
Cuts at: 0 481 496 2789 2916 3021
Size: 481 15 2293 127 105
Fragments arranged by size:
2293 481 127 105 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2832 2864 3021
Size: 2832 32 157
Fragments arranged by size:
2832 157 32
Tth111I GACn'n_nGTC
Cuts at: 0 18 1037 1155 3021
Size: 18 1019 118 1866
Fragments arranged by size:
1866 1019 118 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2136 3021
Size: 2136 885
XhoI C'TCGA_G
Cuts at: 0 1247 3021
Size: 1247 1774
XmnI GAAnn'nnTTC
Cuts at: 0 1521 3021
Size: 1521 1500
Enzymes that do cut:
AccI AclI AcuI AflIII AhdI AloI AlwNI ApoI
AseI AvaI BanI BanII BbsI BclI BglII BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrGI BstBI BstEII BstYI BtgI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I
HindIII MfeI MmeI MslI MspA1I NdeI NruI NspI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI
PvuI SacI SacII SapI ScaI SexAI SfcI SmlI
StuI TaqII TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AleI AlfI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmtI
Bpu10I BsaHI BsaWI BsgI BsiWI BsmI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DrdI EagI EciI EcoNI EcoRI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NsiI PacI PasI PmeI PspOMI PspXI PsrI PstI
PvuII RsrII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI XbaI XmaI ZraI