(Circular) (Six-base) MAPSORT of: pFN21AA0010.seq Check: 1844 from: 1 to: 7954 pFN21AA0010 7954 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5162 5162 Size: 7954 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5764 278 Size: 53 83 186 936 4228 2468 Fragments arranged by size: 4228 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5297 1937 Size: 3360 4594 Acc65I G'GTAC_C Cuts at: 3322 3430 5275 3322 Size: 108 1845 6001 Fragments arranged by size: 6001 1845 108 AclI AA'CG_TT Cuts at: 1563 2341 6081 6454 1563 Size: 778 3740 373 3063 Fragments arranged by size: 3740 3063 778 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3212 3425 4367 4643 5060 5189 6017 7401 Size: 1580 213 942 276 417 129 828 1384 Cuts at: 7401 7669 1632 Size: 268 1917 Fragments arranged by size: 1917 1580 1384 942 828 417 276 268 213 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7757 AflIII A'CryG_T Cuts at: 1203 3949 6853 7830 1203 Size: 2746 2904 977 1327 Fragments arranged by size: 2904 2746 1327 977 AgeI A'CCGG_T Cuts at: 6785 6785 Size: 7954 AhdI GACnn_n'nnGTC Cuts at: 6683 6683 Size: 7954 AleI CACnn'nnGTG Cuts at: 1166 4670 1166 Size: 3504 4450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 5180 1184 Size: 1299 2697 3958 Fragments arranged by size: 3958 2697 1299 AlwNI CAG_nnn'CTG Cuts at: 2308 5154 5697 7269 2308 Size: 2846 543 1572 2993 Fragments arranged by size: 2993 2846 1572 543 ApaI G_GGCC'C Cuts at: 3001 3001 Size: 7954 ApaLI G'TGCA_C Cuts at: 4265 4902 6011 7167 4265 Size: 637 1109 1156 5052 Fragments arranged by size: 5052 1156 1109 637 ApoI r'AATT_y Cuts at: 1493 2595 2709 3557 3862 4609 4712 4881 5263 Size: 1102 114 848 305 747 103 169 382 Cuts at: 5263 5489 5641 7750 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1102 848 747 382 305 226 169 152 114 103 AseI AT'TA_AT Cuts at: 160 1032 4173 4892 6508 160 Size: 872 3141 719 1616 1606 Fragments arranged by size: 3141 1616 1606 872 719 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7954 AvaI C'yCGr_G Cuts at: 1870 1943 2691 4850 5177 5279 1870 Size: 73 748 2159 327 102 4545 Fragments arranged by size: 4545 2159 748 327 102 73 BamHI G'GATC_C Cuts at: 4257 5284 5651 4257 Size: 1027 367 6560 Fragments arranged by size: 6560 1027 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3322 3430 5275 6730 618 Size: 325 217 627 1535 108 1845 1455 1842 Fragments arranged by size: 1845 1842 1535 1455 627 325 217 108 BanII G_rGCy'C Cuts at: 1391 2892 3001 5273 1391 Size: 1501 109 2272 4072 Fragments arranged by size: 4072 2272 1501 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2337 3259 4641 4981 928 Size: 971 438 922 1382 340 3901 Fragments arranged by size: 3901 1382 971 922 438 340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3642 3676 6145 6179 1903 Size: 34 1705 34 2469 34 3678 Fragments arranged by size: 3678 2469 1705 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5103 5846 7056 5103 Size: 743 1210 6001 Fragments arranged by size: 6001 1210 743 BclI T'GATC_A Cuts at: 1548 3414 4870 1548 Size: 1866 1456 4632 Fragments arranged by size: 4632 1866 1456 BfrBI ATG'CAT Cuts at: 3659 3774 3659 Size: 115 7839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6565 136 Size: 107 122 71 631 605 4893 1525 Fragments arranged by size: 4893 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4104 7949 4104 Size: 3845 4109 BlpI GC'TnA_GC Cuts at: 5249 5371 5249 Size: 122 7832 Bme1580I G_kGCm'C Cuts at: 1790 3001 4269 4906 6015 7171 1790 Size: 1211 1268 637 1109 1156 2573 Fragments arranged by size: 2573 1268 1211 1156 1109 637 BmgBI CAC'GTC Cuts at: 1318 4006 4564 1318 Size: 2688 558 4708 Fragments arranged by size: 4708 2688 558 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5685 6643 442 Size: 416 53 83 399 24 4268 958 1753 Fragments arranged by size: 4268 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2571 1056 Size: 1515 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4212 5014 6614 1239 Size: 2973 802 1600 2579 Fragments arranged by size: 2973 2579 1600 802 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3124 5150 6079 6944 7242 3124 Size: 2026 929 865 298 3836 Fragments arranged by size: 3836 2026 929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2637 6617 882 Size: 617 1138 3980 2219 Fragments arranged by size: 3980 2219 1138 617 BsaAI yAC'GTr Cuts at: 493 1204 5745 493 Size: 711 4541 2702 Fragments arranged by size: 4541 2702 711 BsaBI GATnn'nnATC Cuts at: 7948 7948 Size: 7954 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5761 6143 6778 275 Size: 53 83 186 936 4228 382 635 1451 Fragments arranged by size: 4228 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2761 4749 6387 6785 7059 7206 7617 2761 Size: 1988 1638 398 274 147 411 3098 Fragments arranged by size: 3098 1988 1638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4568 4598 5105 5135 1178 Size: 30 165 30 3165 30 507 30 3997 Fragments arranged by size: 3997 3165 507 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2816 3125 3128 3275 3871 4661 1186 Size: 1630 309 3 147 596 790 4479 Fragments arranged by size: 4479 1630 790 596 309 147 3 BseYI C'CCAG_C Cuts at: 7157 7653 7157 Size: 496 7458 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3326 1163 Size: 573 1590 5791 Fragments arranged by size: 5791 1590 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5404 6165 7193 1540 Size: 275 186 3403 761 1028 2301 Fragments arranged by size: 3403 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2950 4269 4906 6015 6100 7171 2950 Size: 1319 637 1109 85 1071 3733 Fragments arranged by size: 3733 1319 1109 1071 637 85 BsmI GAATG_Cn' Cuts at: 2520 2905 3191 4479 5465 5558 2520 Size: 385 286 1288 986 93 4916 Fragments arranged by size: 4916 1288 986 385 286 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2892 2950 3001 4269 4906 5273 6015 Size: 399 1102 58 51 1268 637 367 742 Cuts at: 6015 6100 7171 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1268 1102 1071 742 637 399 367 85 58 51 BspEI T'CCGG_A Cuts at: 2761 7617 2761 Size: 4856 3098 BspHI T'CATG_A Cuts at: 5843 7573 5843 Size: 1730 6224 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2451 4687 5162 5172 5309 844 Size: 1607 2236 475 10 137 3489 Fragments arranged by size: 3489 2236 1607 475 137 10 BsrBI CCG'CTC Cuts at: 1394 1823 5134 5841 1394 Size: 429 3311 707 3507 Fragments arranged by size: 3507 3311 707 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2265 2735 6449 6623 65 Size: 1183 1017 470 3714 174 1396 Fragments arranged by size: 3714 1396 1183 1017 470 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6598 6785 1700 Size: 110 4788 187 2869 Fragments arranged by size: 4788 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7954 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7954 BssSI C'ACGA_G Cuts at: 3853 6014 7026 3853 Size: 2161 1012 4781 Fragments arranged by size: 4781 2161 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7954 BstBI TT'CG_AA Cuts at: 2016 6758 2016 Size: 4742 3212 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7954 BstXI CCAn_nnnn'nTGG Cuts at: 3882 3882 Size: 7954 BstYI r'GATC_y Cuts at: 1972 2405 4104 4257 4797 5284 5651 6036 6053 Size: 433 1699 153 540 487 367 385 17 Cuts at: 6053 7505 7591 7603 7949 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1699 1452 540 487 433 385 367 346 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4596 4596 Size: 7954 BtgI C'CryG_G Cuts at: 513 1065 3276 4282 513 Size: 552 2211 1006 4185 Fragments arranged by size: 4185 2211 1006 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7215 Fragments arranged by size: 7215 683 56 BtsI GCAGTG_nn' Cuts at: 4807 5470 6264 6284 4807 Size: 663 794 20 6477 Fragments arranged by size: 6477 794 663 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7954 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7919 DraI TTT'AAA Cuts at: 4335 5258 4335 Size: 923 7031 DraIII CAC_nnn'GTG Cuts at: 4771 4771 Size: 7954 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4733 6961 809 Size: 507 3417 2228 1802 Fragments arranged by size: 3417 2228 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5401 6290 7919 Size: 54 1425 50 275 18 3571 889 1629 Cuts at: 7919 8 Size: 43 Fragments arranged by size: 3571 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5401 1537 Size: 275 3589 4090 Fragments arranged by size: 4090 3589 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3618 4619 5884 1353 Size: 2265 1001 1265 3423 Fragments arranged by size: 3423 2265 1265 1001 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3982 4790 4811 6541 6915 7061 Size: 1064 138 2393 808 21 1730 374 146 Cuts at: 7061 387 Size: 1280 Fragments arranged by size: 2393 1730 1280 1064 808 374 146 138 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3212 3425 4212 4367 4643 5014 5060 Size: 393 1580 213 787 155 276 371 46 Cuts at: 5060 5189 6017 6614 7401 7669 1239 Size: 129 828 597 787 268 1524 Fragments arranged by size: 1580 1524 828 787 787 597 393 371 276 268 213 155 129 46 EcoNI CCTnn'n_nnAGG Cuts at: 3005 4688 3005 Size: 1683 6271 EcoO109I rG'GnC_Cy Cuts at: 2997 2998 4828 4927 2997 Size: 1 1830 99 6024 Fragments arranged by size: 6024 1830 99 1 EcoRI G'AATT_C Cuts at: 3557 5263 5641 3557 Size: 1706 378 5870 Fragments arranged by size: 5870 1706 378 EcoRV GAT'ATC Cuts at: 3450 3450 Size: 7954 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3353 3353 Size: 7954 FspI TGC'GCA Cuts at: 2868 6460 2868 Size: 3592 4362 HaeII r_GCGC'y Cuts at: 1678 2259 2858 4746 7101 1678 Size: 581 599 1888 2355 2531 Fragments arranged by size: 2531 2355 1888 599 581 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3122 3154 4089 4121 4919 Size: 32 135 32 1550 32 935 32 798 Cuts at: 4919 4951 5055 5087 6595 6627 6669 6701 1373 Size: 32 104 32 1508 32 42 32 2626 Fragments arranged by size: 2626 1550 1508 935 798 135 104 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 5298 5544 669 Size: 988 281 1467 1893 246 3079 Fragments arranged by size: 3079 1893 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 2657 748 Size: 1909 6045 HpaI GTT'AAC Cuts at: 5544 5544 Size: 7954 KpnI G_GTAC'C Cuts at: 3326 3434 5279 3326 Size: 108 1845 6001 Fragments arranged by size: 6001 1845 108 MfeI C'AATT_G Cuts at: 5553 5553 Size: 7954 MluI A'CGCG_T Cuts at: 7830 7830 Size: 7954 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 5148 7068 7252 1305 Size: 1074 2769 1920 184 2007 Fragments arranged by size: 2769 2007 1920 1074 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6132 Fragments arranged by size: 6132 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3275 3299 3779 4505 4670 5156 5912 Size: 648 2109 24 480 726 165 486 756 Cuts at: 5912 6271 6430 518 Size: 359 159 2042 Fragments arranged by size: 2109 2042 756 726 648 486 480 359 165 159 24 MspA1I CmG'CkG Cuts at: 1606 3988 4027 5237 5370 6047 7195 7440 1606 Size: 2382 39 1210 133 677 1148 245 2120 Fragments arranged by size: 2382 2120 1210 1148 677 245 133 39 NcoI C'CATG_G Cuts at: 513 1065 3276 4282 513 Size: 552 2211 1006 4185 Fragments arranged by size: 4185 2211 1006 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7954 NheI G'CTAG_C Cuts at: 1052 2567 1052 Size: 1515 6439 NotI GC'GGCC_GC Cuts at: 5401 5401 Size: 7954 NsiI A_TGCA'T Cuts at: 3661 3776 3661 Size: 115 7839 NspI r_CATG'y Cuts at: 1132 1229 2424 3663 3953 4504 5312 6857 1132 Size: 97 1195 1239 290 551 808 1545 2229 Fragments arranged by size: 2229 1545 1239 1195 808 551 290 97 PacI TTA_AT'TAA Cuts at: 2370 2370 Size: 7954 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7954 PciI A'CATG_T Cuts at: 3949 6853 3949 Size: 2904 5050 PflMI CCAn_nnn'nTGG Cuts at: 1108 4288 4771 1108 Size: 3180 483 4291 Fragments arranged by size: 4291 3180 483 PmeI GTTT'AAAC Cuts at: 5258 5258 Size: 7954 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2911 2943 6023 6055 7536 7568 2911 Size: 32 3080 32 1481 32 3297 Fragments arranged by size: 3297 3080 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4927 4927 Size: 7954 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7954 PsiI TTA'TAA Cuts at: 5524 5524 Size: 7954 PspOMI G'GGCC_C Cuts at: 2997 2997 Size: 7954 PspXI vC'TCGA_Gb Cuts at: 4850 4850 Size: 7954 PstI C_TGCA'G Cuts at: 830 3042 3993 4969 5306 830 Size: 2212 951 976 337 3478 Fragments arranged by size: 3478 2212 976 951 337 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7954 SalI G'TCGA_C Cuts at: 1936 5296 1936 Size: 3360 4594 SapI GCTCTTCn'nnn_ Cuts at: 3618 4619 3618 Size: 1001 6953 SbfI CC_TGCA'GG Cuts at: 5306 5306 Size: 7954 ScaI AGT'ACT Cuts at: 6202 7914 6202 Size: 1712 6242 SexAI A'CCwGG_T Cuts at: 3234 3234 Size: 7954 SfcI C'TryA_G Cuts at: 826 1045 2179 2700 3038 3343 3989 4780 4965 Size: 219 1134 521 338 305 646 791 185 Cuts at: 4965 5302 6437 7118 7309 826 Size: 337 1135 681 191 1471 Fragments arranged by size: 1471 1135 1134 791 681 646 521 338 337 305 219 191 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7954 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7954 SmaI CCC'GGG Cuts at: 1872 5281 1872 Size: 3409 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2691 3139 4850 5129 6058 6959 Size: 197 926 748 448 1711 279 929 901 Cuts at: 6959 7221 820 Size: 262 1553 Fragments arranged by size: 1711 1553 929 926 901 748 448 279 262 197 SnaBI TAC'GTA Cuts at: 493 5745 493 Size: 5252 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7954 SphI G_CATG'C Cuts at: 1132 3663 5312 1132 Size: 2531 1649 3774 Fragments arranged by size: 3774 2531 1649 SspI AAT'ATT Cuts at: 5 52 2201 2678 5878 5 Size: 47 2149 477 3200 2081 Fragments arranged by size: 3200 2149 2081 477 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7954 StyI C'CwwG_G Cuts at: 513 1065 2040 2050 3276 4282 5393 513 Size: 552 975 10 1226 1006 1111 3074 Fragments arranged by size: 3074 1226 1111 1006 975 552 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5981 5998 6151 6336 6781 325 Size: 1255 4401 17 153 185 445 1498 Fragments arranged by size: 4401 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6200 7912 96 Size: 274 80 33 51 1443 4223 1712 138 Fragments arranged by size: 4223 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4568 4600 7914 7946 4568 Size: 32 3314 32 4576 Fragments arranged by size: 4576 3314 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7954 XbaI T'CTAG_A Cuts at: 5290 5290 Size: 7954 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4003 4561 4003 Size: 558 7396 XhoI C'TCGA_G Cuts at: 1943 2691 4850 1943 Size: 748 2159 5047 Fragments arranged by size: 5047 2159 748 XmaI C'CCGG_G Cuts at: 1870 5279 1870 Size: 3409 4545 XmnI GAAnn'nnTTC Cuts at: 4398 6083 4398 Size: 1685 6269 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5762 276 Size: 53 83 186 936 4228 2468 Fragments arranged by size: 4228 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI