Restriction map for
pFN21AA0066
(Circular) (Six-base) MAPSORT of: pFN21AA0066.seq Check: 4385 from: 1 to: 7648
pFN21AA0066 7648 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4907 4907
Size: 7648
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5458 278
Size: 53 83 186 936 3922 2468
Fragments arranged by size:
3922 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4991 1937
Size: 3054 4594
Acc65I G'GTAC_C
Cuts at: 4969 4969
Size: 7648
AclI AA'CG_TT
Cuts at: 1563 5775 6148 1563
Size: 4212 373 3063
Fragments arranged by size:
4212 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3603 3801 3990 4395 4659 5711 7095 7363
Size: 1971 198 189 405 264 1052 1384 268
Cuts at: 7363 1632
Size: 1917
Fragments arranged by size:
1971 1917 1384 1052 405 268 264 198
189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7451
AflIII A'CryG_T
Cuts at: 1203 6547 7524 1203
Size: 5344 977 1327
Fragments arranged by size:
5344 1327 977
AgeI A'CCGG_T
Cuts at: 4652 6479 4652
Size: 1827 5821
AhdI GACnn_n'nnGTC
Cuts at: 6377 6377
Size: 7648
AleI CACnn'nnGTG
Cuts at: 1166 3189 4835 1166
Size: 2023 1646 3979
Fragments arranged by size:
3979 2023 1646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4178 4853 1184
Size: 2994 675 3979
Fragments arranged by size:
3979 2994 675
AlwNI CAG_nnn'CTG
Cuts at: 5391 6963 5391
Size: 1572 6076
ApaLI G'TGCA_C
Cuts at: 5705 6861 5705
Size: 1156 6492
ApoI r'AATT_y
Cuts at: 1493 2196 2508 2993 3003 3452 3755 3841 4817
Size: 703 312 485 10 449 303 86 976
Cuts at: 4817 4957 5183 5335 7444 1493
Size: 140 226 152 2109 1697
Fragments arranged by size:
2109 1697 976 703 485 449 312 303
226 152 140 86 10
AseI AT'TA_AT
Cuts at: 160 1032 6202 160
Size: 872 5170 1606
Fragments arranged by size:
5170 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7648
AvaI C'yCGr_G
Cuts at: 1870 1943 4116 4973 1870
Size: 73 2173 857 4545
Fragments arranged by size:
4545 2173 857 73
BamHI G'GATC_C
Cuts at: 2911 4978 5345 2911
Size: 2067 367 5214
Fragments arranged by size:
5214 2067 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2491 2813 3673 4338 4919
Size: 325 217 627 704 322 860 665 581
Cuts at: 4919 4969 6424 618
Size: 50 1455 1842
Fragments arranged by size:
1842 1455 860 704 665 627 581 325
322 217 50
BanII G_rGCy'C
Cuts at: 1391 2025 4009 4543 4760 4967 1391
Size: 634 1984 534 217 207 4072
Fragments arranged by size:
4072 1984 634 534 217 207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3330 4485 928
Size: 971 1431 1155 4091
Fragments arranged by size:
4091 1431 1155 971
BbvCI CC'TCA_GC
Cuts at: 2739 4872 2739
Size: 2133 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5839 5873 1903
Size: 34 3902 34 3678
Fragments arranged by size:
3902 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5540 6750 5540
Size: 1210 6438
BclI T'GATC_A
Cuts at: 1548 3402 1548
Size: 1854 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4712 6259 136
Size: 107 122 71 631 605 3040 1547 1525
Fragments arranged by size:
3040 1547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3523 3949 4600 7643 3523
Size: 426 651 3043 3528
Fragments arranged by size:
3528 3043 651 426
BlpI GC'TnA_GC
Cuts at: 4233 5065 4233
Size: 832 6816
Bme1580I G_kGCm'C
Cuts at: 1790 4186 5709 6865 1790
Size: 2396 1523 1156 2573
Fragments arranged by size:
2573 2396 1523 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7648
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5379 6337 442
Size: 416 53 83 399 24 3962 958 1753
Fragments arranged by size:
3962 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7648
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3923
Size: 7648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2661 2733 3757 4649 6308 1239
Size: 1422 72 1024 892 1659 2579
Fragments arranged by size:
2579 1659 1422 1024 892 72
Bpu10I CC'TnA_GC
Cuts at: 2739 4872 2739
Size: 2133 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4358 5773 6638 6936 4358
Size: 1415 865 298 5070
Fragments arranged by size:
5070 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3657 6311 882
Size: 617 2158 2654 2219
Fragments arranged by size:
2654 2219 2158 617
BsaAI yAC'GTr
Cuts at: 493 1204 2378 3593 5439 493
Size: 711 1174 1215 1846 2702
Fragments arranged by size:
2702 1846 1215 1174 711
BsaBI GATnn'nnATC
Cuts at: 2910 7642 2910
Size: 4732 2916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5455 5837 6472 275
Size: 53 83 186 936 3922 382 635 1451
Fragments arranged by size:
3922 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4652 6081 6479 6753 6900 7311 4652
Size: 1429 398 274 147 411 4989
Fragments arranged by size:
4989 1429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2763 2793 1178
Size: 30 165 30 1360 30 6033
Fragments arranged by size:
6033 1360 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2787 3212 4148 4601 4751 1186
Size: 1601 425 936 453 150 4083
Fragments arranged by size:
4083 1601 936 453 425 150
BseYI C'CCAG_C
Cuts at: 2317 6851 7347 2317
Size: 4534 496 2618
Fragments arranged by size:
4534 2618 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2388 3339 4283 4677 1163
Size: 573 652 951 944 394 4134
Fragments arranged by size:
4134 951 944 652 573 394
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5098 5859 6887 1540
Size: 275 186 3097 761 1028 2301
Fragments arranged by size:
3097 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2350 4021 4543 5709 5794 6865 2350
Size: 1671 522 1166 85 1071 3133
Fragments arranged by size:
3133 1671 1166 1071 522 85
BsmI GAATG_Cn'
Cuts at: 3770 4708 5159 5252 3770
Size: 938 451 93 6166
Fragments arranged by size:
6166 938 451 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2350 4009 4021 4186 4543 4760
Size: 399 235 325 1659 12 165 357 217
Cuts at: 4760 4967 5709 5794 6865 1391
Size: 207 742 85 1071 2174
Fragments arranged by size:
2174 1659 1071 742 399 357 325 235
217 207 165 85 12
BspEI T'CCGG_A
Cuts at: 7311 7311
Size: 7648
BspHI T'CATG_A
Cuts at: 5537 7267 5537
Size: 1730 5918
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4330 4907 5003 844
Size: 3486 577 96 3489
Fragments arranged by size:
3489 3486 577 96
BsrBI CCG'CTC
Cuts at: 1394 1823 5535 1394
Size: 429 3712 3507
Fragments arranged by size:
3712 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2350 2669 4162 4530 6143 6317 65
Size: 1183 1102 319 1493 368 1613 174 1396
Fragments arranged by size:
1613 1493 1396 1183 1102 368 319 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3112 4652 6292 6479 1700
Size: 110 1302 1540 1640 187 2869
Fragments arranged by size:
2869 1640 1540 1302 187 110
BsrGI T'GTAC_A
Cuts at: 96 2242 4881 96
Size: 2146 2639 2863
Fragments arranged by size:
2863 2639 2146
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7648
BssSI C'ACGA_G
Cuts at: 5708 6720 5708
Size: 1012 6636
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 4331 794
Size: 1520 2017 4111
Fragments arranged by size:
4111 2017 1520
BstBI TT'CG_AA
Cuts at: 2574 2947 6452 2574
Size: 373 3505 3770
Fragments arranged by size:
3770 3505 373
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7648
BstYI r'GATC_y
Cuts at: 1972 2824 2911 3523 3949 4600 4978 5345 5730
Size: 852 87 612 426 651 378 367 385
Cuts at: 5730 5747 7199 7285 7297 7643 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 852 651 612 426 385 378
367 346 87 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3828 3828
Size: 7648
BtgI C'CryG_G
Cuts at: 513 1065 2004 3415 513
Size: 552 939 1411 4746
Fragments arranged by size:
4746 1411 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6909
Fragments arranged by size:
6909 683 56
BtsI GCAGTG_nn'
Cuts at: 2058 3159 3884 5164 5958 5978 2058
Size: 1101 725 1280 794 20 3728
Fragments arranged by size:
3728 1280 1101 794 725 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7648
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7613
DraI TTT'AAA
Cuts at: 3001 4952 3001
Size: 1951 5697
DraIII CAC_nnn'GTG
Cuts at: 2411 2411
Size: 7648
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6655 809
Size: 507 5339 1802
Fragments arranged by size:
5339 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4810 5095 5984
Size: 54 1425 50 275 18 2980 285 889
Cuts at: 5984 7613 8
Size: 1629 43
Fragments arranged by size:
2980 1629 1425 889 285 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5095 1537
Size: 275 3283 4090
Fragments arranged by size:
4090 3283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 3954 4125 4657 4742 5578 1353
Size: 822 1779 171 532 85 836 3423
Fragments arranged by size:
3423 1779 836 822 532 171 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6235 6609 6755 387
Size: 1064 138 4646 374 146 1280
Fragments arranged by size:
4646 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4541 4541
Size: 7648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2661 2733 3603 3757 3801 3990 4395
Size: 393 1029 72 870 154 44 189 405
Cuts at: 4395 4649 4659 5711 6308 7095 7363 1239
Size: 254 10 1052 597 787 268 1524
Fragments arranged by size:
1524 1052 1029 870 787 597 405 393
268 254 189 154 72 44 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3446 3446
Size: 7648
EcoO109I rG'GnC_Cy
Cuts at: 2552 3824 2552
Size: 1272 6376
EcoRI G'AATT_C
Cuts at: 4817 4957 5335 4817
Size: 140 378 7130
Fragments arranged by size:
7130 378 140
EcoRV GAT'ATC
Cuts at: 2635 2635
Size: 7648
FspI TGC'GCA
Cuts at: 4829 6154 4829
Size: 1325 6323
HaeII r_GCGC'y
Cuts at: 1678 6795 1678
Size: 5117 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2641 2673 3101
Size: 32 135 32 440 32 597 32 428
Cuts at: 3101 3133 3934 3966 4021 4040 4053 4072 6289
Size: 32 801 32 55 19 13 19 2217
Cuts at: 6289 6321 6363 6395 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2217 801 597 440 428 135 55
42 32 32 32 32 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 4992 5238 669
Size: 988 281 1086 1968 246 3079
Fragments arranged by size:
3079 1968 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7648
HpaI GTT'AAC
Cuts at: 5238 5238
Size: 7648
KpnI G_GTAC'C
Cuts at: 4973 4973
Size: 7648
MfeI C'AATT_G
Cuts at: 5247 5247
Size: 7648
MluI A'CGCG_T
Cuts at: 7524 7524
Size: 7648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2761 6762 6946 1305
Size: 1456 4001 184 2007
Fragments arranged by size:
4001 2007 1456 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5826
Fragments arranged by size:
5826 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3189 4835 5606 5965 6124 518
Size: 648 2023 1646 771 359 159 2042
Fragments arranged by size:
2042 2023 1646 771 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4367 4876 5064 5741 6889 7134 1606
Size: 2761 509 188 677 1148 245 2120
Fragments arranged by size:
2761 2120 1148 677 509 245 188
NaeI GCC'GGC
Cuts at: 3114 3114
Size: 7648
NcoI C'CATG_G
Cuts at: 513 1065 2004 3415 513
Size: 552 939 1411 4746
Fragments arranged by size:
4746 1411 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7648
NgoMIV G'CCGG_C
Cuts at: 3112 3112
Size: 7648
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7648
NotI GC'GGCC_GC
Cuts at: 5095 5095
Size: 7648
NspI r_CATG'y
Cuts at: 1132 1229 4027 4673 5006 6551 1132
Size: 97 2798 646 333 1545 2229
Fragments arranged by size:
2798 2229 1545 646 333 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7648
PciI A'CATG_T
Cuts at: 6547 6547
Size: 7648
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3278 1108
Size: 2170 5478
PfoI T'CCnGG_A
Cuts at: 3499 3734 3499
Size: 235 7413
PmeI GTTT'AAAC
Cuts at: 4952 4952
Size: 7648
PmlI CAC'GTG
Cuts at: 3593 3593
Size: 7648
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5717 5749 7230 7262 5717
Size: 32 1481 32 6103
Fragments arranged by size:
6103 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2552 3824 2552
Size: 1272 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7648
PsiI TTA'TAA
Cuts at: 5218 5218
Size: 7648
PstI C_TGCA'G
Cuts at: 830 4327 4720 5000 830
Size: 3497 393 280 3478
Fragments arranged by size:
3497 3478 393 280
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7648
PvuII CAG'CTG
Cuts at: 4367 4367
Size: 7648
SacI G_AGCT'C
Cuts at: 4543 4543
Size: 7648
SalI G'TCGA_C
Cuts at: 1936 4990 1936
Size: 3054 4594
SapI GCTCTTCn'nnn_
Cuts at: 3954 3954
Size: 7648
SbfI CC_TGCA'GG
Cuts at: 5000 5000
Size: 7648
ScaI AGT'ACT
Cuts at: 5896 7608 5896
Size: 1712 5936
SfcI C'TryA_G
Cuts at: 826 1045 3554 4323 4716 4996 6131 6812 7003
Size: 219 2509 769 393 280 1135 681 191
Cuts at: 7003 826
Size: 1471
Fragments arranged by size:
2509 1471 1135 769 681 393 280 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7648
SgrAI Cr'CCGG_yG
Cuts at: 1700 4652 1700
Size: 2952 4696
SmaI CCC'GGG
Cuts at: 1872 4118 4975 1872
Size: 2246 857 4545
Fragments arranged by size:
4545 2246 857
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4373 5752 6653 6915 820
Size: 197 926 2430 1379 901 262 1553
Fragments arranged by size:
2430 1553 1379 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5439 493
Size: 4946 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7648
SphI G_CATG'C
Cuts at: 1132 4027 5006 1132
Size: 2895 979 3774
Fragments arranged by size:
3774 2895 979
SspI AAT'ATT
Cuts at: 5 52 5572 5
Size: 47 5520 2081
Fragments arranged by size:
5520 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7648
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 3415 5087 513
Size: 552 939 465 946 1672 3074
Fragments arranged by size:
3074 1672 946 939 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2885 4533 5675 5692 5845 6030 6475
Size: 1255 1305 1648 1142 17 153 185 445
Cuts at: 6475 325
Size: 1498
Fragments arranged by size:
1648 1498 1305 1255 1142 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2242 2607 2707
Size: 274 80 33 51 1443 265 365 100
Cuts at: 2707 4369 4881 5894 7606 96
Size: 1662 512 1013 1712 138
Fragments arranged by size:
1712 1662 1443 1013 512 365 274 265
138 100 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4870 4902 7608 7640 4870
Size: 32 2706 32 4878
Fragments arranged by size:
4878 2706 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7648
XbaI T'CTAG_A
Cuts at: 4984 4984
Size: 7648
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4206 4206
Size: 7648
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7648
XmaI C'CCGG_G
Cuts at: 1870 4116 4973 1870
Size: 2246 857 4545
Fragments arranged by size:
4545 2246 857
XmnI GAAnn'nnTTC
Cuts at: 2580 3286 3456 4500 5777 2580
Size: 706 170 1044 1277 4451
Fragments arranged by size:
4451 1277 1044 706 170
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5456 276
Size: 53 83 186 936 3922 2468
Fragments arranged by size:
3922 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI