Restriction map for pFN21AA0042
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# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AocI        
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BcnI        BglI        BlnI        BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstXI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    EcoT22I     FseI        FspI        HapII       
HgaI        Hin1I       HpaI        HpaII       KasI        
KpnI        KspI        MfeI        MroI        MspI        
MunI        NaeI        NarI        NciI        NgoMIV      
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccII        1090  CGCG                 CG^CG
Aor51HI      1823  AGCGCT               AGC^GCT
BalI         4622  TGGCCA               TGG^CCA
BbvCI         880  CCTCAGC              CCTCAGC(-5/-2)
BclI         2541  TGATCA               T^GATCA
BlpI         2893  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2893  GCTCAGC              GC^T[ACGT]AGC
BspMI         169  GCAGGT               ACCTGC(4/8)
BstBI        1306  TTCGAA               TT^CGAA
BstEII       4353  GGTTACC              G^GT[ACGT]ACC
BstPI        4353  GGTTACC              G^GT[ACGT]ACC
BstUI        1090  CGCG                 CG^CG
CelII        2893  GCTCAGC              GC^T[ACGT]AGC
CfoI         1824  GCGC                 GCG^C
DrdI         3613  GACATACAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         4622  TGGCCA               [CT]^GGCC[GA]
Eco47III     1823  AGCGCT               AGC^GCT
EcoO65I      4353  GGTTACC              G^GT[ACGT]ACC
FbaI         2541  TGATCA               T^GATCA
HaeII        1823  AGCGCT               [GA]GCGC^[CT]
HhaI         1824  GCGC                 GCG^C
HinP1I       1824  GCGC                 G^CGC
HindIII      3155  AAGCTT               A^AGCTT
MluI         1089  ACGCGT               A^CGCGT
MluNI        4622  TGGCCA               TGG^CCA
MscI         4622  TGGCCA               TGG^CCA
MvnI         1090  CGCG                 CG^CG
NspV         1306  TTCGAA               TT^CGAA
PshAI        1260  GACAACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         404  CAGCTG               CAG^CTG
SfuI         1306  TTCGAA               TT^CGAA
SspI         2203  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII        349  ACACGT               A^C[GA][CT]GT
             1089  ACGCGT              
AlwNI         380  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2223  CAGGCTCTG           
AseI         1876  ATTAAT               AT^TAAT
             3802  ATTAAT              
AsnI         1876  ATTAAT               AT^TAAT
             3802  ATTAAT              
AvaII        1582  GGACC                G^G[AT]CC
             2758  GGTCC               
BsaBI         544  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4579  GATATAAATC          
BsiYI        1573  CCTGTTTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2586  CCCAGTTGGGG         
BslI         1573  CCTGTTTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2586  CCCAGTTGGGG         
BsmFI        2569  GGGAC                GGGAC(10/14)
             2036  GTCCC               
BspHI        1145  TCATGA               T^CATGA
             3852  TCATGA              
BstAPI       2017  GCAATGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2161  GCAGAAATTGC         
Cfr13I       1582  GGACC                G^G[ACGT]CC
             2758  GGTCC               
DsaI         4379  CCATGG               C^C[GA][CT]GG
             4884  CCATGG              
EcoRV         733  GATATC               GAT^ATC
             1630  GATATC              
FauI         2038  CCCGC                CCCGC(4/6)
             4832  GCGGG               
HaeIII       2726  GGCC                 GG^CC
             4623  GGCC                
HincII       2104  GTCAAC               GT[CT]^[GA]AC
             3070  GTCAAC              
HindII       2104  GTCAAC               GT[CT]^[GA]AC
             3070  GTCAAC              
MamI          544  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4579  GATATAAATC          
NcoI         4379  CCATGG               C^CATGG
             4884  CCATGG              
NheI         1252  GCTAGC               G^CTAGC
             1278  GCTAGC              
NlaIV        2757  GGGTCC               GG[ACGT]^[ACGT]CC
             3519  GGAACC              
NspI          230  ACATGC               [GA]CATG^[CT]
             3931  GCATGT              
PshBI        1876  ATTAAT               AT^TAAT
             3802  ATTAAT              
RcaI         1145  TCATGA               T^CATGA
             3852  TCATGA              
Sau96I       1582  GGACC                G^G[ACGT]CC
             2758  GGTCC               
ScaI           13  AGTACT               AGT^ACT
              638  AGTACT              
XbaI         3556  TCTAGA               T^CTAGA
             4194  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        ApoI        Asp700I     AspHI       BanII       
BbsI        BbvI        BfaI        BfrI        BglII       
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsmAI       BsmI        Bsp1286I    
Bsp1407I    BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       DdeI        
DpnII       EarI        EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeI        NdeII       
NlaIII      PleI        PstI        RsaI        SacI        
Sau3AI      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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