(Circular) (Six-base) MAPSORT of: pFN21AA0187.seq Check: 1417 from: 1 to: 8551 pFN21AA0187 8551 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5703 5703 Size: 8551 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6361 278 Size: 53 83 186 936 4825 2468 Fragments arranged by size: 4825 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4916 5894 1937 Size: 2979 978 4594 Fragments arranged by size: 4594 2979 978 Acc65I G'GTAC_C Cuts at: 5872 5872 Size: 8551 AclI AA'CG_TT Cuts at: 1563 2495 6678 7051 1563 Size: 932 4183 373 3063 Fragments arranged by size: 4183 3063 932 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3378 3777 3795 3807 4802 5698 5764 Size: 977 769 399 18 12 995 896 66 Cuts at: 5764 5768 6614 7998 8266 1632 Size: 4 846 1384 268 1917 Fragments arranged by size: 1917 1384 995 977 896 846 769 399 268 66 18 12 4 AfeI AGC'GCT Cuts at: 2981 2981 Size: 8551 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8354 AflIII A'CryG_T Cuts at: 1203 7450 8427 1203 Size: 6247 977 1327 Fragments arranged by size: 6247 1327 977 AgeI A'CCGG_T Cuts at: 7382 7382 Size: 8551 AhdI GACnn_n'nnGTC Cuts at: 5380 5594 7280 5380 Size: 214 1686 6651 Fragments arranged by size: 6651 1686 214 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8551 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5293 5325 5293 Size: 32 8519 AlwNI CAG_nnn'CTG Cuts at: 2622 3889 6294 7866 2622 Size: 1267 2405 1572 3307 Fragments arranged by size: 3307 2405 1572 1267 ApaI G_GGCC'C Cuts at: 3108 4958 3108 Size: 1850 6701 ApaLI G'TGCA_C Cuts at: 4376 6608 7764 4376 Size: 2232 1156 5163 Fragments arranged by size: 5163 2232 1156 ApoI r'AATT_y Cuts at: 1493 2892 3931 4598 4691 5109 5168 5784 5860 Size: 1399 1039 667 93 418 59 616 76 Cuts at: 5860 6086 6238 8347 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1399 1039 667 616 418 226 152 93 76 59 AseI AT'TA_AT Cuts at: 160 1032 7105 160 Size: 872 6073 1606 Fragments arranged by size: 6073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8551 AvaI C'yCGr_G Cuts at: 1870 1943 2638 4818 5876 1870 Size: 73 695 2180 1058 4545 Fragments arranged by size: 4545 2180 1058 695 73 BamHI G'GATC_C Cuts at: 5881 6248 5881 Size: 367 8184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2642 3992 5569 5691 5872 Size: 325 217 627 855 1350 1577 122 181 Cuts at: 5872 7327 618 Size: 1455 1842 Fragments arranged by size: 1842 1577 1455 1350 855 627 325 217 181 122 BanII G_rGCy'C Cuts at: 1391 2244 3108 3539 4341 4958 5236 5870 1391 Size: 853 864 431 802 617 278 634 4072 Fragments arranged by size: 4072 864 853 802 634 617 431 278 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2201 2600 3486 3825 4017 4362 4890 Size: 971 302 399 886 339 192 345 528 Cuts at: 4890 4903 5284 928 Size: 13 381 4195 Fragments arranged by size: 4195 971 886 528 399 381 345 339 302 192 13 BbvCI CC'TCA_GC Cuts at: 4032 5835 4032 Size: 1803 6748 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2093 2127 6742 6776 1903 Size: 34 156 34 4615 34 3678 Fragments arranged by size: 4615 3678 156 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5319 6443 7653 5319 Size: 1124 1210 6217 Fragments arranged by size: 6217 1210 1124 BclI T'GATC_A Cuts at: 1548 3427 1548 Size: 1879 6672 BfrBI ATG'CAT Cuts at: 4933 4933 Size: 8551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3164 7162 Size: 107 122 71 631 605 337 1155 3998 Cuts at: 7162 136 Size: 1525 Fragments arranged by size: 3998 1525 1155 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2446 8546 2446 Size: 6100 2451 BlpI GC'TnA_GC Cuts at: 3538 5968 3538 Size: 2430 6121 Bme1580I G_kGCm'C Cuts at: 1790 3108 3997 4380 4958 6612 7768 1790 Size: 1318 889 383 578 1654 1156 2573 Fragments arranged by size: 2573 1654 1318 1156 889 578 383 BmgBI CAC'GTC Cuts at: 1318 4375 1318 Size: 3057 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3899 4246 5333 Size: 416 53 83 399 24 2482 347 1087 Cuts at: 5333 6282 7240 442 Size: 949 958 1753 Fragments arranged by size: 2482 1753 1087 958 949 416 399 347 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8551 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3553 3553 Size: 8551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 3045 3342 3654 3713 4192 5220 7211 Size: 836 970 297 312 59 479 1028 1991 Cuts at: 7211 1239 Size: 2579 Fragments arranged by size: 2579 1991 1028 970 836 479 312 297 59 Bpu10I CC'TnA_GC Cuts at: 2013 4032 5835 2013 Size: 2019 1803 4729 Fragments arranged by size: 4729 2019 1803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3488 3548 3852 3983 4796 6676 7541 7839 3488 Size: 60 304 131 813 1880 865 298 4200 Fragments arranged by size: 4200 1880 865 813 304 298 131 60 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3908 5581 7214 882 Size: 617 2409 1673 1633 2219 Fragments arranged by size: 2409 2219 1673 1633 617 BsaAI yAC'GTr Cuts at: 493 1204 4768 6342 493 Size: 711 3564 1574 2702 Fragments arranged by size: 3564 2702 1574 711 BsaBI GATnn'nnATC Cuts at: 8545 8545 Size: 8551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5424 6358 6740 7375 Size: 53 83 186 936 3891 934 382 635 Cuts at: 7375 275 Size: 1451 Fragments arranged by size: 3891 1451 936 934 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2835 6984 7382 7656 7803 8214 2835 Size: 4149 398 274 147 411 3172 Fragments arranged by size: 4149 3172 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2579 1178 Size: 30 165 30 1146 30 7150 Fragments arranged by size: 7150 1146 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2880 3419 3547 3787 4262 4349 4864 5747 Size: 1694 539 128 240 475 87 515 883 Cuts at: 5747 5750 5753 1186 Size: 3 3 3984 Fragments arranged by size: 3984 1694 883 539 515 475 240 128 87 3 3 BseYI C'CCAG_C Cuts at: 5323 7754 8250 5323 Size: 2431 496 5624 Fragments arranged by size: 5624 2431 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3577 3828 4718 4790 5480 5681 1163 Size: 573 1841 251 890 72 690 201 4033 Fragments arranged by size: 4033 1841 890 690 573 251 201 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5595 6001 6762 7790 1540 Size: 275 186 3594 406 761 1028 2301 Fragments arranged by size: 3594 2301 1028 761 406 275 186 BsiHKAI G_wGCw'C Cuts at: 2294 2872 3539 4341 4380 5236 5712 6612 6697 Size: 578 667 802 39 856 476 900 85 Cuts at: 6697 7768 2294 Size: 1071 3077 Fragments arranged by size: 3077 1071 900 856 802 667 578 476 85 39 BsmI GAATG_Cn' Cuts at: 3288 5394 6062 6155 3288 Size: 2106 668 93 5684 Fragments arranged by size: 5684 2106 668 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2094 2094 Size: 8551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2294 2872 3108 3539 3997 4341 Size: 399 454 50 578 236 431 458 344 Cuts at: 4341 4380 4958 5236 5712 5870 6612 6697 7768 Size: 39 578 278 476 158 742 85 1071 Cuts at: 7768 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 578 578 476 458 454 431 399 344 278 236 158 85 50 39 BspEI T'CCGG_A Cuts at: 8214 8214 Size: 8551 BspHI T'CATG_A Cuts at: 5611 6440 8170 5611 Size: 829 1730 5992 Fragments arranged by size: 5992 1730 829 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5703 5839 5906 844 Size: 4859 136 67 3489 Fragments arranged by size: 4859 3489 136 67 BsrBI CCG'CTC Cuts at: 1394 1823 6438 1394 Size: 429 4615 3507 Fragments arranged by size: 4615 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3745 4933 7046 7220 65 Size: 1183 2497 1188 2113 174 1396 Fragments arranged by size: 2497 2113 1396 1188 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5590 7195 7382 1700 Size: 110 3780 1605 187 2869 Fragments arranged by size: 3780 2869 1605 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8551 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8551 BssSI C'ACGA_G Cuts at: 6611 7623 6611 Size: 1012 7539 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8551 BstBI TT'CG_AA Cuts at: 5188 7355 5188 Size: 2167 6384 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8551 BstXI CCAn_nnnn'nTGG Cuts at: 4729 4729 Size: 8551 BstYI r'GATC_y Cuts at: 1972 2086 2446 4822 5622 5881 6248 6633 6650 Size: 114 360 2376 800 259 367 385 17 Cuts at: 6650 8102 8188 8200 8546 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2376 1977 1452 800 385 367 360 346 259 114 86 17 12 BstZ17I GTA'TAC Cuts at: 4917 4917 Size: 8551 Bsu36I CC'TnA_GG Cuts at: 5468 5765 5468 Size: 297 8254 BtgI C'CryG_G Cuts at: 513 1065 2004 5397 5419 513 Size: 552 939 3393 22 3645 Fragments arranged by size: 3645 3393 939 552 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4775 487 Size: 683 56 3549 4263 Fragments arranged by size: 4263 3549 683 56 BtsI GCAGTG_nn' Cuts at: 3583 3594 3883 5492 5630 6067 6861 6881 3583 Size: 11 289 1609 138 437 794 20 5253 Fragments arranged by size: 5253 1609 794 437 289 138 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8516 DraI TTT'AAA Cuts at: 2988 5855 2988 Size: 2867 5684 DraIII CAC_nnn'GTG Cuts at: 3806 3806 Size: 8551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2365 7558 809 Size: 507 1049 5193 1802 Fragments arranged by size: 5193 1802 1049 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5163 5592 5998 Size: 54 1425 50 275 18 3333 429 406 Cuts at: 5998 6887 8516 8 Size: 889 1629 43 Fragments arranged by size: 3333 1629 1425 889 429 406 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5592 5998 1537 Size: 275 3780 406 4090 Fragments arranged by size: 4090 3780 406 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2964 3396 3579 3753 3828 4128 4484 Size: 761 850 432 183 174 75 300 356 Cuts at: 4484 5783 6481 1353 Size: 1299 698 3423 Fragments arranged by size: 3423 1299 850 761 698 432 356 300 183 174 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5599 7138 7512 7658 387 Size: 1064 138 4010 1539 374 146 1280 Fragments arranged by size: 4010 1539 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3537 4339 5234 3537 Size: 802 895 6854 Fragments arranged by size: 6854 895 802 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2609 3045 3342 3378 3654 3713 Size: 393 443 534 436 297 36 276 59 Cuts at: 3713 3777 3795 3807 4192 4802 5220 5698 5764 Size: 64 18 12 385 610 418 478 66 Cuts at: 5764 5768 6614 7211 7998 8266 1239 Size: 4 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 610 597 534 478 443 436 418 393 385 297 276 268 66 64 59 36 18 12 4 EcoNI CCTnn'n_nnAGG Cuts at: 2176 4042 2176 Size: 1866 6685 EcoO109I rG'GnC_Cy Cuts at: 2270 2351 3104 4225 4394 4954 4955 5521 5761 Size: 81 753 1121 169 560 1 566 240 Cuts at: 5761 2270 Size: 5060 Fragments arranged by size: 5060 1121 753 566 560 240 169 81 1 EcoRI G'AATT_C Cuts at: 5784 5860 6238 5784 Size: 76 378 8097 Fragments arranged by size: 8097 378 76 EcoRV GAT'ATC Cuts at: 2812 2812 Size: 8551 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5794 5794 Size: 8551 FspI TGC'GCA Cuts at: 7057 7057 Size: 8551 HaeII r_GCGC'y Cuts at: 1678 2983 7698 1678 Size: 1305 4715 2531 Fragments arranged by size: 4715 2531 1305 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2257 2289 2413 2445 3338 Size: 32 135 32 685 32 124 32 893 Cuts at: 3338 3370 3412 3444 3865 3897 4812 4844 5366 Size: 32 42 32 421 32 915 32 522 Cuts at: 5366 5398 5580 5612 7192 7224 7266 7298 1373 Size: 32 182 32 1580 32 42 32 2626 Fragments arranged by size: 2626 1580 915 893 685 522 421 182 135 124 42 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2337 3067 4159 5895 6141 669 Size: 988 281 399 730 1092 1736 246 3079 Fragments arranged by size: 3079 1736 1092 988 730 399 281 246 HindIII A'AGCT_T Cuts at: 748 2133 3851 4076 748 Size: 1385 1718 225 5223 Fragments arranged by size: 5223 1718 1385 225 HpaI GTT'AAC Cuts at: 6141 6141 Size: 8551 KpnI G_GTAC'C Cuts at: 5876 5876 Size: 8551 MfeI C'AATT_G Cuts at: 5843 6150 5843 Size: 307 8244 MluI A'CGCG_T Cuts at: 8427 8427 Size: 8551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2263 3269 3351 3453 7665 7849 1305 Size: 958 1006 82 102 4212 184 2007 Fragments arranged by size: 4212 2007 1006 958 184 102 82 MscI TGG'CCA Cuts at: 10 64 1489 1832 5165 10 Size: 54 1425 343 3333 3396 Fragments arranged by size: 3396 3333 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 6509 6868 7027 518 Size: 648 1251 4092 359 159 2042 Fragments arranged by size: 4092 2042 1251 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3542 3755 4031 4585 5689 5967 6644 7792 Size: 1936 213 276 554 1104 278 677 1148 Cuts at: 7792 8037 1606 Size: 245 2120 Fragments arranged by size: 2120 1936 1148 1104 677 554 278 276 245 213 NcoI C'CATG_G Cuts at: 513 1065 2004 5419 513 Size: 552 939 3415 3645 Fragments arranged by size: 3645 3415 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8551 NotI GC'GGCC_GC Cuts at: 5998 5998 Size: 8551 NsiI A_TGCA'T Cuts at: 4935 4935 Size: 8551 NspI r_CATG'y Cuts at: 1132 1229 2902 4933 5909 7454 1132 Size: 97 1673 2031 976 1545 2229 Fragments arranged by size: 2229 2031 1673 1545 976 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8551 PciI A'CATG_T Cuts at: 7450 7450 Size: 8551 PflMI CCAn_nnn'nTGG Cuts at: 1108 2282 2707 3118 1108 Size: 1174 425 411 6541 Fragments arranged by size: 6541 1174 425 411 PmeI GTTT'AAAC Cuts at: 5855 5855 Size: 8551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5432 5464 6620 6652 8133 8165 5432 Size: 32 1156 32 1481 32 5818 Fragments arranged by size: 5818 1481 1156 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2270 5761 2270 Size: 3491 5060 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8551 PsiI TTA'TAA Cuts at: 6121 6121 Size: 8551 PspOMI G'GGCC_C Cuts at: 3104 4954 3104 Size: 1850 6701 PstI C_TGCA'G Cuts at: 830 2085 3601 5903 830 Size: 1255 1516 2302 3478 Fragments arranged by size: 3478 2302 1516 1255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8551 PvuII CAG'CTG Cuts at: 3755 4031 4585 3755 Size: 276 554 7721 Fragments arranged by size: 7721 554 276 SacI G_AGCT'C Cuts at: 3539 4341 5236 3539 Size: 802 895 6854 Fragments arranged by size: 6854 895 802 SalI G'TCGA_C Cuts at: 1936 5893 1936 Size: 3957 4594 SapI GCTCTTCn'nnn_ Cuts at: 2964 4128 5783 2964 Size: 1164 1655 5732 Fragments arranged by size: 5732 1655 1164 SbfI CC_TGCA'GG Cuts at: 5903 5903 Size: 8551 ScaI AGT'ACT Cuts at: 6799 8511 6799 Size: 1712 6839 SexAI A'CCwGG_T Cuts at: 5378 5378 Size: 8551 SfcI C'TryA_G Cuts at: 826 1045 2081 3597 4839 5034 5899 7034 7715 Size: 219 1036 1516 1242 195 865 1135 681 Cuts at: 7715 7906 826 Size: 191 1471 Fragments arranged by size: 1516 1471 1242 1135 1036 865 681 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8551 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8551 SmaI CCC'GGG Cuts at: 1872 2640 5878 1872 Size: 768 3238 4545 Fragments arranged by size: 4545 3238 768 SmlI C'TyrA_G Cuts at: 820 1017 1943 3467 3527 3867 3998 4811 6655 Size: 197 926 1524 60 340 131 813 1844 Cuts at: 6655 7556 7818 820 Size: 901 262 1553 Fragments arranged by size: 1844 1553 1524 926 901 813 340 262 197 131 60 SnaBI TAC'GTA Cuts at: 493 6342 493 Size: 5849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8551 SphI G_CATG'C Cuts at: 1132 5909 1132 Size: 4777 3774 SspI AAT'ATT Cuts at: 5 52 6475 5 Size: 47 6423 2081 Fragments arranged by size: 6423 2081 47 StuI AGG'CCT Cuts at: 1517 2740 4037 1517 Size: 1223 1297 6031 Fragments arranged by size: 6031 1297 1223 StyI C'CwwG_G Cuts at: 513 1065 2004 3070 4314 5154 5419 5679 5990 Size: 552 939 1066 1244 840 265 260 311 Cuts at: 5990 513 Size: 3074 Fragments arranged by size: 3074 1244 1066 939 840 552 311 265 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 2394 2570 3125 4721 5354 6578 Size: 1255 774 40 176 555 1596 633 1224 Cuts at: 6578 6595 6748 6933 7378 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1596 1498 1255 1224 774 633 555 445 185 176 153 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2521 6797 8509 Size: 274 80 33 51 1443 544 4276 1712 Cuts at: 8509 96 Size: 138 Fragments arranged by size: 4276 1712 1443 544 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 8511 8543 2549 Size: 32 5930 32 2557 Fragments arranged by size: 5930 2557 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3056 1316 Size: 1740 6811 XbaI T'CTAG_A Cuts at: 4129 5887 4129 Size: 1758 6793 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3115 4216 3115 Size: 1101 7450 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8551 XmaI C'CCGG_G Cuts at: 1870 2638 5876 1870 Size: 768 3238 4545 Fragments arranged by size: 4545 3238 768 XmnI GAAnn'nnTTC Cuts at: 4680 5777 6680 4680 Size: 1097 903 6551 Fragments arranged by size: 6551 1097 903 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6359 276 Size: 53 83 186 936 4825 2468 Fragments arranged by size: 4825 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BsiWI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI