Restriction map for
pFN21AA0182
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp718I AspI AviII BamHI
BciVI BclI BfrI BglII BsaBI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstYI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoRI EcoT22I FbaI FspI HpaI
KpnI MamI MfeI MflI MluI
MroI MseI MunI NdeI NotI
NruI NsiI PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SgrAI SnaBI SpeI SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XhoII
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# ONE CUT ENZYMES ( 51 enzymes) #
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AhdI 1309 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 394 GTGCAC G^TGCAC
ApaLI 394 GTGCAC G^TGCAC
Asp700I 2370 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1309 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3403 CCTAGG C^CTAGG
BalI 1035 TGGCCA TGG^CCA
BbeI 1119 GGCGCC GGCGC^C
BbrPI 400 CACGTG CAC^GTG
BbsI 1663 GTCTTC GAAGAC(2/6)
BcgI 668 CGATGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3403 CCTAGG C^CTAGG
BpuAI 1663 GTCTTC GAAGAC(2/6)
BsaAI 400 CACGTG [CT]AC^GT[GA]
BsaI 3327 GGTCTC GGTCTC(1/5)
BsaWI 1149 TCCGGT [AT]^CCGG[AT]
BsrDI 3391 GCAATG GCAATG(2/0)
BstBI 2359 TTCGAA TT^CGAA
BstEII 150 GGTGACC G^GT[ACGT]ACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
Eam1105I 1309 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1857 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
EcoRV 3422 GATATC GAT^ATC
FseI 1369 GGCCGGCC GGCCGG^CC
HincII 2950 GTTGAC GT[CT]^[GA]AC
HindII 2950 GTTGAC GT[CT]^[GA]AC
KasI 1119 GGCGCC G^GCGCC
KspI 2618 CCGCGG CCGC^GG
MluNI 1035 TGGCCA TGG^CCA
MscI 1035 TGGCCA TGG^CCA
MslI 2778 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1119 GGCGCC GG^CGCC
NheI 2676 GCTAGC G^CTAGC
NspV 2359 TTCGAA TT^CGAA
PaeR7I 1813 CTCGAG C^TCGAG
PmaCI 400 CACGTG CAC^GTG
PmlI 400 CACGTG CAC^GTG
PvuII 1072 CAGCTG CAG^CTG
SacII 2618 CCGCGG CCGC^GG
SbfI 1113 CCTGCAGG CCTGCA^GG
SfiI 978 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1119 GGCGCC GGC^GCC
SfuI 2359 TTCGAA TT^CGAA
SmlI 1813 CTCGAG C^T[CT][GA]AG
SphI 2741 GCATGC GCATG^C
Sse8387I 1113 CCTGCAGG CCTGCA^GG
TfiI 358 GATTC G^A[AT]TC
XcmI 2796 CCACAGCACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1813 CTCGAG C^TCGAG
XmnI 2370 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 2316 GTCTAC GT^[AC][GT]AC
3233 GTAGAC
AcyI 1119 GGCGCC G[GA]^CG[CT]C
2292 GACGCC
AflIII 2141 ACACGT A^C[GA][CT]GT
2954 ACACGT
AlwI 1242 GATCC GGATC(4/5)
3155 GGATC
AocI 268 CCTCAGG CC^T[ACGT]AGG
606 CCTGAGG
Aor51HI 729 AGCGCT AGC^GCT
2407 AGCGCT
BbvCI 756 CCTCAGC CCTCAGC(-5/-2)
3261 CCTCAGC
BglI 979 GCCGCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1911 GCCCTACCGGC
BsaHI 1119 GGCGCC G[GA]^CG[CT]C
2292 GACGCC
BsmBI 2526 CGTCTC CGTCTC(1/5)
1567 GAGACG
Bsu36I 268 CCTCAGG CC^T[ACGT]AGG
606 CCTGAGG
DpnII 1242 GATC ^GATC
3156 GATC
DrdI 1427 GACCAAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2953 GACACGTCCGTC
Eco47III 729 AGCGCT AGC^GCT
2407 AGCGCT
Eco81I 268 CCTCAGG CC^T[ACGT]AGG
606 CCTGAGG
HgaI 1569 GACGC GACGC(5/10)
2292 GACGC
Hin1I 1119 GGCGCC G[GA]^CG[CT]C
2292 GACGCC
HindIII 441 AAGCTT A^AGCTT
3173 AAGCTT
MboI 1242 GATC ^GATC
3156 GATC
NcoI 92 CCATGG C^CATGG
1194 CCATGG
NdeII 1242 GATC ^GATC
3156 GATC
PflMI 3063 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3288 CCACAGCGTGG
PpuMI 7 GGGTCCT [GA]G^G[AT]CC[CT]
610 AGGTCCC
SapI 2811 GAAGAGC GCTCTTC(1/4)
3193 GAAGAGC
Sau3AI 1242 GATC ^GATC
3156 GATC
Tsp509I 2207 AATT ^AATT
2274 AATT
Van91I 3063 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3288 CCACAGCGTGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV NspI PleI PstI RsaI
SacI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI