(Circular) (Six-base) MAPSORT of: pFN21AA0055.seq Check: 2736 from: 1 to: 8059 pFN21AA0055 8059 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2727 3070 5049 2727 Size: 343 1979 5737 Fragments arranged by size: 5737 1979 343 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5869 278 Size: 53 83 186 936 4333 2468 Fragments arranged by size: 4333 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2936 5402 1937 Size: 999 2466 4594 Fragments arranged by size: 4594 2466 999 Acc65I G'GTAC_C Cuts at: 5380 5380 Size: 8059 AclI AA'CG_TT Cuts at: 1563 5041 6186 6559 1563 Size: 3478 1145 373 3063 Fragments arranged by size: 3478 3063 1145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2004 2147 2373 2691 3279 3552 3945 4163 Size: 372 143 226 318 588 273 393 218 Cuts at: 4163 5283 6122 7506 7774 1632 Size: 1120 839 1384 268 1917 Fragments arranged by size: 1917 1384 1120 839 588 393 372 318 273 268 226 218 143 AfeI AGC'GCT Cuts at: 4148 4148 Size: 8059 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7862 AflIII A'CryG_T Cuts at: 1203 4244 4817 6958 7935 1203 Size: 3041 573 2141 977 1327 Fragments arranged by size: 3041 2141 1327 977 573 AgeI A'CCGG_T Cuts at: 6890 6890 Size: 8059 AhdI GACnn_n'nnGTC Cuts at: 6788 6788 Size: 8059 AleI CACnn'nnGTG Cuts at: 1166 5184 1166 Size: 4018 4041 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2167 1184 Size: 983 7076 AlwNI CAG_nnn'CTG Cuts at: 3158 3410 5802 7374 3158 Size: 252 2392 1572 3843 Fragments arranged by size: 3843 2392 1572 252 ApaLI G'TGCA_C Cuts at: 2114 6116 7272 2114 Size: 4002 1156 2901 Fragments arranged by size: 4002 2901 1156 ApoI r'AATT_y Cuts at: 1493 3840 4456 4490 5368 5594 5746 7855 1493 Size: 2347 616 34 878 226 152 2109 1697 Fragments arranged by size: 2347 2109 1697 878 616 226 152 34 AseI AT'TA_AT Cuts at: 160 1032 6613 160 Size: 872 5581 1606 Fragments arranged by size: 5581 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8059 AvaI C'yCGr_G Cuts at: 1870 1943 5384 1870 Size: 73 3441 4545 Fragments arranged by size: 4545 3441 73 BamHI G'GATC_C Cuts at: 5389 5756 5389 Size: 367 7692 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5380 6835 618 Size: 325 217 627 3593 1455 1842 Fragments arranged by size: 3593 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4975 5378 1391 Size: 3584 403 4072 Fragments arranged by size: 4072 3584 403 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2078 928 Size: 971 179 6909 Fragments arranged by size: 6909 971 179 BbvCI CC'TCA_GC Cuts at: 3625 3625 Size: 8059 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6250 6284 1903 Size: 34 50 34 4229 34 3678 Fragments arranged by size: 4229 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3862 4586 5951 7161 3862 Size: 724 1365 1210 4760 Fragments arranged by size: 4760 1365 1210 724 BclI T'GATC_A Cuts at: 1548 4699 5257 1548 Size: 3151 558 4350 Fragments arranged by size: 4350 3151 558 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6670 136 Size: 107 122 71 631 605 4998 1525 Fragments arranged by size: 4998 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2130 4263 4651 8054 2130 Size: 2133 388 3403 2135 Fragments arranged by size: 3403 2135 2133 388 BlpI GC'TnA_GC Cuts at: 4116 5476 4116 Size: 1360 6699 Bme1580I G_kGCm'C Cuts at: 1790 2118 2125 3771 6120 7276 1790 Size: 328 7 1646 2349 1156 2573 Fragments arranged by size: 2573 2349 1646 1156 328 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8059 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2926 5790 6748 Size: 416 53 83 399 24 1509 2864 958 Cuts at: 6748 442 Size: 1753 Fragments arranged by size: 2864 1753 1509 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8059 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3126 6719 1239 Size: 1887 3593 2579 Fragments arranged by size: 3593 2579 1887 Bpu10I CC'TnA_GC Cuts at: 2949 3625 2949 Size: 676 7383 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2390 4252 6184 7049 7347 2390 Size: 1862 1932 865 298 3102 Fragments arranged by size: 3102 1932 1862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2525 4646 6722 882 Size: 617 1026 2121 2076 2219 Fragments arranged by size: 2219 2121 2076 1026 617 BsaAI yAC'GTr Cuts at: 493 1204 2203 5850 493 Size: 711 999 3647 2702 Fragments arranged by size: 3647 2702 999 711 BsaBI GATnn'nnATC Cuts at: 5000 8053 5000 Size: 3053 5006 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2971 3056 5866 6248 Size: 53 83 186 936 1438 85 2810 382 Cuts at: 6248 6883 275 Size: 635 1451 Fragments arranged by size: 2810 1451 1438 936 635 382 186 85 83 53 BsaWI w'CCGG_w Cuts at: 2358 2826 4045 6492 6890 7164 7311 7722 2358 Size: 468 1219 2447 398 274 147 411 2695 Fragments arranged by size: 2695 2447 1219 468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4198 4228 1178 Size: 30 165 30 2795 30 5009 Fragments arranged by size: 5009 2795 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3297 4147 1186 Size: 2111 850 5098 Fragments arranged by size: 5098 2111 850 BseYI C'CCAG_C Cuts at: 3966 7262 7758 3966 Size: 3296 496 4267 Fragments arranged by size: 4267 3296 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4050 1163 Size: 573 2314 5172 Fragments arranged by size: 5172 2314 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5509 6270 7298 1540 Size: 275 186 3508 761 1028 2301 Fragments arranged by size: 3508 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 3922 4975 6120 6205 7276 2118 Size: 1804 1053 1145 85 1071 2901 Fragments arranged by size: 2901 1804 1145 1071 1053 85 BsmI GAATG_Cn' Cuts at: 2156 2628 5570 5663 2156 Size: 472 2942 93 4552 Fragments arranged by size: 4552 2942 472 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2125 3771 3922 4975 5378 6120 Size: 399 328 7 1646 151 1053 403 742 Cuts at: 6120 6205 7276 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1646 1071 1053 742 403 399 328 151 85 7 BspEI T'CCGG_A Cuts at: 2358 2826 7722 2358 Size: 468 4896 2695 Fragments arranged by size: 4896 2695 468 BspHI T'CATG_A Cuts at: 4503 5260 5948 7678 4503 Size: 757 688 1730 4884 Fragments arranged by size: 4884 1730 757 688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2727 3052 3070 5049 5414 844 Size: 1883 325 18 1979 365 3489 Fragments arranged by size: 3489 1979 1883 365 325 18 BsrBI CCG'CTC Cuts at: 1394 1823 4120 5946 1394 Size: 429 2297 1826 3507 Fragments arranged by size: 3507 2297 1826 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2512 2884 4963 6554 6728 65 Size: 1183 1264 372 2079 1591 174 1396 Fragments arranged by size: 2079 1591 1396 1264 1183 372 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6703 6890 1700 Size: 110 4893 187 2869 Fragments arranged by size: 4893 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4889 96 Size: 4793 3266 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8059 BssSI C'ACGA_G Cuts at: 5337 6119 7131 5337 Size: 782 1012 6265 Fragments arranged by size: 6265 1012 782 BstAPI GCAn_nnn'nTGC Cuts at: 794 3410 4730 4965 794 Size: 2616 1320 235 3888 Fragments arranged by size: 3888 2616 1320 235 BstBI TT'CG_AA Cuts at: 6863 6863 Size: 8059 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8059 BstYI r'GATC_y Cuts at: 1972 2130 4263 4651 5389 5756 6141 6158 7610 Size: 158 2133 388 738 367 385 17 1452 Cuts at: 7610 7696 7708 8054 1972 Size: 86 12 346 1977 Fragments arranged by size: 2133 1977 1452 738 388 385 367 346 158 86 17 12 BstZ17I GTA'TAC Cuts at: 2937 2937 Size: 8059 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7507 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7320 Fragments arranged by size: 7320 683 56 BtsI GCAGTG_nn' Cuts at: 2694 4388 5575 6369 6389 2694 Size: 1694 1187 794 20 4364 Fragments arranged by size: 4364 1694 1187 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8059 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8024 DraI TTT'AAA Cuts at: 4555 4717 5363 4555 Size: 162 646 7251 Fragments arranged by size: 7251 646 162 DraIII CAC_nnn'GTG Cuts at: 3926 3926 Size: 8059 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7066 809 Size: 507 5750 1802 Fragments arranged by size: 5750 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5506 6395 8024 Size: 54 1425 50 275 18 3676 889 1629 Cuts at: 8024 8 Size: 43 Fragments arranged by size: 3676 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5506 1537 Size: 275 3694 4090 Fragments arranged by size: 4090 3694 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2425 2744 2976 3255 3420 4185 4190 4670 Size: 1072 319 232 279 165 765 5 480 Cuts at: 4670 5989 1353 Size: 1319 3423 Fragments arranged by size: 3423 1319 1072 765 480 319 279 232 165 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6646 7020 7166 387 Size: 1064 138 5057 374 146 1280 Fragments arranged by size: 5057 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4973 4973 Size: 8059 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2004 2147 2373 2691 3126 3279 3552 Size: 393 372 143 226 318 435 153 273 Cuts at: 3552 3945 4163 5283 6122 6719 7506 7774 1239 Size: 393 218 1120 839 597 787 268 1524 Fragments arranged by size: 1524 1120 839 787 597 435 393 393 372 318 273 268 226 218 153 143 EcoO109I rG'GnC_Cy Cuts at: 2178 2178 Size: 8059 EcoRI G'AATT_C Cuts at: 3840 5368 5746 3840 Size: 1528 378 6153 Fragments arranged by size: 6153 1528 378 EcoRV GAT'ATC Cuts at: 5275 5275 Size: 8059 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2861 4637 2861 Size: 1776 6283 FspI TGC'GCA Cuts at: 2876 6565 2876 Size: 3689 4370 HaeII r_GCGC'y Cuts at: 1678 4150 7206 1678 Size: 2472 3056 2531 Fragments arranged by size: 3056 2531 2472 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3088 3120 3287 3319 6700 Size: 32 135 32 1516 32 167 32 3381 Cuts at: 6700 6732 6774 6806 1373 Size: 32 42 32 2626 Fragments arranged by size: 3381 2626 1516 167 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3756 5403 5649 669 Size: 988 281 1437 381 1647 246 3079 Fragments arranged by size: 3079 1647 1437 988 381 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8059 HpaI GTT'AAC Cuts at: 3375 5649 3375 Size: 2274 5785 KpnI G_GTAC'C Cuts at: 5384 5384 Size: 8059 MfeI C'AATT_G Cuts at: 5658 5658 Size: 8059 MluI A'CGCG_T Cuts at: 7935 7935 Size: 8059 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2684 3386 3755 4109 4241 7173 7357 1305 Size: 1379 702 369 354 132 2932 184 2007 Fragments arranged by size: 2932 2007 1379 702 369 354 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6237 Fragments arranged by size: 6237 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2576 4473 4707 5184 6017 6376 6535 Size: 648 1410 1897 234 477 833 359 159 Cuts at: 6535 518 Size: 2042 Fragments arranged by size: 2042 1897 1410 833 648 477 359 234 159 MspA1I CmG'CkG Cuts at: 1606 2401 5475 6152 7300 7545 1606 Size: 795 3074 677 1148 245 2120 Fragments arranged by size: 3074 2120 1148 795 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7507 NdeI CA'TA_TG Cuts at: 387 4035 387 Size: 3648 4411 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8059 NotI GC'GGCC_GC Cuts at: 5506 5506 Size: 8059 NspI r_CATG'y Cuts at: 1132 1229 4248 4733 4821 5417 6962 1132 Size: 97 3019 485 88 596 1545 2229 Fragments arranged by size: 3019 2229 1545 596 485 97 88 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8059 PciI A'CATG_T Cuts at: 4244 4817 6958 4244 Size: 573 2141 5345 Fragments arranged by size: 5345 2141 573 PflMI CCAn_nnn'nTGG Cuts at: 1108 3836 3992 1108 Size: 2728 156 5175 Fragments arranged by size: 5175 2728 156 PfoI T'CCnGG_A Cuts at: 2686 2686 Size: 8059 PmeI GTTT'AAAC Cuts at: 5363 5363 Size: 8059 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2025 2057 6128 6160 7641 7673 2025 Size: 32 4071 32 1481 32 2411 Fragments arranged by size: 4071 2411 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8059 PsiI TTA'TAA Cuts at: 2214 2606 5629 2214 Size: 392 3023 4644 Fragments arranged by size: 4644 3023 392 PstI C_TGCA'G Cuts at: 830 4726 4961 5411 830 Size: 3896 235 450 3478 Fragments arranged by size: 3896 3478 450 235 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8059 SacI G_AGCT'C Cuts at: 4975 4975 Size: 8059 SalI G'TCGA_C Cuts at: 1936 5401 1936 Size: 3465 4594 SapI GCTCTTCn'nnn_ Cuts at: 3255 3420 3255 Size: 165 7894 SbfI CC_TGCA'GG Cuts at: 5411 5411 Size: 8059 ScaI AGT'ACT Cuts at: 6307 8019 6307 Size: 1712 6347 SfcI C'TryA_G Cuts at: 826 1045 3069 3705 4722 4800 4957 5407 6542 Size: 219 2024 636 1017 78 157 450 1135 Cuts at: 6542 7223 7414 826 Size: 681 191 1471 Fragments arranged by size: 2024 1471 1135 1017 681 636 450 219 191 157 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8059 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8059 SmaI CCC'GGG Cuts at: 1872 5386 1872 Size: 3514 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2369 4267 6163 7064 7326 820 Size: 197 926 426 1898 1896 901 262 1553 Fragments arranged by size: 1898 1896 1553 926 901 426 262 197 SnaBI TAC'GTA Cuts at: 493 5850 493 Size: 5357 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8059 SphI G_CATG'C Cuts at: 1132 5417 1132 Size: 4285 3774 SspI AAT'ATT Cuts at: 5 52 4377 5983 5 Size: 47 4325 1606 2081 Fragments arranged by size: 4325 2081 1606 47 StuI AGG'CCT Cuts at: 1517 4847 1517 Size: 3330 4729 StyI C'CwwG_G Cuts at: 513 1065 5498 513 Size: 552 4433 3074 Fragments arranged by size: 4433 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3196 4033 6086 6103 6256 6441 6886 Size: 1255 1616 837 2053 17 153 185 445 Cuts at: 6886 325 Size: 1498 Fragments arranged by size: 2053 1616 1498 1255 837 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2185 3284 4804 Size: 274 80 33 51 1443 208 1099 1520 Cuts at: 4804 4889 6305 8017 96 Size: 85 1416 1712 138 Fragments arranged by size: 1712 1520 1443 1416 1099 274 208 138 85 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8019 8051 8019 Size: 32 8027 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8059 XbaI T'CTAG_A Cuts at: 5395 5395 Size: 8059 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2270 2504 2011 Size: 259 234 7566 Fragments arranged by size: 7566 259 234 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8059 XmaI C'CCGG_G Cuts at: 1870 5384 1870 Size: 3514 4545 XmnI GAAnn'nnTTC Cuts at: 3030 4620 6188 3030 Size: 1590 1568 4901 Fragments arranged by size: 4901 1590 1568 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5867 276 Size: 53 83 186 936 4333 2468 Fragments arranged by size: 4333 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI