(Circular) (Six-base) MAPSORT of: pFN21AA0099.seq Check: 2199 from: 1 to: 8191 pFN21AA0099 8191 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2880 6001 278 Size: 53 83 186 936 1344 3121 2468 Fragments arranged by size: 3121 2468 1344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3411 5534 1937 Size: 1474 2123 4594 Fragments arranged by size: 4594 2123 1474 Acc65I G'GTAC_C Cuts at: 3103 4446 5512 3103 Size: 1343 1066 5782 Fragments arranged by size: 5782 1343 1066 AclI AA'CG_TT Cuts at: 1563 6318 6691 1563 Size: 4755 373 3063 Fragments arranged by size: 4755 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3062 4320 4324 4749 6254 7638 7906 1632 Size: 1430 1258 4 425 1505 1384 268 1917 Fragments arranged by size: 1917 1505 1430 1384 1258 425 268 4 AfeI AGC'GCT Cuts at: 3304 3304 Size: 8191 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7994 AflIII A'CryG_T Cuts at: 1203 3058 5074 7090 8067 1203 Size: 1855 2016 2016 977 1327 Fragments arranged by size: 2016 2016 1855 1327 977 AgeI A'CCGG_T Cuts at: 4443 7022 4443 Size: 2579 5612 AhdI GACnn_n'nnGTC Cuts at: 6920 6920 Size: 8191 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3240 3456 5178 1184 Size: 2056 216 1722 4197 Fragments arranged by size: 4197 2056 1722 216 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3726 3758 3726 Size: 32 8159 AlwNI CAG_nnn'CTG Cuts at: 2532 3128 4298 4669 5934 7506 2532 Size: 596 1170 371 1265 1572 3217 Fragments arranged by size: 3217 1572 1265 1170 596 371 ApaI G_GGCC'C Cuts at: 5463 5463 Size: 8191 ApaLI G'TGCA_C Cuts at: 5236 6248 7404 5236 Size: 1012 1156 6023 Fragments arranged by size: 6023 1156 1012 ApoI r'AATT_y Cuts at: 1493 3607 4490 4646 5159 5500 5726 5878 7987 Size: 2114 883 156 513 341 226 152 2109 Cuts at: 7987 1493 Size: 1697 Fragments arranged by size: 2114 2109 1697 883 513 341 226 156 152 AseI AT'TA_AT Cuts at: 160 1032 4782 6745 160 Size: 872 3750 1963 1606 Fragments arranged by size: 3750 1963 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8191 AvaI C'yCGr_G Cuts at: 1870 1943 2692 4345 5516 1870 Size: 73 749 1653 1171 4545 Fragments arranged by size: 4545 1653 1171 749 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3087 3120 3087 Size: 33 8158 BamHI G'GATC_C Cuts at: 3580 3983 4226 5521 5888 3580 Size: 403 243 1295 367 5883 Fragments arranged by size: 5883 1295 403 367 243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2993 3103 3695 3971 4446 Size: 325 217 627 1206 110 592 276 475 Cuts at: 4446 5512 6967 618 Size: 1066 1455 1842 Fragments arranged by size: 1842 1455 1206 1066 627 592 475 325 276 217 110 BanII G_rGCy'C Cuts at: 1391 2687 3739 3920 5463 5510 1391 Size: 1296 1052 181 1543 47 4072 Fragments arranged by size: 4072 1543 1296 1052 181 47 BbeI G_GCGC'C Cuts at: 3975 3975 Size: 8191 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2587 4561 928 Size: 971 688 1974 4558 Fragments arranged by size: 4558 1974 971 688 BbvCI CC'TCA_GC Cuts at: 3781 3810 3781 Size: 29 8162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 6382 6416 1903 Size: 34 2012 34 2399 34 3678 Fragments arranged by size: 3678 2399 2012 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6083 7293 6083 Size: 1210 6981 BclI T'GATC_A Cuts at: 1548 2512 1548 Size: 964 7227 BfrBI ATG'CAT Cuts at: 4863 5356 4863 Size: 493 7698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6802 136 Size: 107 122 71 631 605 5130 1525 Fragments arranged by size: 5130 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8186 8186 Size: 8191 BlpI GC'TnA_GC Cuts at: 2968 3210 3495 4554 5608 2968 Size: 242 285 1059 1054 5551 Fragments arranged by size: 5551 1059 1054 285 242 Bme1580I G_kGCm'C Cuts at: 1790 3700 5240 5463 6252 7408 1790 Size: 1910 1540 223 789 1156 2573 Fragments arranged by size: 2573 1910 1540 1156 789 223 BmgBI CAC'GTC Cuts at: 1318 4699 5323 1318 Size: 3381 624 4186 Fragments arranged by size: 4186 3381 624 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2349 3758 4876 Size: 416 53 83 399 24 932 1409 1118 Cuts at: 4876 5922 6880 442 Size: 1046 958 1753 Fragments arranged by size: 1753 1409 1118 1046 958 932 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8191 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2456 3031 4371 2456 Size: 575 1340 6276 Fragments arranged by size: 6276 1340 575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 2435 4275 4315 4556 4568 4994 5102 Size: 934 262 1840 40 241 12 426 108 Cuts at: 5102 5444 6851 1239 Size: 342 1407 2579 Fragments arranged by size: 2579 1840 1407 934 426 342 262 241 108 40 12 Bpu10I CC'TnA_GC Cuts at: 3781 3810 4130 3781 Size: 29 320 7842 Fragments arranged by size: 7842 320 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2067 4772 6316 7181 7479 2067 Size: 2705 1544 865 298 2779 Fragments arranged by size: 2779 2705 1544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6854 882 Size: 617 5355 2219 Fragments arranged by size: 5355 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4849 5204 5302 5982 493 Size: 711 3645 355 98 680 2702 Fragments arranged by size: 3645 2702 711 680 355 98 BsaBI GATnn'nnATC Cuts at: 8185 8185 Size: 8191 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2877 3972 5998 6380 Size: 53 83 186 936 1344 1095 2026 382 Cuts at: 6380 7015 275 Size: 635 1451 Fragments arranged by size: 2026 1451 1344 1095 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3658 4443 6624 7022 7296 7443 7854 3658 Size: 785 2181 398 274 147 411 3995 Fragments arranged by size: 3995 2181 785 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2663 2693 3726 3756 4189 Size: 30 165 30 1260 30 1033 30 433 Cuts at: 4189 4219 1178 Size: 30 5150 Fragments arranged by size: 5150 1260 1033 433 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2304 2316 2831 3540 4132 5027 1186 Size: 1118 12 515 709 592 895 4350 Fragments arranged by size: 4350 1118 895 709 592 515 12 BseYI C'CCAG_C Cuts at: 2177 2559 2973 3364 3816 3822 3924 7394 7890 Size: 382 414 391 452 6 102 3470 496 Cuts at: 7890 2177 Size: 2478 Fragments arranged by size: 3470 2478 496 452 414 391 382 102 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 2876 3771 1163 Size: 573 858 282 895 5583 Fragments arranged by size: 5583 895 858 573 282 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2639 5092 5641 6402 7430 1540 Size: 275 186 638 2453 549 761 1028 2301 Fragments arranged by size: 2453 2301 1028 761 638 549 275 186 BsiHKAI G_wGCw'C Cuts at: 2687 3739 4980 5088 5223 5240 6252 6337 7408 Size: 1052 1241 108 135 17 1012 85 1071 Cuts at: 7408 2687 Size: 3470 Fragments arranged by size: 3470 1241 1071 1052 1012 135 108 85 17 BsiWI C'GTAC_G Cuts at: 5204 5204 Size: 8191 BsmI GAATG_Cn' Cuts at: 3715 5702 5795 3715 Size: 1987 93 6111 Fragments arranged by size: 6111 1987 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2687 3700 3739 3920 4980 5088 5223 Size: 399 897 1013 39 181 1060 108 135 Cuts at: 5223 5240 5463 5510 6252 6337 7408 1391 Size: 17 223 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 1060 1013 897 742 399 223 181 135 108 85 47 39 17 BspEI T'CCGG_A Cuts at: 7854 7854 Size: 8191 BspHI T'CATG_A Cuts at: 2392 6080 7810 2392 Size: 3688 1730 2773 Fragments arranged by size: 3688 2773 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3091 3248 3502 4765 5546 844 Size: 2247 157 254 1263 781 3489 Fragments arranged by size: 3489 2247 1263 781 254 157 BsrBI CCG'CTC Cuts at: 1394 1823 6078 1394 Size: 429 4255 3507 Fragments arranged by size: 4255 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5140 5176 6686 6860 65 Size: 1183 3892 36 1510 174 1396 Fragments arranged by size: 3892 1510 1396 1183 174 36 BsrFI r'CCGG_y Cuts at: 1700 1810 4443 6835 7022 1700 Size: 110 2633 2392 187 2869 Fragments arranged by size: 2869 2633 2392 187 110 BsrGI T'GTAC_A Cuts at: 96 4873 5041 96 Size: 4777 168 3246 Fragments arranged by size: 4777 3246 168 BssHII G'CGCG_C Cuts at: 1925 5212 1925 Size: 3287 4904 BssSI C'ACGA_G Cuts at: 6251 7263 6251 Size: 1012 7179 BstAPI GCAn_nnn'nTGC Cuts at: 794 2443 794 Size: 1649 6542 BstBI TT'CG_AA Cuts at: 4435 6995 4435 Size: 2560 5631 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8191 BstXI CCAn_nnnn'nTGG Cuts at: 3004 3389 5302 3004 Size: 385 1913 5893 Fragments arranged by size: 5893 1913 385 BstYI r'GATC_y Cuts at: 1972 2204 2932 3580 3983 4226 5361 5521 5888 Size: 232 728 648 403 243 1135 160 367 Cuts at: 5888 6273 6290 7742 7828 7840 8186 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1135 728 648 403 385 367 346 243 232 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5097 5097 Size: 8191 BtgI C'CryG_G Cuts at: 513 1065 2376 3051 3520 4155 513 Size: 552 1311 675 469 635 4549 Fragments arranged by size: 4549 1311 675 635 552 469 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2441 3238 5359 487 Size: 683 56 1215 797 2121 3319 Fragments arranged by size: 3319 2121 1215 797 683 56 BtsI GCAGTG_nn' Cuts at: 2043 3609 4039 4072 4273 4321 5707 6501 6521 Size: 1566 430 33 201 48 1386 794 20 Cuts at: 6521 2043 Size: 3713 Fragments arranged by size: 3713 1566 1386 794 430 201 48 33 20 ClaI AT'CG_AT Cuts at: 1554 2329 4902 5226 1554 Size: 775 2573 324 4519 Fragments arranged by size: 4519 2573 775 324 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8156 DraI TTT'AAA Cuts at: 5495 5495 Size: 8191 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7198 809 Size: 507 5882 1802 Fragments arranged by size: 5882 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4807 5415 5638 Size: 54 1425 50 275 18 2977 608 223 Cuts at: 5638 6527 8156 8 Size: 889 1629 43 Fragments arranged by size: 2977 1629 1425 889 608 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5638 1537 Size: 275 3826 4090 Fragments arranged by size: 4090 3826 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2416 3081 3911 4222 4237 4343 4369 Size: 668 395 665 830 311 15 106 26 Cuts at: 4369 5388 6121 1353 Size: 1019 733 3423 Fragments arranged by size: 3423 1019 830 733 668 665 395 311 106 26 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3976 5342 6778 7152 7298 387 Size: 1064 138 2387 1366 1436 374 146 1280 Fragments arranged by size: 2387 1436 1366 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2685 3737 2685 Size: 1052 7139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2435 3062 4275 4315 4320 4324 Size: 393 541 262 627 1213 40 5 4 Cuts at: 4324 4556 4568 4749 4994 5102 5444 6254 6851 Size: 232 12 181 245 108 342 810 597 Cuts at: 6851 7638 7906 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1213 810 787 627 597 541 393 342 268 262 245 232 181 108 40 12 5 4 EcoNI CCTnn'n_nnAGG Cuts at: 2199 2199 Size: 8191 EcoO109I rG'GnC_Cy Cuts at: 5459 5459 Size: 8191 EcoRI G'AATT_C Cuts at: 3607 5500 5878 3607 Size: 1893 378 5920 Fragments arranged by size: 5920 1893 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2057 2951 2057 Size: 894 7297 FspI TGC'GCA Cuts at: 6697 6697 Size: 8191 HaeII r_GCGC'y Cuts at: 1678 3306 3975 4299 4329 4561 7338 1678 Size: 1628 669 324 30 232 2777 2531 Fragments arranged by size: 2777 2531 1628 669 324 232 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3193 3225 3712 3744 4189 Size: 32 135 32 1621 32 487 32 445 Cuts at: 4189 4221 4683 4715 6832 6864 6906 6938 1373 Size: 32 462 32 2117 32 42 32 2626 Fragments arranged by size: 2626 2117 1621 487 462 445 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 5452 5535 5781 669 Size: 988 281 1263 2251 83 246 3079 Fragments arranged by size: 3079 2251 1263 988 281 246 83 HindIII A'AGCT_T Cuts at: 748 4237 748 Size: 3489 4702 HpaI GTT'AAC Cuts at: 5781 5781 Size: 8191 KasI G'GCGC_C Cuts at: 3971 3971 Size: 8191 KpnI G_GTAC'C Cuts at: 3107 4450 5516 3107 Size: 1343 1066 5782 Fragments arranged by size: 5782 1343 1066 MfeI C'AATT_G Cuts at: 5790 5790 Size: 8191 MluI A'CGCG_T Cuts at: 8067 8067 Size: 8191 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 4047 5095 7305 7489 1305 Size: 1737 1005 1048 2210 184 2007 Fragments arranged by size: 2210 2007 1737 1048 1005 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4809 5417 10 Size: 54 1425 343 2977 608 2784 Fragments arranged by size: 2977 2784 1425 608 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4944 5276 6149 6508 6667 518 Size: 648 3778 332 873 359 159 2042 Fragments arranged by size: 3778 2042 873 648 359 332 159 MspA1I CmG'CkG Cuts at: 1606 2156 2177 2977 3163 3206 3236 3272 3335 Size: 550 21 800 186 43 30 36 63 Cuts at: 3335 3368 3446 3452 3596 3785 4528 4553 5341 Size: 33 78 6 144 189 743 25 788 Cuts at: 5341 5607 6284 7432 7677 1606 Size: 266 677 1148 245 2120 Fragments arranged by size: 2120 1148 800 788 743 677 550 266 245 189 186 144 78 63 43 36 33 30 25 21 6 NarI GG'CG_CC Cuts at: 3972 3972 Size: 8191 NcoI C'CATG_G Cuts at: 513 1065 2376 3051 513 Size: 552 1311 675 5653 Fragments arranged by size: 5653 1311 675 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8191 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8191 NotI GC'GGCC_GC Cuts at: 5638 5638 Size: 8191 NsiI A_TGCA'T Cuts at: 4865 5358 4865 Size: 493 7698 NspI r_CATG'y Cuts at: 1132 1229 2019 2107 3062 3649 4165 5078 5549 Size: 97 790 88 955 587 516 913 471 Cuts at: 5549 7094 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 955 913 790 587 516 471 97 88 PasI CC'CwG_GG Cuts at: 1348 3314 3402 3913 3979 1348 Size: 1966 88 511 66 5560 Fragments arranged by size: 5560 1966 511 88 66 PciI A'CATG_T Cuts at: 3058 5074 7090 3058 Size: 2016 2016 4159 Fragments arranged by size: 4159 2016 2016 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8191 PfoI T'CCnGG_A Cuts at: 4147 4971 4147 Size: 824 7367 PmeI GTTT'AAAC Cuts at: 5495 5495 Size: 8191 PmlI CAC'GTG Cuts at: 4849 4849 Size: 8191 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3726 3758 6260 6292 7773 7805 3726 Size: 32 2502 32 1481 32 4112 Fragments arranged by size: 4112 2502 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8191 PsiI TTA'TAA Cuts at: 4077 4122 5761 4077 Size: 45 1639 6507 Fragments arranged by size: 6507 1639 45 PspOMI G'GGCC_C Cuts at: 5459 5459 Size: 8191 PstI C_TGCA'G Cuts at: 830 3349 3457 3601 5543 830 Size: 2519 108 144 1942 3478 Fragments arranged by size: 3478 2519 1942 144 108 PvuI CG_AT'CG Cuts at: 2001 2639 2001 Size: 638 7553 PvuII CAG'CTG Cuts at: 2156 2177 2977 3163 3206 3236 3335 3368 3596 Size: 21 800 186 43 30 99 33 228 Cuts at: 3596 3785 4528 4553 2156 Size: 189 743 25 5794 Fragments arranged by size: 5794 800 743 228 189 186 99 43 33 30 25 21 SacI G_AGCT'C Cuts at: 2687 3739 2687 Size: 1052 7139 SalI G'TCGA_C Cuts at: 1936 5533 1936 Size: 3597 4594 SapI GCTCTTCn'nnn_ Cuts at: 2416 2416 Size: 8191 SbfI CC_TGCA'GG Cuts at: 5543 5543 Size: 8191 ScaI AGT'ACT Cuts at: 5432 6439 8151 5432 Size: 1007 1712 5472 Fragments arranged by size: 5472 1712 1007 SexAI A'CCwGG_T Cuts at: 2584 2584 Size: 8191 SfcI C'TryA_G Cuts at: 826 1045 2208 3345 3453 3597 4715 5539 6674 Size: 219 1163 1137 108 144 1118 824 1135 Cuts at: 6674 7355 7546 826 Size: 681 191 1471 Fragments arranged by size: 1471 1163 1137 1135 1118 824 681 219 191 144 108 SfoI GGC'GCC Cuts at: 3973 3973 Size: 8191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8191 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8191 SmaI CCC'GGG Cuts at: 1872 2694 5518 1872 Size: 822 2824 4545 Fragments arranged by size: 4545 2824 822 SmlI C'TyrA_G Cuts at: 820 1017 1943 2082 4751 6295 7196 7458 820 Size: 197 926 139 2669 1544 901 262 1553 Fragments arranged by size: 2669 1553 1544 926 901 262 197 139 SnaBI TAC'GTA Cuts at: 493 5982 493 Size: 5489 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8191 SphI G_CATG'C Cuts at: 1132 3649 4165 5549 1132 Size: 2517 516 1384 3774 Fragments arranged by size: 3774 2517 1384 516 SspI AAT'ATT Cuts at: 5 52 2502 6115 5 Size: 47 2450 3613 2081 Fragments arranged by size: 3613 2450 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8191 StyI C'CwwG_G Cuts at: 513 1065 2376 2729 3042 3051 3942 3991 5630 Size: 552 1311 353 313 9 891 49 1639 Cuts at: 5630 513 Size: 3074 Fragments arranged by size: 3074 1639 1311 891 552 353 313 49 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 6218 6235 6388 6573 7018 325 Size: 1255 918 3720 17 153 185 445 1498 Fragments arranged by size: 3720 1498 1255 918 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2623 4873 5041 Size: 274 80 33 51 1443 646 2250 168 Cuts at: 5041 5077 5137 5391 5430 6437 8149 96 Size: 36 60 254 39 1007 1712 138 Fragments arranged by size: 2250 1712 1443 1007 646 274 254 168 138 80 60 51 39 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8151 8183 8151 Size: 32 8159 Tth111I GACn'n_nGTC Cuts at: 1316 2586 3630 1316 Size: 1270 1044 5877 Fragments arranged by size: 5877 1270 1044 XbaI T'CTAG_A Cuts at: 5527 5527 Size: 8191 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3058 3841 3940 4505 3058 Size: 783 99 565 6744 Fragments arranged by size: 6744 783 565 99 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8191 XmaI C'CCGG_G Cuts at: 1870 2692 5516 1870 Size: 822 2824 4545 Fragments arranged by size: 4545 2824 822 XmnI GAAnn'nnTTC Cuts at: 4431 4686 6320 4431 Size: 255 1634 6302 Fragments arranged by size: 6302 1634 255 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2878 5999 276 Size: 53 83 186 936 1344 3121 2468 Fragments arranged by size: 3121 2468 1344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI