Restriction map for
pFN21AA0042
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BcnI BglI BlnI BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoT22I FseI FspI HapII
HgaI Hin1I HpaI HpaII KasI
KpnI KspI MfeI MroI MspI
MunI NaeI NarI NciI NgoMIV
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccII 1090 CGCG CG^CG
Aor51HI 1823 AGCGCT AGC^GCT
BalI 4622 TGGCCA TGG^CCA
BbvCI 880 CCTCAGC CCTCAGC(-5/-2)
BclI 2541 TGATCA T^GATCA
BlpI 2893 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2893 GCTCAGC GC^T[ACGT]AGC
BspMI 169 GCAGGT ACCTGC(4/8)
BstBI 1306 TTCGAA TT^CGAA
BstEII 4353 GGTTACC G^GT[ACGT]ACC
BstPI 4353 GGTTACC G^GT[ACGT]ACC
BstUI 1090 CGCG CG^CG
CelII 2893 GCTCAGC GC^T[ACGT]AGC
CfoI 1824 GCGC GCG^C
DrdI 3613 GACATACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4622 TGGCCA [CT]^GGCC[GA]
Eco47III 1823 AGCGCT AGC^GCT
EcoO65I 4353 GGTTACC G^GT[ACGT]ACC
FbaI 2541 TGATCA T^GATCA
HaeII 1823 AGCGCT [GA]GCGC^[CT]
HhaI 1824 GCGC GCG^C
HinP1I 1824 GCGC G^CGC
HindIII 3155 AAGCTT A^AGCTT
MluI 1089 ACGCGT A^CGCGT
MluNI 4622 TGGCCA TGG^CCA
MscI 4622 TGGCCA TGG^CCA
MvnI 1090 CGCG CG^CG
NspV 1306 TTCGAA TT^CGAA
PshAI 1260 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 404 CAGCTG CAG^CTG
SfuI 1306 TTCGAA TT^CGAA
SspI 2203 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 28 enzymes) #
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AflIII 349 ACACGT A^C[GA][CT]GT
1089 ACGCGT
AlwNI 380 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2223 CAGGCTCTG
AseI 1876 ATTAAT AT^TAAT
3802 ATTAAT
AsnI 1876 ATTAAT AT^TAAT
3802 ATTAAT
AvaII 1582 GGACC G^G[AT]CC
2758 GGTCC
BsaBI 544 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4579 GATATAAATC
BsiYI 1573 CCTGTTTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2586 CCCAGTTGGGG
BslI 1573 CCTGTTTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2586 CCCAGTTGGGG
BsmFI 2569 GGGAC GGGAC(10/14)
2036 GTCCC
BspHI 1145 TCATGA T^CATGA
3852 TCATGA
BstAPI 2017 GCAATGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2161 GCAGAAATTGC
Cfr13I 1582 GGACC G^G[ACGT]CC
2758 GGTCC
DsaI 4379 CCATGG C^C[GA][CT]GG
4884 CCATGG
EcoRV 733 GATATC GAT^ATC
1630 GATATC
FauI 2038 CCCGC CCCGC(4/6)
4832 GCGGG
HaeIII 2726 GGCC GG^CC
4623 GGCC
HincII 2104 GTCAAC GT[CT]^[GA]AC
3070 GTCAAC
HindII 2104 GTCAAC GT[CT]^[GA]AC
3070 GTCAAC
MamI 544 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4579 GATATAAATC
NcoI 4379 CCATGG C^CATGG
4884 CCATGG
NheI 1252 GCTAGC G^CTAGC
1278 GCTAGC
NlaIV 2757 GGGTCC GG[ACGT]^[ACGT]CC
3519 GGAACC
NspI 230 ACATGC [GA]CATG^[CT]
3931 GCATGT
PshBI 1876 ATTAAT AT^TAAT
3802 ATTAAT
RcaI 1145 TCATGA T^CATGA
3852 TCATGA
Sau96I 1582 GGACC G^G[ACGT]CC
2758 GGTCC
ScaI 13 AGTACT AGT^ACT
638 AGTACT
XbaI 3556 TCTAGA T^CTAGA
4194 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI Asp700I AspHI BanII
BbsI BbvI BfaI BfrI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsmAI BsmI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII PleI PstI RsaI SacI
Sau3AI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI