Restriction map for
pFN21AA0366
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AclI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BlnI BsiEI BsiWI
BspDI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FseI HaeII HpaI KasI KspI
MluI MluNI MscI NarI NciI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 3584 GACGTC GACGT^C
Acc65I 2180 GGTACC G^GTACC
AccI 598 GTCTAC GT^[AC][GT]AC
AccIII 407 TCCGGA T^CCGGA
AflII 2185 CTTAAG C^TTAAG
Asp700I 2377 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2180 GGTACC G^GTACC
AvaI 2863 CCCGAG C^[CT]CG[GA]G
AviII 917 TGCGCA TGC^GCA
BclI 1383 TGATCA T^GATCA
BfrI 2185 CTTAAG C^TTAAG
BlpI 3400 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3400 GCTCAGC GC^T[ACGT]AGC
BsaAI 396 TACGTA [CT]AC^GT[GA]
BsaBI 268 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 407 TCCGGA [AT]^CCGG[AT]
BseAI 407 TCCGGA T^CCGGA
BsmBI 735 GAGACG CGTCTC(1/5)
BsoBI 2863 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2710 TGTACA T^GTACA
BspEI 407 TCCGGA T^CCGGA
BsrGI 2710 TGTACA T^GTACA
CelII 3400 GCTCAGC GC^T[ACGT]AGC
DraI 2292 TTTAAA TTT^AAA
DrdI 796 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 813 CCATGG C^C[GA][CT]GG
EcoT22I 3338 ATGCAT ATGCA^T
FbaI 1383 TGATCA T^GATCA
FspI 917 TGCGCA TGC^GCA
HincII 3029 GTCAAC GT[CT]^[GA]AC
HindII 3029 GTCAAC GT[CT]^[GA]AC
KpnI 2180 GGTACC GGTAC^C
MamI 268 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1656 CAATTG C^AATTG
MroI 407 TCCGGA T^CCGGA
MunI 1656 CAATTG C^AATTG
NaeI 2873 GCCGGC GCC^GGC
NcoI 813 CCATGG C^CATGG
NgoMIV 2873 GCCGGC G^CCGGC
NsiI 3338 ATGCAT ATGCA^T
PshAI 135 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2553 GAAGAGC GCTCTTC(1/4)
SexAI 3117 ACCAGGT A^CC[AT]GGT
SnaBI 396 TACGTA TAC^GTA
SpeI 300 ACTAGT A^CTAGT
SspBI 2710 TGTACA T^GTACA
SspI 2698 AATATT AAT^ATT
StuI 675 AGGCCT AGG^CCT
TaqI 773 TCGA T^CGA
TthHB8I 773 TCGA T^CGA
XmnI 2377 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 869 AAATTT [GA]^AATT[CT]
1675 AAATTT
Alw44I 1476 GTGCAC G^TGCAC
2833 GTGCAC
ApaLI 1476 GTGCAC G^TGCAC
2833 GTGCAC
ApoI 869 AAATTT [GA]^AATT[CT]
1675 AAATTT
BsaI 3283 GAGACC GGTCTC(1/5)
2561 GGTCTC
BsgI 2748 CTGCAC GTGCAG(16/14)
2900 CTGCAC
BsrDI 1615 CATTGC GCAATG(2/0)
1440 GCAATG
BsrFI 1117 ACCGGC [GA]^CCGG[CT]
2873 GCCGGC
BstAPI 2840 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3340 GCATATGCTGC
BstEII 2854 GGTGACC G^GT[ACGT]ACC
3432 GGTCACC
BstPI 2854 GGTGACC G^GT[ACGT]ACC
3432 GGTCACC
BstXI 977 CCAGAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2991 CCACTGTGATGG
CfoI 918 GCGC GCG^C
3156 GCGC
Cfr10I 1117 ACCGGC [GA]^CCGG[CT]
2873 GCCGGC
EcoO65I 2854 GGTGACC G^GT[ACGT]ACC
3432 GGTCACC
FauI 2447 CCCGC CCCGC(4/6)
751 GCGGG
HhaI 918 GCGC GCG^C
3156 GCGC
HinP1I 918 GCGC G^CGC
3156 GCGC
HindIII 981 AAGCTT A^AGCTT
2173 AAGCTT
PflMI 1203 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1567 CCACTTGATGG
PstI 2976 CTGCAG CTGCA^G
3147 CTGCAG
SmlI 2185 CTTAAG C^T[CT][GA]AG
2432 CTCAAG
Van91I 1203 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1567 CCACTTGATGG
XbaI 153 TCTAGA T^CTAGA
3186 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaII
BanI BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspLU11I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII