Restriction map for
pFN21AA0356
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII Acc65I AccIII AclI AflII
Aor51HI AscI AseI AsnI Asp718I
AspI AvrII BalI BcgI BciVI
BclI BfrI BlnI BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspLU11I BssSI Bst1107I BstAPI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluNI MroI MscI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PmeI PshBI
Psp1406I PvuI RsrII SalI SfiI
SnaBI SphI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 2962 GTCTAC GT^[AC][GT]AC
AgeI 908 ACCGGT A^CCGGT
AhdI 2017 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2979 GTGCAC G^TGCAC
ApaLI 2979 GTGCAC G^TGCAC
AspEI 2017 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2491 TGCGCA TGC^GCA
BamHI 70 GGATCC G^GATCC
BbrPI 1114 CACGTG CAC^GTG
BglII 3005 AGATCT A^GATCT
BlpI 2826 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2826 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 2075 TGTACA T^GTACA
BsrGI 2075 TGTACA T^GTACA
BssHII 1815 GCGCGC G^CGCGC
BstBI 526 TTCGAA TT^CGAA
BstEII 2358 GGTGACC G^GT[ACGT]ACC
BstPI 2358 GGTGACC G^GT[ACGT]ACC
CelII 2826 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2017 GACTGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1657 CTCTTC CTCTTC(1/4)
EcoO65I 2358 GGTGACC G^GT[ACGT]ACC
EcoRI 934 GAATTC G^AATTC
FspI 2491 TGCGCA TGC^GCA
HincII 331 GTCAAC GT[CT]^[GA]AC
HindII 331 GTCAAC GT[CT]^[GA]AC
Ksp632I 1657 CTCTTC CTCTTC(1/4)
KspI 1991 CCGCGG CCGC^GG
MluI 2714 ACGCGT A^CGCGT
MseI 553 TTAA T^TAA
NaeI 3134 GCCGGC GCC^GGC
NgoMIV 3134 GCCGGC G^CCGGC
NspV 526 TTCGAA TT^CGAA
PinAI 908 ACCGGT A^CCGGT
PmaCI 1114 CACGTG CAC^GTG
PmlI 1114 CACGTG CAC^GTG
PshAI 324 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1991 CCGCGG CCGC^GG
SapI 1656 GCTCTTC GCTCTTC(1/4)
SbfI 501 CCTGCAGG CCTGCA^GG
SexAI 2441 ACCTGGT A^CC[AT]GGT
SfuI 526 TTCGAA TT^CGAA
SgrAI 3133 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 121 ACTAGT A^CTAGT
Sse8387I 501 CCTGCAGG CCTGCA^GG
SspBI 2075 TGTACA T^GTACA
Tru9I 553 TTAA T^TAA
XcmI 1083 CCAGGCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 98 ACGTGT A^C[GA][CT]GT
2714 ACGCGT
AocI 1178 CCTCAGG CC^T[ACGT]AGG
1930 CCTGAGG
ApaI 2223 GGGCCC GGGCC^C
2681 GGGCCC
BfaI 122 CTAG C^TAG
1196 CTAG
BsaAI 97 TACGTG [CT]AC^GT[GA]
1114 CACGTG
BsaWI 908 ACCGGT [AT]^CCGG[AT]
1565 ACCGGA
BsmBI 2717 CGTCTC CGTCTC(1/5)
2760 GAGACG
BspHI 1260 TCATGA T^CATGA
1604 TCATGA
BsrBI 1624 GAGCGG CCGCTC(-3/-3)
2737 GAGCGG
BsrDI 847 CATTGC GCAATG(2/0)
1636 GCAATG
Bsu36I 1178 CCTCAGG CC^T[ACGT]AGG
1930 CCTGAGG
DraIII 144 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2176 CACGGCGTG
Eco81I 1178 CCTCAGG CC^T[ACGT]AGG
1930 CCTGAGG
MaeI 122 CTAG C^TAG
1196 CTAG
NspI 2730 GCATGT [GA]CATG^[CT]
2958 GCATGT
PflMI 2175 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3096 CCAGGCTGTGG
PpuMI 2086 AGGACCT [GA]G^G[AT]CC[CT]
2624 GGGACCT
PvuII 781 CAGCTG CAG^CTG
2770 CAGCTG
RcaI 1260 TCATGA T^CATGA
1604 TCATGA
ScaI 440 AGTACT AGT^ACT
1198 AGTACT
SmaI 1352 CCCGGG CCC^GGG
2414 CCCGGG
TaqI 527 TCGA T^CGA
695 TCGA
TthHB8I 527 TCGA T^CGA
695 TCGA
Van91I 2175 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3096 CCAGGCTGTGG
XmaCI 1352 CCCGGG C^CCGGG
2414 CCCGGG
XmaI 1352 CCCGGG C^CCGGG
2414 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TfiI TseI Tsp45I Tsp509I TspRI
XhoII XmnI