Restriction map for
pFN21AA0182
(Linear) (Six-base) MAPSORT of: KIAA0182.seq Check: 8966 from: 1 to: 3432
KIAA0182.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 314 683 1332 3204 3432
Size: 314 369 649 1872 228
Fragments arranged by size:
1872 649 369 314 228
AccI GT'mk_AC
Cuts at: 0 2317 3234 3432
Size: 2317 917 198
Fragments arranged by size:
2317 917 198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 1120 1918 3432
Size: 558 562 798 1514
Fragments arranged by size:
1514 798 562 558
AfeI AGC'GCT
Cuts at: 0 731 2409 3432
Size: 731 1678 1023
Fragments arranged by size:
1678 1023 731
AflIII A'CryG_T
Cuts at: 0 2141 2954 3432
Size: 2141 813 478
Fragments arranged by size:
2141 813 478
AhdI GACnn_n'nnGTC
Cuts at: 0 1314 3432
Size: 1314 2118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1528 1560 3432
Size: 1528 32 1872
Fragments arranged by size:
1872 1528 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1347 1359 2658 3432
Size: 1347 12 1299 774
Fragments arranged by size:
1347 1299 774 12
ApaI G_GGCC'C
Cuts at: 0 100 960 1793 1799 1938 2100 3055 3432
Size: 100 860 833 6 139 162 955 377
Fragments arranged by size:
955 860 833 377 162 139 100 6
ApaLI G'TGCA_C
Cuts at: 0 394 3432
Size: 394 3038
AvaI C'yCGr_G
Cuts at: 0 703 959 1085 1786 1792 1813 3432
Size: 703 256 126 701 6 21 1619
Fragments arranged by size:
1619 703 701 256 126 21 6
AvrII C'CTAG_G
Cuts at: 0 3403 3432
Size: 3403 29
BanI G'GyrC_C
Cuts at: 0 405 1119 1152 1173 1380 3432
Size: 405 714 33 21 207 2052
Fragments arranged by size:
2052 714 405 207 33 21
BanII G_rGCy'C
Cuts at: 0 100 260 710 960 998 1622 1793 1799
Size: 100 160 450 250 38 624 171 6
Cuts at: 1799 1814 1886 1938 2100 2831 3035 3055 3432
Size: 15 72 52 162 731 204 20 377
Fragments arranged by size:
731 624 450 377 250 204 171 162
160 100 72 52 38 20 15 6
BbeI G_GCGC'C
Cuts at: 0 1123 3432
Size: 1123 2309
BbsI GAAGACnn'nnnn_
Cuts at: 0 1656 3432
Size: 1656 1776
BbvCI CC'TCA_GC
Cuts at: 0 757 3262 3432
Size: 757 2505 170
Fragments arranged by size:
2505 757 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 657 691 3432
Size: 657 34 2741
Fragments arranged by size:
2741 657 34
BglI GCCn_nnn'nGGC
Cuts at: 0 985 1917 3432
Size: 985 932 1515
Fragments arranged by size:
1515 985 932
BlpI GC'TnA_GC
Cuts at: 0 310 1808 1885 2707 3432
Size: 310 1498 77 822 725
Fragments arranged by size:
1498 822 725 310 77
Bme1580I G_kGCm'C
Cuts at: 0 92 100 134 398 410 960 1157 1178
Size: 92 8 34 264 12 550 197 21
Cuts at: 1178 1793 1799 1938 2100 3055 3432
Size: 615 6 139 162 955 377
Fragments arranged by size:
955 615 550 377 264 197 162 139
92 34 21 12 8 6
BmgBI CAC'GTC
Cuts at: 0 2144 2957 3432
Size: 2144 813 475
Fragments arranged by size:
2144 813 475
BmrI ACTGGGnnnn_n'
Cuts at: 0 407 1918 3432
Size: 407 1511 1514
Fragments arranged by size:
1514 1511 407
BmtI G_CTAG'C
Cuts at: 0 2680 3432
Size: 2680 752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3300 3432
Size: 3300 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 207 367 955 1012 1369 1786 2026
Size: 201 6 160 588 57 357 417 240
Cuts at: 2026 2741 2976 3226 3262 3331 3432
Size: 715 235 250 36 69 101
Fragments arranged by size:
715 588 417 357 250 240 235 201
160 101 69 57 36 6
Bpu10I CC'TnA_GC
Cuts at: 0 757 790 2978 3262 3432
Size: 757 33 2188 284 170
Fragments arranged by size:
2188 757 284 170 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 3333 3432
Size: 3333 99
BsaAI yAC'GTr
Cuts at: 0 402 3432
Size: 402 3030
BsaHI Gr'CG_yC
Cuts at: 0 1120 2293 3432
Size: 1120 1173 1139
Fragments arranged by size:
1173 1139 1120
BsaWI w'CCGG_w
Cuts at: 0 1149 3432
Size: 1149 2283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 144 174 213 243 365 395
Size: 46 30 68 30 39 30 122 30
Cuts at: 395 733 763 1252 1282 2026 2056 2490 2520
Size: 338 30 489 30 744 30 434 30
Cuts at: 2520 3432
Size: 912
Fragments arranged by size:
912 744 489 434 338 122 68 46
39 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 204 799 1063 1273 1276 1699 2023 2098
Size: 204 595 264 210 3 423 324 75
Cuts at: 2098 2101 3100 3103 3112 3139 3321 3432
Size: 3 999 3 9 27 182 111
Fragments arranged by size:
999 595 423 324 264 210 204 182
111 75 27 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 133 340 535 997 1177 1505 1621 3011
Size: 133 207 195 462 180 328 116 1390
Cuts at: 3011 3432
Size: 421
Fragments arranged by size:
1390 462 421 328 207 195 180 133
116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 640 661 718 1502 1579 2750 3432
Size: 373 267 21 57 784 77 1171 682
Fragments arranged by size:
1171 784 682 373 267 77 57 21
BsiHKAI G_wGCw'C
Cuts at: 0 398 710 1400 1414 1772 1814 1886 3432
Size: 398 312 690 14 358 42 72 1546
Fragments arranged by size:
1546 690 398 358 312 72 42 14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1561 2532 3432
Size: 1561 971 900
Fragments arranged by size:
1561 971 900
Bsp1286I G_dGCh'C
Cuts at: 0 92 100 134 260 398 410 710 960
Size: 92 8 34 126 138 12 300 250
Cuts at: 960 998 1157 1178 1400 1414 1622 1772 1793
Size: 38 159 21 222 14 208 150 21
Cuts at: 1793 1799 1814 1886 1938 2100 2831 3035 3055
Size: 6 15 72 52 162 731 204 20
Cuts at: 3055 3432
Size: 377
Fragments arranged by size:
731 377 300 250 222 208 204 162
159 150 138 126 92 72 52 38
34 21 21 20 15 14 12 8
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 683 1332 3204 3432
Size: 314 369 649 1872 228
Fragments arranged by size:
1872 649 369 314 228
BsrBI CCG'CTC
Cuts at: 0 264 849 1053 1659 3210 3342 3432
Size: 264 585 204 606 1551 132 90
Fragments arranged by size:
1551 606 585 264 204 132 90
BsrDI GCAATG_nn'
Cuts at: 0 3398 3432
Size: 3398 34
BsrFI r'CCGG_y
Cuts at: 0 200 211 1310 1370 1383 1916 2577 3432
Size: 200 11 1099 60 13 533 661 855
Fragments arranged by size:
1099 855 661 533 200 60 13 11
BssSI C'ACGA_G
Cuts at: 0 1438 1748 2863 3432
Size: 1438 310 1115 569
Fragments arranged by size:
1438 1115 569 310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 1152 1344 3432
Size: 1029 123 192 2088
Fragments arranged by size:
2088 1029 192 123
BstBI TT'CG_AA
Cuts at: 0 2360 3432
Size: 2360 1072
BstEII G'GTnAC_C
Cuts at: 0 150 3432
Size: 150 3282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 543 990 3019 3432
Size: 543 447 2029 413
Fragments arranged by size:
2029 543 447 413
Bsu36I CC'TnA_GG
Cuts at: 0 269 607 3432
Size: 269 338 2825
Fragments arranged by size:
2825 338 269
BtgI C'CryG_G
Cuts at: 0 92 146 1010 1194 1523 1693 1930 2618
Size: 92 54 864 184 329 170 237 688
Cuts at: 2618 2821 3432
Size: 203 611
Fragments arranged by size:
864 688 611 329 237 203 184 170
92 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 141 682 2093 3432
Size: 141 541 1411 1339
Fragments arranged by size:
1411 1339 541 141
BtsI GCAGTG_nn'
Cuts at: 0 234 484 512 652 1227 2489 3038 3432
Size: 234 250 28 140 575 1262 549 394
Fragments arranged by size:
1262 575 549 394 250 234 140 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2089 2124 3432
Size: 2089 35 1308
Fragments arranged by size:
2089 1308 35
DraIII CAC_nnn'GTG
Cuts at: 0 129 402 405 1173 3294 3432
Size: 129 273 3 768 2121 138
Fragments arranged by size:
2121 768 273 138 129 3
DrdI GACnn_nn'nnGTC
Cuts at: 0 1433 2959 3432
Size: 1433 1526 473
Fragments arranged by size:
1526 1433 473
EaeI y'GGCC_r
Cuts at: 0 47 509 977 1013 1035 1449 1868 2114
Size: 47 462 468 36 22 414 419 246
Cuts at: 2114 2486 3432
Size: 372 946
Fragments arranged by size:
946 468 462 419 414 372 246 47
36 22
EarI CTCTTCn'nnn_
Cuts at: 0 2410 2806 2837 3089 3188 3432
Size: 2410 396 31 252 99 244
Fragments arranged by size:
2410 396 252 244 99 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1402 1727 2250 3313 3432
Size: 1402 325 523 1063 119
Fragments arranged by size:
1402 1063 523 325 119
EcoICRI GAG'CTC
Cuts at: 0 708 1812 1884 3432
Size: 708 1104 72 1548
Fragments arranged by size:
1548 1104 708 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 207 367 558 955 1012 1120 1369
Size: 201 6 160 191 397 57 108 249
Cuts at: 1369 1786 1918 2026 2741 2976 3226 3262 3331
Size: 417 132 108 715 235 250 36 69
Cuts at: 3331 3432
Size: 101
Fragments arranged by size:
715 417 397 250 249 235 201 191
160 132 108 108 101 69 57 36
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1861 3432
Size: 1861 1571
EcoO109I rG'GnC_Cy
Cuts at: 0 8 97 611 987 1796 1935 2096 2715
Size: 8 89 514 376 809 139 161 619
Cuts at: 2715 2892 3051 3052 3432
Size: 177 159 1 380
Fragments arranged by size:
809 619 514 380 376 177 161 159
139 89 8 1
EcoRV GAT'ATC
Cuts at: 0 3424 3432
Size: 3424 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2452 3432
Size: 2452 980
FseI GG_CCGG'CC
Cuts at: 0 1374 3432
Size: 1374 2058
HaeII r_GCGC'y
Cuts at: 0 733 942 1123 1980 2314 2411 2765 3432
Size: 733 209 181 857 334 97 354 667
Fragments arranged by size:
857 733 667 354 334 209 181 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 199 231 278 310 733 765
Size: 144 32 23 32 47 32 423 32
Cuts at: 765 1300 1332 2131 2163 2490 2502 2522 2534
Size: 535 32 799 32 327 12 20 12
Cuts at: 2534 2971 3003 3064 3096 3432
Size: 437 32 61 32 336
Fragments arranged by size:
799 535 437 423 336 327 144 61
47 32 32 32 32 32 32 32
32 23 20 12 12
HincII GTy'rAC
Cuts at: 0 2952 3432
Size: 2952 480
HindIII A'AGCT_T
Cuts at: 0 441 3173 3432
Size: 441 2732 259
Fragments arranged by size:
2732 441 259
KasI G'GCGC_C
Cuts at: 0 1119 3432
Size: 1119 2313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1158 1214 3050 3432
Size: 187 971 56 1836 382
Fragments arranged by size:
1836 971 382 187 56
MscI TGG'CCA
Cuts at: 0 1037 3432
Size: 1037 2395
MslI CAynn'nnrTG
Cuts at: 0 2782 3432
Size: 2782 650
MspA1I CmG'CkG
Cuts at: 0 387 945 954 1074 1284 1644 1848 1889
Size: 387 558 9 120 210 360 204 41
Cuts at: 1889 2620 2690 2790 3432
Size: 731 70 100 642
Fragments arranged by size:
731 642 558 387 360 210 204 120
100 70 41 9
NaeI GCC'GGC
Cuts at: 0 202 213 1372 1385 3432
Size: 202 11 1159 13 2047
Fragments arranged by size:
2047 1159 202 13 11
NarI GG'CG_CC
Cuts at: 0 1120 3432
Size: 1120 2312
NcoI C'CATG_G
Cuts at: 0 92 1194 3432
Size: 92 1102 2238
Fragments arranged by size:
2238 1102 92
NgoMIV G'CCGG_C
Cuts at: 0 200 211 1370 1383 3432
Size: 200 11 1159 13 2049
Fragments arranged by size:
2049 1159 200 13 11
NheI G'CTAG_C
Cuts at: 0 2676 3432
Size: 2676 756
NspI r_CATG'y
Cuts at: 0 480 750 2745 3432
Size: 480 270 1995 687
Fragments arranged by size:
1995 687 480 270
PasI CC'CwG_GG
Cuts at: 0 115 3432
Size: 115 3317
PflMI CCAn_nnn'nTGG
Cuts at: 0 3069 3294 3432
Size: 3069 225 138
Fragments arranged by size:
3069 225 138
PfoI T'CCnGG_A
Cuts at: 0 217 2669 3432
Size: 217 2452 763
Fragments arranged by size:
2452 763 217
PmlI CAC'GTG
Cuts at: 0 402 3432
Size: 402 3030
PpuMI rG'GwC_Cy
Cuts at: 0 8 611 3432
Size: 8 603 2821
Fragments arranged by size:
2821 603 8
PspOMI G'GGCC_C
Cuts at: 0 96 956 1789 1795 1934 2096 3051 3432
Size: 96 860 833 6 139 162 955 381
Fragments arranged by size:
955 860 833 381 162 139 96 6
PstI C_TGCA'G
Cuts at: 0 522 776 1118 1973 2879 2900 2934 2987
Size: 522 254 342 855 906 21 34 53
Cuts at: 2987 3432
Size: 445
Fragments arranged by size:
906 855 522 445 342 254 53 34
21
PvuII CAG'CTG
Cuts at: 0 1074 3432
Size: 1074 2358
SacI G_AGCT'C
Cuts at: 0 710 1814 1886 3432
Size: 710 1104 72 1546
Fragments arranged by size:
1546 1104 710 72
SacII CC_GC'GG
Cuts at: 0 2621 3432
Size: 2621 811
SapI GCTCTTCn'nnn_
Cuts at: 0 2806 3188 3432
Size: 2806 382 244
Fragments arranged by size:
2806 382 244
SbfI CC_TGCA'GG
Cuts at: 0 1118 3432
Size: 1118 2314
SfcI C'TryA_G
Cuts at: 0 518 772 1114 1969 2325 2875 2896 2930
Size: 518 254 342 855 356 550 21 34
Cuts at: 2930 2983 3255 3432
Size: 53 272 177
Fragments arranged by size:
855 550 518 356 342 272 254 177
53 34 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 985 3432
Size: 985 2447
SfoI GGC'GCC
Cuts at: 0 1121 3432
Size: 1121 2311
SmaI CCC'GGG
Cuts at: 0 961 1788 1794 3432
Size: 961 827 6 1638
Fragments arranged by size:
1638 961 827 6
SmlI C'TyrA_G
Cuts at: 0 1813 3432
Size: 1813 1619
SphI G_CATG'C
Cuts at: 0 2745 3432
Size: 2745 687
SrfI GCCC'GGGC
Cuts at: 0 1788 1794 3432
Size: 1788 6 1638
Fragments arranged by size:
1788 1638 6
StyI C'CwwG_G
Cuts at: 0 92 983 1194 1562 2771 3403 3432
Size: 92 891 211 368 1209 632 29
Fragments arranged by size:
1209 891 632 368 211 92 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 439 674 1536 1840 2203 2784 2899
Size: 93 346 235 862 304 363 581 115
Cuts at: 2899 3432
Size: 533
Fragments arranged by size:
862 581 533 363 346 304 235 115
93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 213 245 365 397 542 574 1252 1284
Size: 213 32 120 32 145 32 678 32
Cuts at: 1284 2843 2875 3432
Size: 1559 32 557
Fragments arranged by size:
1559 678 557 213 145 120 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2803 3432
Size: 2803 629
XhoI C'TCGA_G
Cuts at: 0 1813 3432
Size: 1813 1619
XmaI C'CCGG_G
Cuts at: 0 959 1786 1792 3432
Size: 959 827 6 1640
Fragments arranged by size:
1640 959 827 6
XmnI GAAnn'nnTTC
Cuts at: 0 2374 3432
Size: 2374 1058
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AloI AlwNI
ApaI ApaLI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SapI SbfI SfcI SfiI SfoI SmaI SmlI
SphI SrfI StyI TaqII TstI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AleI AlfI ApoI
AscI AseI AsiSI BaeI BamHI BciVI BclI BfrBI
BglII BpuEI BsaBI BsiEI BsiWI BsmI BspEI BspHI
BsrGI BssHII BstYI BstZ17I ClaI DraI EagI EcoRI
FspI FspAI HpaI KpnI MfeI MluI NdeI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI
Tth111I XbaI ZraI