(Circular) (Six-base) MAPSORT of: pFN21AA0356.seq Check: 4275 from: 1 to: 7873 pFN21AA0356 7873 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3076 3076 Size: 7873 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5683 278 Size: 53 83 186 936 4147 2468 Fragments arranged by size: 4147 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4968 5216 1937 Size: 3031 248 4594 Fragments arranged by size: 4594 3031 248 Acc65I G'GTAC_C Cuts at: 5194 5194 Size: 7873 AclI AA'CG_TT Cuts at: 1563 6000 6373 1563 Size: 4437 373 3063 Fragments arranged by size: 4437 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 2667 2712 3302 3366 4289 4685 4799 Size: 797 238 45 590 64 923 396 114 Cuts at: 4799 4844 5936 7320 7588 1632 Size: 45 1092 1384 268 1917 Fragments arranged by size: 1917 1384 1092 923 797 590 396 268 238 114 64 45 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7676 AflIII A'CryG_T Cuts at: 1203 2103 4719 6772 7749 1203 Size: 900 2616 2053 977 1327 Fragments arranged by size: 2616 2053 1327 977 900 AgeI A'CCGG_T Cuts at: 2913 6704 2913 Size: 3791 4082 AhdI GACnn_n'nnGTC Cuts at: 4027 6602 4027 Size: 2575 5298 AleI CACnn'nnGTG Cuts at: 1166 2345 3720 3722 1166 Size: 1179 1375 2 5317 Fragments arranged by size: 5317 1375 1179 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3068 1184 Size: 1884 5989 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2474 2506 2474 Size: 32 7841 AlwNI CAG_nnn'CTG Cuts at: 2690 2743 3098 4095 4447 4663 5616 7188 2690 Size: 53 355 997 352 216 953 1572 3375 Fragments arranged by size: 3375 1572 997 953 355 352 216 53 ApaI G_GGCC'C Cuts at: 4232 4690 4232 Size: 458 7415 ApaLI G'TGCA_C Cuts at: 4984 5930 7086 4984 Size: 946 1156 5771 Fragments arranged by size: 5771 1156 946 ApoI r'AATT_y Cuts at: 1493 2939 4409 4949 5182 5408 5560 7669 1493 Size: 1446 1470 540 233 226 152 2109 1697 Fragments arranged by size: 2109 1697 1470 1446 540 233 226 152 AseI AT'TA_AT Cuts at: 160 1032 6427 160 Size: 872 5395 1606 Fragments arranged by size: 5395 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7873 AvaI C'yCGr_G Cuts at: 1870 1943 2713 3357 3947 4419 5198 1870 Size: 73 770 644 590 472 779 4545 Fragments arranged by size: 4545 779 770 644 590 472 73 BamHI G'GATC_C Cuts at: 2075 5203 5570 2075 Size: 3128 367 4378 Fragments arranged by size: 4378 3128 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 3142 3620 3952 4817 Size: 325 217 627 1277 78 478 332 865 Cuts at: 4817 5194 6649 618 Size: 377 1455 1842 Fragments arranged by size: 1842 1455 1277 865 627 478 377 332 325 217 78 BanII G_rGCy'C Cuts at: 1391 2865 3078 3663 3675 3996 4232 4690 4833 Size: 1474 213 585 12 321 236 458 143 Cuts at: 4833 5192 1391 Size: 359 4072 Fragments arranged by size: 4072 1474 585 458 359 321 236 213 143 12 BbeI G_GCGC'C Cuts at: 3146 3956 4821 3146 Size: 810 865 6198 Fragments arranged by size: 6198 865 810 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 2260 2700 3787 5077 928 Size: 971 240 121 440 1087 1290 3724 Fragments arranged by size: 3724 1290 1087 971 440 240 121 BbvCI CC'TCA_GC Cuts at: 2211 2478 2759 2901 3843 3891 2211 Size: 267 281 142 942 48 6193 Fragments arranged by size: 6193 942 281 267 142 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6064 6098 1903 Size: 34 50 34 4043 34 3678 Fragments arranged by size: 4043 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5765 6975 5765 Size: 1210 6663 BclI T'GATC_A Cuts at: 1548 1548 Size: 7873 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2168 4227 4358 Size: 107 122 71 631 605 496 2059 131 Cuts at: 4358 4815 6484 136 Size: 457 1669 1525 Fragments arranged by size: 2059 1669 1525 631 605 496 457 131 122 107 71 BglII A'GATC_T Cuts at: 5010 7868 5010 Size: 2858 5015 BlpI GC'TnA_GC Cuts at: 4832 5290 4832 Size: 458 7415 Bme1580I G_kGCm'C Cuts at: 1790 2242 2891 3623 4013 4232 4690 4988 5137 Size: 452 649 732 390 219 458 298 149 Cuts at: 5137 5934 7090 1790 Size: 797 1156 2573 Fragments arranged by size: 2573 1156 797 732 649 458 452 390 298 219 149 BmgBI CAC'GTC Cuts at: 1318 2520 1318 Size: 1202 6671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4636 5604 6562 Size: 416 53 83 399 24 3219 968 958 Cuts at: 6562 442 Size: 1753 Fragments arranged by size: 3219 1753 968 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7873 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2851 3199 3751 2851 Size: 348 552 6973 Fragments arranged by size: 6973 552 348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 3392 3399 3704 3989 6533 1239 Size: 947 1206 7 305 285 2544 2579 Fragments arranged by size: 2579 2544 1206 947 305 285 7 Bpu10I CC'TnA_GC Cuts at: 2211 2478 2498 2759 2901 3701 3843 3891 2211 Size: 267 20 261 142 800 142 48 6193 Fragments arranged by size: 6193 800 267 261 142 142 48 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3090 3435 4919 5998 6863 7161 3090 Size: 345 1484 1079 865 298 3802 Fragments arranged by size: 3802 1484 1079 865 345 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2922 3437 3611 4184 6536 882 Size: 617 1423 515 174 573 2352 2219 Fragments arranged by size: 2352 2219 1423 617 573 515 174 BsaAI yAC'GTr Cuts at: 493 1204 2104 3121 5664 493 Size: 711 900 1017 2543 2702 Fragments arranged by size: 2702 2543 1017 900 711 BsaBI GATnn'nnATC Cuts at: 7867 7867 Size: 7873 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3143 3953 4818 5680 Size: 53 83 186 936 1610 810 865 862 Cuts at: 5680 6062 6697 275 Size: 382 635 1451 Fragments arranged by size: 1610 1451 936 865 862 810 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2913 3570 6306 6704 6978 7125 7536 2913 Size: 657 2736 398 274 147 411 3250 Fragments arranged by size: 3250 2736 657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2474 2504 2852 2882 3890 Size: 30 165 30 1071 30 348 30 1008 Cuts at: 3890 3920 1178 Size: 30 5131 Fragments arranged by size: 5131 1071 1008 348 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 2743 2873 3725 3817 4370 4777 1186 Size: 1309 248 130 852 92 553 407 4282 Fragments arranged by size: 4282 1309 852 553 407 248 130 92 BseYI C'CCAG_C Cuts at: 2259 2784 3146 3616 4689 4959 4989 7076 7572 Size: 525 362 470 1073 270 30 2087 496 Cuts at: 7572 2259 Size: 2560 Fragments arranged by size: 2560 2087 1073 525 496 470 362 270 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2176 2658 3038 3143 3938 1163 Size: 573 438 2 482 380 105 795 5098 Fragments arranged by size: 5098 795 573 482 438 380 105 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5323 6084 7112 1540 Size: 275 186 3322 761 1028 2301 Fragments arranged by size: 3322 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 3182 3663 3675 3717 4833 4988 5934 6019 Size: 857 481 12 42 1116 155 946 85 Cuts at: 6019 7090 2325 Size: 1071 3108 Fragments arranged by size: 3108 1116 1071 946 857 481 155 85 42 12 BsmI GAATG_Cn' Cuts at: 5384 5477 5384 Size: 93 7780 BsmBI CGTCTCn'nnnn_ Cuts at: 4728 4759 4728 Size: 31 7842 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2242 2325 2865 2891 3078 3182 3623 Size: 399 452 83 540 26 187 104 441 Cuts at: 3623 3663 3675 3717 3996 4013 4232 4690 4833 Size: 40 12 42 279 17 219 458 143 Cuts at: 4833 4988 5137 5192 5934 6019 7090 1391 Size: 155 149 55 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 458 452 441 399 279 219 187 155 149 143 104 85 83 55 42 40 26 17 12 BspEI T'CCGG_A Cuts at: 7536 7536 Size: 7873 BspHI T'CATG_A Cuts at: 3265 3609 5762 7492 3265 Size: 344 2153 1730 3646 Fragments arranged by size: 3646 2153 1730 344 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3060 3076 3990 4713 5228 844 Size: 2216 16 914 723 515 3489 Fragments arranged by size: 3489 2216 914 723 515 16 BsrBI CCG'CTC Cuts at: 1394 1823 3631 4744 5760 1394 Size: 429 1808 1113 1016 3507 Fragments arranged by size: 3507 1808 1113 1016 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2851 3648 6368 6542 65 Size: 1183 1603 797 2720 174 1396 Fragments arranged by size: 2720 1603 1396 1183 797 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2913 3348 5139 6517 6704 1700 Size: 110 1103 435 1791 1378 187 2869 Fragments arranged by size: 2869 1791 1378 1103 435 187 110 BsrGI T'GTAC_A Cuts at: 96 4080 96 Size: 3984 3889 BssHII G'CGCG_C Cuts at: 1925 3820 1925 Size: 1895 5978 BssSI C'ACGA_G Cuts at: 5933 6945 5933 Size: 1012 6861 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7873 BstBI TT'CG_AA Cuts at: 2532 6677 2532 Size: 4145 3728 BstEII G'GTnAC_C Cuts at: 1184 4363 1184 Size: 3179 4694 BstXI CCAn_nnnn'nTGG Cuts at: 2831 3154 3229 3242 3776 2831 Size: 323 75 13 534 6928 Fragments arranged by size: 6928 534 323 75 13 BstYI r'GATC_y Cuts at: 1972 2075 2997 3327 4312 4680 5010 5203 5570 Size: 103 922 330 985 368 330 193 367 Cuts at: 5570 5955 5972 7424 7510 7522 7868 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 985 922 385 368 367 346 330 330 193 103 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3184 3936 3184 Size: 752 7121 BtgI C'CryG_G Cuts at: 513 1065 2182 3028 3624 3996 4180 4461 513 Size: 552 1117 846 596 372 184 281 3925 Fragments arranged by size: 3925 1117 846 596 552 372 281 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7134 Fragments arranged by size: 7134 683 56 BtsI GCAGTG_nn' Cuts at: 4972 5389 6183 6203 4972 Size: 417 794 20 6642 Fragments arranged by size: 6642 794 417 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7873 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7838 DraI TTT'AAA Cuts at: 5177 5177 Size: 7873 DraIII CAC_nnn'GTG Cuts at: 2154 4186 2154 Size: 2032 5841 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2120 2702 3418 5057 6880 809 Size: 507 804 582 716 1639 1823 1802 Fragments arranged by size: 1823 1802 1639 804 716 582 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2772 4254 4608 Size: 54 1425 50 275 18 942 1482 354 Cuts at: 4608 5320 6209 7838 8 Size: 712 889 1629 43 Fragments arranged by size: 1629 1482 1425 942 889 712 354 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5320 1537 Size: 275 3508 4090 Fragments arranged by size: 4090 3508 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3668 5803 1353 Size: 2315 2135 3423 Fragments arranged by size: 3423 2315 2135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3680 4021 6460 6834 6980 387 Size: 1064 138 2091 341 2439 374 146 1280 Fragments arranged by size: 2439 2091 1280 1064 374 341 146 138 EcoICRI GAG'CTC Cuts at: 3661 3673 4831 3661 Size: 12 1158 6703 Fragments arranged by size: 6703 1158 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2429 2667 2712 3302 3366 3392 Size: 393 554 243 238 45 590 64 26 Cuts at: 3392 3399 3704 3989 4289 4685 4799 4844 5936 Size: 7 305 285 300 396 114 45 1092 Cuts at: 5936 6533 7320 7588 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1092 787 597 590 554 396 393 305 300 285 268 243 238 114 64 45 45 26 7 EcoNI CCTnn'n_nnAGG Cuts at: 3251 3507 3878 3251 Size: 256 371 7246 Fragments arranged by size: 7246 371 256 EcoO109I rG'GnC_Cy Cuts at: 2512 3871 4092 4228 4630 4660 4939 2512 Size: 1359 221 136 402 30 279 5446 Fragments arranged by size: 5446 1359 402 279 221 136 30 EcoRI G'AATT_C Cuts at: 2939 5182 5560 2939 Size: 2243 378 5252 Fragments arranged by size: 5252 2243 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3679 5101 3679 Size: 1422 6451 FspI TGC'GCA Cuts at: 4498 6379 4498 Size: 1881 5992 HaeII r_GCGC'y Cuts at: 1678 2163 3146 3784 3956 4645 4821 7020 1678 Size: 485 983 638 172 689 176 2199 2531 Fragments arranged by size: 2531 2199 983 689 638 485 176 172 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 2852 2884 3971 Size: 32 135 32 1037 32 211 32 1087 Cuts at: 3971 4003 4013 4045 4527 4559 4580 4612 6514 Size: 32 10 32 482 32 21 32 1902 Cuts at: 6514 6546 6588 6620 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1902 1087 1037 482 211 135 42 32 32 32 32 32 32 32 32 32 32 21 10 HincII GTy'rAC Cuts at: 669 1657 1938 2338 5217 5463 669 Size: 988 281 400 2879 246 3079 Fragments arranged by size: 3079 2879 988 400 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7873 HpaI GTT'AAC Cuts at: 5463 5463 Size: 7873 KasI G'GCGC_C Cuts at: 3142 3952 4817 3142 Size: 810 865 6198 Fragments arranged by size: 6198 865 810 KpnI G_GTAC'C Cuts at: 5198 5198 Size: 7873 MfeI C'AATT_G Cuts at: 5472 5472 Size: 7873 MluI A'CGCG_T Cuts at: 4719 7749 4719 Size: 3030 4843 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 2916 3615 4924 6987 7171 1305 Size: 993 618 699 1309 2063 184 2007 Fragments arranged by size: 2063 2007 1309 993 699 618 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6051 Fragments arranged by size: 6051 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 3720 3722 3774 5831 6190 6349 Size: 648 1179 1375 2 52 2057 359 159 Cuts at: 6349 518 Size: 2042 Fragments arranged by size: 2057 2042 1375 1179 648 359 159 52 2 MspA1I CmG'CkG Cuts at: 1606 2355 2650 2788 3682 3998 4777 5130 5289 Size: 749 295 138 894 316 779 353 159 Cuts at: 5289 5966 7114 7359 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 894 779 749 677 353 316 295 245 159 138 NaeI GCC'GGC Cuts at: 5141 5141 Size: 7873 NarI GG'CG_CC Cuts at: 3143 3953 4818 3143 Size: 810 865 6198 Fragments arranged by size: 6198 865 810 NcoI C'CATG_G Cuts at: 513 1065 2182 3028 4461 513 Size: 552 1117 846 1433 3925 Fragments arranged by size: 3925 1433 1117 846 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7873 NgoMIV G'CCGG_C Cuts at: 5139 5139 Size: 7873 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7873 NotI GC'GGCC_GC Cuts at: 5320 5320 Size: 7873 NspI r_CATG'y Cuts at: 1132 1229 4739 4967 5231 6776 1132 Size: 97 3510 228 264 1545 2229 Fragments arranged by size: 3510 2229 1545 264 228 97 PasI CC'CwG_GG Cuts at: 1348 3475 3541 3958 4369 1348 Size: 2127 66 417 411 4852 Fragments arranged by size: 4852 2127 417 411 66 PciI A'CATG_T Cuts at: 6772 6772 Size: 7873 PflMI CCAn_nnn'nTGG Cuts at: 1108 4186 5107 1108 Size: 3078 921 3874 Fragments arranged by size: 3874 3078 921 PfoI T'CCnGG_A Cuts at: 2469 4197 2469 Size: 1728 6145 PmeI GTTT'AAAC Cuts at: 5177 5177 Size: 7873 PmlI CAC'GTG Cuts at: 3121 3121 Size: 7873 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2474 2506 5942 5974 7455 7487 2474 Size: 32 3436 32 1481 32 2860 Fragments arranged by size: 3436 2860 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4092 4630 4092 Size: 538 7335 PshAI GACnn'nnGTC Cuts at: 1613 2333 1613 Size: 720 7153 PsiI TTA'TAA Cuts at: 5443 5443 Size: 7873 PspOMI G'GGCC_C Cuts at: 4228 4686 4228 Size: 458 7415 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3905 3937 3905 Size: 32 7841 PstI C_TGCA'G Cuts at: 830 2424 2511 2798 3073 3252 3879 4278 4443 Size: 1594 87 287 275 179 627 399 165 Cuts at: 4443 4655 4710 5225 830 Size: 212 55 515 3478 Fragments arranged by size: 3478 1594 627 515 399 287 275 212 179 165 87 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7873 PvuII CAG'CTG Cuts at: 2788 4777 2788 Size: 1989 5884 SacI G_AGCT'C Cuts at: 3663 3675 4833 3663 Size: 12 1158 6703 Fragments arranged by size: 6703 1158 12 SacII CC_GC'GG Cuts at: 3999 3999 Size: 7873 SalI G'TCGA_C Cuts at: 1936 5215 1936 Size: 3279 4594 SapI GCTCTTCn'nnn_ Cuts at: 3668 3668 Size: 7873 SbfI CC_TGCA'GG Cuts at: 2511 5225 2511 Size: 2714 5159 ScaI AGT'ACT Cuts at: 2447 3205 6121 7833 2447 Size: 758 2916 1712 2487 Fragments arranged by size: 2916 2487 1712 758 SexAI A'CCwGG_T Cuts at: 4446 4446 Size: 7873 SfcI C'TryA_G Cuts at: 826 1045 2420 2507 2645 2794 3069 3171 3248 Size: 219 1375 87 138 149 275 102 77 Cuts at: 3248 3875 4274 4439 4651 4706 5221 6356 7037 Size: 627 399 165 212 55 515 1135 681 Cuts at: 7037 7228 826 Size: 191 1471 Fragments arranged by size: 1471 1375 1135 681 627 515 399 275 219 212 191 165 149 138 102 87 77 55 SfoI GGC'GCC Cuts at: 3144 3954 4819 3144 Size: 810 865 6198 Fragments arranged by size: 6198 865 810 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7873 SgrAI Cr'CCGG_yG Cuts at: 1700 5139 1700 Size: 3439 4434 SmaI CCC'GGG Cuts at: 1872 3359 4421 5200 1872 Size: 1487 1062 779 4545 Fragments arranged by size: 4545 1487 1062 779 SmlI C'TyrA_G Cuts at: 820 1017 1943 3105 3450 4934 5977 6878 7140 Size: 197 926 1162 345 1484 1043 901 262 Cuts at: 7140 820 Size: 1553 Fragments arranged by size: 1553 1484 1162 1043 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 5664 493 Size: 5171 2702 SpeI A'CTAG_T Cuts at: 152 2126 152 Size: 1974 5899 SphI G_CATG'C Cuts at: 1132 5231 1132 Size: 4099 3774 SspI AAT'ATT Cuts at: 5 52 5797 5 Size: 47 5745 2081 Fragments arranged by size: 5745 2081 47 StuI AGG'CCT Cuts at: 1517 2087 3014 3427 4473 1517 Size: 570 927 413 1046 4917 Fragments arranged by size: 4917 1046 927 570 413 StyI C'CwwG_G Cuts at: 513 1065 2182 3028 3235 4122 4222 4257 4461 Size: 552 1117 846 207 887 100 35 204 Cuts at: 4461 4483 5312 513 Size: 22 829 3074 Fragments arranged by size: 3074 1117 887 846 829 552 207 204 100 35 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2309 3316 3405 3898 5003 5900 5917 Size: 1255 729 1007 89 493 1105 897 17 Cuts at: 5917 6070 6255 6700 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1105 1007 897 729 493 445 185 153 89 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 3203 4074 Size: 274 80 33 51 1443 468 758 871 Cuts at: 4074 4080 6119 7831 96 Size: 6 2039 1712 138 Fragments arranged by size: 2039 1712 1443 871 758 468 274 138 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2221 2253 3890 3922 7833 7865 2221 Size: 32 1637 32 3911 32 2229 Fragments arranged by size: 3911 2229 1637 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7873 XbaI T'CTAG_A Cuts at: 5209 5209 Size: 7873 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3095 2011 Size: 1084 6789 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7873 XmaI C'CCGG_G Cuts at: 1870 3357 4419 5198 1870 Size: 1487 1062 779 4545 Fragments arranged by size: 4545 1487 1062 779 XmnI GAAnn'nnTTC Cuts at: 4139 4430 5165 6002 4139 Size: 291 735 837 6010 Fragments arranged by size: 6010 837 735 291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5681 276 Size: 53 83 186 936 4147 2468 Fragments arranged by size: 4147 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI RsrII SanDI SfiI SrfI SwaI