(Circular) (Six-base) MAPSORT of: pFN21ASDF0166.seq Check: 6256 from: 1 to: 6046 pFN21ASDF0166 6046 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 3301 2284 Size: 1017 5029 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 1937 Size: 1452 4594 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2721 2913 4109 5493 5761 1632 Size: 1089 192 1196 1384 268 1917 Fragments arranged by size: 1917 1384 1196 1089 268 192 AflII C'TTAA_G Cuts at: 820 1017 2521 820 Size: 197 1504 4345 Fragments arranged by size: 4345 1504 197 AflIII A'CryG_T Cuts at: 1203 4945 5922 1203 Size: 3742 977 1327 Fragments arranged by size: 3742 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 3789 5361 3789 Size: 1572 4474 ApaI G_GGCC'C Cuts at: 2156 2156 Size: 6046 ApaLI G'TGCA_C Cuts at: 4103 5259 4103 Size: 1156 4890 ApoI r'AATT_y Cuts at: 1493 2219 2435 2813 2884 3336 3355 3581 3733 Size: 726 216 378 71 452 19 226 152 Cuts at: 3733 5842 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 726 452 378 226 216 152 71 19 AseI AT'TA_AT Cuts at: 160 1032 2292 4600 160 Size: 872 1260 2308 1606 Fragments arranged by size: 2308 1606 1260 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2148 3371 1870 Size: 73 205 1223 4545 Fragments arranged by size: 4545 1223 205 73 BamHI G'GATC_C Cuts at: 2554 3376 3743 2554 Size: 822 367 4857 Fragments arranged by size: 4857 822 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3367 4822 618 Size: 325 217 627 1580 1455 1842 Fragments arranged by size: 1842 1580 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2156 3365 1391 Size: 765 1209 4072 Fragments arranged by size: 4072 1209 765 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2304 928 Size: 971 405 4670 Fragments arranged by size: 4670 971 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 2156 4107 5263 1790 Size: 366 1951 1156 2573 Fragments arranged by size: 2573 1951 1156 366 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3777 4735 442 Size: 416 53 83 399 24 2360 958 1753 Fragments arranged by size: 2360 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2633 1056 Size: 1577 4469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4706 1239 Size: 3467 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4709 882 Size: 617 3210 2219 Fragments arranged by size: 3210 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3343 3837 493 Size: 711 2139 494 2702 Fragments arranged by size: 2702 2139 711 494 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2986 3853 4235 4870 Size: 53 83 186 936 1453 867 382 635 Cuts at: 4870 275 Size: 1451 Fragments arranged by size: 1453 1451 936 867 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4479 4877 5151 5298 5709 4479 Size: 398 274 147 411 4816 Fragments arranged by size: 4816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5821 Fragments arranged by size: 5821 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3201 1186 Size: 2015 4031 BseYI C'CCAG_C Cuts at: 2163 5249 5745 2163 Size: 3086 496 2464 Fragments arranged by size: 3086 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5473 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 3496 4257 5285 1540 Size: 275 186 135 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 135 BsiHKAI G_wGCw'C Cuts at: 4107 4192 5263 4107 Size: 85 1071 4890 Fragments arranged by size: 4890 1071 85 BsmI GAATG_Cn' Cuts at: 3557 3650 3557 Size: 93 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 3365 4107 4192 5263 1391 Size: 399 366 1209 742 85 1071 2174 Fragments arranged by size: 2174 1209 1071 742 399 366 85 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 2478 3935 5665 2478 Size: 1457 1730 2859 Fragments arranged by size: 2859 1730 1457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2284 3301 3401 844 Size: 1440 1017 100 3489 Fragments arranged by size: 3489 1440 1017 100 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4690 4877 1700 Size: 110 2880 187 2869 Fragments arranged by size: 2880 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 3060 4106 5118 3060 Size: 1046 1012 3988 Fragments arranged by size: 3988 1046 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2173 3074 3281 794 Size: 1379 901 207 3559 Fragments arranged by size: 3559 1379 901 207 BstBI TT'CG_AA Cuts at: 2007 4850 2007 Size: 2843 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstYI r'GATC_y Cuts at: 1972 2239 2554 3376 3743 4128 4145 5597 5683 Size: 267 315 822 367 385 17 1452 86 Cuts at: 5683 5695 6041 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 822 385 367 346 315 267 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2059 2548 513 Size: 552 957 37 489 4011 Fragments arranged by size: 4011 957 552 489 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 3562 4356 4376 3562 Size: 794 20 5232 Fragments arranged by size: 5232 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 2946 2977 3219 3268 3350 2946 Size: 31 242 49 82 5642 Fragments arranged by size: 5642 242 82 49 31 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3493 4382 Size: 54 1425 50 275 18 303 1360 889 Cuts at: 4382 6011 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1360 889 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2133 3493 1537 Size: 275 321 1360 4090 Fragments arranged by size: 4090 1360 321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2656 2661 2697 3976 1353 Size: 833 470 5 36 1279 3423 Fragments arranged by size: 3423 1279 833 470 36 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2721 2913 4109 4706 5493 5761 1239 Size: 393 1089 192 1196 597 787 268 1524 Fragments arranged by size: 1524 1196 1089 787 597 393 268 192 EcoO109I rG'GnC_Cy Cuts at: 2032 2152 2032 Size: 120 5926 EcoRI G'AATT_C Cuts at: 2435 2813 3355 3733 2435 Size: 378 542 378 4748 Fragments arranged by size: 4748 542 378 378 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 5193 1678 Size: 3515 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 2583 2615 4687 Size: 32 135 32 974 32 5 32 2072 Cuts at: 4687 4719 4761 4793 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2072 974 135 42 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 2200 2968 3390 3636 669 Size: 988 281 262 768 422 246 3079 Fragments arranged by size: 3079 988 768 422 281 262 246 HindIII A'AGCT_T Cuts at: 748 3018 3048 3243 748 Size: 2270 30 195 3551 Fragments arranged by size: 3551 2270 195 30 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2962 3161 5160 5344 1305 Size: 1657 199 1999 184 2007 Fragments arranged by size: 2007 1999 1657 199 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4224 Fragments arranged by size: 4224 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2994 3147 4004 4363 4522 518 Size: 648 1828 153 857 359 159 2042 Fragments arranged by size: 2042 1828 857 648 359 159 153 MspA1I CmG'CkG Cuts at: 1606 3462 4139 5287 5532 1606 Size: 1856 677 1148 245 2120 Fragments arranged by size: 2120 1856 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2548 513 Size: 552 957 526 4011 Fragments arranged by size: 4011 957 552 526 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NheI G'CTAG_C Cuts at: 1052 2629 1052 Size: 1577 4469 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 3404 4949 1132 Size: 97 2175 1545 2229 Fragments arranged by size: 2229 2175 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6046 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PfoI T'CCnGG_A Cuts at: 2258 2800 2258 Size: 542 5504 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2032 Size: 6046 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspOMI G'GGCC_C Cuts at: 2152 2152 Size: 6046 PstI C_TGCA'G Cuts at: 830 3398 830 Size: 2568 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SapI GCTCTTCn'nnn_ Cuts at: 2656 2697 2656 Size: 41 6005 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2719 3394 4529 5210 5401 826 Size: 219 1674 675 1135 681 191 1471 Fragments arranged by size: 1674 1471 1135 681 675 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 2150 3373 1872 Size: 278 1223 4545 Fragments arranged by size: 4545 1223 278 SmlI C'TyrA_G Cuts at: 820 1017 1943 2521 4150 5051 5313 820 Size: 197 926 578 1629 901 262 1553 Fragments arranged by size: 1629 1553 926 901 578 262 197 SnaBI TAC'GTA Cuts at: 493 3343 3837 493 Size: 2850 494 2702 Fragments arranged by size: 2850 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 2871 1517 Size: 1354 4692 StyI C'CwwG_G Cuts at: 513 1065 2022 2548 2784 3485 513 Size: 552 957 526 236 701 3074 Fragments arranged by size: 3074 957 701 552 526 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4073 4090 4243 4428 4873 325 Size: 1255 2493 17 153 185 445 1498 Fragments arranged by size: 2493 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4292 6004 96 Size: 274 80 33 51 1443 2315 1712 138 Fragments arranged by size: 2315 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2976 3008 6006 6038 2976 Size: 32 2998 32 2984 Fragments arranged by size: 2998 2984 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 2821 3382 2821 Size: 561 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 2148 3371 1870 Size: 278 1223 4545 Fragments arranged by size: 4545 1223 278 XmnI GAAnn'nnTTC Cuts at: 2422 2706 2817 4175 2422 Size: 284 111 1358 4293 Fragments arranged by size: 4293 1358 284 111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI