(Circular) (Six-base) MAPSORT of: pFN21ASDF0390.seq Check: 4663 from: 1 to: 5554 pFN21ASDF0390 5554 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3364 278 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2897 1937 Size: 960 4594 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3617 5001 5269 1632 Size: 1985 1384 268 1917 Fragments arranged by size: 1985 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 4453 5430 1203 Size: 3250 977 1327 Fragments arranged by size: 3250 1327 977 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5554 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5554 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2798 2830 2798 Size: 32 5522 AlwNI CAG_nnn'CTG Cuts at: 2059 2326 2356 2428 2838 3297 4869 2059 Size: 267 30 72 410 459 1572 2744 Fragments arranged by size: 2744 1572 459 410 267 72 30 ApaI G_GGCC'C Cuts at: 2155 2155 Size: 5554 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2762 2863 3089 3241 5350 1493 Size: 1269 101 226 152 2109 1697 Fragments arranged by size: 2109 1697 1269 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2341 2438 2559 2879 1870 Size: 73 398 97 121 320 4545 Fragments arranged by size: 4545 398 320 121 97 73 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2172 2875 4330 618 Size: 325 217 627 385 703 1455 1842 Fragments arranged by size: 1842 1455 703 627 385 325 217 BanII G_rGCy'C Cuts at: 1391 2155 2271 2873 1391 Size: 764 116 602 4072 Fragments arranged by size: 4072 764 602 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3745 3779 1903 Size: 34 1808 34 3678 Fragments arranged by size: 3678 1808 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2229 2564 4165 Size: 107 122 71 631 605 557 335 1601 Cuts at: 4165 136 Size: 1525 Fragments arranged by size: 1601 1525 631 605 557 335 122 107 71 BglII A'GATC_T Cuts at: 5549 5549 Size: 5554 BlpI GC'TnA_GC Cuts at: 2971 2971 Size: 5554 Bme1580I G_kGCm'C Cuts at: 1790 2155 2320 2659 3615 4771 1790 Size: 365 165 339 956 1156 2573 Fragments arranged by size: 2573 1156 956 365 339 165 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3285 4243 442 Size: 416 53 83 399 24 1868 958 1753 Fragments arranged by size: 1868 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2537 4214 1239 Size: 911 387 1677 2579 Fragments arranged by size: 2579 1677 911 387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3679 4544 4842 3679 Size: 865 298 4391 Fragments arranged by size: 4391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4217 882 Size: 617 2718 2219 Fragments arranged by size: 2718 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2851 3345 493 Size: 711 1647 494 2702 Fragments arranged by size: 2702 1647 711 494 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2286 2613 3361 3743 Size: 53 83 186 936 753 327 748 382 Cuts at: 3743 4378 275 Size: 635 1451 Fragments arranged by size: 1451 936 753 748 635 382 327 186 83 53 BsaWI w'CCGG_w Cuts at: 3987 4385 4659 4806 5217 3987 Size: 398 274 147 411 4324 Fragments arranged by size: 4324 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2148 2178 2711 2741 1178 Size: 30 165 30 745 30 533 30 3991 Fragments arranged by size: 3991 745 533 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2534 2719 2825 1186 Size: 1348 185 106 3915 Fragments arranged by size: 3915 1348 185 106 BseYI C'CCAG_C Cuts at: 2091 2126 2148 4757 5253 2091 Size: 35 22 2609 496 2392 Fragments arranged by size: 2609 2392 496 35 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2497 1163 Size: 573 761 4220 Fragments arranged by size: 4220 761 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3004 3765 4793 1540 Size: 275 186 1003 761 1028 2301 Fragments arranged by size: 2301 1028 1003 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3615 3700 4771 3615 Size: 85 1071 4398 Fragments arranged by size: 4398 1071 85 BsmI GAATG_Cn' Cuts at: 2137 3065 3158 2137 Size: 928 93 4533 Fragments arranged by size: 4533 928 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2271 2320 2659 2873 3615 3700 Size: 399 365 116 49 339 214 742 85 Cuts at: 3700 4771 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 365 339 214 116 85 49 BspEI T'CCGG_A Cuts at: 5217 5217 Size: 5554 BspHI T'CATG_A Cuts at: 3443 5173 3443 Size: 1730 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2737 2909 844 Size: 1893 172 3489 Fragments arranged by size: 3489 1893 172 BsrBI CCG'CTC Cuts at: 1394 1823 3441 1394 Size: 429 1618 3507 Fragments arranged by size: 3507 1618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2500 4049 4223 65 Size: 1183 1252 1549 174 1396 Fragments arranged by size: 1549 1396 1252 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4198 4385 1700 Size: 110 2388 187 2869 Fragments arranged by size: 2869 2388 187 110 BsrGI T'GTAC_A Cuts at: 96 2643 96 Size: 2547 3007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5554 BssSI C'ACGA_G Cuts at: 3614 4626 3614 Size: 1012 4542 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5554 BstBI TT'CG_AA Cuts at: 2007 4358 2007 Size: 2351 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstYI r'GATC_y Cuts at: 1972 2038 2396 2512 2884 3251 3636 3653 5105 Size: 66 358 116 372 367 385 17 1452 Cuts at: 5105 5191 5203 5549 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 372 367 358 346 116 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 2289 2301 2332 2635 513 Size: 552 1224 12 31 303 3432 Fragments arranged by size: 3432 1224 552 303 31 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2557 487 Size: 683 56 1331 3484 Fragments arranged by size: 3484 1331 683 56 BtsI GCAGTG_nn' Cuts at: 2033 2120 2608 2680 3070 3864 3884 2033 Size: 87 488 72 390 794 20 3703 Fragments arranged by size: 3703 794 488 390 87 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2747 4561 809 Size: 507 1431 1814 1802 Fragments arranged by size: 1814 1802 1431 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 2430 2650 Size: 54 1425 50 275 18 528 72 220 Cuts at: 2650 2820 3001 3890 5519 8 Size: 170 181 889 1629 43 Fragments arranged by size: 1629 1425 889 528 275 220 181 170 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 1537 Size: 275 1189 4090 Fragments arranged by size: 4090 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3484 1353 Size: 2131 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2360 2474 2774 4141 4515 4661 Size: 1064 138 771 114 300 1367 374 146 Cuts at: 4661 387 Size: 1280 Fragments arranged by size: 1367 1280 1064 771 374 300 146 138 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2537 3617 4214 5001 5269 1239 Size: 393 518 387 1080 597 787 268 1524 Fragments arranged by size: 1524 1080 787 597 518 393 387 268 EcoO109I rG'GnC_Cy Cuts at: 2056 2152 2222 2337 2353 2056 Size: 96 70 115 16 5257 Fragments arranged by size: 5257 115 96 70 16 EcoRI G'AATT_C Cuts at: 2762 2863 3241 2762 Size: 101 378 5075 Fragments arranged by size: 5075 378 101 FspI TGC'GCA Cuts at: 2672 4060 2672 Size: 1388 4166 HaeII r_GCGC'y Cuts at: 1678 2537 4701 1678 Size: 859 2164 2531 Fragments arranged by size: 2531 2164 859 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2497 2529 4195 4227 4269 Size: 32 135 32 925 32 1666 32 42 Cuts at: 4269 4301 1373 Size: 32 2626 Fragments arranged by size: 2626 1666 925 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4668 4852 1305 Size: 3363 184 2007 Fragments arranged by size: 3363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 2432 2822 10 Size: 54 1425 343 528 72 390 2742 Fragments arranged by size: 2742 1425 528 390 343 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 3512 3871 4030 518 Size: 648 2346 359 159 2042 Fragments arranged by size: 2346 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2148 2259 2334 2502 2579 2637 2781 2845 Size: 542 111 75 168 77 58 144 64 Cuts at: 2845 2970 3647 4795 5040 1606 Size: 125 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 542 245 168 144 125 111 77 75 64 58 NcoI C'CATG_G Cuts at: 513 1065 2301 513 Size: 552 1236 3766 Fragments arranged by size: 3766 1236 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2912 4457 1132 Size: 97 1683 1545 2229 Fragments arranged by size: 2229 1683 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5554 PciI A'CATG_T Cuts at: 4453 4453 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 1108 2356 2838 1108 Size: 1248 482 3824 Fragments arranged by size: 3824 1248 482 PfoI T'CCnGG_A Cuts at: 2712 2766 2712 Size: 54 5500 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 5136 5168 3623 Size: 32 1481 32 4009 Fragments arranged by size: 4009 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5554 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PspOMI G'GGCC_C Cuts at: 2151 2151 Size: 5554 PstI C_TGCA'G Cuts at: 830 2906 830 Size: 2076 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 PvuII CAG'CTG Cuts at: 2148 2259 2579 2845 2148 Size: 111 320 266 4857 Fragments arranged by size: 4857 320 266 111 SacII CC_GC'GG Cuts at: 2335 2638 2335 Size: 303 5251 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 3802 5514 3802 Size: 1712 3842 SfcI C'TryA_G Cuts at: 826 1045 2776 2902 4037 4718 4909 826 Size: 219 1731 126 1135 681 191 1471 Fragments arranged by size: 1731 1471 1135 681 219 191 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2561 2881 1872 Size: 689 320 4545 Fragments arranged by size: 4545 689 320 SmlI C'TyrA_G Cuts at: 820 1017 1943 3658 4559 4821 820 Size: 197 926 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2851 3345 493 Size: 2358 494 2702 Fragments arranged by size: 2702 2358 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5554 StyI C'CwwG_G Cuts at: 513 1065 2301 2664 2993 513 Size: 552 1236 363 329 3074 Fragments arranged by size: 3074 1236 552 363 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2115 2702 3581 3598 3751 3936 4381 Size: 1255 535 587 879 17 153 185 445 Cuts at: 4381 325 Size: 1498 Fragments arranged by size: 1498 1255 879 587 535 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2643 3800 5512 Size: 274 80 33 51 1443 666 1157 1712 Cuts at: 5512 96 Size: 138 Fragments arranged by size: 1712 1443 1157 666 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2148 2180 5514 5546 2148 Size: 32 3334 32 2156 Fragments arranged by size: 3334 2156 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2612 1316 Size: 1296 4258 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2278 2440 2278 Size: 162 5392 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2559 2879 1870 Size: 689 320 4545 Fragments arranged by size: 4545 689 320 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3362 276 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI