(Circular) (Six-base) MAPSORT of: pFN21AA0182.seq Check: 4484 from: 1 to: 8137 pFN21AA0182 8137 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2319 2688 3337 5209 2319 Size: 369 649 1872 5247 Fragments arranged by size: 5247 1872 649 369 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5947 278 Size: 53 83 186 936 4411 2468 Fragments arranged by size: 4411 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4322 5239 5480 1937 Size: 2385 917 241 4594 Fragments arranged by size: 4594 2385 917 241 Acc65I G'GTAC_C Cuts at: 5458 5458 Size: 8137 AclI AA'CG_TT Cuts at: 1563 6264 6637 1563 Size: 4701 373 3063 Fragments arranged by size: 4701 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2563 3125 3923 5441 6200 7584 7852 1632 Size: 931 562 798 1518 759 1384 268 1917 Fragments arranged by size: 1917 1518 1384 931 798 759 562 268 AfeI AGC'GCT Cuts at: 2736 4414 2736 Size: 1678 6459 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7940 AflIII A'CryG_T Cuts at: 1203 4146 4959 7036 8013 1203 Size: 2943 813 2077 977 1327 Fragments arranged by size: 2943 2077 1327 977 813 AgeI A'CCGG_T Cuts at: 6968 6968 Size: 8137 AhdI GACnn_n'nnGTC Cuts at: 3319 6866 3319 Size: 3547 4590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8137 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3533 3565 3533 Size: 32 8105 AlwNI CAG_nnn'CTG Cuts at: 3352 3364 4663 5880 7452 3352 Size: 12 1299 1217 1572 4037 Fragments arranged by size: 4037 1572 1299 1217 12 ApaI G_GGCC'C Cuts at: 2105 2965 3798 3804 3943 4105 5060 2105 Size: 860 833 6 139 162 955 5182 Fragments arranged by size: 5182 955 860 833 162 139 6 ApaLI G'TGCA_C Cuts at: 2399 6194 7350 2399 Size: 3795 1156 3186 Fragments arranged by size: 3795 3186 1156 ApoI r'AATT_y Cuts at: 1493 5446 5672 5824 7933 1493 Size: 3953 226 152 2109 1697 Fragments arranged by size: 3953 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6691 160 Size: 872 5659 1606 Fragments arranged by size: 5659 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8137 AvaI C'yCGr_G Cuts at: 1870 1943 2708 2964 3090 3791 3797 3818 5462 Size: 73 765 256 126 701 6 21 1644 Cuts at: 5462 1870 Size: 4545 Fragments arranged by size: 4545 1644 765 701 256 126 73 21 6 AvrII C'CTAG_G Cuts at: 5408 5408 Size: 8137 BamHI G'GATC_C Cuts at: 5467 5834 5467 Size: 367 7770 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2410 3124 3157 3178 3385 Size: 325 217 627 623 714 33 21 207 Cuts at: 3385 5458 6913 618 Size: 2073 1455 1842 Fragments arranged by size: 2073 1842 1455 714 627 623 325 217 207 33 21 BanII G_rGCy'C Cuts at: 1391 2105 2265 2715 2965 3003 3627 3798 3804 Size: 714 160 450 250 38 624 171 6 Cuts at: 3804 3819 3891 3943 4105 4836 5040 5060 5456 Size: 15 72 52 162 731 204 20 396 Cuts at: 5456 1391 Size: 4072 Fragments arranged by size: 4072 731 714 624 450 396 250 204 171 162 160 72 52 38 20 15 6 BbeI G_GCGC'C Cuts at: 3128 3128 Size: 8137 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3661 928 Size: 971 1762 5404 Fragments arranged by size: 5404 1762 971 BbvCI CC'TCA_GC Cuts at: 2762 5267 2762 Size: 2505 5632 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2662 2696 6328 6362 1903 Size: 34 725 34 3632 34 3678 Fragments arranged by size: 3678 3632 725 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6029 7239 6029 Size: 1210 6927 BclI T'GATC_A Cuts at: 1548 1548 Size: 8137 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2990 3922 6748 Size: 107 122 71 631 605 1318 932 2826 Cuts at: 6748 136 Size: 1525 Fragments arranged by size: 2826 1525 1318 932 631 605 122 107 71 BglII A'GATC_T Cuts at: 8132 8132 Size: 8137 BlpI GC'TnA_GC Cuts at: 2315 3813 3890 4712 5554 2315 Size: 1498 77 822 842 4898 Fragments arranged by size: 4898 1498 842 822 77 Bme1580I G_kGCm'C Cuts at: 1790 2097 2105 2139 2403 2415 2965 3162 3183 Size: 307 8 34 264 12 550 197 21 Cuts at: 3183 3798 3804 3943 4105 5060 6198 7354 1790 Size: 615 6 139 162 955 1138 1156 2573 Fragments arranged by size: 2573 1156 1138 955 615 550 307 264 197 162 139 34 21 12 8 6 BmgBI CAC'GTC Cuts at: 1318 4149 4962 1318 Size: 2831 813 4493 Fragments arranged by size: 4493 2831 813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2412 3923 5868 Size: 416 53 83 399 24 995 1511 1945 Cuts at: 5868 6826 442 Size: 958 1753 Fragments arranged by size: 1945 1753 1511 995 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4685 1056 Size: 3629 4508 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5305 5305 Size: 8137 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2206 2212 2372 2960 3017 3374 3791 4031 Size: 967 6 160 588 57 357 417 240 Cuts at: 4031 4746 4981 5231 5267 5336 6797 1239 Size: 715 235 250 36 69 1461 2579 Fragments arranged by size: 2579 1461 967 715 588 417 357 250 240 235 160 69 57 36 6 Bpu10I CC'TnA_GC Cuts at: 2762 2795 4983 5267 2762 Size: 33 2188 284 5632 Fragments arranged by size: 5632 2188 284 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6262 7127 7425 6262 Size: 865 298 6974 Fragments arranged by size: 6974 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5338 6800 882 Size: 617 3839 1462 2219 Fragments arranged by size: 3839 2219 1462 617 BsaAI yAC'GTr Cuts at: 493 1204 2407 5928 493 Size: 711 1203 3521 2702 Fragments arranged by size: 3521 2702 1203 711 BsaBI GATnn'nnATC Cuts at: 8131 8131 Size: 8137 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3125 4298 5944 6326 Size: 53 83 186 936 1592 1173 1646 382 Cuts at: 6326 6961 275 Size: 635 1451 Fragments arranged by size: 1646 1592 1451 1173 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3154 6570 6968 7242 7389 7800 3154 Size: 3416 398 274 147 411 3491 Fragments arranged by size: 3491 3416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 2149 2179 2218 Size: 30 165 30 648 30 68 30 39 Cuts at: 2218 2248 2370 2400 2738 2768 3257 3287 4031 Size: 30 122 30 338 30 489 30 744 Cuts at: 4031 4061 4495 4525 1178 Size: 30 434 30 4790 Fragments arranged by size: 4790 744 648 489 434 338 165 122 68 39 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2209 2804 3068 3278 3281 3704 4028 4103 Size: 1023 595 264 210 3 423 324 75 Cuts at: 4103 4106 5105 5108 5117 5144 5326 1186 Size: 3 999 3 9 27 182 3997 Fragments arranged by size: 3997 1023 999 595 423 324 264 210 182 75 27 9 3 3 3 BseYI C'CCAG_C Cuts at: 2138 2345 2540 3002 3182 3510 3626 5016 7340 Size: 207 195 462 180 328 116 1390 2324 Cuts at: 7340 7836 2138 Size: 496 2439 Fragments arranged by size: 2439 2324 1390 496 462 328 207 195 180 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 2645 2666 2723 3507 3584 4755 Size: 573 642 267 21 57 784 77 1171 Cuts at: 4755 1163 Size: 4545 Fragments arranged by size: 4545 1171 784 642 573 267 77 57 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5587 6348 7376 1540 Size: 275 186 3586 761 1028 2301 Fragments arranged by size: 3586 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2403 2715 3405 3419 3777 3819 3891 6198 6283 Size: 312 690 14 358 42 72 2307 85 Cuts at: 6283 7354 2403 Size: 1071 3186 Fragments arranged by size: 3186 2307 1071 690 358 312 85 72 42 14 BsmI GAATG_Cn' Cuts at: 5648 5741 5648 Size: 93 8044 BsmBI CGTCTCn'nnnn_ Cuts at: 3566 4537 3566 Size: 971 7166 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2105 2139 2265 2403 2415 2715 Size: 399 307 8 34 126 138 12 300 Cuts at: 2715 2965 3003 3162 3183 3405 3419 3627 3777 Size: 250 38 159 21 222 14 208 150 Cuts at: 3777 3798 3804 3819 3891 3943 4105 4836 5040 Size: 21 6 15 72 52 162 731 204 Cuts at: 5040 5060 5456 6198 6283 7354 1391 Size: 20 396 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 731 399 396 307 300 250 222 208 204 162 159 150 138 126 85 72 52 38 34 21 21 20 15 14 12 8 6 BspEI T'CCGG_A Cuts at: 7800 7800 Size: 8137 BspHI T'CATG_A Cuts at: 6026 7756 6026 Size: 1730 6407 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2319 2688 3337 5209 5492 844 Size: 1475 369 649 1872 283 3489 Fragments arranged by size: 3489 1872 1475 649 369 283 BsrBI CCG'CTC Cuts at: 1394 1823 2269 2854 3058 3664 5215 5347 6024 Size: 429 446 585 204 606 1551 132 677 Cuts at: 6024 1394 Size: 3507 Fragments arranged by size: 3507 1551 677 606 585 446 429 204 132 BsrDI GCAATG_nn' Cuts at: 65 1248 5403 6632 6806 65 Size: 1183 4155 1229 174 1396 Fragments arranged by size: 4155 1396 1229 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 2216 3315 3375 3388 3921 4582 Size: 110 395 11 1099 60 13 533 661 Cuts at: 4582 6781 6968 1700 Size: 2199 187 2869 Fragments arranged by size: 2869 2199 1099 661 533 395 187 110 60 13 11 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8137 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8137 BssSI C'ACGA_G Cuts at: 3443 3753 4868 6197 7209 3443 Size: 310 1115 1329 1012 4371 Fragments arranged by size: 4371 1329 1115 1012 310 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 3157 3349 794 Size: 2240 123 192 5582 Fragments arranged by size: 5582 2240 192 123 BstBI TT'CG_AA Cuts at: 4365 6941 4365 Size: 2576 5561 BstEII G'GTnAC_C Cuts at: 1184 2155 1184 Size: 971 7166 BstXI CCAn_nnnn'nTGG Cuts at: 2548 2995 5024 2548 Size: 447 2029 5661 Fragments arranged by size: 5661 2029 447 BstYI r'GATC_y Cuts at: 1972 5467 5834 6219 6236 7688 7774 7786 8132 Size: 3495 367 385 17 1452 86 12 346 Cuts at: 8132 1972 Size: 1977 Fragments arranged by size: 3495 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2274 2612 2274 Size: 338 7799 BtgI C'CryG_G Cuts at: 513 1065 2097 2151 3015 3199 3528 3698 3935 Size: 552 1032 54 864 184 329 170 237 Cuts at: 3935 4623 4826 513 Size: 688 203 3824 Fragments arranged by size: 3824 1032 864 688 552 329 237 203 184 170 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2146 2687 4098 487 Size: 683 56 920 541 1411 4526 Fragments arranged by size: 4526 1411 920 683 541 56 BtsI GCAGTG_nn' Cuts at: 2239 2489 2517 2657 3232 4494 5043 5653 6447 Size: 250 28 140 575 1262 549 610 794 Cuts at: 6447 6467 2239 Size: 20 3909 Fragments arranged by size: 3909 1262 794 610 575 549 250 140 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8137 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4094 4129 529 Size: 35 3530 35 4537 Fragments arranged by size: 4537 3530 35 35 DraI TTT'AAA Cuts at: 5441 5441 Size: 8137 DraIII CAC_nnn'GTG Cuts at: 2134 2407 2410 3178 5299 2134 Size: 273 3 768 2121 4972 Fragments arranged by size: 4972 2121 768 273 3 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3438 4964 7144 809 Size: 507 2122 1526 2180 1802 Fragments arranged by size: 2180 2122 1802 1526 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2514 2982 Size: 54 1425 50 275 18 222 462 468 Cuts at: 2982 3018 3040 3454 3873 4119 4491 5584 6473 Size: 36 22 414 419 246 372 1093 889 Cuts at: 6473 8102 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1093 889 468 462 419 414 372 275 246 222 54 50 43 36 22 18 EagI C'GGCC_G Cuts at: 1537 1812 5584 1537 Size: 275 3772 4090 Fragments arranged by size: 4090 3772 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4415 4811 4842 5094 5193 6067 1353 Size: 3062 396 31 252 99 874 3423 Fragments arranged by size: 3423 3062 874 396 252 99 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3407 3732 4255 5318 6724 7098 Size: 1064 138 1818 325 523 1063 1406 374 Cuts at: 7098 7244 387 Size: 146 1280 Fragments arranged by size: 1818 1406 1280 1064 1063 523 374 325 146 138 EcoICRI GAG'CTC Cuts at: 2713 3817 3889 2713 Size: 1104 72 6961 Fragments arranged by size: 6961 1104 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2212 2372 2563 2960 3017 3125 Size: 393 574 6 160 191 397 57 108 Cuts at: 3125 3374 3791 3923 4031 4746 4981 5231 5267 Size: 249 417 132 108 715 235 250 36 Cuts at: 5267 5336 5441 6200 6797 7584 7852 1239 Size: 69 105 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 715 597 574 417 397 393 268 250 249 235 191 160 132 108 108 105 69 57 36 6 EcoNI CCTnn'n_nnAGG Cuts at: 3866 3866 Size: 8137 EcoO109I rG'GnC_Cy Cuts at: 2013 2102 2616 2992 3801 3940 4101 4720 4897 Size: 89 514 376 809 139 161 619 177 Cuts at: 4897 5056 5057 2013 Size: 159 1 5093 Fragments arranged by size: 5093 809 619 514 376 177 161 159 139 89 1 EcoRI G'AATT_C Cuts at: 5446 5824 5446 Size: 378 7759 EcoRV GAT'ATC Cuts at: 5429 5429 Size: 8137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4457 4457 Size: 8137 FseI GG_CCGG'CC Cuts at: 3379 3379 Size: 8137 FspI TGC'GCA Cuts at: 6643 6643 Size: 8137 HaeII r_GCGC'y Cuts at: 1678 2738 2947 3128 3985 4319 4416 4770 7284 Size: 1060 209 181 857 334 97 354 2514 Cuts at: 7284 1678 Size: 2531 Fragments arranged by size: 2531 2514 1060 857 354 334 209 181 97 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 2204 2236 2283 Size: 32 135 32 577 32 23 32 47 Cuts at: 2283 2315 2738 2770 3305 3337 4136 4168 4495 Size: 32 423 32 535 32 799 32 327 Cuts at: 4495 4507 4527 4539 4976 5008 5069 5101 6778 Size: 12 20 12 437 32 61 32 1677 Cuts at: 6778 6810 6852 6884 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1677 799 577 535 437 423 327 135 61 47 42 32 32 32 32 32 32 32 32 32 32 32 32 23 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4957 5481 5727 669 Size: 988 281 3019 524 246 3079 Fragments arranged by size: 3079 3019 988 524 281 246 HindIII A'AGCT_T Cuts at: 748 2446 5178 748 Size: 1698 2732 3707 Fragments arranged by size: 3707 2732 1698 HpaI GTT'AAC Cuts at: 5727 5727 Size: 8137 KasI G'GCGC_C Cuts at: 3124 3124 Size: 8137 KpnI G_GTAC'C Cuts at: 5462 5462 Size: 8137 MfeI C'AATT_G Cuts at: 5736 5736 Size: 8137 MluI A'CGCG_T Cuts at: 8013 8013 Size: 8137 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2192 3163 3219 5055 7251 7435 1305 Size: 887 971 56 1836 2196 184 2007 Fragments arranged by size: 2196 2007 1836 971 887 184 56 MscI TGG'CCA Cuts at: 10 64 1489 1832 3042 10 Size: 54 1425 343 1210 5105 Fragments arranged by size: 5105 1425 1210 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4787 6095 6454 6613 518 Size: 648 3621 1308 359 159 2042 Fragments arranged by size: 3621 2042 1308 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2392 2950 2959 3079 3289 3649 3853 3894 Size: 786 558 9 120 210 360 204 41 Cuts at: 3894 4625 4695 4795 5553 6230 7378 7623 1606 Size: 731 70 100 758 677 1148 245 2120 Fragments arranged by size: 2120 1148 786 758 731 677 558 360 245 210 204 120 100 70 41 9 NaeI GCC'GGC Cuts at: 2207 2218 3377 3390 2207 Size: 11 1159 13 6954 Fragments arranged by size: 6954 1159 13 11 NarI GG'CG_CC Cuts at: 3125 3125 Size: 8137 NcoI C'CATG_G Cuts at: 513 1065 2097 3199 513 Size: 552 1032 1102 5451 Fragments arranged by size: 5451 1102 1032 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8137 NgoMIV G'CCGG_C Cuts at: 2205 2216 3375 3388 2205 Size: 11 1159 13 6954 Fragments arranged by size: 6954 1159 13 11 NheI G'CTAG_C Cuts at: 1052 4681 1052 Size: 3629 4508 NotI GC'GGCC_GC Cuts at: 5584 5584 Size: 8137 NspI r_CATG'y Cuts at: 1132 1229 2485 2755 4750 5495 7040 1132 Size: 97 1256 270 1995 745 1545 2229 Fragments arranged by size: 2229 1995 1545 1256 745 270 97 PasI CC'CwG_GG Cuts at: 1348 2120 5433 1348 Size: 772 3313 4052 Fragments arranged by size: 4052 3313 772 PciI A'CATG_T Cuts at: 7036 7036 Size: 8137 PflMI CCAn_nnn'nTGG Cuts at: 1108 5074 5299 1108 Size: 3966 225 3946 Fragments arranged by size: 3966 3946 225 PfoI T'CCnGG_A Cuts at: 2222 4674 2222 Size: 2452 5685 PmeI GTTT'AAAC Cuts at: 5441 5441 Size: 8137 PmlI CAC'GTG Cuts at: 2407 2407 Size: 8137 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6206 6238 7719 7751 6206 Size: 32 1481 32 6592 Fragments arranged by size: 6592 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2013 2616 2013 Size: 603 7534 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8137 PsiI TTA'TAA Cuts at: 5707 5707 Size: 8137 PspOMI G'GGCC_C Cuts at: 2101 2961 3794 3800 3939 4101 5056 2101 Size: 860 833 6 139 162 955 5182 Fragments arranged by size: 5182 955 860 833 162 139 6 PstI C_TGCA'G Cuts at: 830 2527 2781 3123 3978 4884 4905 4939 4992 Size: 1697 254 342 855 906 21 34 53 Cuts at: 4992 5489 830 Size: 497 3478 Fragments arranged by size: 3478 1697 906 855 497 342 254 53 34 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8137 PvuII CAG'CTG Cuts at: 3079 3079 Size: 8137 SacI G_AGCT'C Cuts at: 2715 3819 3891 2715 Size: 1104 72 6961 Fragments arranged by size: 6961 1104 72 SacII CC_GC'GG Cuts at: 4626 4626 Size: 8137 SalI G'TCGA_C Cuts at: 1936 5479 1936 Size: 3543 4594 SapI GCTCTTCn'nnn_ Cuts at: 4811 5193 4811 Size: 382 7755 SbfI CC_TGCA'GG Cuts at: 3123 5489 3123 Size: 2366 5771 ScaI AGT'ACT Cuts at: 6385 8097 6385 Size: 1712 6425 SfcI C'TryA_G Cuts at: 826 1045 2523 2777 3119 3974 4330 4880 4901 Size: 219 1478 254 342 855 356 550 21 Cuts at: 4901 4935 4988 5260 5485 6620 7301 7492 826 Size: 34 53 272 225 1135 681 191 1471 Fragments arranged by size: 1478 1471 1135 855 681 550 356 342 272 254 225 219 191 53 34 21 SfiI GGCCn_nnn'nGGCC Cuts at: 2990 2990 Size: 8137 SfoI GGC'GCC Cuts at: 3126 3126 Size: 8137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8137 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8137 SmaI CCC'GGG Cuts at: 1872 2966 3793 3799 5464 1872 Size: 1094 827 6 1665 4545 Fragments arranged by size: 4545 1665 1094 827 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 3818 6241 7142 7404 820 Size: 197 926 1875 2423 901 262 1553 Fragments arranged by size: 2423 1875 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5928 493 Size: 5435 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8137 SphI G_CATG'C Cuts at: 1132 4750 5495 1132 Size: 3618 745 3774 Fragments arranged by size: 3774 3618 745 SrfI GCCC'GGGC Cuts at: 3793 3799 3793 Size: 6 8131 SspI AAT'ATT Cuts at: 5 52 6061 5 Size: 47 6009 2081 Fragments arranged by size: 6009 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8137 StyI C'CwwG_G Cuts at: 513 1065 2097 2988 3199 3567 4776 5408 5576 Size: 552 1032 891 211 368 1209 632 168 Cuts at: 5576 513 Size: 3074 Fragments arranged by size: 3074 1209 1032 891 632 552 368 211 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2098 2444 2679 3541 3845 4208 4789 Size: 1255 518 346 235 862 304 363 581 Cuts at: 4789 4904 6164 6181 6334 6519 6964 325 Size: 115 1260 17 153 185 445 1498 Fragments arranged by size: 1498 1260 1255 862 581 518 445 363 346 304 235 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6383 8095 96 Size: 274 80 33 51 1443 4406 1712 138 Fragments arranged by size: 4406 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2218 2250 2370 2402 2547 2579 3257 3289 4848 Size: 32 120 32 145 32 678 32 1559 Cuts at: 4848 4880 8097 8129 2218 Size: 32 3217 32 2226 Fragments arranged by size: 3217 2226 1559 678 145 120 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8137 XbaI T'CTAG_A Cuts at: 5473 5473 Size: 8137 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4808 4808 Size: 8137 XhoI C'TCGA_G Cuts at: 1943 3818 1943 Size: 1875 6262 XmaI C'CCGG_G Cuts at: 1870 2964 3791 3797 5462 1870 Size: 1094 827 6 1665 4545 Fragments arranged by size: 4545 1665 1094 827 6 XmnI GAAnn'nnTTC Cuts at: 4379 6266 4379 Size: 1887 6250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5945 276 Size: 53 83 186 936 4411 2468 Fragments arranged by size: 4411 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SwaI