(Circular) (Six-base) MAPSORT of: pFN21AA0384.seq Check: 3096 from: 1 to: 7522 pFN21AA0384 7522 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5332 278 Size: 53 83 186 936 3796 2468 Fragments arranged by size: 3796 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2027 2761 4865 1937 Size: 90 734 2104 4594 Fragments arranged by size: 4594 2104 734 90 Acc65I G'GTAC_C Cuts at: 4843 4843 Size: 7522 AclI AA'CG_TT Cuts at: 1563 5649 6022 1563 Size: 4086 373 3063 Fragments arranged by size: 4086 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3432 5585 6969 7237 1632 Size: 1800 2153 1384 268 1917 Fragments arranged by size: 2153 1917 1800 1384 268 AflII C'TTAA_G Cuts at: 820 1017 4019 820 Size: 197 3002 4323 Fragments arranged by size: 4323 3002 197 AflIII A'CryG_T Cuts at: 1203 2131 6421 7398 1203 Size: 928 4290 977 1327 Fragments arranged by size: 4290 1327 977 928 AgeI A'CCGG_T Cuts at: 6353 6353 Size: 7522 AhdI GACnn_n'nnGTC Cuts at: 2538 4098 6251 2538 Size: 1560 2153 3809 Fragments arranged by size: 3809 2153 1560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7522 AlwNI CAG_nnn'CTG Cuts at: 5265 6837 5265 Size: 1572 5950 ApaI G_GGCC'C Cuts at: 2781 2781 Size: 7522 ApaLI G'TGCA_C Cuts at: 3275 5579 6735 3275 Size: 2304 1156 4062 Fragments arranged by size: 4062 2304 1156 ApoI r'AATT_y Cuts at: 1493 2240 4309 4665 4831 5057 5209 7318 1493 Size: 747 2069 356 166 226 152 2109 1697 Fragments arranged by size: 2109 2069 1697 747 356 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 4241 6076 160 Size: 872 3209 1835 1606 Fragments arranged by size: 3209 1835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7522 AvaI C'yCGr_G Cuts at: 1870 1943 2085 2700 3963 4847 1870 Size: 73 142 615 1263 884 4545 Fragments arranged by size: 4545 1263 884 615 142 73 AvrII C'CTAG_G Cuts at: 2612 2612 Size: 7522 BamHI G'GATC_C Cuts at: 2350 3916 4852 5219 2350 Size: 1566 936 367 4653 Fragments arranged by size: 4653 1566 936 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2752 2952 2989 4843 Size: 325 217 627 319 646 200 37 1854 Cuts at: 4843 6298 618 Size: 1455 1842 Fragments arranged by size: 1854 1842 1455 646 627 325 319 217 200 37 BanII G_rGCy'C Cuts at: 1391 2781 3016 3298 3978 4841 1391 Size: 1390 235 282 680 863 4072 Fragments arranged by size: 4072 1390 863 680 282 235 BbeI G_GCGC'C Cuts at: 2110 2956 2110 Size: 846 6676 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2447 2976 3212 3552 928 Size: 971 548 529 236 340 4898 Fragments arranged by size: 4898 971 548 529 340 236 BbvCI CC'TCA_GC Cuts at: 4054 4054 Size: 7522 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5713 5747 1903 Size: 34 3776 34 3678 Fragments arranged by size: 3776 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5414 6624 5414 Size: 1210 6312 BclI T'GATC_A Cuts at: 1548 3507 4425 1548 Size: 1959 918 4645 Fragments arranged by size: 4645 1959 918 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2845 6133 136 Size: 107 122 71 631 605 1173 3288 1525 Fragments arranged by size: 3288 1525 1173 631 605 122 107 71 BglII A'GATC_T Cuts at: 7517 7517 Size: 7522 BlpI GC'TnA_GC Cuts at: 4939 4939 Size: 7522 Bme1580I G_kGCm'C Cuts at: 1790 2755 2781 3279 5583 6739 1790 Size: 965 26 498 2304 1156 2573 Fragments arranged by size: 2573 2304 1156 965 498 26 BmgBI CAC'GTC Cuts at: 1318 2113 3214 3320 1318 Size: 795 1101 106 5520 Fragments arranged by size: 5520 1101 795 106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2062 2937 3019 Size: 416 53 83 399 24 645 875 82 Cuts at: 3019 3230 3846 5253 6211 442 Size: 211 616 1407 958 1753 Fragments arranged by size: 1753 1407 958 875 645 616 416 399 211 83 82 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7522 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3651 3907 3978 4050 3651 Size: 256 71 72 7123 Fragments arranged by size: 7123 256 72 71 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 3877 4080 4218 6182 1239 Size: 875 1763 203 138 1964 2579 Fragments arranged by size: 2579 1964 1763 875 203 138 Bpu10I CC'TnA_GC Cuts at: 4054 4054 Size: 7522 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3209 3284 5647 6512 6810 3209 Size: 75 2363 865 298 3921 Fragments arranged by size: 3921 2363 865 298 75 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2146 2327 2378 2411 4731 6185 882 Size: 617 647 181 51 33 2320 1454 2219 Fragments arranged by size: 2320 2219 1454 647 617 181 51 33 BsaAI yAC'GTr Cuts at: 493 1204 5313 493 Size: 711 4109 2702 Fragments arranged by size: 4109 2702 711 BsaBI GATnn'nnATC Cuts at: 2664 2820 4007 7516 2664 Size: 156 1187 3509 2670 Fragments arranged by size: 3509 2670 1187 156 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2107 2953 5329 5711 Size: 53 83 186 936 574 846 2376 382 Cuts at: 5711 6346 275 Size: 635 1451 Fragments arranged by size: 2376 1451 936 846 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3422 4626 5955 6353 6627 6774 7185 3422 Size: 1204 1329 398 274 147 411 3759 Fragments arranged by size: 3759 1329 1204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7297 Fragments arranged by size: 7297 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2360 2732 2999 3640 4291 4749 1186 Size: 925 249 372 267 641 651 458 3959 Fragments arranged by size: 3959 925 651 641 458 372 267 249 BseYI C'CCAG_C Cuts at: 2732 3024 4082 6725 7221 2732 Size: 292 1058 2643 496 3033 Fragments arranged by size: 3033 2643 1058 496 292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6949 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 2206 4763 4972 5733 6761 Size: 275 186 30 175 2557 209 761 1028 Cuts at: 6761 1540 Size: 2301 Fragments arranged by size: 2557 2301 1028 761 275 209 186 175 30 BsiHKAI G_wGCw'C Cuts at: 3279 3298 3585 3978 4204 5583 5668 6739 3279 Size: 19 287 393 226 1379 85 1071 4062 Fragments arranged by size: 4062 1379 1071 393 287 226 85 19 BsmI GAATG_Cn' Cuts at: 5033 5126 5033 Size: 93 7429 BsmBI CGTCTCn'nnnn_ Cuts at: 2119 2119 Size: 7522 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2755 2781 3016 3279 3298 3585 3978 Size: 399 965 26 235 263 19 287 393 Cuts at: 3978 4204 4841 5583 5668 6739 1391 Size: 226 637 742 85 1071 2174 Fragments arranged by size: 2174 1071 965 742 637 399 393 287 263 235 226 85 26 19 BspEI T'CCGG_A Cuts at: 7185 7185 Size: 7522 BspHI T'CATG_A Cuts at: 2958 5411 7141 2958 Size: 2453 1730 3339 Fragments arranged by size: 3339 2453 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3180 4877 844 Size: 2336 1697 3489 Fragments arranged by size: 3489 2336 1697 BsrBI CCG'CTC Cuts at: 1394 1823 2722 3532 4113 4446 5409 1394 Size: 429 899 810 581 333 963 3507 Fragments arranged by size: 3507 963 899 810 581 429 333 BsrDI GCAATG_nn' Cuts at: 65 1248 2569 3199 6017 6191 65 Size: 1183 1321 630 2818 174 1396 Fragments arranged by size: 2818 1396 1321 1183 630 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2190 2205 6166 6353 1700 Size: 110 380 15 3961 187 2869 Fragments arranged by size: 3961 2869 380 187 110 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7522 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7522 BssSI C'ACGA_G Cuts at: 3393 5582 6594 3393 Size: 2189 1012 4321 Fragments arranged by size: 4321 2189 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3488 794 Size: 2694 4828 BstBI TT'CG_AA Cuts at: 3385 6326 3385 Size: 2941 4581 BstEII G'GTnAC_C Cuts at: 1184 2704 1184 Size: 1520 6002 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2041 Size: 7522 BstYI r'GATC_y Cuts at: 1972 2350 2815 3783 3916 4768 4852 5219 5604 Size: 378 465 968 133 852 84 367 385 Cuts at: 5604 5621 7073 7159 7171 7517 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 968 852 465 385 378 367 346 133 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2355 3605 2355 Size: 1250 6272 BtgI C'CryG_G Cuts at: 513 1065 2004 2421 513 Size: 552 939 417 5614 Fragments arranged by size: 5614 939 552 417 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2232 2807 487 Size: 683 56 1006 575 5202 Fragments arranged by size: 5202 1006 683 575 56 BtsI GCAGTG_nn' Cuts at: 2598 2683 2913 3482 5038 5832 5852 2598 Size: 85 230 569 1556 794 20 4268 Fragments arranged by size: 4268 1556 794 569 230 85 20 ClaI AT'CG_AT Cuts at: 1554 4759 1554 Size: 3205 4317 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7487 DraI TTT'AAA Cuts at: 4826 4826 Size: 7522 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6529 809 Size: 507 5213 1802 Fragments arranged by size: 5213 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2203 4969 Size: 54 1425 50 275 18 339 34 2766 Cuts at: 4969 5858 7487 8 Size: 889 1629 43 Fragments arranged by size: 2766 1629 1425 889 339 275 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2203 4969 1537 Size: 275 391 2766 4090 Fragments arranged by size: 4090 2766 391 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5452 1353 Size: 4099 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6109 6483 6629 387 Size: 1064 138 4520 374 146 1280 Fragments arranged by size: 4520 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3296 3976 3296 Size: 680 6842 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 3432 3877 4080 4218 5585 6182 Size: 393 482 1318 445 203 138 1367 597 Cuts at: 6182 6969 7237 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1367 1318 787 597 482 445 393 268 203 138 EcoO109I rG'GnC_Cy Cuts at: 2570 2778 3074 2570 Size: 208 296 7018 Fragments arranged by size: 7018 296 208 EcoRI G'AATT_C Cuts at: 4831 5209 4831 Size: 378 7144 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3296 3296 Size: 7522 FspI TGC'GCA Cuts at: 3065 6028 3065 Size: 2963 4559 HaeII r_GCGC'y Cuts at: 1678 2093 2110 2956 4090 6669 1678 Size: 415 17 846 1134 2579 2531 Fragments arranged by size: 2579 2531 1134 846 415 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2492 2524 2556 Size: 32 135 32 437 32 451 32 32 Cuts at: 2556 2978 3010 3499 3531 3560 3592 4084 4116 Size: 422 32 489 32 29 32 492 32 Cuts at: 4116 6163 6195 6237 6269 1373 Size: 2047 32 42 32 2626 Fragments arranged by size: 2626 2047 492 489 451 437 422 135 42 32 32 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2028 4866 5112 669 Size: 988 281 90 2838 246 3079 Fragments arranged by size: 3079 2838 988 281 246 90 HindIII A'AGCT_T Cuts at: 748 3049 3725 4388 748 Size: 2301 676 663 3882 Fragments arranged by size: 3882 2301 676 663 HpaI GTT'AAC Cuts at: 5112 5112 Size: 7522 KasI G'GCGC_C Cuts at: 2106 2952 2106 Size: 846 6676 KpnI G_GTAC'C Cuts at: 4847 4847 Size: 7522 MfeI C'AATT_G Cuts at: 5121 5121 Size: 7522 MluI A'CGCG_T Cuts at: 2131 7398 2131 Size: 5267 2255 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2903 6636 6820 1305 Size: 1598 3733 184 2007 Fragments arranged by size: 3733 2007 1598 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 10 Size: 54 1425 343 339 5361 Fragments arranged by size: 5361 1425 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 4596 5480 5839 5998 518 Size: 648 3430 884 359 159 2042 Fragments arranged by size: 3430 2042 884 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 3164 3596 4058 4938 5615 6763 7008 Size: 519 1039 432 462 880 677 1148 245 Cuts at: 7008 1606 Size: 2120 Fragments arranged by size: 2120 1148 1039 880 677 519 462 432 245 NaeI GCC'GGC Cuts at: 2192 2192 Size: 7522 NarI GG'CG_CC Cuts at: 2107 2953 2107 Size: 846 6676 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6031 Fragments arranged by size: 6031 939 552 NdeI CA'TA_TG Cuts at: 387 4597 387 Size: 4210 3312 NgoMIV G'CCGG_C Cuts at: 2190 2190 Size: 7522 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7522 NotI GC'GGCC_GC Cuts at: 4969 4969 Size: 7522 NspI r_CATG'y Cuts at: 1132 1229 3491 3848 4256 4880 6425 1132 Size: 97 2262 357 408 624 1545 2229 Fragments arranged by size: 2262 2229 1545 624 408 357 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7522 PciI A'CATG_T Cuts at: 6421 6421 Size: 7522 PflMI CCAn_nnn'nTGG Cuts at: 1108 2536 1108 Size: 1428 6094 PmeI GTTT'AAAC Cuts at: 4826 4826 Size: 7522 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5591 5623 7104 7136 5591 Size: 32 1481 32 5977 Fragments arranged by size: 5977 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2570 2570 Size: 7522 PshAI GACnn'nnGTC Cuts at: 1613 2423 2946 1613 Size: 810 523 6189 Fragments arranged by size: 6189 810 523 PsiI TTA'TAA Cuts at: 5092 5092 Size: 7522 PspOMI G'GGCC_C Cuts at: 2777 2777 Size: 7522 PspXI vC'TCGA_Gb Cuts at: 3963 3963 Size: 7522 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2609 2641 2609 Size: 32 7490 PstI C_TGCA'G Cuts at: 830 4874 830 Size: 4044 3478 PvuI CG_AT'CG Cuts at: 2001 4763 2001 Size: 2762 4760 PvuII CAG'CTG Cuts at: 2125 3164 3596 4058 2125 Size: 1039 432 462 5589 Fragments arranged by size: 5589 1039 462 432 SacI G_AGCT'C Cuts at: 3298 3978 3298 Size: 680 6842 SalI G'TCGA_C Cuts at: 1936 2026 4864 1936 Size: 90 2838 4594 Fragments arranged by size: 4594 2838 90 SbfI CC_TGCA'GG Cuts at: 4874 4874 Size: 7522 ScaI AGT'ACT Cuts at: 3240 4609 5770 7482 3240 Size: 1369 1161 1712 3280 Fragments arranged by size: 3280 1712 1369 1161 SexAI A'CCwGG_T Cuts at: 2572 2572 Size: 7522 SfcI C'TryA_G Cuts at: 826 1045 2370 3909 4870 6005 6686 6877 826 Size: 219 1325 1539 961 1135 681 191 1471 Fragments arranged by size: 1539 1471 1325 1135 961 681 219 191 SfoI GGC'GCC Cuts at: 2108 2954 2108 Size: 846 6676 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7522 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7522 SmaI CCC'GGG Cuts at: 1872 2087 2702 4849 1872 Size: 215 615 2147 4545 Fragments arranged by size: 4545 2147 615 215 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 3299 3963 4019 5626 6527 Size: 197 926 1281 75 664 56 1607 901 Cuts at: 6527 6789 820 Size: 262 1553 Fragments arranged by size: 1607 1553 1281 926 901 664 262 197 75 56 SnaBI TAC'GTA Cuts at: 493 5313 493 Size: 4820 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7522 SphI G_CATG'C Cuts at: 1132 3491 4880 1132 Size: 2359 1389 3774 Fragments arranged by size: 3774 2359 1389 SspI AAT'ATT Cuts at: 5 52 5446 5 Size: 47 5394 2081 Fragments arranged by size: 5394 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7522 StyI C'CwwG_G Cuts at: 513 1065 2004 2612 3280 4961 513 Size: 552 939 608 668 1681 3074 Fragments arranged by size: 3074 1681 939 668 608 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5549 5566 5719 5904 6349 325 Size: 1255 3969 17 153 185 445 1498 Fragments arranged by size: 3969 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 3238 4607 Size: 274 80 33 51 1443 484 777 1369 Cuts at: 4607 5768 7480 96 Size: 1161 1712 138 Fragments arranged by size: 1712 1443 1369 1161 777 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7482 7514 7482 Size: 32 7490 Tth111I GACn'n_nGTC Cuts at: 1316 2574 4325 1316 Size: 1258 1751 4513 Fragments arranged by size: 4513 1751 1258 XbaI T'CTAG_A Cuts at: 4858 4858 Size: 7522 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2164 2657 3087 2164 Size: 493 430 6599 Fragments arranged by size: 6599 493 430 XhoI C'TCGA_G Cuts at: 1943 3963 1943 Size: 2020 5502 XmaI C'CCGG_G Cuts at: 1870 2085 2700 4847 1870 Size: 215 615 2147 4545 Fragments arranged by size: 4545 2147 615 215 XmnI GAAnn'nnTTC Cuts at: 4665 5651 4665 Size: 986 6536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5330 276 Size: 53 83 186 936 3796 2468 Fragments arranged by size: 3796 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI RsrII SacII SanDI SapI SfiI SrfI SwaI