(Circular) (Six-base) MAPSORT of: pFN21AA0365.seq Check: 4891 from: 1 to: 7951 pFN21AA0365 7951 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3544 4822 3544 Size: 1278 6673 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2175 5761 278 Size: 53 83 186 936 639 3586 2468 Fragments arranged by size: 3586 2468 936 639 186 83 53 AccI GT'mk_AC Cuts at: 1937 5294 1937 Size: 3357 4594 Acc65I G'GTAC_C Cuts at: 4838 5272 4838 Size: 434 7517 AclI AA'CG_TT Cuts at: 1563 6078 6451 1563 Size: 4515 373 3063 Fragments arranged by size: 4515 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2257 2936 3335 3617 3920 4323 6014 Size: 461 164 679 399 282 303 403 1691 Cuts at: 6014 7398 7666 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1691 1384 679 461 403 399 303 282 268 164 AflII C'TTAA_G Cuts at: 820 1017 2131 820 Size: 197 1114 6640 Fragments arranged by size: 6640 1114 197 AflIII A'CryG_T Cuts at: 1203 3790 6850 7827 1203 Size: 2587 3060 977 1327 Fragments arranged by size: 3060 2587 1327 977 AgeI A'CCGG_T Cuts at: 6782 6782 Size: 7951 AhdI GACnn_n'nnGTC Cuts at: 6680 6680 Size: 7951 AleI CACnn'nnGTG Cuts at: 1166 4560 1166 Size: 3394 4557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7951 AlwNI CAG_nnn'CTG Cuts at: 2704 5029 5059 5694 7266 2704 Size: 2325 30 635 1572 3389 Fragments arranged by size: 3389 2325 1572 635 30 ApaLI G'TGCA_C Cuts at: 6008 7164 6008 Size: 1156 6795 ApoI r'AATT_y Cuts at: 1493 2339 2414 3041 3161 3699 4487 5260 5486 Size: 846 75 627 120 538 788 773 226 Cuts at: 5486 5638 7747 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 846 788 773 627 538 226 152 120 75 AseI AT'TA_AT Cuts at: 160 1032 6505 160 Size: 872 5473 1606 Fragments arranged by size: 5473 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7951 AvaI C'yCGr_G Cuts at: 1870 1943 2358 3996 4968 5276 1870 Size: 73 415 1638 972 308 4545 Fragments arranged by size: 4545 1638 972 415 308 73 AvrII C'CTAG_G Cuts at: 2680 2680 Size: 7951 BamHI G'GATC_C Cuts at: 5281 5648 5281 Size: 367 7584 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3767 4124 4838 5272 6727 Size: 325 217 627 1980 357 714 434 1455 Cuts at: 6727 618 Size: 1842 Fragments arranged by size: 1980 1842 1455 714 627 434 357 325 217 BanII G_rGCy'C Cuts at: 1391 2650 4050 4068 4595 4638 4667 5099 5270 Size: 1259 1400 18 527 43 29 432 171 Cuts at: 5270 1391 Size: 4072 Fragments arranged by size: 4072 1400 1259 527 432 171 43 29 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3248 3380 3564 4358 4635 5193 928 Size: 971 1349 132 184 794 277 558 3686 Fragments arranged by size: 3686 1349 971 794 558 277 184 132 BbvCI CC'TCA_GC Cuts at: 3961 3961 Size: 7951 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6142 6176 1903 Size: 34 4205 34 3678 Fragments arranged by size: 4205 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5843 7053 5843 Size: 1210 6741 BclI T'GATC_A Cuts at: 1548 1548 Size: 7951 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4779 6562 136 Size: 107 122 71 631 605 3107 1783 1525 Fragments arranged by size: 3107 1783 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2125 7946 2125 Size: 5821 2130 BlpI GC'TnA_GC Cuts at: 4653 5368 4653 Size: 715 7236 Bme1580I G_kGCm'C Cuts at: 1790 2807 3770 6012 7168 1790 Size: 1017 963 2242 1156 2573 Fragments arranged by size: 2573 2242 1156 1017 963 BmgBI CAC'GTC Cuts at: 1318 2581 4536 1318 Size: 1263 1955 4733 Fragments arranged by size: 4733 1955 1263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4220 5682 6640 Size: 416 53 83 399 24 2803 1462 958 Cuts at: 6640 442 Size: 1753 Fragments arranged by size: 2803 1753 1462 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7951 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2620 4931 2620 Size: 2311 5640 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3614 3899 4140 4679 4752 4827 6611 1239 Size: 2375 285 241 539 73 75 1784 2579 Fragments arranged by size: 2579 2375 1784 539 285 241 75 73 Bpu10I CC'TnA_GC Cuts at: 2612 2715 3961 4392 2612 Size: 103 1246 431 6171 Fragments arranged by size: 6171 1246 431 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2106 2448 3998 4044 6076 6941 7239 2106 Size: 342 1550 46 2032 865 298 2818 Fragments arranged by size: 2818 2032 1550 865 342 298 46 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6614 882 Size: 617 5115 2219 Fragments arranged by size: 5115 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5742 493 Size: 711 4538 2702 Fragments arranged by size: 4538 2702 711 BsaBI GATnn'nnATC Cuts at: 7945 7945 Size: 7951 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2172 5758 6140 6775 Size: 53 83 186 936 639 3586 382 635 Cuts at: 6775 275 Size: 1451 Fragments arranged by size: 3586 1451 936 639 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2379 6384 6782 7056 7203 7614 2379 Size: 4005 398 274 147 411 2716 Fragments arranged by size: 4005 2716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4306 4336 4525 4555 1178 Size: 30 165 30 2903 30 189 30 4574 Fragments arranged by size: 4574 2903 189 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3680 3683 3954 4040 4099 4230 4697 4700 Size: 2494 3 271 86 59 131 467 3 Cuts at: 4700 4730 4822 1186 Size: 30 92 4315 Fragments arranged by size: 4315 2494 467 271 131 92 86 59 30 3 3 BseYI C'CCAG_C Cuts at: 3741 4250 5019 7154 7650 3741 Size: 509 769 2135 496 4042 Fragments arranged by size: 4042 2135 769 509 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3993 1163 Size: 573 2257 5121 Fragments arranged by size: 5121 2257 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4147 5401 6162 7190 1540 Size: 275 186 2146 1254 761 1028 2301 Fragments arranged by size: 2301 2146 1254 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2650 2759 3135 4060 4813 5181 6012 6097 7168 Size: 109 376 925 753 368 831 85 1071 Cuts at: 7168 2650 Size: 3433 Fragments arranged by size: 3433 1071 925 831 753 376 368 109 85 BsmI GAATG_Cn' Cuts at: 5462 5555 5462 Size: 93 7858 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2650 2759 2807 3135 3770 4050 4060 Size: 399 860 109 48 328 635 280 10 Cuts at: 4060 4068 4595 4638 4667 4813 5099 5181 5270 Size: 8 527 43 29 146 286 82 89 Cuts at: 5270 6012 6097 7168 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 860 742 635 527 399 328 286 280 146 109 89 85 82 48 43 29 10 8 BspEI T'CCGG_A Cuts at: 7614 7614 Size: 7951 BspHI T'CATG_A Cuts at: 5840 7570 5840 Size: 1730 6221 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3544 4822 4850 5306 844 Size: 2700 1278 28 456 3489 Fragments arranged by size: 3489 2700 1278 456 28 BsrBI CCG'CTC Cuts at: 1394 1823 4727 5838 1394 Size: 429 2904 1111 3507 Fragments arranged by size: 3507 2904 1111 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3352 6446 6620 65 Size: 1183 2104 3094 174 1396 Fragments arranged by size: 3094 2104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3306 4146 5122 6595 6782 1700 Size: 110 1496 840 976 1473 187 2869 Fragments arranged by size: 2869 1496 1473 976 840 187 110 BsrGI T'GTAC_A Cuts at: 96 5226 96 Size: 5130 2821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7951 BssSI C'ACGA_G Cuts at: 6011 7023 6011 Size: 1012 6939 BstAPI GCAn_nnn'nTGC Cuts at: 794 3971 794 Size: 3177 4774 BstBI TT'CG_AA Cuts at: 3555 4948 6755 3555 Size: 1393 1807 4751 Fragments arranged by size: 4751 1807 1393 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7951 BstXI CCAn_nnnn'nTGG Cuts at: 2183 2372 5008 2183 Size: 189 2636 5126 Fragments arranged by size: 5126 2636 189 BstYI r'GATC_y Cuts at: 1972 2125 2147 2204 2332 2525 2895 2911 5281 Size: 153 22 57 128 193 370 16 2370 Cuts at: 5281 5648 6033 6050 7502 7588 7600 7946 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2370 1977 1452 385 370 367 346 193 153 128 86 57 22 17 16 12 Bsu36I CC'TnA_GG Cuts at: 4120 4242 4264 4680 4834 4120 Size: 122 22 416 154 7237 Fragments arranged by size: 7237 416 154 122 22 BtgI C'CryG_G Cuts at: 513 1065 2004 2425 4031 4141 4575 4596 4643 Size: 552 939 421 1606 110 434 21 47 Cuts at: 4643 5086 513 Size: 443 3378 Fragments arranged by size: 3378 1606 939 552 443 434 421 110 47 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2196 487 Size: 683 56 970 6242 Fragments arranged by size: 6242 970 683 56 BtsI GCAGTG_nn' Cuts at: 3981 5467 6261 6281 3981 Size: 1486 794 20 5651 Fragments arranged by size: 5651 1486 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7951 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2305 2340 3553 3588 529 Size: 35 1741 35 1213 35 4892 Fragments arranged by size: 4892 1741 1213 35 35 35 DraI TTT'AAA Cuts at: 2116 3168 3213 5255 2116 Size: 1052 45 2042 4812 Fragments arranged by size: 4812 2042 1052 45 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6958 809 Size: 507 5642 1802 Fragments arranged by size: 5642 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2326 2374 2449 Size: 54 1425 50 275 18 496 48 75 Cuts at: 2449 4144 5398 6287 7916 8 Size: 1695 1254 889 1629 43 Fragments arranged by size: 1695 1629 1425 1254 889 496 275 75 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4144 5398 1537 Size: 275 2332 1254 4090 Fragments arranged by size: 4090 2332 1254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2227 3311 3481 3834 4324 4850 5881 Size: 759 115 1084 170 353 490 526 1031 Cuts at: 5881 1353 Size: 3423 Fragments arranged by size: 3423 1084 1031 759 526 490 353 170 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6538 6912 7058 387 Size: 1064 138 4949 374 146 1280 Fragments arranged by size: 4949 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2648 2648 Size: 7951 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2257 2936 3335 3614 3617 3899 Size: 393 461 164 679 399 279 3 282 Cuts at: 3899 3920 4140 4323 4679 4752 4827 6014 6611 Size: 21 220 183 356 73 75 1187 597 Cuts at: 6611 7398 7666 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1187 787 679 597 461 399 393 356 282 279 268 220 183 164 75 73 21 3 EcoNI CCTnn'n_nnAGG Cuts at: 2684 4166 4960 5105 2684 Size: 1482 794 145 5530 Fragments arranged by size: 5530 1482 794 145 EcoO109I rG'GnC_Cy Cuts at: 2264 2701 3841 4101 4246 4720 2264 Size: 437 1140 260 145 474 5495 Fragments arranged by size: 5495 1140 474 437 260 145 EcoRI G'AATT_C Cuts at: 5260 5638 5260 Size: 378 7573 EcoRV GAT'ATC Cuts at: 2069 2875 2069 Size: 806 7145 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2304 2965 3326 2304 Size: 661 361 6929 Fragments arranged by size: 6929 661 361 FspI TGC'GCA Cuts at: 6457 6457 Size: 7951 HaeII r_GCGC'y Cuts at: 1678 7098 1678 Size: 5420 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 2510 2542 3612 Size: 32 135 32 609 32 297 32 1070 Cuts at: 3612 3644 4281 4306 4313 4338 4392 4424 4882 Size: 32 637 25 7 25 54 32 458 Cuts at: 4882 4897 4914 4929 6592 6624 6666 6698 1373 Size: 15 17 15 1663 32 42 32 2626 Fragments arranged by size: 2626 1663 1070 637 609 458 297 135 54 42 32 32 32 32 32 32 32 32 25 25 17 15 15 7 HincII GTy'rAC Cuts at: 669 1657 1938 2662 5295 5541 669 Size: 988 281 724 2633 246 3079 Fragments arranged by size: 3079 2633 988 724 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7951 HpaI GTT'AAC Cuts at: 5541 5541 Size: 7951 KpnI G_GTAC'C Cuts at: 4842 5276 4842 Size: 434 7517 MfeI C'AATT_G Cuts at: 5550 5550 Size: 7951 MluI A'CGCG_T Cuts at: 7827 7827 Size: 7951 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4210 5230 7065 7249 1305 Size: 2905 1020 1835 184 2007 Fragments arranged by size: 2905 2007 1835 1020 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2376 2451 10 Size: 54 1425 343 544 75 5510 Fragments arranged by size: 5510 1425 544 343 75 54 MslI CAynn'nnrTG Cuts at: 518 1166 2076 2181 3112 4346 4560 5909 6268 Size: 648 910 105 931 1234 214 1349 359 Cuts at: 6268 6427 518 Size: 159 2042 Fragments arranged by size: 2042 1349 1234 931 910 648 359 214 159 105 MspA1I CmG'CkG Cuts at: 1606 3618 3919 4039 4645 5367 6044 7192 7437 Size: 2012 301 120 606 722 677 1148 245 Cuts at: 7437 1606 Size: 2120 Fragments arranged by size: 2120 2012 1148 722 677 606 301 245 120 NaeI GCC'GGC Cuts at: 4148 4148 Size: 7951 NcoI C'CATG_G Cuts at: 513 1065 2004 5086 513 Size: 552 939 3082 3378 Fragments arranged by size: 3378 3082 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7951 NgoMIV G'CCGG_C Cuts at: 4146 4146 Size: 7951 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7951 NotI GC'GGCC_GC Cuts at: 5398 5398 Size: 7951 NspI r_CATG'y Cuts at: 1132 1229 3220 5309 6854 1132 Size: 97 1991 2089 1545 2229 Fragments arranged by size: 2229 2089 1991 1545 97 PasI CC'CwG_GG Cuts at: 1348 2848 2861 1348 Size: 1500 13 6438 Fragments arranged by size: 6438 1500 13 PciI A'CATG_T Cuts at: 6850 6850 Size: 7951 PflMI CCAn_nnn'nTGG Cuts at: 1108 5092 1108 Size: 3984 3967 PfoI T'CCnGG_A Cuts at: 4729 4920 4729 Size: 191 7760 PmeI GTTT'AAAC Cuts at: 5255 5255 Size: 7951 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6020 6052 7533 7565 6020 Size: 32 1481 32 6406 Fragments arranged by size: 6406 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3841 4101 4246 3841 Size: 260 145 7546 Fragments arranged by size: 7546 260 145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7951 PsiI TTA'TAA Cuts at: 5521 5521 Size: 7951 PstI C_TGCA'G Cuts at: 830 2509 4369 5064 5303 830 Size: 1679 1860 695 239 3478 Fragments arranged by size: 3478 1860 1679 695 239 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7951 SacI G_AGCT'C Cuts at: 2650 2650 Size: 7951 SacII CC_GC'GG Cuts at: 4646 4646 Size: 7951 SalI G'TCGA_C Cuts at: 1936 5293 1936 Size: 3357 4594 SanDI GG'GwC_CC Cuts at: 4101 4101 Size: 7951 SbfI CC_TGCA'GG Cuts at: 5303 5303 Size: 7951 ScaI AGT'ACT Cuts at: 6199 7911 6199 Size: 1712 6239 SexAI A'CCwGG_T Cuts at: 2364 2364 Size: 7951 SfcI C'TryA_G Cuts at: 826 1045 2208 2505 4365 5060 5299 6434 7115 Size: 219 1163 297 1860 695 239 1135 681 Cuts at: 7115 7306 826 Size: 191 1471 Fragments arranged by size: 1860 1471 1163 1135 695 681 297 239 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7951 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7951 SmaI CCC'GGG Cuts at: 1872 3998 5278 1872 Size: 2126 1280 4545 Fragments arranged by size: 4545 2126 1280 SmlI C'TyrA_G Cuts at: 820 1017 1943 2121 2131 2463 4013 4059 6055 Size: 197 926 178 10 332 1550 46 1996 Cuts at: 6055 6956 7218 820 Size: 901 262 1553 Fragments arranged by size: 1996 1553 1550 926 901 332 262 197 178 46 10 SnaBI TAC'GTA Cuts at: 493 5742 493 Size: 5249 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7951 SphI G_CATG'C Cuts at: 1132 5309 1132 Size: 4177 3774 SspI AAT'ATT Cuts at: 5 52 3890 5875 5 Size: 47 3838 1985 2081 Fragments arranged by size: 3838 2081 1985 47 StuI AGG'CCT Cuts at: 1517 2241 3602 4161 4829 4966 1517 Size: 724 1361 559 668 137 4502 Fragments arranged by size: 4502 1361 724 668 559 137 StyI C'CwwG_G Cuts at: 513 1065 2004 2680 3468 3708 4206 5001 5086 Size: 552 939 676 788 240 498 795 85 Cuts at: 5086 5390 513 Size: 304 3074 Fragments arranged by size: 3074 939 795 788 676 552 498 304 240 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3205 5048 5150 5978 5995 6148 6333 Size: 1255 1625 1843 102 828 17 153 185 Cuts at: 6333 6778 325 Size: 445 1498 Fragments arranged by size: 1843 1625 1498 1255 828 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3285 3582 3990 Size: 274 80 33 51 1443 1308 297 408 Cuts at: 3990 5226 6197 7909 96 Size: 1236 971 1712 138 Fragments arranged by size: 1712 1443 1308 1236 971 408 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2818 2850 4525 4557 7911 7943 2818 Size: 32 1675 32 3354 32 2826 Fragments arranged by size: 3354 2826 1675 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2366 1316 Size: 1050 6901 XbaI T'CTAG_A Cuts at: 2219 5287 2219 Size: 3068 4883 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7951 XmaI C'CCGG_G Cuts at: 1870 3996 5276 1870 Size: 2126 1280 4545 Fragments arranged by size: 4545 2126 1280 XmnI GAAnn'nnTTC Cuts at: 2106 4944 6080 2106 Size: 2838 1136 3977 Fragments arranged by size: 3977 2838 1136 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2173 5759 276 Size: 53 83 186 936 639 3586 2468 Fragments arranged by size: 3586 2468 936 639 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI SwaI XcmI