Restriction map for
pFN21ASDF0390
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I MaeII MamI MboII
MfeI MluI MroI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcsI 739 GAATTC [GA]^AATT[CT]
AfaI 621 GTAC GT^AC
ApaI 128 GGGCCC GGGCC^C
ApoI 739 GAATTC [GA]^AATT[CT]
AspI 586 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 647 TGCGCA TGC^GCA
BanI 149 GGCACC G^G[CT][GA]CC
BsgI 453 GTGCAG GTGCAG(16/14)
BsmAI 169 GTCTC GTCTC(1/5)
BsmI 114 GCATTC GAATGC(1/-1)
Bsp1407I 620 TGTACA T^GTACA
BspMI 723 GCAGGT ACCTGC(4/8)
BsrDI 470 GCAATG GCAATG(2/0)
BsrGI 620 TGTACA T^GTACA
DdeI 710 CTGAG C^T[ACGT]AG
DrdI 718 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 739 GAATTC G^AATTC
FspI 647 TGCGCA TGC^GCA
HaeII 510 GGCGCT [GA]GCGC^[CT]
NcoI 278 CCATGG C^CATGG
PflFI 586 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 621 GTAC GT^AC
SfcI 753 CTACAG C^T[GA][CT]AG
SmaI 536 CCCGGG CCC^GGG
SspBI 620 TGTACA T^GTACA
TaqI 701 TCGA T^CGA
Tsp509I 740 AATT ^AATT
Tth111I 586 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 701 TCGA T^CGA
XmaCI 536 CCCGGG C^CCGGG
XmaI 536 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 310 CGCG CG^CG
613 CGCG
AcyI 262 GACGCC G[GA]^CG[CT]C
589 GGCGTC
BanII 128 GGGCCC G[GA]GC[CT]^C
244 GAGCCC
BfaI 160 CTAG C^TAG
355 CTAG
BglI 200 GCCCCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
535 GCCCGGGAGGC
BpmI 142 CTCCAG CTGGAG(16/14)
493 CTGGAG
BsaHI 262 GACGCC G[GA]^CG[CT]C
589 GGCGTC
BsrI 732 ACTGG ACTGG(1/-1)
809 CCAGT
BstUI 310 CGCG CG^CG
613 CGCG
EcoT14I 278 CCATGG C^C[AT][AT]GG
641 CCAAGG
Hin1I 262 GACGCC G[GA]^CG[CT]C
589 GGCGTC
HinfI 213 GACTC G^A[ACGT]TC
703 GACTC
KspI 309 CCGCGG CCGC^GG
612 CCGCGG
MaeI 160 CTAG C^TAG
355 CTAG
MaeIII 595 GTGAC ^GT[ACGT]AC
727 GTCAC
MvnI 310 CGCG CG^CG
613 CGCG
PflMI 327 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
809 CCAGTGCCTGG
PleI 213 GACTC GAGTC(4/5)
703 GACTC
SacII 309 CCGCGG CCGC^GG
612 CCGCGG
SfaNI 271 GATGC GCATC(5/9)
323 GCATC
StyI 278 CCATGG C^C[AT][AT]GG
641 CCAAGG
Tsp45I 595 GTGAC ^GT[GC]AC
727 GTCAC
Van91I 327 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
809 CCAGTGCCTGG
XcmI 248 CCAGACCAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
410 CCAAACTCGGGCTGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
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AciI AluI AlwI AlwNI AvaI
AvaII BalI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
MboI MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI TseI TspRI XhoII