Restriction map for
pFN21ASDF0390
(Linear) (Six-base) MAPSORT of: FLJ00390.seq Check: 4064 from: 1 to: 825
FLJ00390.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 825
Size: 775 32 18
Fragments arranged by size:
775 32 18
AlwNI CAG_nnn'CTG
Cuts at: 0 36 303 333 405 815 825
Size: 36 267 30 72 410 10
Fragments arranged by size:
410 267 72 36 30 10
ApaI G_GGCC'C
Cuts at: 0 132 825
Size: 132 693
ApoI r'AATT_y
Cuts at: 0 739 825
Size: 739 86
AvaI C'yCGr_G
Cuts at: 0 318 415 536 825
Size: 318 97 121 289
Fragments arranged by size:
318 289 121 97
BanI G'GyrC_C
Cuts at: 0 149 825
Size: 149 676
BanII G_rGCy'C
Cuts at: 0 132 248 825
Size: 132 116 577
Fragments arranged by size:
577 132 116
BglI GCCn_nnn'nGGC
Cuts at: 0 206 541 825
Size: 206 335 284
Fragments arranged by size:
335 284 206
Bme1580I G_kGCm'C
Cuts at: 0 132 297 636 825
Size: 132 165 339 189
Fragments arranged by size:
339 189 165 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 514 825
Size: 127 387 311
Fragments arranged by size:
387 311 127
BsaHI Gr'CG_yC
Cuts at: 0 263 590 825
Size: 263 327 235
Fragments arranged by size:
327 263 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 125 155 688 718 825
Size: 125 30 533 30 107
Fragments arranged by size:
533 125 107 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 696 802 825
Size: 511 185 106 23
Fragments arranged by size:
511 185 106 23
BseYI C'CCAG_C
Cuts at: 0 68 103 125 825
Size: 68 35 22 700
Fragments arranged by size:
700 68 35 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 825
Size: 474 351
BsmI GAATG_Cn'
Cuts at: 0 114 825
Size: 114 711
Bsp1286I G_dGCh'C
Cuts at: 0 132 248 297 636 825
Size: 132 116 49 339 189
Fragments arranged by size:
339 189 132 116 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 714 825
Size: 714 111
BsrDI GCAATG_nn'
Cuts at: 0 477 825
Size: 477 348
BsrGI T'GTAC_A
Cuts at: 0 620 825
Size: 620 205
BstYI r'GATC_y
Cuts at: 0 15 373 489 825
Size: 15 358 116 336
Fragments arranged by size:
358 336 116 15
BtgI C'CryG_G
Cuts at: 0 266 278 309 612 825
Size: 266 12 31 303 213
Fragments arranged by size:
303 266 213 31 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 534 825
Size: 534 291
BtsI GCAGTG_nn'
Cuts at: 0 10 97 585 657 825
Size: 10 87 488 72 168
Fragments arranged by size:
488 168 87 72 10
DrdI GACnn_nn'nnGTC
Cuts at: 0 724 825
Size: 724 101
EaeI y'GGCC_r
Cuts at: 0 335 407 627 797 825
Size: 335 72 220 170 28
Fragments arranged by size:
335 220 170 72 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 337 451 751 825
Size: 337 114 300 74
Fragments arranged by size:
337 300 114 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 514 825
Size: 127 387 311
Fragments arranged by size:
387 311 127
EcoO109I rG'GnC_Cy
Cuts at: 0 33 129 199 314 330 825
Size: 33 96 70 115 16 495
Fragments arranged by size:
495 115 96 70 33 16
EcoRI G'AATT_C
Cuts at: 0 739 825
Size: 739 86
FspI TGC'GCA
Cuts at: 0 649 825
Size: 649 176
HaeII r_GCGC'y
Cuts at: 0 514 825
Size: 514 311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 474 506 825
Size: 474 32 319
Fragments arranged by size:
474 319 32
MscI TGG'CCA
Cuts at: 0 337 409 799 825
Size: 337 72 390 26
Fragments arranged by size:
390 337 72 26
MspA1I CmG'CkG
Cuts at: 0 125 236 311 479 556 614 758 822
Size: 125 111 75 168 77 58 144 64
Cuts at: 822 825
Size: 3
Fragments arranged by size:
168 144 125 111 77 75 64 58
3
NcoI C'CATG_G
Cuts at: 0 278 825
Size: 278 547
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 815 825
Size: 333 482 10
Fragments arranged by size:
482 333 10
PfoI T'CCnGG_A
Cuts at: 0 689 743 825
Size: 689 54 82
Fragments arranged by size:
689 82 54
PspOMI G'GGCC_C
Cuts at: 0 128 825
Size: 128 697
PvuII CAG'CTG
Cuts at: 0 125 236 556 822 825
Size: 125 111 320 266 3
Fragments arranged by size:
320 266 125 111 3
SacII CC_GC'GG
Cuts at: 0 312 615 825
Size: 312 303 210
Fragments arranged by size:
312 303 210
SfcI C'TryA_G
Cuts at: 0 753 825
Size: 753 72
SmaI CCC'GGG
Cuts at: 0 538 825
Size: 538 287
StyI C'CwwG_G
Cuts at: 0 278 641 825
Size: 278 363 184
Fragments arranged by size:
363 278 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 679 825
Size: 92 587 146
Fragments arranged by size:
587 146 92
TatI w'GTAC_w
Cuts at: 0 620 825
Size: 620 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 825
Size: 125 32 668
Fragments arranged by size:
668 125 32
Tth111I GACn'n_nGTC
Cuts at: 0 589 825
Size: 589 236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 417 825
Size: 255 162 408
Fragments arranged by size:
408 255 162
XmaI C'CCGG_G
Cuts at: 0 536 825
Size: 536 289
Enzymes that do cut:
AloI AlwNI ApaI ApoI AvaI BanI BanII BglI
Bme1580I BpmI BsaHI BsaXI BseRI BseYI BsgI BsmI
Bsp1286I BspMI BsrDI BsrGI BstYI BtgI BtgZI BtsI
DrdI EaeI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I MscI MspA1I NcoI PflMI PfoI PspOMI PvuII
SacII SfcI SmaI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII EagI EarI EcoICRI EcoNI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XhoI
XmnI ZraI