(Circular) (Six-base) MAPSORT of: pFN21ASDF0380.seq Check: 4517 from: 1 to: 7045 pFN21ASDF0380 7045 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2819 2819 Size: 7045 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4855 278 Size: 53 83 186 936 3319 2468 Fragments arranged by size: 3319 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4388 1937 Size: 2451 4594 Acc65I G'GTAC_C Cuts at: 4366 4366 Size: 7045 AclI AA'CG_TT Cuts at: 1563 5172 5545 1563 Size: 3609 373 3063 Fragments arranged by size: 3609 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 3139 3522 5108 6492 6760 1632 Size: 422 1085 383 1586 1384 268 1917 Fragments arranged by size: 1917 1586 1384 1085 422 383 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6848 AflIII A'CryG_T Cuts at: 1203 5944 6921 1203 Size: 4741 977 1327 Fragments arranged by size: 4741 1327 977 AgeI A'CCGG_T Cuts at: 5876 5876 Size: 7045 AhdI GACnn_n'nnGTC Cuts at: 5774 5774 Size: 7045 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7045 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7045 AlwNI CAG_nnn'CTG Cuts at: 4171 4788 6360 4171 Size: 617 1572 4856 Fragments arranged by size: 4856 1572 617 ApaI G_GGCC'C Cuts at: 2160 2290 2160 Size: 130 6915 ApaLI G'TGCA_C Cuts at: 5102 6258 5102 Size: 1156 5889 ApoI r'AATT_y Cuts at: 1493 3437 4354 4580 4732 6841 1493 Size: 1944 917 226 152 2109 1697 Fragments arranged by size: 2109 1944 1697 917 226 152 AseI AT'TA_AT Cuts at: 160 1032 5599 160 Size: 872 4567 1606 Fragments arranged by size: 4567 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 AvaI C'yCGr_G Cuts at: 1870 1943 2393 3161 3876 4370 1870 Size: 73 450 768 715 494 4545 Fragments arranged by size: 4545 768 715 494 450 73 BamHI G'GATC_C Cuts at: 3694 4375 4742 3694 Size: 681 367 5997 Fragments arranged by size: 5997 681 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2746 3760 3999 4366 Size: 325 217 627 518 441 1014 239 367 Cuts at: 4366 5821 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1014 627 518 441 367 325 239 217 BanII G_rGCy'C Cuts at: 1391 2160 2290 2889 3270 3877 4364 1391 Size: 769 130 599 381 607 487 4072 Fragments arranged by size: 4072 769 607 599 487 381 130 BbeI G_GCGC'C Cuts at: 4003 4003 Size: 7045 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6074 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5236 5270 1903 Size: 34 3299 34 3678 Fragments arranged by size: 3678 3299 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2101 2231 4937 6147 2101 Size: 130 2706 1210 2999 Fragments arranged by size: 2999 2706 1210 130 BclI T'GATC_A Cuts at: 1548 1548 Size: 7045 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5656 136 Size: 107 122 71 631 605 3984 1525 Fragments arranged by size: 3984 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2539 2968 7040 2539 Size: 429 4072 2544 Fragments arranged by size: 4072 2544 429 BlpI GC'TnA_GC Cuts at: 3702 3977 4462 3702 Size: 275 485 6285 Fragments arranged by size: 6285 485 275 Bme1580I G_kGCm'C Cuts at: 1790 2160 2290 3040 3772 3805 5106 6262 1790 Size: 370 130 750 732 33 1301 1156 2573 Fragments arranged by size: 2573 1301 1156 750 732 370 130 33 BmgBI CAC'GTC Cuts at: 1318 2104 1318 Size: 786 6259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2154 2176 2501 Size: 416 53 83 399 24 737 22 325 Cuts at: 2501 2733 3759 3792 4221 4776 5734 442 Size: 232 1026 33 429 555 958 1753 Fragments arranged by size: 1753 1026 958 737 555 429 416 399 325 232 83 53 33 24 22 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 3066 3449 3454 3746 4088 4226 5705 Size: 806 1021 383 5 292 342 138 1479 Cuts at: 5705 1239 Size: 2579 Fragments arranged by size: 2579 1479 1021 806 383 342 292 138 5 Bpu10I CC'TnA_GC Cuts at: 3491 3491 Size: 7045 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3791 5170 6035 6333 3791 Size: 1379 865 298 4503 Fragments arranged by size: 4503 1379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2337 3042 3608 5708 882 Size: 617 838 705 566 2100 2219 Fragments arranged by size: 2219 2100 838 705 617 566 BsaAI yAC'GTr Cuts at: 493 1204 3733 4342 4836 493 Size: 711 2529 609 494 2702 Fragments arranged by size: 2702 2529 711 609 494 BsaBI GATnn'nnATC Cuts at: 2549 2571 7039 2549 Size: 22 4468 2555 Fragments arranged by size: 4468 2555 22 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3119 4000 4852 5234 Size: 53 83 186 936 1586 881 852 382 Cuts at: 5234 5869 275 Size: 635 1451 Fragments arranged by size: 1586 1451 936 881 852 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4329 5478 5876 6150 6297 6708 4329 Size: 1149 398 274 147 411 4666 Fragments arranged by size: 4666 1149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6820 Fragments arranged by size: 6820 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2907 3446 3517 3520 3944 4259 1186 Size: 1075 646 539 71 3 424 315 3972 Fragments arranged by size: 3972 1075 646 539 424 315 71 3 BseYI C'CCAG_C Cuts at: 2582 3215 3282 3423 3972 6248 6744 2582 Size: 633 67 141 549 2276 496 2883 Fragments arranged by size: 2883 2276 633 549 496 141 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2724 3434 1163 Size: 573 988 710 4774 Fragments arranged by size: 4774 988 710 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4495 5256 6284 1540 Size: 275 186 2494 761 1028 2301 Fragments arranged by size: 2494 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3062 3732 3870 5106 5191 6262 3062 Size: 670 138 1236 85 1071 3845 Fragments arranged by size: 3845 1236 1071 670 138 85 BsmI GAATG_Cn' Cuts at: 2833 4556 4649 2833 Size: 1723 93 5229 Fragments arranged by size: 5229 1723 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2290 2889 3040 3062 3270 3732 Size: 399 370 130 599 151 22 208 462 Cuts at: 3732 3772 3805 3870 3877 4364 5106 5191 6262 Size: 40 33 65 7 487 742 85 1071 Cuts at: 6262 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 599 487 462 399 370 208 151 130 85 65 40 33 22 7 BspEI T'CCGG_A Cuts at: 6708 6708 Size: 7045 BspHI T'CATG_A Cuts at: 4934 6664 4934 Size: 1730 5315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2712 2819 3795 3927 4095 4400 844 Size: 1868 107 976 132 168 305 3489 Fragments arranged by size: 3489 1868 976 305 168 132 107 BsrBI CCG'CTC Cuts at: 1394 1823 4932 1394 Size: 429 3109 3507 Fragments arranged by size: 3507 3109 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5540 5714 65 Size: 1183 4292 174 1396 Fragments arranged by size: 4292 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5689 5876 1700 Size: 110 3879 187 2869 Fragments arranged by size: 3879 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3030 96 Size: 2934 4111 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7045 BssSI C'ACGA_G Cuts at: 5105 6117 5105 Size: 1012 6033 BstAPI GCAn_nnn'nTGC Cuts at: 794 3767 794 Size: 2973 4072 BstBI TT'CG_AA Cuts at: 2007 5849 2007 Size: 3842 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7045 BstXI CCAn_nnnn'nTGG Cuts at: 3290 3772 3290 Size: 482 6563 BstYI r'GATC_y Cuts at: 1972 2539 2968 3622 3694 3888 4375 4742 5127 Size: 567 429 654 72 194 487 367 385 Cuts at: 5127 5144 6596 6682 6694 7040 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 654 567 487 429 385 367 346 194 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 2195 2792 2195 Size: 597 6448 BtgI C'CryG_G Cuts at: 513 1065 2997 513 Size: 552 1932 4561 Fragments arranged by size: 4561 1932 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6306 Fragments arranged by size: 6306 683 56 BtsI GCAGTG_nn' Cuts at: 2256 2874 2966 4267 4561 5355 5375 2256 Size: 618 92 1301 294 794 20 3926 Fragments arranged by size: 3926 1301 794 618 294 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7045 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7010 DraI TTT'AAA Cuts at: 4349 4349 Size: 7045 DraIII CAC_nnn'GTG Cuts at: 3767 3800 3767 Size: 33 7012 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2773 4262 6052 809 Size: 507 1457 1489 1790 1802 Fragments arranged by size: 1802 1790 1489 1457 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3183 3387 4287 Size: 54 1425 50 275 18 1353 204 900 Cuts at: 4287 4492 5381 7010 8 Size: 205 889 1629 43 Fragments arranged by size: 1629 1425 1353 900 889 275 205 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4492 1537 Size: 275 2680 4090 Fragments arranged by size: 4090 2680 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2778 2901 2925 3158 3275 3523 3538 3544 Size: 1425 123 24 233 117 248 15 6 Cuts at: 3544 3571 3624 4975 1353 Size: 27 53 1351 3423 Fragments arranged by size: 3423 1425 1351 248 233 123 117 53 27 24 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5632 6006 6152 387 Size: 1064 138 4043 374 146 1280 Fragments arranged by size: 4043 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2054 3066 3139 3449 3454 3522 Size: 393 413 9 1012 73 310 5 68 Cuts at: 3522 3746 4088 4226 5108 5705 6492 6760 1239 Size: 224 342 138 882 597 787 268 1524 Fragments arranged by size: 1524 1012 882 787 597 413 393 342 310 268 224 138 73 68 9 5 EcoNI CCTnn'n_nnAGG Cuts at: 2276 3113 4175 2276 Size: 837 1062 5146 Fragments arranged by size: 5146 1062 837 EcoO109I rG'GnC_Cy Cuts at: 2156 2286 2287 4084 2156 Size: 130 1 1797 5117 Fragments arranged by size: 5117 1797 130 1 EcoRI G'AATT_C Cuts at: 4354 4732 4354 Size: 378 6667 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2059 2807 2059 Size: 748 6297 FspI TGC'GCA Cuts at: 5551 5551 Size: 7045 HaeII r_GCGC'y Cuts at: 1678 4003 6192 1678 Size: 2325 2189 2531 Fragments arranged by size: 2531 2325 2189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2960 2992 3434 3466 3607 Size: 32 135 32 1388 32 442 32 141 Cuts at: 3607 3639 5686 5718 5760 5792 1373 Size: 32 2047 32 42 32 2626 Fragments arranged by size: 2626 2047 1388 442 141 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4389 4635 669 Size: 988 281 2451 246 3079 Fragments arranged by size: 3079 2451 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7045 HpaI GTT'AAC Cuts at: 4635 4635 Size: 7045 KasI G'GCGC_C Cuts at: 3999 3999 Size: 7045 KpnI G_GTAC'C Cuts at: 4370 4370 Size: 7045 MfeI C'AATT_G Cuts at: 4644 4644 Size: 7045 MluI A'CGCG_T Cuts at: 6921 6921 Size: 7045 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3342 6159 6343 1305 Size: 2037 2817 184 2007 Fragments arranged by size: 2817 2037 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3185 3389 10 Size: 54 1425 343 1353 204 3666 Fragments arranged by size: 3666 1425 1353 343 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 2987 3002 5003 5362 5521 518 Size: 648 1821 15 2001 359 159 2042 Fragments arranged by size: 2042 2001 1821 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 3427 3976 4461 5138 6286 6531 1606 Size: 1821 549 485 677 1148 245 2120 Fragments arranged by size: 2120 1821 1148 677 549 485 245 NarI GG'CG_CC Cuts at: 4000 4000 Size: 7045 NcoI C'CATG_G Cuts at: 513 1065 2997 513 Size: 552 1932 4561 Fragments arranged by size: 4561 1932 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7045 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7045 NotI GC'GGCC_GC Cuts at: 4492 4492 Size: 7045 NspI r_CATG'y Cuts at: 1132 1229 4403 5948 1132 Size: 97 3174 1545 2229 Fragments arranged by size: 3174 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3124 1348 Size: 1776 5269 PciI A'CATG_T Cuts at: 5944 5944 Size: 7045 PflMI CCAn_nnn'nTGG Cuts at: 1108 2513 1108 Size: 1405 5640 PfoI T'CCnGG_A Cuts at: 2139 3043 3840 2139 Size: 904 797 5344 Fragments arranged by size: 5344 904 797 PmeI GTTT'AAAC Cuts at: 4349 4349 Size: 7045 PmlI CAC'GTG Cuts at: 3733 3733 Size: 7045 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5114 5146 6627 6659 5114 Size: 32 1481 32 5500 Fragments arranged by size: 5500 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4084 4084 Size: 7045 PshAI GACnn'nnGTC Cuts at: 1613 2604 1613 Size: 991 6054 PsiI TTA'TAA Cuts at: 4615 4615 Size: 7045 PspOMI G'GGCC_C Cuts at: 2156 2286 2156 Size: 130 6915 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3656 3688 3656 Size: 32 7013 PstI C_TGCA'G Cuts at: 830 3137 4092 4397 830 Size: 2307 955 305 3478 Fragments arranged by size: 3478 2307 955 305 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7045 PvuII CAG'CTG Cuts at: 3427 3976 3427 Size: 549 6496 SalI G'TCGA_C Cuts at: 1936 4387 1936 Size: 2451 4594 SapI GCTCTTCn'nnn_ Cuts at: 2901 3275 2901 Size: 374 6671 SbfI CC_TGCA'GG Cuts at: 4397 4397 Size: 7045 ScaI AGT'ACT Cuts at: 5293 7005 5293 Size: 1712 5333 SfcI C'TryA_G Cuts at: 826 1045 2496 2602 3067 3133 3598 4088 4393 Size: 219 1451 106 465 66 465 490 305 Cuts at: 4393 5528 6209 6400 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1451 1135 681 490 465 465 305 219 191 106 66 SfoI GGC'GCC Cuts at: 4001 4001 Size: 7045 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7045 SmaI CCC'GGG Cuts at: 1872 4372 1872 Size: 2500 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 3806 5149 6050 6312 820 Size: 197 926 450 1413 1343 901 262 1553 Fragments arranged by size: 1553 1413 1343 926 901 450 262 197 SnaBI TAC'GTA Cuts at: 493 4342 4836 493 Size: 3849 494 2702 Fragments arranged by size: 3849 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7045 SphI G_CATG'C Cuts at: 1132 4403 1132 Size: 3271 3774 SspI AAT'ATT Cuts at: 5 52 4969 5 Size: 47 4917 2081 Fragments arranged by size: 4917 2081 47 StuI AGG'CCT Cuts at: 1517 2385 3419 3456 1517 Size: 868 1034 37 5106 Fragments arranged by size: 5106 1034 868 37 StyI C'CwwG_G Cuts at: 513 1065 2205 2997 4484 513 Size: 552 1140 792 1487 3074 Fragments arranged by size: 3074 1487 1140 792 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3778 3811 5072 5089 5242 5427 5872 Size: 1255 2198 33 1261 17 153 185 445 Cuts at: 5872 325 Size: 1498 Fragments arranged by size: 2198 1498 1261 1255 445 185 153 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3030 5291 7003 Size: 274 80 33 51 1443 1053 2261 1712 Cuts at: 7003 96 Size: 138 Fragments arranged by size: 2261 1712 1443 1053 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2945 2977 7005 7037 2945 Size: 32 4028 32 2953 Fragments arranged by size: 4028 2953 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2935 1316 Size: 1619 5426 XbaI T'CTAG_A Cuts at: 4381 4381 Size: 7045 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4063 4184 4063 Size: 121 6924 XhoI C'TCGA_G Cuts at: 1943 2393 1943 Size: 450 6595 XmaI C'CCGG_G Cuts at: 1870 4370 1870 Size: 2500 4545 XmnI GAAnn'nnTTC Cuts at: 2055 5174 2055 Size: 3119 3926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4853 276 Size: 53 83 186 936 3319 2468 Fragments arranged by size: 3319 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI