(Circular) (Six-base) MAPSORT of: pFN21AA0260.seq Check: 7334 from: 1 to: 5770 pFN21AA0260 5770 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2026 3580 278 Size: 53 83 186 936 490 1554 2468 Fragments arranged by size: 2468 1554 936 490 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2472 3024 3833 5217 5485 1632 Size: 515 325 552 809 1384 268 1917 Fragments arranged by size: 1917 1384 809 552 515 325 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AseI AT'TA_AT Cuts at: 160 1032 2888 4324 160 Size: 872 1856 1436 1606 Fragments arranged by size: 1856 1606 1436 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2034 3095 1870 Size: 73 91 1061 4545 Fragments arranged by size: 4545 1061 91 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3091 4546 618 Size: 325 217 627 1304 1455 1842 Fragments arranged by size: 1842 1455 1304 627 325 217 BanII G_rGCy'C Cuts at: 1391 2162 3089 1391 Size: 771 927 4072 Fragments arranged by size: 4072 927 771 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2477 928 Size: 971 578 4221 Fragments arranged by size: 4221 971 578 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 4381 136 Size: 107 122 71 631 605 583 2126 1525 Fragments arranged by size: 2126 1525 631 605 583 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 3014 3187 3014 Size: 173 5597 Bme1580I G_kGCm'C Cuts at: 1790 3831 4987 1790 Size: 2041 1156 2573 Fragments arranged by size: 2573 2041 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2480 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4430 1239 Size: 3191 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2450 3895 4760 5058 2450 Size: 1445 865 298 3162 Fragments arranged by size: 3162 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2617 2934 3561 493 Size: 711 1413 317 627 2702 Fragments arranged by size: 2702 1413 711 627 317 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2023 2099 3577 3959 Size: 53 83 186 936 490 76 1478 382 Cuts at: 3959 4594 275 Size: 635 1451 Fragments arranged by size: 1478 1451 936 635 490 382 186 83 76 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2511 2541 3054 3084 1178 Size: 30 165 30 1108 30 513 30 3864 Fragments arranged by size: 3864 1108 513 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2099 2206 1186 Size: 910 3 107 4750 Fragments arranged by size: 4750 910 107 3 BseYI C'CCAG_C Cuts at: 2089 2644 2751 2969 4973 5469 2089 Size: 555 107 218 2004 496 2390 Fragments arranged by size: 2390 2004 555 496 218 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2815 1163 Size: 573 1079 4118 Fragments arranged by size: 4118 1079 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 3220 3981 5009 1540 Size: 275 186 105 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 105 BsiHKAI G_wGCw'C Cuts at: 2162 2196 3831 3916 4987 2162 Size: 34 1635 85 1071 2945 Fragments arranged by size: 2945 1635 1071 85 34 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2196 3089 3831 3916 4987 1391 Size: 399 372 34 893 742 85 1071 2174 Fragments arranged by size: 2174 1071 893 742 399 372 85 34 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 2682 3659 5389 2682 Size: 977 1730 3063 Fragments arranged by size: 3063 1730 977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3125 844 Size: 2281 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2514 2703 2964 4265 4439 65 Size: 1183 1266 189 261 1301 174 1396 Fragments arranged by size: 1396 1301 1266 1183 261 189 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 4414 4601 1700 Size: 110 331 2273 187 2869 Fragments arranged by size: 2869 2273 331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 2609 794 Size: 1815 3955 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstYI r'GATC_y Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765 Size: 1128 367 385 17 1452 86 12 346 Cuts at: 5765 1972 Size: 1977 Fragments arranged by size: 1977 1452 1128 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 3072 3286 4080 4100 3072 Size: 214 794 20 4742 Fragments arranged by size: 4742 794 214 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 2955 3074 2955 Size: 119 5651 DraIII CAC_nnn'GTG Cuts at: 2225 2225 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2136 2236 Size: 54 1425 50 275 18 273 33 100 Cuts at: 2236 2247 2700 3217 4106 5735 8 Size: 11 453 517 889 1629 43 Fragments arranged by size: 1629 1425 889 517 453 275 273 100 54 50 43 33 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2103 3217 1537 Size: 275 291 1114 4090 Fragments arranged by size: 4090 1114 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3000 3700 1353 Size: 1647 700 3423 Fragments arranged by size: 3423 1647 700 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4357 4731 4877 387 Size: 1064 138 435 2333 374 146 1280 Fragments arranged by size: 2333 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2160 2160 Size: 5770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2472 3024 3833 4430 5217 5485 Size: 393 515 325 552 809 597 787 268 Cuts at: 5485 1239 Size: 1524 Fragments arranged by size: 1524 809 787 597 552 515 393 325 268 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 2282 4917 1678 Size: 604 2635 2531 Fragments arranged by size: 2635 2531 604 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4411 4443 4485 4517 1373 Size: 32 135 32 2839 32 42 32 2626 Fragments arranged by size: 2839 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2209 4884 5068 1305 Size: 904 2675 184 2007 Fragments arranged by size: 2675 2007 904 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2238 2249 2702 10 Size: 54 1425 343 406 11 453 3078 Fragments arranged by size: 3078 1425 453 406 343 54 11 MslI CAynn'nnrTG Cuts at: 518 1166 3728 4087 4246 518 Size: 648 2562 359 159 2042 Fragments arranged by size: 2562 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3013 3186 3863 5011 5256 1606 Size: 1407 173 677 1148 245 2120 Fragments arranged by size: 2120 1407 1148 677 245 173 NaeI GCC'GGC Cuts at: 2143 2143 Size: 5770 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NgoMIV G'CCGG_C Cuts at: 2141 2141 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2033 3128 4673 1132 Size: 97 804 1095 1545 2229 Fragments arranged by size: 2229 1545 1095 804 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 1108 Size: 1137 4633 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PmlI CAC'GTG Cuts at: 2934 2934 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 2778 3122 830 Size: 1948 344 3478 Fragments arranged by size: 3478 1948 344 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 3013 3013 Size: 5770 SacI G_AGCT'C Cuts at: 2162 2162 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SapI GCTCTTCn'nnn_ Cuts at: 3000 3000 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 2826 4018 5730 2826 Size: 1192 1712 2866 Fragments arranged by size: 2866 1712 1192 SfcI C'TryA_G Cuts at: 826 1045 2774 3118 4253 4934 5125 826 Size: 219 1729 344 1135 681 191 1471 Fragments arranged by size: 1729 1471 1135 681 344 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 3874 4775 5037 820 Size: 197 926 522 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 522 262 197 SnaBI TAC'GTA Cuts at: 493 2617 3561 493 Size: 2124 944 2702 Fragments arranged by size: 2702 2124 944 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 2033 3128 1132 Size: 901 1095 3774 Fragments arranged by size: 3774 1095 901 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 3209 513 Size: 552 939 1205 3074 Fragments arranged by size: 3074 1205 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2257 3797 3814 3967 4152 4597 325 Size: 1255 677 1540 17 153 185 445 1498 Fragments arranged by size: 1540 1498 1255 677 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2824 4016 5728 Size: 274 80 33 51 1443 847 1192 1712 Cuts at: 5728 96 Size: 138 Fragments arranged by size: 1712 1443 1192 847 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2542 2542 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2024 3578 276 Size: 53 83 186 936 490 1554 2468 Fragments arranged by size: 2468 1554 936 490 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI