(Circular) (Six-base) MAPSORT of: pFN21AA0179.seq Check: 8593 from: 1 to: 6925 pFN21AA0179 6925 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3183 4156 3183 Size: 973 5952 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4735 278 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4268 1937 Size: 2331 4594 Acc65I G'GTAC_C Cuts at: 4246 4246 Size: 6925 AclI AA'CG_TT Cuts at: 1563 5052 5425 1563 Size: 3489 373 3063 Fragments arranged by size: 3489 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2655 2976 3287 3863 3901 4148 4988 6372 Size: 1023 321 311 576 38 247 840 1384 Cuts at: 6372 6640 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1023 840 576 321 311 268 247 38 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6728 AflIII A'CryG_T Cuts at: 1203 3274 3438 5824 6801 1203 Size: 2071 164 2386 977 1327 Fragments arranged by size: 2386 2071 1327 977 164 AgeI A'CCGG_T Cuts at: 5756 5756 Size: 6925 AhdI GACnn_n'nnGTC Cuts at: 5654 5654 Size: 6925 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3674 1184 Size: 2490 4435 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3820 3852 3820 Size: 32 6893 AlwNI CAG_nnn'CTG Cuts at: 2050 3814 3856 3959 4668 6240 2050 Size: 1764 42 103 709 1572 2735 Fragments arranged by size: 2735 1764 1572 709 103 42 ApaI G_GGCC'C Cuts at: 2832 3397 3681 4079 2832 Size: 565 284 398 5678 Fragments arranged by size: 5678 565 398 284 ApaLI G'TGCA_C Cuts at: 4982 6138 4982 Size: 1156 5769 ApoI r'AATT_y Cuts at: 1493 2939 4034 4234 4460 4612 6721 1493 Size: 1446 1095 200 226 152 2109 1697 Fragments arranged by size: 2109 1697 1446 1095 226 200 152 AseI AT'TA_AT Cuts at: 160 1032 5479 160 Size: 872 4447 1606 Fragments arranged by size: 4447 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 AvaI C'yCGr_G Cuts at: 1870 1943 2589 3242 3388 3454 4083 4250 1870 Size: 73 646 653 146 66 629 167 4545 Fragments arranged by size: 4545 653 646 629 167 146 73 66 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4039 4072 4039 Size: 33 6892 BamHI G'GATC_C Cuts at: 4255 4622 4255 Size: 367 6558 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4246 5701 618 Size: 325 217 627 2459 1455 1842 Fragments arranged by size: 2459 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2832 3243 3389 3397 3505 3681 3696 Size: 781 660 411 146 8 108 176 15 Cuts at: 3696 4079 4244 1391 Size: 383 165 4072 Fragments arranged by size: 4072 781 660 411 383 176 165 146 108 15 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2123 2590 2663 2747 3128 4049 4136 Size: 971 224 467 73 84 381 921 87 Cuts at: 4136 928 Size: 3717 Fragments arranged by size: 3717 971 921 467 381 224 87 84 73 BbvCI CC'TCA_GC Cuts at: 2688 3857 2688 Size: 1169 5756 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3272 3306 5116 5150 1903 Size: 34 1335 34 1810 34 3678 Fragments arranged by size: 3678 1810 1335 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2722 4817 6027 2722 Size: 2095 1210 3620 Fragments arranged by size: 3620 2095 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6925 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3269 3459 3574 Size: 107 122 71 631 605 1597 190 115 Cuts at: 3574 5536 136 Size: 1962 1525 Fragments arranged by size: 1962 1597 1525 631 605 190 122 115 107 71 BglII A'GATC_T Cuts at: 6920 6920 Size: 6925 BlpI GC'TnA_GC Cuts at: 4342 4342 Size: 6925 Bme1580I G_kGCm'C Cuts at: 1790 2832 3397 3543 3681 4079 4986 6142 1790 Size: 1042 565 146 138 398 907 1156 2573 Fragments arranged by size: 2573 1156 1042 907 565 398 146 138 BmgBI CAC'GTC Cuts at: 1318 3303 1318 Size: 1985 4940 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2759 3321 4656 Size: 416 53 83 399 24 1342 562 1335 Cuts at: 4656 5614 442 Size: 958 1753 Fragments arranged by size: 1753 1342 1335 958 562 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6925 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2445 3343 3524 2445 Size: 898 181 5846 Fragments arranged by size: 5846 898 181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2822 3324 3710 3806 3936 5585 1239 Size: 1583 502 386 96 130 1649 2579 Fragments arranged by size: 2579 1649 1583 502 386 130 96 Bpu10I CC'TnA_GC Cuts at: 2688 3857 2688 Size: 1169 5756 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 2737 5050 5915 6213 2504 Size: 233 2313 865 298 3216 Fragments arranged by size: 3216 2313 865 298 233 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5588 882 Size: 617 4089 2219 Fragments arranged by size: 4089 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3275 4716 493 Size: 711 2071 1441 2702 Fragments arranged by size: 2702 2071 1441 711 BsaBI GATnn'nnATC Cuts at: 2514 6919 2514 Size: 4405 2520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 4732 5114 5749 Size: 53 83 186 936 521 2678 382 635 Cuts at: 5749 275 Size: 1451 Fragments arranged by size: 2678 1451 936 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5358 5756 6030 6177 6588 5358 Size: 398 274 147 411 5695 Fragments arranged by size: 5695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 3934 3964 1178 Size: 30 165 30 993 30 1508 30 4139 Fragments arranged by size: 4139 1508 993 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2705 3332 1186 Size: 1519 627 4779 Fragments arranged by size: 4779 1519 627 BseYI C'CCAG_C Cuts at: 2043 2358 3645 3707 3797 6128 6624 2043 Size: 315 1287 62 90 2331 496 2344 Fragments arranged by size: 2344 2331 1287 496 315 90 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3225 3312 1163 Size: 573 1489 87 4776 Fragments arranged by size: 4776 1489 573 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2028 4375 5136 6164 1540 Size: 275 186 27 2347 761 1028 2301 Fragments arranged by size: 2347 2301 1028 761 275 186 27 BsiHKAI G_wGCw'C Cuts at: 2172 3696 3774 4986 5071 6142 2172 Size: 1524 78 1212 85 1071 2955 Fragments arranged by size: 2955 1524 1212 1071 85 78 BsmI GAATG_Cn' Cuts at: 4436 4529 4436 Size: 93 6832 BsmBI CGTCTCn'nnnn_ Cuts at: 2633 2633 Size: 6925 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2832 3243 3389 3397 3505 3543 Size: 399 382 660 411 146 8 108 38 Cuts at: 3543 3681 3696 3774 4079 4244 4986 5071 6142 Size: 138 15 78 305 165 742 85 1071 Cuts at: 6142 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 660 411 399 382 305 165 146 138 108 85 78 38 15 8 BspEI T'CCGG_A Cuts at: 6588 6588 Size: 6925 BspHI T'CATG_A Cuts at: 4814 6544 4814 Size: 1730 5195 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3183 4156 4280 844 Size: 2339 973 124 3489 Fragments arranged by size: 3489 2339 973 124 BsrBI CCG'CTC Cuts at: 1394 1823 2084 4812 1394 Size: 429 261 2728 3507 Fragments arranged by size: 3507 2728 429 261 BsrDI GCAATG_nn' Cuts at: 65 1248 2181 3581 5420 5594 65 Size: 1183 933 1400 1839 174 1396 Fragments arranged by size: 1839 1400 1396 1183 933 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 3568 5569 5756 1700 Size: 110 213 1545 2001 187 2869 Fragments arranged by size: 2869 2001 1545 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6925 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6925 BssSI C'ACGA_G Cuts at: 4985 5997 4985 Size: 1012 5913 BstAPI GCAn_nnn'nTGC Cuts at: 794 3175 794 Size: 2381 4544 BstBI TT'CG_AA Cuts at: 2601 5729 2601 Size: 3128 3797 BstEII G'GTnAC_C Cuts at: 1184 2675 1184 Size: 1491 5434 BstXI CCAn_nnnn'nTGG Cuts at: 4213 4213 Size: 6925 BstYI r'GATC_y Cuts at: 1972 2031 4255 4622 5007 5024 6476 6562 6574 Size: 59 2224 367 385 17 1452 86 12 Cuts at: 6574 6920 1972 Size: 346 1977 Fragments arranged by size: 2224 1977 1452 385 367 346 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2324 3471 2324 Size: 1147 5778 BtgI C'CryG_G Cuts at: 513 1065 2004 3374 4071 4117 513 Size: 552 939 1370 697 46 3321 Fragments arranged by size: 3321 1370 939 697 552 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6186 Fragments arranged by size: 6186 683 56 BtsI GCAGTG_nn' Cuts at: 3426 3559 3829 3936 4441 5235 5255 3426 Size: 133 270 107 505 794 20 5096 Fragments arranged by size: 5096 794 505 270 133 107 20 ClaI AT'CG_AT Cuts at: 1554 2529 1554 Size: 975 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3535 3570 3747 3782 529 Size: 35 2971 35 177 35 3672 Fragments arranged by size: 3672 2971 177 35 35 35 DraI TTT'AAA Cuts at: 4229 4229 Size: 6925 DraIII CAC_nnn'GTG Cuts at: 4123 4123 Size: 6925 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5932 809 Size: 507 4616 1802 Fragments arranged by size: 4616 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 3279 3371 Size: 54 1425 50 275 18 195 1254 92 Cuts at: 3371 4167 4372 5261 6890 8 Size: 796 205 889 1629 43 Fragments arranged by size: 1629 1425 1254 889 796 275 205 195 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2025 4372 1537 Size: 275 213 2347 4090 Fragments arranged by size: 4090 2347 275 213 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 2631 2716 2791 3111 3377 3916 4855 Size: 807 471 85 75 320 266 539 939 Cuts at: 4855 1353 Size: 3423 Fragments arranged by size: 3423 939 807 539 471 320 266 85 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5512 5886 6032 387 Size: 1064 138 3923 374 146 1280 Fragments arranged by size: 3923 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2170 3694 2170 Size: 1524 5401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2655 2822 2976 3287 3324 3710 3806 Size: 393 1023 167 154 311 37 386 96 Cuts at: 3806 3863 3901 3936 4148 4988 5585 6372 6640 Size: 57 38 35 212 840 597 787 268 Cuts at: 6640 1239 Size: 1524 Fragments arranged by size: 1524 1023 840 787 597 393 386 311 268 212 167 154 96 57 38 37 35 EcoNI CCTnn'n_nnAGG Cuts at: 2399 3603 2399 Size: 1204 5721 EcoO109I rG'GnC_Cy Cuts at: 2407 2828 2829 3393 3476 3486 3596 3608 3677 Size: 421 1 564 83 10 110 12 69 Cuts at: 3677 3956 4076 2407 Size: 279 120 5256 Fragments arranged by size: 5256 564 421 279 120 110 83 69 12 10 1 EcoRI G'AATT_C Cuts at: 4034 4234 4612 4034 Size: 200 378 6347 Fragments arranged by size: 6347 378 200 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 4075 2401 Size: 1674 5251 FspI TGC'GCA Cuts at: 5431 5431 Size: 6925 HaeII r_GCGC'y Cuts at: 1678 2920 6072 1678 Size: 1242 3152 2531 Fragments arranged by size: 3152 2531 1242 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2411 2443 2462 2494 2572 Size: 32 135 32 839 32 19 32 78 Cuts at: 2572 2604 3287 3319 3425 3448 3457 3480 5566 Size: 32 683 32 106 23 9 23 2086 Cuts at: 5566 5598 5640 5672 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2086 839 683 135 106 78 42 32 32 32 32 32 32 32 32 23 23 19 9 HincII GTy'rAC Cuts at: 669 1657 1938 2206 4269 4515 669 Size: 988 281 268 2063 246 3079 Fragments arranged by size: 3079 2063 988 281 268 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6925 HpaI GTT'AAC Cuts at: 2206 4515 2206 Size: 2309 4616 KpnI G_GTAC'C Cuts at: 4250 4250 Size: 6925 MfeI C'AATT_G Cuts at: 4524 4524 Size: 6925 MluI A'CGCG_T Cuts at: 6801 6801 Size: 6925 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3390 6039 6223 1305 Size: 2085 2649 184 2007 Fragments arranged by size: 2649 2085 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4169 10 Size: 54 1425 343 2337 2766 Fragments arranged by size: 2766 2337 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 4883 5242 5401 518 Size: 648 945 2772 359 159 2042 Fragments arranged by size: 2772 2042 945 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2216 4341 5018 6166 6411 1606 Size: 441 169 2125 677 1148 245 2120 Fragments arranged by size: 2125 2120 1148 677 441 245 169 NaeI GCC'GGC Cuts at: 2025 3570 2025 Size: 1545 5380 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5434 Fragments arranged by size: 5434 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6925 NgoMIV G'CCGG_C Cuts at: 2023 3568 2023 Size: 1545 5380 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6925 NotI GC'GGCC_GC Cuts at: 4372 4372 Size: 6925 NspI r_CATG'y Cuts at: 1132 1229 3442 4283 5828 1132 Size: 97 2213 841 1545 2229 Fragments arranged by size: 2229 2213 1545 841 97 PasI CC'CwG_GG Cuts at: 1348 3197 4206 1348 Size: 1849 1009 4067 Fragments arranged by size: 4067 1849 1009 PciI A'CATG_T Cuts at: 3438 5824 3438 Size: 2386 4539 PflMI CCAn_nnn'nTGG Cuts at: 1108 2050 2572 3814 1108 Size: 942 522 1242 4219 Fragments arranged by size: 4219 1242 942 522 PfoI T'CCnGG_A Cuts at: 2073 2871 2073 Size: 798 6127 PmeI GTTT'AAAC Cuts at: 4229 4229 Size: 6925 PmlI CAC'GTG Cuts at: 3275 3275 Size: 6925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4994 5026 6507 6539 4994 Size: 32 1481 32 5380 Fragments arranged by size: 5380 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2407 3476 3486 2407 Size: 1069 10 5846 Fragments arranged by size: 5846 1069 10 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6925 PsiI TTA'TAA Cuts at: 4495 4495 Size: 6925 PspOMI G'GGCC_C Cuts at: 2828 3393 3677 4075 2828 Size: 565 284 398 5678 Fragments arranged by size: 5678 565 398 284 PstI C_TGCA'G Cuts at: 830 3180 3663 4277 830 Size: 2350 483 614 3478 Fragments arranged by size: 3478 2350 614 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6925 SacI G_AGCT'C Cuts at: 2172 3696 2172 Size: 1524 5401 SalI G'TCGA_C Cuts at: 1936 4267 1936 Size: 2331 4594 SanDI GG'GwC_CC Cuts at: 3486 3486 Size: 6925 SapI GCTCTTCn'nnn_ Cuts at: 2160 2716 3377 3916 2160 Size: 556 661 539 5169 Fragments arranged by size: 5169 661 556 539 SbfI CC_TGCA'GG Cuts at: 4277 4277 Size: 6925 ScaI AGT'ACT Cuts at: 5173 6885 5173 Size: 1712 5213 SfcI C'TryA_G Cuts at: 826 1045 2159 3176 3659 4273 5408 6089 6280 Size: 219 1114 1017 483 614 1135 681 191 Cuts at: 6280 826 Size: 1471 Fragments arranged by size: 1471 1135 1114 1017 681 614 483 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6925 SmaI CCC'GGG Cuts at: 1872 4252 1872 Size: 2380 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 2716 4083 5029 5930 6192 Size: 197 926 576 197 1367 946 901 262 Cuts at: 6192 820 Size: 1553 Fragments arranged by size: 1553 1367 946 926 901 576 262 197 197 SnaBI TAC'GTA Cuts at: 493 4716 493 Size: 4223 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6925 SphI G_CATG'C Cuts at: 1132 4283 1132 Size: 3151 3774 SspI AAT'ATT Cuts at: 5 52 4849 5 Size: 47 4797 2081 Fragments arranged by size: 4797 2081 47 StuI AGG'CCT Cuts at: 1517 3248 1517 Size: 1731 5194 StyI C'CwwG_G Cuts at: 513 1065 2004 4193 4364 513 Size: 552 939 2189 171 3074 Fragments arranged by size: 3074 2189 939 552 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2094 2657 3959 4952 4969 5122 5307 Size: 1255 514 563 1302 993 17 153 185 Cuts at: 5307 5752 325 Size: 445 1498 Fragments arranged by size: 1498 1302 1255 993 563 514 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 5171 6883 Size: 274 80 33 51 1443 126 3068 1712 Cuts at: 6883 96 Size: 138 Fragments arranged by size: 3068 1712 1443 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2178 2210 2396 2428 3934 3966 6885 6917 2178 Size: 32 186 32 1506 32 2919 32 2186 Fragments arranged by size: 2919 2186 1506 186 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6925 XbaI T'CTAG_A Cuts at: 4261 4261 Size: 6925 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4159 4159 Size: 6925 XhoI C'TCGA_G Cuts at: 1943 4083 1943 Size: 2140 4785 XmaI C'CCGG_G Cuts at: 1870 4250 1870 Size: 2380 4545 XmnI GAAnn'nnTTC Cuts at: 2154 5054 2154 Size: 2900 4025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4733 276 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI