Restriction map for pFN21AA0042
 (Linear) (Six-base) MAPSORT of: KIAA0042.seq  Check: 2084  from: 1  to: 4944



KIAA0042.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     701     931    3527    4300    4629    4772    4944 
   Size:        253     448     230    2596     773     329     143     172

  Fragments arranged by size: 

                2596     773     448     329     253     230     172     143
            
 
AfeI AGC'GCT

Cuts at:      0    1825    4944 
   Size:       1825    3119
 
AflII C'TTAA_G

Cuts at:      0     106     634     886    2250    4944 
   Size:        106     528     252    1364    2694

  Fragments arranged by size: 

                2694    1364     528     252     106
 
AflIII A'CryG_T

Cuts at:      0     349    1089    4944 
   Size:        349     740    3855

  Fragments arranged by size: 

                3855     740     349
 
AleI CACnn'nnGTG

Cuts at:      0    1726    4944 
   Size:       1726    3218
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3197    4944 
   Size:       3197    1747
 
AlwNI CAG_nnn'CTG

Cuts at:      0     385    2228    4944 
   Size:        385    1843    2716

  Fragments arranged by size: 

                2716    1843     385
 
ApoI r'AATT_y

Cuts at:      0     805    1502    1750    2004    2559    2773    3146    3197 
   Size:        805     697     248     254     555     214     373      51

Cuts at:   3197    3593    3636    3693    3719    3818    4234    4504    4944 
   Size:        396      43      57      26      99     416     270     440

  Fragments arranged by size: 

                 805     697     555     440     416     396     373     270
                 254     248     214      99      57      51      43      26
            
 
AseI AT'TA_AT

Cuts at:      0    1877    3803    4944 
   Size:       1877    1926    1141

  Fragments arranged by size: 

                1926    1877    1141
 
BanII G_rGCy'C

Cuts at:      0     895    3259    4883    4909    4944 
   Size:        895    2364    1624      26      35

  Fragments arranged by size: 

                2364    1624     895      35      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     329    1134    3128    4944 
   Size:        329     805    1994    1816

  Fragments arranged by size: 

                1994    1816     805     329
 
BbvCI CC'TCA_GC

Cuts at:      0     881    4944 
   Size:        881    4063
 
BclI T'GATC_A

Cuts at:      0    2541    4944 
   Size:       2541    2403
 
BglII A'GATC_T

Cuts at:      0     163    1416    1806    4393    4944 
   Size:        163    1253     390    2587     551

  Fragments arranged by size: 

                2587    1253     551     390     163
 
BlpI GC'TnA_GC

Cuts at:      0    2894    4944 
   Size:       2894    2050
 
BmgBI CAC'GTC

Cuts at:      0     352    4944 
   Size:        352    4592
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     602    2581    4051    4919    4944 
   Size:        602    1979    1470     868      25

  Fragments arranged by size: 

                1979    1470     868     602      25
 
BmtI G_CTAG'C

Cuts at:      0    1256    1282    4944 
   Size:       1256      26    3662

  Fragments arranged by size: 

                3662    1256      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     914    1845    4944 
   Size:        914     931    3099

  Fragments arranged by size: 

                3099     931     914
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3432    4484    4576    4944 
   Size:       3432    1052      92     368

  Fragments arranged by size: 

                3432    1052     368      92
 
Bpu10I CC'TnA_GC

Cuts at:      0     881    4266    4944 
   Size:        881    3385     678

  Fragments arranged by size: 

                3385     881     678
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     627    1412    2484    4030    4944 
   Size:        627     785    1072    1546     914

  Fragments arranged by size: 

                1546    1072     914     785     627
 
BsaBI GATnn'nnATC

Cuts at:      0     548    4583    4944 
   Size:        548    4035     361

  Fragments arranged by size: 

                4035     548     361
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3558    3588    4944 
   Size:       3558      30    1356

  Fragments arranged by size: 

                3558    1356      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     288    3579    4723    4944 
   Size:        288    3291    1144     221

  Fragments arranged by size: 

                3291    1144     288     221
 
BseYI C'CCAG_C

Cuts at:      0     894    4896    4944 
   Size:        894    4002      48

  Fragments arranged by size: 

                4002     894      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     891    1898    3262    4541    4944 
   Size:        891    1007    1364    1279     403

  Fragments arranged by size: 

                1364    1279    1007     891     403
 
BsiHKAI G_wGCw'C

Cuts at:      0    3259    4500    4883    4909    4944 
   Size:       3259    1241     383      26      35

  Fragments arranged by size: 

                3259    1241     383      35      26
 
BsmI GAATG_Cn'

Cuts at:      0      87    3642    4122    4921    4944 
   Size:         87    3555     480     799      23

  Fragments arranged by size: 

                3555     799     480      87      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     895    3259    4500    4883    4909    4944 
   Size:        895    2364    1241     383      26      35

  Fragments arranged by size: 

                2364    1241     895     383      35      26
 
BspHI T'CATG_A

Cuts at:      0    1145    3852    4944 
   Size:       1145    2707    1092

  Fragments arranged by size: 

                2707    1145    1092
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     160    4944 
   Size:        160    4784
 
BsrDI GCAATG_nn'

Cuts at:      0     659    2024    2108    3452    4521    4944 
   Size:        659    1365      84    1344    1069     423

  Fragments arranged by size: 

                1365    1344    1069     659     423      84
 
BsrGI T'GTAC_A

Cuts at:      0     841    4131    4339    4944 
   Size:        841    3290     208     605

  Fragments arranged by size: 

                3290     841     605     208
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2023    2167    4944 
   Size:       2023     144    2777

  Fragments arranged by size: 

                2777    2023     144
 
BstBI TT'CG_AA

Cuts at:      0    1307    4944 
   Size:       1307    3637
 
BstEII G'GTnAC_C

Cuts at:      0    4353    4944 
   Size:       4353     591
 
BstYI r'GATC_y

Cuts at:      0     163     549     982    1416    1806    2424    4393    4944 
   Size:        163     386     433     434     390     618    1969     551

  Fragments arranged by size: 

                1969     618     551     434     433     390     386     163
            
 
BtgI C'CryG_G

Cuts at:      0    4379    4884    4944 
   Size:       4379     505      60

  Fragments arranged by size: 

                4379     505      60
 
BtsI GCAGTG_nn'

Cuts at:      0    1824    4342    4907    4944 
   Size:       1824    2518     565      37

  Fragments arranged by size: 

                2518    1824     565      37
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2284    2319    4944 
   Size:       2284      35    2625

  Fragments arranged by size: 

                2625    2284      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3619    4944 
   Size:       3619    1325
 
EaeI y'GGCC_r

Cuts at:      0    4622    4944 
   Size:       4622     322
 
EarI CTCTTCn'nnn_

Cuts at:      0     534    2730    2960    4944 
   Size:        534    2196     230    1984

  Fragments arranged by size: 

                2196    1984     534     230
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3124    4944 
   Size:       3124    1820
 
EcoICRI GAG'CTC

Cuts at:      0    3257    4881    4907    4944 
   Size:       3257    1624      26      37

  Fragments arranged by size: 

                3257    1624      37      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     701     931    3432    3527    4300    4484    4576 
   Size:        253     448     230    2501      95     773     184      92

Cuts at:   4576    4629    4772    4944 
   Size:         53     143     172

  Fragments arranged by size: 

                2501     773     448     253     230     184     172     143
                  95      92      53
 
EcoRI G'AATT_C

Cuts at:      0    3146    4234    4504    4944 
   Size:       3146    1088     270     440

  Fragments arranged by size: 

                3146    1088     440     270
 
EcoRV GAT'ATC

Cuts at:      0     735    1632    4944 
   Size:        735     897    3312

  Fragments arranged by size: 

                3312     897     735
 
HaeII r_GCGC'y

Cuts at:      0    1827    4944 
   Size:       1827    3117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1554    1586    2181    2210    2213    2242    4075    4107 
   Size:       1554      32     595      29       3      29    1833      32

Cuts at:   4107    4380    4412    4476    4508    4944 
   Size:        273      32      64      32     436

  Fragments arranged by size: 

                1833    1554     595     436     273      64      32      32
                  32      32      29      29       3
 
HincII GTy'rAC

Cuts at:      0    2106    3072    4944 
   Size:       2106     966    1872

  Fragments arranged by size: 

                2106    1872     966
 
HindIII A'AGCT_T

Cuts at:      0    3155    4944 
   Size:       3155    1789
 
MluI A'CGCG_T

Cuts at:      0    1089    4944 
   Size:       1089    3855
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     299     710    2179    2460    4944 
   Size:        104     195     411    1469     281    2484

  Fragments arranged by size: 

                2484    1469     411     281     195     104
 
MscI TGG'CCA

Cuts at:      0    4624    4944 
   Size:       4624     320
 
MslI CAynn'nnrTG

Cuts at:      0     565    1471    1726    1844    4944 
   Size:        565     906     255     118    3100

  Fragments arranged by size: 

                3100     906     565     255     118
 
MspA1I CmG'CkG

Cuts at:      0      35     406    2041    4944 
   Size:         35     371    1635    2903

  Fragments arranged by size: 

                2903    1635     371      35
 
NcoI C'CATG_G

Cuts at:      0    4379    4884    4944 
   Size:       4379     505      60

  Fragments arranged by size: 

                4379     505      60
 
NdeI CA'TA_TG

Cuts at:      0    1587    2607    2925    4944 
   Size:       1587    1020     318    2019

  Fragments arranged by size: 

                2019    1587    1020     318
 
NheI G'CTAG_C

Cuts at:      0    1252    1278    4944 
   Size:       1252      26    3666

  Fragments arranged by size: 

                3666    1252      26
 
NspI r_CATG'y

Cuts at:      0     234    3935    4944 
   Size:        234    3701    1009

  Fragments arranged by size: 

                3701    1009     234
 
PasI CC'CwG_GG

Cuts at:      0    4778    4944 
   Size:       4778     166
 
PfoI T'CCnGG_A

Cuts at:      0    3714    4226    4944 
   Size:       3714     512     718

  Fragments arranged by size: 

                3714     718     512
 
PshAI GACnn'nnGTC

Cuts at:      0    1264    4944 
   Size:       1264    3680
 
PsiI TTA'TAA

Cuts at:      0    1196    3824    4790    4944 
   Size:       1196    2628     966     154

  Fragments arranged by size: 

                2628    1196     966     154
 
PstI C_TGCA'G

Cuts at:      0     171     390    1041    4944 
   Size:        171     219     651    3903

  Fragments arranged by size: 

                3903     651     219     171
 
PvuII CAG'CTG

Cuts at:      0     406    4944 
   Size:        406    4538
 
SacI G_AGCT'C

Cuts at:      0    3259    4883    4909    4944 
   Size:       3259    1624      26      35

  Fragments arranged by size: 

                3259    1624      35      26
 
ScaI AGT'ACT

Cuts at:      0      15     640    4944 
   Size:         15     625    4304

  Fragments arranged by size: 

                4304     625      15
 
SfcI C'TryA_G

Cuts at:      0     167     251     386    1019    1037    2474    3202    3785 
   Size:        167      84     135     633      18    1437     728     583

Cuts at:   3785    4944 
   Size:       1159

  Fragments arranged by size: 

                1437    1159     728     633     583     167     135      84
                  18
 
SmlI C'TyrA_G

Cuts at:      0     106     634     642     886    1427    2250    2499    4009 
   Size:        106     528       8     244     541     823     249    1510

Cuts at:   4009    4944 
   Size:        935

  Fragments arranged by size: 

                1510     935     823     541     528     249     244     106
                   8
 
SspI AAT'ATT

Cuts at:      0    2205    4944 
   Size:       2205    2739
 
StyI C'CwwG_G

Cuts at:      0    3845    4379    4884    4944 
   Size:       3845     534     505      60

  Fragments arranged by size: 

                3845     534     505      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     106     672    1987    4929    4938    4944 
   Size:        106     566    1315    2942       9       6

  Fragments arranged by size: 

                2942    1315     566     106       9       6
 
TatI w'GTAC_w

Cuts at:      0      13     638     841    2548    3192    4131    4339    4446 
   Size:         13     625     203    1707     644     939     208     107

Cuts at:   4446    4944 
   Size:        498

  Fragments arranged by size: 

                1707     939     644     625     498     208     203     107
                  13
 
XbaI T'CTAG_A

Cuts at:      0    3556    4194    4944 
   Size:       3556     638     750

  Fragments arranged by size: 

                3556     750     638
 
XmnI GAAnn'nnTTC

Cuts at:      0    1502    1967    3594    4610    4944 
   Size:       1502     465    1627    1016     334

  Fragments arranged by size: 

                1627    1502    1016     465     334
 

 Enzymes that do cut:

     AcuI     AfeI    AflII   AflIII     AleI     AlfI    AlwNI     ApoI
     AseI    BanII     BbsI    BbvCI     BclI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstYI     BtgI     BtsI
    CspCI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoRI
    EcoRV    HaeII    Hin4I   HincII  HindIII     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NspI     PasI     PfoI
    PshAI     PsiI     PstI    PvuII     SacI     ScaI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BcgI    BciVI    BfrBI     BglI Bme1580I     BsaI
    BsaAI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
    BsrFI   BssHII    BssSI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     EagI    EcoNI EcoO109I     FalI     FseI     FspI
    FspAI     HpaI     KasI     KpnI     MfeI     NaeI     NarI   NgoMIV
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com