(Circular) (Six-base) MAPSORT of: pFN21ASDF0400.seq Check: 4945 from: 1 to: 6907 pFN21ASDF0400 6907 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4717 278 Size: 53 83 186 936 3181 2468 Fragments arranged by size: 3181 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4250 1937 Size: 2313 4594 Acc65I G'GTAC_C Cuts at: 4228 4228 Size: 6907 AclI AA'CG_TT Cuts at: 1563 3258 5034 5407 1563 Size: 1695 1776 373 3063 Fragments arranged by size: 3063 1776 1695 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3182 3587 3802 4970 6354 6622 1632 Size: 1550 405 215 1168 1384 268 1917 Fragments arranged by size: 1917 1550 1384 1168 405 268 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6710 AflIII A'CryG_T Cuts at: 1203 5806 6783 1203 Size: 4603 977 1327 Fragments arranged by size: 4603 1327 977 AgeI A'CCGG_T Cuts at: 3384 5738 3384 Size: 2354 4553 AhdI GACnn_n'nnGTC Cuts at: 5636 5636 Size: 6907 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2804 1184 Size: 1620 5287 AlwNI CAG_nnn'CTG Cuts at: 2272 3037 4650 6222 2272 Size: 765 1613 1572 2957 Fragments arranged by size: 2957 1613 1572 765 ApaI G_GGCC'C Cuts at: 2059 2893 4004 4100 2059 Size: 834 1111 96 4866 Fragments arranged by size: 4866 1111 834 96 ApaLI G'TGCA_C Cuts at: 4964 6120 4964 Size: 1156 5751 ApoI r'AATT_y Cuts at: 1493 2337 4216 4442 4594 6703 1493 Size: 844 1879 226 152 2109 1697 Fragments arranged by size: 2109 1879 1697 844 226 152 AseI AT'TA_AT Cuts at: 160 1032 5461 160 Size: 872 4429 1606 Fragments arranged by size: 4429 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6907 AvaI C'yCGr_G Cuts at: 1870 1943 2277 2886 3985 4072 4232 1870 Size: 73 334 609 1099 87 160 4545 Fragments arranged by size: 4545 1099 609 334 160 87 73 BamHI G'GATC_C Cuts at: 4237 4604 4237 Size: 367 6540 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2247 2740 3942 4151 Size: 325 217 627 278 182 493 1202 209 Cuts at: 4151 4228 5683 618 Size: 77 1455 1842 Fragments arranged by size: 1842 1455 1202 627 493 325 278 217 209 182 77 BanII G_rGCy'C Cuts at: 1391 2059 2187 2292 2893 2946 3771 3809 4004 Size: 668 128 105 601 53 825 38 195 Cuts at: 4004 4100 4226 1391 Size: 96 126 4072 Fragments arranged by size: 4072 825 668 601 195 128 126 105 96 53 38 BbeI G_GCGC'C Cuts at: 2251 2744 2251 Size: 493 6414 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2720 2752 3517 928 Size: 971 821 32 765 4318 Fragments arranged by size: 4318 971 821 765 32 BbvCI CC'TCA_GC Cuts at: 2642 2642 Size: 6907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5098 5132 1903 Size: 34 3161 34 3678 Fragments arranged by size: 3678 3161 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4799 6009 4799 Size: 1210 5697 BclI T'GATC_A Cuts at: 1548 1548 Size: 6907 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2328 2897 3370 Size: 107 122 71 631 605 656 569 473 Cuts at: 3370 5518 136 Size: 2148 1525 Fragments arranged by size: 2148 1525 656 631 605 569 473 122 107 71 BglII A'GATC_T Cuts at: 6902 6902 Size: 6907 BlpI GC'TnA_GC Cuts at: 2387 3842 4324 2387 Size: 1455 482 4970 Fragments arranged by size: 4970 1455 482 Bme1580I G_kGCm'C Cuts at: 1790 2059 2483 2893 3666 3793 4004 4100 4156 Size: 269 424 410 773 127 211 96 56 Cuts at: 4156 4968 6124 1790 Size: 812 1156 2573 Fragments arranged by size: 2573 1156 812 773 424 410 269 211 127 96 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6907 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4638 5596 442 Size: 416 53 83 399 24 3221 958 1753 Fragments arranged by size: 3221 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6907 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2626 4011 2626 Size: 1385 5522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 2867 3014 3125 3559 3589 5567 1239 Size: 1220 408 147 111 434 30 1978 2579 Fragments arranged by size: 2579 1978 1220 434 408 147 111 30 Bpu10I CC'TnA_GC Cuts at: 2642 2642 Size: 6907 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5032 5897 6195 5032 Size: 865 298 5744 Fragments arranged by size: 5744 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5570 882 Size: 617 4071 2219 Fragments arranged by size: 4071 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4204 4698 493 Size: 711 3000 494 2702 Fragments arranged by size: 3000 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6901 6901 Size: 6907 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2123 2248 2741 4714 Size: 53 83 186 936 590 125 493 1973 Cuts at: 4714 5096 5731 275 Size: 382 635 1451 Fragments arranged by size: 1973 1451 936 635 590 493 382 186 125 83 53 BsaWI w'CCGG_w Cuts at: 3384 3906 5340 5738 6012 6159 6570 3384 Size: 522 1434 398 274 147 411 3721 Fragments arranged by size: 3721 1434 522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2099 3002 3032 3287 Size: 30 165 30 666 30 903 30 255 Cuts at: 3287 3317 3791 3821 3944 3974 4001 4031 1178 Size: 30 474 30 123 30 27 30 4054 Fragments arranged by size: 4054 903 666 474 255 165 123 30 30 30 30 30 30 30 30 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2630 2654 2755 2864 3023 3311 3320 Size: 907 537 24 101 109 159 288 9 Cuts at: 3320 3437 3626 3685 3821 1186 Size: 117 189 59 136 4272 Fragments arranged by size: 4272 907 537 288 189 159 136 117 109 101 59 24 9 BseYI C'CCAG_C Cuts at: 2722 3276 4184 6110 6606 2722 Size: 554 908 1926 496 3023 Fragments arranged by size: 3023 1926 908 554 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 2810 1163 Size: 573 480 594 5260 Fragments arranged by size: 5260 594 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4094 4357 5118 6146 1540 Size: 275 186 2093 263 761 1028 2301 Fragments arranged by size: 2301 2093 1028 761 275 263 186 BsiHKAI G_wGCw'C Cuts at: 3636 4968 5053 6124 3636 Size: 1332 85 1071 4419 Fragments arranged by size: 4419 1332 1071 85 BsmI GAATG_Cn' Cuts at: 4418 4511 4418 Size: 93 6814 BsmBI CGTCTCn'nnnn_ Cuts at: 3196 3871 3196 Size: 675 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2187 2292 2483 2893 2946 3636 Size: 399 269 128 105 191 410 53 690 Cuts at: 3636 3666 3771 3793 3809 4004 4100 4156 4226 Size: 30 105 22 16 195 96 56 70 Cuts at: 4226 4968 5053 6124 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 690 410 399 269 195 191 128 105 105 96 85 70 56 53 30 22 16 BspEI T'CCGG_A Cuts at: 3906 6570 3906 Size: 2664 4243 BspHI T'CATG_A Cuts at: 4796 6526 4796 Size: 1730 5177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2928 3045 3916 4262 844 Size: 1547 537 117 871 346 3489 Fragments arranged by size: 3489 1547 871 537 346 117 BsrBI CCG'CTC Cuts at: 1394 1823 2053 2104 4794 1394 Size: 429 230 51 2690 3507 Fragments arranged by size: 3507 2690 429 230 51 BsrDI GCAATG_nn' Cuts at: 65 1248 5402 5576 65 Size: 1183 4154 174 1396 Fragments arranged by size: 4154 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2226 3384 3396 5551 5738 1700 Size: 110 416 1158 12 2155 187 2869 Fragments arranged by size: 2869 2155 1158 416 187 110 12 BsrGI T'GTAC_A Cuts at: 96 2616 3303 96 Size: 2520 687 3700 Fragments arranged by size: 3700 2520 687 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6907 BssSI C'ACGA_G Cuts at: 4967 5979 4967 Size: 1012 5895 BstAPI GCAn_nnn'nTGC Cuts at: 794 2203 794 Size: 1409 5498 BstBI TT'CG_AA Cuts at: 2007 5711 2007 Size: 3704 3203 BstEII G'GTnAC_C Cuts at: 1184 3355 3472 1184 Size: 2171 117 4619 Fragments arranged by size: 4619 2171 117 BstXI CCAn_nnnn'nTGG Cuts at: 2483 2483 Size: 6907 BstYI r'GATC_y Cuts at: 1972 4237 4604 4989 5006 6458 6544 6556 6902 Size: 2265 367 385 17 1452 86 12 346 Cuts at: 6902 1972 Size: 1977 Fragments arranged by size: 2265 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2033 2187 3288 3336 4088 4116 513 Size: 552 968 154 1101 48 752 28 3304 Fragments arranged by size: 3304 1101 968 752 552 154 48 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3037 487 Size: 683 56 1811 4357 Fragments arranged by size: 4357 1811 683 56 BtsI GCAGTG_nn' Cuts at: 3666 4423 5217 5237 3666 Size: 757 794 20 5336 Fragments arranged by size: 5336 794 757 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6907 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6872 DraI TTT'AAA Cuts at: 4211 4211 Size: 6907 DraIII CAC_nnn'GTG Cuts at: 3949 3949 Size: 6907 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5914 809 Size: 507 4598 1802 Fragments arranged by size: 4598 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3168 3781 4091 Size: 54 1425 50 275 18 1338 613 310 Cuts at: 4091 4354 5243 6872 8 Size: 263 889 1629 43 Fragments arranged by size: 1629 1425 1338 889 613 310 275 263 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4091 4354 1537 Size: 275 2279 263 4090 Fragments arranged by size: 4090 2279 275 263 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2579 3494 3536 3719 4837 1353 Size: 977 249 915 42 183 1118 3423 Fragments arranged by size: 3423 1118 977 915 249 183 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2426 2615 2704 4153 5494 5868 Size: 1064 138 837 189 89 1449 1341 374 Cuts at: 5868 6014 387 Size: 146 1280 Fragments arranged by size: 1449 1341 1280 1064 837 374 189 146 138 89 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2459 2867 3014 3125 3182 3559 3587 Size: 393 827 408 147 111 57 377 28 Cuts at: 3587 3589 3802 4970 5567 6354 6622 1239 Size: 2 213 1168 597 787 268 1524 Fragments arranged by size: 1524 1168 827 787 597 408 393 377 268 213 147 111 57 28 2 EcoNI CCTnn'n_nnAGG Cuts at: 2255 2448 3950 2255 Size: 193 1502 5212 Fragments arranged by size: 5212 1502 193 EcoO109I rG'GnC_Cy Cuts at: 2056 2281 2320 2453 2553 3074 3955 4001 4084 Size: 225 39 133 100 521 881 46 83 Cuts at: 4084 4097 2056 Size: 13 4866 Fragments arranged by size: 4866 881 521 225 133 100 83 46 39 13 EcoRI G'AATT_C Cuts at: 2337 4216 4594 2337 Size: 1879 378 4650 Fragments arranged by size: 4650 1879 378 FspI TGC'GCA Cuts at: 5413 5413 Size: 6907 HaeII r_GCGC'y Cuts at: 1678 2164 2227 2251 2306 2744 2754 2970 6054 Size: 486 63 24 55 438 10 216 3084 Cuts at: 6054 1678 Size: 2531 Fragments arranged by size: 3084 2531 486 438 216 63 55 24 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3374 3406 3944 3976 5548 Size: 32 135 32 1802 32 538 32 1572 Cuts at: 5548 5580 5622 5654 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1802 1572 538 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2929 3064 3133 4251 4497 669 Size: 988 281 991 135 69 1118 246 3079 Fragments arranged by size: 3079 1118 991 988 281 246 135 69 HindIII A'AGCT_T Cuts at: 748 2407 4023 748 Size: 1659 1616 3632 Fragments arranged by size: 3632 1659 1616 HpaI GTT'AAC Cuts at: 4497 4497 Size: 6907 KasI G'GCGC_C Cuts at: 2247 2740 2247 Size: 493 6414 KpnI G_GTAC'C Cuts at: 4232 4232 Size: 6907 MfeI C'AATT_G Cuts at: 4506 4506 Size: 6907 MluI A'CGCG_T Cuts at: 6783 6783 Size: 6907 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6021 6205 1305 Size: 4716 184 2007 Fragments arranged by size: 4716 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3170 3783 10 Size: 54 1425 343 1338 613 3134 Fragments arranged by size: 3134 1425 1338 613 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2786 3479 4865 5224 5383 518 Size: 648 1620 693 1386 359 159 2042 Fragments arranged by size: 2042 1620 1386 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 2189 2215 2269 2650 2869 3103 3237 Size: 570 13 26 54 381 219 234 134 Cuts at: 3237 3916 4090 4118 4188 4323 5000 6148 6393 Size: 679 174 28 70 135 677 1148 245 Cuts at: 6393 1606 Size: 2120 Fragments arranged by size: 2120 1148 679 677 570 381 245 234 219 174 135 134 70 54 28 26 13 NaeI GCC'GGC Cuts at: 2228 2228 Size: 6907 NarI GG'CG_CC Cuts at: 2248 2741 2248 Size: 493 6414 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6355 NdeI CA'TA_TG Cuts at: 387 387 Size: 6907 NgoMIV G'CCGG_C Cuts at: 2226 2226 Size: 6907 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6907 NotI GC'GGCC_GC Cuts at: 4354 4354 Size: 6907 NruI TCG'CGA Cuts at: 3964 3964 Size: 6907 NspI r_CATG'y Cuts at: 1132 1229 2540 4265 5810 1132 Size: 97 1311 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 1311 97 PasI CC'CwG_GG Cuts at: 1348 2060 3044 3196 3667 1348 Size: 712 984 152 471 4588 Fragments arranged by size: 4588 984 712 471 152 PciI A'CATG_T Cuts at: 5806 5806 Size: 6907 PflMI CCAn_nnn'nTGG Cuts at: 1108 2976 3802 4064 1108 Size: 1868 826 262 3951 Fragments arranged by size: 3951 1868 826 262 PfoI T'CCnGG_A Cuts at: 3747 3747 Size: 6907 PmeI GTTT'AAAC Cuts at: 4211 4211 Size: 6907 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4976 5008 6489 6521 4976 Size: 32 1481 32 5362 Fragments arranged by size: 5362 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3074 3955 3074 Size: 881 6026 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6907 PsiI TTA'TAA Cuts at: 4477 4477 Size: 6907 PspOMI G'GGCC_C Cuts at: 2055 2889 4000 4096 2055 Size: 834 1111 96 4866 Fragments arranged by size: 4866 1111 834 96 PstI C_TGCA'G Cuts at: 830 3661 4259 830 Size: 2831 598 3478 Fragments arranged by size: 3478 2831 598 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6907 PvuII CAG'CTG Cuts at: 2176 2869 3103 3237 3916 2176 Size: 693 234 134 679 5167 Fragments arranged by size: 5167 693 679 234 134 SacII CC_GC'GG Cuts at: 2190 4091 4119 2190 Size: 1901 28 4978 Fragments arranged by size: 4978 1901 28 SalI G'TCGA_C Cuts at: 1936 4249 1936 Size: 2313 4594 SanDI GG'GwC_CC Cuts at: 3074 3074 Size: 6907 SbfI CC_TGCA'GG Cuts at: 4259 4259 Size: 6907 ScaI AGT'ACT Cuts at: 2990 5155 6867 2990 Size: 2165 1712 3030 Fragments arranged by size: 3030 2165 1712 SexAI A'CCwGG_T Cuts at: 3945 3945 Size: 6907 SfcI C'TryA_G Cuts at: 826 1045 3657 4255 5390 6071 6262 826 Size: 219 2612 598 1135 681 191 1471 Fragments arranged by size: 2612 1471 1135 681 598 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2897 2897 Size: 6907 SfoI GGC'GCC Cuts at: 2249 2742 2249 Size: 493 6414 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6907 SgrAI Cr'CCGG_yG Cuts at: 1700 2226 1700 Size: 526 6381 SmaI CCC'GGG Cuts at: 1872 2279 2888 4074 4234 1872 Size: 407 609 1186 160 4545 Fragments arranged by size: 4545 1186 609 407 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 5011 5912 6174 820 Size: 197 926 3068 901 262 1553 Fragments arranged by size: 3068 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4204 4698 493 Size: 3711 494 2702 Fragments arranged by size: 3711 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6907 SphI G_CATG'C Cuts at: 1132 4265 1132 Size: 3133 3774 SspI AAT'ATT Cuts at: 5 52 4831 5 Size: 47 4779 2081 Fragments arranged by size: 4779 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6907 StyI C'CwwG_G Cuts at: 513 1065 2284 2754 3763 4346 513 Size: 552 1219 470 1009 583 3074 Fragments arranged by size: 3074 1219 1009 583 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2331 4934 4951 5104 5289 5734 325 Size: 1255 751 2603 17 153 185 445 1498 Fragments arranged by size: 2603 1498 1255 751 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 2988 3303 Size: 274 80 33 51 1443 639 372 315 Cuts at: 3303 3555 5153 6865 96 Size: 252 1598 1712 138 Fragments arranged by size: 1712 1598 1443 639 372 315 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 6867 6899 2069 Size: 32 4766 32 2077 Fragments arranged by size: 4766 2077 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6907 XbaI T'CTAG_A Cuts at: 4243 4243 Size: 6907 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2501 3445 2501 Size: 944 5963 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6907 XmaI C'CCGG_G Cuts at: 1870 2277 2886 4072 4232 1870 Size: 407 609 1186 160 4545 Fragments arranged by size: 4545 1186 609 407 160 XmnI GAAnn'nnTTC Cuts at: 5036 5036 Size: 6907 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4715 276 Size: 53 83 186 936 3181 2468 Fragments arranged by size: 3181 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SrfI SwaI