Restriction map for
pFN21ASDF0201
(Linear) (Six-base) MAPSORT of: FLJ00201.seq Check: 9204 from: 1 to: 2109
FLJ00201.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1643 1846 2109
Size: 1643 203 263
Fragments arranged by size:
1643 263 203
AccI GT'mk_AC
Cuts at: 0 830 1856 2109
Size: 830 1026 253
Fragments arranged by size:
1026 830 253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1465 2109
Size: 244 1221 644
Fragments arranged by size:
1221 644 244
AflIII A'CryG_T
Cuts at: 0 1895 2109
Size: 1895 214
AleI CACnn'nnGTG
Cuts at: 0 1735 1894 2109
Size: 1735 159 215
Fragments arranged by size:
1735 215 159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 683 2109
Size: 683 1426
AlwNI CAG_nnn'CTG
Cuts at: 0 189 802 1147 1825 2109
Size: 189 613 345 678 284
Fragments arranged by size:
678 613 345 284 189
ApaI G_GGCC'C
Cuts at: 0 347 496 548 575 593 654 741 811
Size: 347 149 52 27 18 61 87 70
Cuts at: 811 880 916 985 1057 1121 1248 1274 1427
Size: 69 36 69 72 64 127 26 153
Cuts at: 1427 1472 1514 1523 1558 1577 2109
Size: 45 42 9 35 19 532
Fragments arranged by size:
532 347 153 149 127 87 72 70
69 69 64 61 52 45 42 36
35 27 26 19 18 9
ApaLI G'TGCA_C
Cuts at: 0 1876 2109
Size: 1876 233
ApoI r'AATT_y
Cuts at: 0 1777 2109
Size: 1777 332
AvaI C'yCGr_G
Cuts at: 0 253 260 489 637 908 1570 1578 1761
Size: 253 7 229 148 271 662 8 183
Cuts at: 1761 2109
Size: 348
Fragments arranged by size:
662 348 271 253 229 183 148 8
7
BanI G'GyrC_C
Cuts at: 0 526 537 667 1591 1657 1888 2109
Size: 526 11 130 924 66 231 221
Fragments arranged by size:
924 526 231 221 130 66 11
BanII G_rGCy'C
Cuts at: 0 236 347 389 496 548 575 593 654
Size: 236 111 42 107 52 27 18 61
Cuts at: 654 741 811 880 916 985 1057 1121 1248
Size: 87 70 69 36 69 72 64 127
Cuts at: 1248 1274 1329 1355 1427 1472 1514 1523 1558
Size: 26 55 26 72 45 42 9 35
Cuts at: 1558 1577 2109
Size: 19 532
Fragments arranged by size:
532 236 127 111 107 87 72 72
70 69 69 64 61 55 52 45
42 42 36 35 27 26 26 19
18 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 2109
Size: 17 2092
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1690 1724 2109
Size: 1690 34 385
Fragments arranged by size:
1690 385 34
BglI GCCn_nnn'nGGC
Cuts at: 0 100 281 411 491 500 683 938 1125
Size: 100 181 130 80 9 183 255 187
Cuts at: 1125 1439 1518 1572 1600 1723 1808 1847 2109
Size: 314 79 54 28 123 85 39 262
Fragments arranged by size:
314 262 255 187 183 181 130 123
100 85 80 79 54 39 28 9
Bme1580I G_kGCm'C
Cuts at: 0 347 496 531 548 575 593 654 672
Size: 347 149 35 17 27 18 61 18
Cuts at: 672 741 811 880 916 985 1057 1121 1248
Size: 69 70 69 36 69 72 64 127
Cuts at: 1248 1274 1427 1472 1514 1523 1558 1577 1596
Size: 26 153 45 42 9 35 19 19
Cuts at: 1596 1880 1891 2109
Size: 284 11 218
Fragments arranged by size:
347 284 218 153 149 127 72 70
69 69 69 64 61 45 42 36
35 35 27 26 19 19 18 18
17 11 9
BmgBI CAC'GTC
Cuts at: 0 1114 1881 2109
Size: 1114 767 228
Fragments arranged by size:
1114 767 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 112 1277 2109
Size: 112 1165 832
Fragments arranged by size:
1165 832 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 845 1169 1325 1384 1496 1978 2109
Size: 791 54 324 156 59 112 482 131
Fragments arranged by size:
791 482 324 156 131 112 59 54
Bpu10I CC'TnA_GC
Cuts at: 0 705 2109
Size: 705 1404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 2109
Size: 595 1514
BsaHI Gr'CG_yC
Cuts at: 0 76 1853 2109
Size: 76 1777 256
Fragments arranged by size:
1777 256 76
BsaWI w'CCGG_w
Cuts at: 0 1784 2109
Size: 1784 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1542 1572 2059 2089 2109
Size: 1542 30 487 30 20
Fragments arranged by size:
1542 487 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2067 2109
Size: 2067 42
BseYI C'CCAG_C
Cuts at: 0 3 57 340 984 988 1204 1507 1665
Size: 3 54 283 644 4 216 303 158
Cuts at: 1665 1695 1818 2109
Size: 30 123 291
Fragments arranged by size:
644 303 291 283 216 158 123 54
30 4 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2044 2109
Size: 2044 65
BsiHKAI G_wGCw'C
Cuts at: 0 1742 1880 1994 2109
Size: 1742 138 114 115
Fragments arranged by size:
1742 138 115 114
BsmI GAATG_Cn'
Cuts at: 0 1172 2109
Size: 1172 937
Bsp1286I G_dGCh'C
Cuts at: 0 236 347 389 496 531 548 575 593
Size: 236 111 42 107 35 17 27 18
Cuts at: 593 654 672 741 811 880 916 985 1057
Size: 61 18 69 70 69 36 69 72
Cuts at: 1057 1121 1248 1274 1329 1355 1427 1472 1514
Size: 64 127 26 55 26 72 45 42
Cuts at: 1514 1523 1558 1577 1596 1742 1880 1891 1994
Size: 9 35 19 19 146 138 11 103
Cuts at: 1994 2109
Size: 115
Fragments arranged by size:
236 146 138 127 115 111 107 103
72 72 70 69 69 69 64 61
55 45 42 42 36 35 35 27
26 26 19 19 18 18 17 11
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1643 1846 2109
Size: 1643 203 263
Fragments arranged by size:
1643 263 203
BsrFI r'CCGG_y
Cuts at: 0 94 1722 1926 2109
Size: 94 1628 204 183
Fragments arranged by size:
1628 204 183 94
BsrGI T'GTAC_A
Cuts at: 0 1910 2109
Size: 1910 199
BstEII G'GTnAC_C
Cuts at: 0 1180 2109
Size: 1180 929
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1978 2109
Size: 1978 131
Bsu36I CC'TnA_GG
Cuts at: 0 498 1130 1372 1383 2109
Size: 498 632 242 11 726
Fragments arranged by size:
726 632 498 242 11
BtgI C'CryG_G
Cuts at: 0 70 321 1248 1514 2060 2109
Size: 70 251 927 266 546 49
Fragments arranged by size:
927 546 266 251 70 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1605 2109
Size: 1605 504
BtsI GCAGTG_nn'
Cuts at: 0 1730 2109
Size: 1730 379
DrdI GACnn_nn'nnGTC
Cuts at: 0 21 1403 2109
Size: 21 1382 706
Fragments arranged by size:
1382 706 21
EaeI y'GGCC_r
Cuts at: 0 1800 1928 2109
Size: 1800 128 181
Fragments arranged by size:
1800 181 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 791 845 1169 1325 1384 1465 1496
Size: 244 547 54 324 156 59 81 31
Cuts at: 1496 1978 2109
Size: 482 131
Fragments arranged by size:
547 482 324 244 156 131 81 59
54 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1969 2109
Size: 1969 140
EcoO109I rG'GnC_Cy
Cuts at: 0 148 265 344 456 466 475 493 544
Size: 148 117 79 112 10 9 18 51
Cuts at: 544 545 571 572 590 650 651 737 738
Size: 1 26 1 18 60 1 86 1
Cuts at: 738 781 817 876 905 912 913 931 964
Size: 43 36 59 29 7 1 18 33
Cuts at: 964 981 993 1053 1072 1118 1144 1153 1189
Size: 17 12 60 19 46 26 9 36
Cuts at: 1189 1218 1244 1378 1424 1468 1469 1519 1520
Size: 29 26 134 46 44 1 50 1
Cuts at: 1520 1530 1555 1564 1574 1582 1601 2109
Size: 10 25 9 10 8 19 508
Fragments arranged by size:
508 148 134 117 112 86 79 60
60 59 51 50 46 46 44 43
36 36 33 29 29 26 26 26
25 19 19 18 18 18 17 12
10 10 10 9 9 9 8 7
1 1 1 1 1 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1960 1992 2081 2109
Size: 1960 32 89 28
Fragments arranged by size:
1960 89 32 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2015 2109
Size: 2015 94
MscI TGG'CCA
Cuts at: 0 1802 2109
Size: 1802 307
MslI CAynn'nnrTG
Cuts at: 0 1735 1771 1894 2109
Size: 1735 36 123 215
Fragments arranged by size:
1735 215 123 36
MspA1I CmG'CkG
Cuts at: 0 72 207 700 988 1411 1808 1998 2109
Size: 72 135 493 288 423 397 190 111
Fragments arranged by size:
493 423 397 288 190 135 111 72
NaeI GCC'GGC
Cuts at: 0 1928 2109
Size: 1928 181
NcoI C'CATG_G
Cuts at: 0 321 1248 2109
Size: 321 927 861
Fragments arranged by size:
927 861 321
NgoMIV G'CCGG_C
Cuts at: 0 1926 2109
Size: 1926 183
NspI r_CATG'y
Cuts at: 0 1770 2109
Size: 1770 339
PasI CC'CwG_GG
Cuts at: 0 244 382 576 655 785 847 919 1123
Size: 244 138 194 79 130 62 72 204
Cuts at: 1123 1329 1357 1525 1597 2109
Size: 206 28 168 72 512
Fragments arranged by size:
512 244 206 204 194 168 138 130
79 72 72 62 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 189 1690 1825 1898 2109
Size: 189 1501 135 73 211
Fragments arranged by size:
1501 211 189 135 73
PfoI T'CCnGG_A
Cuts at: 0 358 768 822 2109
Size: 358 410 54 1287
Fragments arranged by size:
1287 410 358 54
PpuMI rG'GwC_Cy
Cuts at: 0 148 265 466 475 817 905 993 1072
Size: 148 117 201 9 342 88 88 79
Cuts at: 1072 1153 1218 1378 1530 1582 2109
Size: 81 65 160 152 52 527
Fragments arranged by size:
527 342 201 160 152 148 117 88
88 81 79 65 52 9
PspOMI G'GGCC_C
Cuts at: 0 343 492 544 571 589 650 737 807
Size: 343 149 52 27 18 61 87 70
Cuts at: 807 876 912 981 1053 1117 1244 1270 1423
Size: 69 36 69 72 64 127 26 153
Cuts at: 1423 1468 1510 1519 1554 1573 2109
Size: 45 42 9 35 19 536
Fragments arranged by size:
536 343 153 149 127 87 72 70
69 69 64 61 52 45 42 36
35 27 26 19 18 9
PstI C_TGCA'G
Cuts at: 0 1098 2109
Size: 1098 1011
PvuII CAG'CTG
Cuts at: 0 700 988 1411 1998 2109
Size: 700 288 423 587 111
Fragments arranged by size:
700 587 423 288 111
SacII CC_GC'GG
Cuts at: 0 73 2109
Size: 73 2036
SanDI GG'GwC_CC
Cuts at: 0 265 466 905 993 1072 1218 1378 1530
Size: 265 201 439 88 79 146 160 152
Cuts at: 1530 1582 2109
Size: 52 527
Fragments arranged by size:
527 439 265 201 160 152 146 88
79 52
SexAI A'CCwGG_T
Cuts at: 0 522 714 2012 2109
Size: 522 192 1298 97
Fragments arranged by size:
1298 522 192 97
SfcI C'TryA_G
Cuts at: 0 1094 2109
Size: 1094 1015
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 491 683 1125 1439 1518 1572 2109
Size: 491 192 442 314 79 54 537
Fragments arranged by size:
537 491 442 314 192 79 54
SmaI CCC'GGG
Cuts at: 0 255 491 639 910 1572 1580 2109
Size: 255 236 148 271 662 8 529
Fragments arranged by size:
662 529 271 255 236 148 8
SmlI C'TyrA_G
Cuts at: 0 610 2109
Size: 610 1499
SphI G_CATG'C
Cuts at: 0 1770 2109
Size: 1770 339
SrfI GCCC'GGGC
Cuts at: 0 255 639 2109
Size: 255 384 1470
Fragments arranged by size:
1470 384 255
StuI AGG'CCT
Cuts at: 0 608 1433 1460 2109
Size: 608 825 27 649
Fragments arranged by size:
825 649 608 27
StyI C'CwwG_G
Cuts at: 0 321 531 968 1248 1274 1428 2109
Size: 321 210 437 280 26 154 681
Fragments arranged by size:
681 437 321 280 210 154 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1890 2010 2109
Size: 1890 120 99
Fragments arranged by size:
1890 120 99
TatI w'GTAC_w
Cuts at: 0 125 1910 1949 2066 2109
Size: 125 1785 39 117 43
Fragments arranged by size:
1785 125 117 43 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1301 1333 1542 1574 2109
Size: 1301 32 209 32 535
Fragments arranged by size:
1301 535 209 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2014 2109
Size: 2014 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 328 583 1192 1255 1408 1647 1899 1978
Size: 328 255 609 63 153 239 252 79
Cuts at: 1978 2109
Size: 131
Fragments arranged by size:
609 328 255 252 239 153 131 79
63
XmaI C'CCGG_G
Cuts at: 0 253 489 637 908 1570 1578 2109
Size: 253 236 148 271 662 8 531
Fragments arranged by size:
662 531 271 253 236 148 8
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BcgI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrFI BsrGI BstEII BstXI Bsu36I BtgI BtgZI BtsI
DrdI EaeI Eco57MI EcoNI EcoO109I Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacII SanDI SexAI
SfcI SfiI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BclI BfrBI BglII BlpI BmtI BplI BsaI
BsaAI BsaBI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstYI BstZ17I ClaI
CspCI DraI DraIII EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI XmnI ZraI