(Circular) (Six-base) MAPSORT of: pFN21AA0350.seq Check: 6728 from: 1 to: 7864 pFN21AA0350 7864 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2793 2859 3422 4270 4563 2793 Size: 66 563 848 293 6094 Fragments arranged by size: 6094 848 563 293 66 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5674 278 Size: 53 83 186 936 4138 2468 Fragments arranged by size: 4138 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5207 1937 Size: 3270 4594 Acc65I G'GTAC_C Cuts at: 5185 5185 Size: 7864 AclI AA'CG_TT Cuts at: 1563 3189 5991 6364 1563 Size: 1626 2802 373 3063 Fragments arranged by size: 3063 2802 1626 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3027 3731 4530 5927 7311 7579 1632 Size: 1395 704 799 1397 1384 268 1917 Fragments arranged by size: 1917 1397 1395 1384 799 704 268 AfeI AGC'GCT Cuts at: 4613 4613 Size: 7864 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7667 AflIII A'CryG_T Cuts at: 1203 2280 2880 3840 6763 7740 1203 Size: 1077 600 960 2923 977 1327 Fragments arranged by size: 2923 1327 1077 977 960 600 AgeI A'CCGG_T Cuts at: 6695 6695 Size: 7864 AhdI GACnn_n'nnGTC Cuts at: 6593 6593 Size: 7864 AleI CACnn'nnGTG Cuts at: 1166 3365 1166 Size: 2199 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3383 4478 1184 Size: 2199 1095 4570 Fragments arranged by size: 4570 2199 1095 AlwNI CAG_nnn'CTG Cuts at: 2296 4426 4555 4752 5607 7179 2296 Size: 2130 129 197 855 1572 2981 Fragments arranged by size: 2981 2130 1572 855 197 129 ApaI G_GGCC'C Cuts at: 3222 3222 Size: 7864 ApaLI G'TGCA_C Cuts at: 3755 5921 7077 3755 Size: 2166 1156 4542 Fragments arranged by size: 4542 2166 1156 ApoI r'AATT_y Cuts at: 1493 2374 2393 5173 5399 5551 7660 1493 Size: 881 19 2780 226 152 2109 1697 Fragments arranged by size: 2780 2109 1697 881 226 152 19 AseI AT'TA_AT Cuts at: 160 1032 6418 160 Size: 872 5386 1606 Fragments arranged by size: 5386 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7864 AvaI C'yCGr_G Cuts at: 1870 1943 3119 4077 5189 1870 Size: 73 1176 958 1112 4545 Fragments arranged by size: 4545 1176 1112 958 73 BamHI G'GATC_C Cuts at: 3547 5194 5561 3547 Size: 1647 367 5850 Fragments arranged by size: 5850 1647 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4581 4639 4766 5150 5185 Size: 325 217 627 2794 58 127 384 35 Cuts at: 5185 6640 618 Size: 1455 1842 Fragments arranged by size: 2794 1842 1455 627 384 325 217 127 58 35 BanII G_rGCy'C Cuts at: 1391 3222 4727 5115 5183 1391 Size: 1831 1505 388 68 4072 Fragments arranged by size: 4072 1831 1505 388 68 BbeI G_GCGC'C Cuts at: 4585 4585 Size: 7864 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2956 3246 3605 3831 3995 928 Size: 971 1057 290 359 226 164 4797 Fragments arranged by size: 4797 1057 971 359 290 226 164 BbvCI CC'TCA_GC Cuts at: 5157 5157 Size: 7864 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2723 2757 3937 3971 4118 4152 4289 Size: 34 786 34 1180 34 147 34 137 Cuts at: 4289 4323 5111 5145 6055 6089 1903 Size: 34 788 34 910 34 3678 Fragments arranged by size: 3678 1180 910 788 786 147 137 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5756 6966 5756 Size: 1210 6654 BclI T'GATC_A Cuts at: 1548 2808 3321 4092 4149 1548 Size: 1260 513 771 57 5263 Fragments arranged by size: 5263 1260 771 513 57 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3251 4731 5070 Size: 107 122 71 631 605 1579 1480 339 Cuts at: 5070 6475 136 Size: 1405 1525 Fragments arranged by size: 1579 1525 1480 1405 631 605 339 122 107 71 BglII A'GATC_T Cuts at: 7859 7859 Size: 7864 BlpI GC'TnA_GC Cuts at: 3695 5009 5139 5281 3695 Size: 1314 130 142 6278 Fragments arranged by size: 6278 1314 142 130 Bme1580I G_kGCm'C Cuts at: 1790 3222 3759 4312 5153 5925 7081 1790 Size: 1432 537 553 841 772 1156 2573 Fragments arranged by size: 2573 1432 1156 841 772 553 537 BmgBI CAC'GTC Cuts at: 1318 3754 1318 Size: 2436 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4654 5033 5595 Size: 416 53 83 399 24 3237 379 562 Cuts at: 5595 6553 442 Size: 958 1753 Fragments arranged by size: 3237 1753 958 562 416 399 379 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3623 1056 Size: 2567 5297 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2912 3710 3791 4355 4782 6524 1239 Size: 1004 669 798 81 564 427 1742 2579 Fragments arranged by size: 2579 1742 1004 798 669 564 427 81 Bpu10I CC'TnA_GC Cuts at: 4836 5157 4836 Size: 321 7543 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2066 3152 5989 6854 7152 2066 Size: 1086 2837 865 298 2778 Fragments arranged by size: 2837 2778 1086 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 2330 3166 6527 882 Size: 617 645 186 836 3361 2219 Fragments arranged by size: 3361 2219 836 645 617 186 BsaAI yAC'GTr Cuts at: 493 1204 2281 2371 2881 5655 493 Size: 711 1077 90 510 2774 2702 Fragments arranged by size: 2774 2702 1077 711 510 90 BsaBI GATnn'nnATC Cuts at: 2177 3320 7858 2177 Size: 1143 4538 2183 Fragments arranged by size: 4538 2183 1143 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3243 3906 4582 5671 Size: 53 83 186 936 1710 663 676 1089 Cuts at: 5671 6053 6688 275 Size: 382 635 1451 Fragments arranged by size: 1710 1451 1089 936 676 663 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2133 6297 6695 6969 7116 7527 2133 Size: 4164 398 274 147 411 2470 Fragments arranged by size: 4164 2470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 1178 Size: 30 165 30 648 30 6961 Fragments arranged by size: 6961 648 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2744 2925 3224 3407 3506 4097 4525 Size: 1240 318 181 299 183 99 591 428 Cuts at: 4525 1186 Size: 4525 Fragments arranged by size: 4525 1240 591 428 318 299 183 181 99 BseYI C'CCAG_C Cuts at: 2025 3083 3657 4239 4727 4895 4946 7067 7563 Size: 1058 574 582 488 168 51 2121 496 Cuts at: 7563 2025 Size: 2326 Fragments arranged by size: 2326 2121 1058 582 574 496 488 168 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 2738 2771 3389 4628 5089 1163 Size: 573 876 126 33 618 1239 461 3938 Fragments arranged by size: 3938 1239 876 618 573 461 126 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2921 3594 5314 6075 7103 1540 Size: 275 186 920 673 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 920 761 673 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 2775 2841 3428 3510 3759 4745 5925 6010 Size: 276 66 587 82 249 986 1180 85 Cuts at: 6010 7081 2499 Size: 1071 3282 Fragments arranged by size: 3282 1180 1071 986 587 276 249 85 82 66 BsmI GAATG_Cn' Cuts at: 3087 5375 5468 3087 Size: 2288 93 5483 Fragments arranged by size: 5483 2288 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2775 2841 3222 3428 3510 3759 Size: 399 709 276 66 381 206 82 249 Cuts at: 3759 4312 4727 4745 5115 5153 5183 5925 6010 Size: 553 415 18 370 38 30 742 85 Cuts at: 6010 7081 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 709 553 415 399 381 370 276 249 206 85 82 66 38 30 18 BspEI T'CCGG_A Cuts at: 7527 7527 Size: 7864 BspHI T'CATG_A Cuts at: 3642 3744 3897 5753 7483 3642 Size: 102 153 1856 1730 4023 Fragments arranged by size: 4023 1856 1730 153 102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 2793 2859 3422 4270 4563 5219 844 Size: 1304 645 66 563 848 293 656 3489 Fragments arranged by size: 3489 1304 848 656 645 563 293 66 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3124 5751 1394 Size: 429 193 1108 2627 3507 Fragments arranged by size: 3507 2627 1108 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 3437 6359 6533 65 Size: 1183 2189 2922 174 1396 Fragments arranged by size: 2922 2189 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2935 5069 5092 6508 6695 1700 Size: 110 1125 2134 23 1416 187 2869 Fragments arranged by size: 2869 2134 1416 1125 187 110 23 BsrGI T'GTAC_A Cuts at: 96 2517 4142 96 Size: 2421 1625 3818 Fragments arranged by size: 3818 2421 1625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7864 BssSI C'ACGA_G Cuts at: 2333 5924 6936 2333 Size: 3591 1012 3261 Fragments arranged by size: 3591 3261 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3093 4276 794 Size: 2299 1183 4382 Fragments arranged by size: 4382 2299 1183 BstBI TT'CG_AA Cuts at: 6668 6668 Size: 7864 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7864 BstXI CCAn_nnnn'nTGG Cuts at: 3665 3886 3665 Size: 221 7643 BstYI r'GATC_y Cuts at: 1972 2172 3547 3672 4114 5194 5561 5946 5963 Size: 200 1375 125 442 1080 367 385 17 Cuts at: 5963 7415 7501 7513 7859 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1375 1080 442 385 367 346 200 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2041 3384 4653 4923 5115 513 Size: 552 976 1343 1269 270 192 3262 Fragments arranged by size: 3262 1343 1269 976 552 270 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3993 4396 487 Size: 683 56 2767 403 3955 Fragments arranged by size: 3955 2767 683 403 56 BtsI GCAGTG_nn' Cuts at: 4034 4750 5380 6174 6194 4034 Size: 716 630 794 20 5704 Fragments arranged by size: 5704 794 716 630 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7864 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3274 3309 529 Size: 35 2710 35 5084 Fragments arranged by size: 5084 2710 35 35 DraI TTT'AAA Cuts at: 5168 5168 Size: 7864 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6871 809 Size: 507 5555 1802 Fragments arranged by size: 5555 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2918 3351 Size: 54 1425 50 275 18 181 907 433 Cuts at: 3351 3591 4428 4908 5311 6200 7829 8 Size: 240 837 480 403 889 1629 43 Fragments arranged by size: 1629 1425 907 889 837 480 433 403 275 240 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2918 3591 5311 1537 Size: 275 1106 673 1720 4090 Fragments arranged by size: 4090 1720 1106 673 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2870 3300 5118 5794 1353 Size: 1517 430 1818 676 3423 Fragments arranged by size: 3423 1818 1517 676 430 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4702 6451 6825 6971 387 Size: 1064 138 3113 1749 374 146 1280 Fragments arranged by size: 3113 1749 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2912 3027 3710 3731 3791 4355 Size: 393 611 669 115 683 21 60 564 Cuts at: 4355 4530 4782 5927 6524 7311 7579 1239 Size: 175 252 1145 597 787 268 1524 Fragments arranged by size: 1524 1145 787 683 669 611 597 564 393 268 252 175 115 60 21 EcoNI CCTnn'n_nnAGG Cuts at: 4268 4268 Size: 7864 EcoO109I rG'GnC_Cy Cuts at: 3218 4087 3218 Size: 869 6995 EcoRI G'AATT_C Cuts at: 5173 5551 5173 Size: 378 7486 FspI TGC'GCA Cuts at: 6370 6370 Size: 7864 HaeII r_GCGC'y Cuts at: 1678 3405 4426 4585 4615 7011 1678 Size: 1727 1021 159 30 2396 2531 Fragments arranged by size: 2531 2396 1727 1021 159 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6505 6537 6579 6611 1373 Size: 32 135 32 4933 32 42 32 2626 Fragments arranged by size: 4933 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4870 5044 5208 5454 669 Size: 988 281 2932 174 164 246 3079 Fragments arranged by size: 3079 2932 988 281 246 174 164 HindIII A'AGCT_T Cuts at: 748 748 Size: 7864 HpaI GTT'AAC Cuts at: 5454 5454 Size: 7864 KasI G'GCGC_C Cuts at: 4581 4581 Size: 7864 KpnI G_GTAC'C Cuts at: 5189 5189 Size: 7864 MfeI C'AATT_G Cuts at: 4040 5463 4040 Size: 1423 6441 MluI A'CGCG_T Cuts at: 7740 7740 Size: 7864 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6978 7162 1305 Size: 5673 184 2007 Fragments arranged by size: 5673 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4430 4910 10 Size: 54 1425 343 2598 480 2964 Fragments arranged by size: 2964 2598 1425 480 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2502 3365 3869 3884 4007 4280 4460 Size: 648 1336 863 504 15 123 273 180 Cuts at: 4460 5072 5822 6181 6340 518 Size: 612 750 359 159 2042 Fragments arranged by size: 2042 1336 863 750 648 612 504 359 273 180 159 123 15 MspA1I CmG'CkG Cuts at: 1606 2293 2989 4072 4404 4719 4731 4737 5083 Size: 687 696 1083 332 315 12 6 346 Cuts at: 5083 5156 5280 5957 7105 7350 1606 Size: 73 124 677 1148 245 2120 Fragments arranged by size: 2120 1148 1083 696 687 677 346 332 315 245 124 73 12 6 NaeI GCC'GGC Cuts at: 5094 5094 Size: 7864 NarI GG'CG_CC Cuts at: 4582 4582 Size: 7864 NcoI C'CATG_G Cuts at: 513 1065 2041 513 Size: 552 976 6336 Fragments arranged by size: 6336 976 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7864 NgoMIV G'CCGG_C Cuts at: 5092 5092 Size: 7864 NheI G'CTAG_C Cuts at: 1052 3619 1052 Size: 2567 5297 NotI GC'GGCC_GC Cuts at: 5311 5311 Size: 7864 NspI r_CATG'y Cuts at: 1132 1229 3844 4143 4279 5077 5136 5222 6767 Size: 97 2615 299 136 798 59 86 1545 Cuts at: 6767 1132 Size: 2229 Fragments arranged by size: 2615 2229 1545 798 299 136 97 86 59 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7864 PciI A'CATG_T Cuts at: 3840 6763 3840 Size: 2923 4941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2071 3469 1108 Size: 963 1398 5503 Fragments arranged by size: 5503 1398 963 PfoI T'CCnGG_A Cuts at: 2220 3447 4110 4482 2220 Size: 1227 663 372 5602 Fragments arranged by size: 5602 1227 663 372 PmeI GTTT'AAAC Cuts at: 5168 5168 Size: 7864 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5933 5965 7446 7478 5933 Size: 32 1481 32 6319 Fragments arranged by size: 6319 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4087 4087 Size: 7864 PshAI GACnn'nnGTC Cuts at: 1613 5030 1613 Size: 3417 4447 PsiI TTA'TAA Cuts at: 2490 3816 5434 2490 Size: 1326 1618 4920 Fragments arranged by size: 4920 1618 1326 PspOMI G'GGCC_C Cuts at: 3218 3218 Size: 7864 PspXI vC'TCGA_Gb Cuts at: 3119 3119 Size: 7864 PstI C_TGCA'G Cuts at: 830 2301 5216 830 Size: 1471 2915 3478 Fragments arranged by size: 3478 2915 1471 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7864 PvuII CAG'CTG Cuts at: 2293 2293 Size: 7864 SalI G'TCGA_C Cuts at: 1936 5206 1936 Size: 3270 4594 SapI GCTCTTCn'nnn_ Cuts at: 2870 2870 Size: 7864 SbfI CC_TGCA'GG Cuts at: 5216 5216 Size: 7864 ScaI AGT'ACT Cuts at: 6112 7824 6112 Size: 1712 6152 SfcI C'TryA_G Cuts at: 826 1045 2297 5212 6347 7028 7219 826 Size: 219 1252 2915 1135 681 191 1471 Fragments arranged by size: 2915 1471 1252 1135 681 219 191 SfoI GGC'GCC Cuts at: 4583 4583 Size: 7864 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7864 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7864 SmaI CCC'GGG Cuts at: 1872 5191 1872 Size: 3319 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 3119 3131 5968 6869 7131 Size: 197 926 138 1038 12 2837 901 262 Cuts at: 7131 820 Size: 1553 Fragments arranged by size: 2837 1553 1038 926 901 262 197 138 12 SnaBI TAC'GTA Cuts at: 493 2371 5655 493 Size: 1878 3284 2702 Fragments arranged by size: 3284 2702 1878 SpeI A'CTAG_T Cuts at: 152 152 Size: 7864 SphI G_CATG'C Cuts at: 1132 5077 5222 1132 Size: 3945 145 3774 Fragments arranged by size: 3945 3774 145 SspI AAT'ATT Cuts at: 5 52 5788 5 Size: 47 5736 2081 Fragments arranged by size: 5736 2081 47 StuI AGG'CCT Cuts at: 1517 4263 1517 Size: 2746 5118 StyI C'CwwG_G Cuts at: 513 1065 2041 2070 4155 5303 513 Size: 552 976 29 2085 1148 3074 Fragments arranged by size: 3074 2085 1148 976 552 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2017 5891 5908 6061 6246 6691 325 Size: 1255 437 3874 17 153 185 445 1498 Fragments arranged by size: 3874 1498 1255 445 437 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2517 2883 4142 Size: 274 80 33 51 1443 540 366 1259 Cuts at: 4142 6110 7822 96 Size: 1968 1712 138 Fragments arranged by size: 1968 1712 1443 1259 540 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7824 7856 7824 Size: 32 7832 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7864 XbaI T'CTAG_A Cuts at: 5200 5200 Size: 7864 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4423 4513 4757 4903 4423 Size: 90 244 146 7384 Fragments arranged by size: 7384 244 146 90 XhoI C'TCGA_G Cuts at: 1943 3119 1943 Size: 1176 6688 XmaI C'CCGG_G Cuts at: 1870 5189 1870 Size: 3319 4545 XmnI GAAnn'nnTTC Cuts at: 3012 3792 5993 3012 Size: 780 2201 4883 Fragments arranged by size: 4883 2201 780 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5672 276 Size: 53 83 186 936 4138 2468 Fragments arranged by size: 4138 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI