(Circular) (Six-base) MAPSORT of: pFN21ASDF0041.seq Check: 7850 from: 1 to: 5662 pFN21ASDF0041 5662 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2189 2325 2512 2779 2189 Size: 136 187 267 5072 Fragments arranged by size: 5072 267 187 136 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2904 3472 278 Size: 53 83 186 936 1368 568 2468 Fragments arranged by size: 2468 1368 936 568 186 83 53 AccI GT'mk_AC Cuts at: 1937 2667 3005 1937 Size: 730 338 4594 Fragments arranged by size: 4594 730 338 Acc65I G'GTAC_C Cuts at: 2238 2983 2238 Size: 745 4917 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3725 5109 5377 1632 Size: 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5465 AflIII A'CryG_T Cuts at: 1203 4561 5538 1203 Size: 3358 977 1327 Fragments arranged by size: 3358 1327 977 AgeI A'CCGG_T Cuts at: 4493 4493 Size: 5662 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5662 AlwNI CAG_nnn'CTG Cuts at: 2710 3405 4977 2710 Size: 695 1572 3395 Fragments arranged by size: 3395 1572 695 ApaI G_GGCC'C Cuts at: 2198 2198 Size: 5662 ApaLI G'TGCA_C Cuts at: 3719 4875 3719 Size: 1156 4506 ApoI r'AATT_y Cuts at: 1493 2971 3197 3349 5458 1493 Size: 1478 226 152 2109 1697 Fragments arranged by size: 2109 1697 1478 226 152 AseI AT'TA_AT Cuts at: 160 1032 4216 160 Size: 872 3184 1606 Fragments arranged by size: 3184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2156 2248 2786 2987 1870 Size: 73 213 92 538 201 4545 Fragments arranged by size: 4545 538 213 201 92 73 AvrII C'CTAG_G Cuts at: 2101 2101 Size: 5662 BamHI G'GATC_C Cuts at: 2066 2992 3359 2066 Size: 926 367 4369 Fragments arranged by size: 4369 926 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 2478 2507 2618 2802 Size: 325 217 627 451 240 29 111 184 Cuts at: 2802 2983 4438 618 Size: 181 1455 1842 Fragments arranged by size: 1842 1455 627 451 325 240 217 184 181 111 29 BanII G_rGCy'C Cuts at: 1391 2198 2868 2981 1391 Size: 807 670 113 4072 Fragments arranged by size: 4072 807 670 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2896 928 Size: 971 997 3694 Fragments arranged by size: 3694 997 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3853 3887 1903 Size: 34 1916 34 3678 Fragments arranged by size: 3678 1916 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2775 3554 4764 2775 Size: 779 1210 3673 Fragments arranged by size: 3673 1210 779 BclI T'GATC_A Cuts at: 1548 1548 Size: 5662 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 4273 136 Size: 107 122 71 631 605 373 2228 1525 Fragments arranged by size: 2228 1525 631 605 373 122 107 71 BglII A'GATC_T Cuts at: 5657 5657 Size: 5662 BlpI GC'TnA_GC Cuts at: 2364 3079 2364 Size: 715 4947 Bme1580I G_kGCm'C Cuts at: 1790 2198 2214 2704 3723 4879 1790 Size: 408 16 490 1019 1156 2573 Fragments arranged by size: 2573 1156 1019 490 408 16 BmgBI CAC'GTC Cuts at: 1318 2235 1318 Size: 917 4745 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3393 4351 442 Size: 416 53 83 399 24 1976 958 1753 Fragments arranged by size: 1976 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5662 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2404 2404 Size: 5662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2399 2871 4322 1239 Size: 824 336 472 1451 2579 Fragments arranged by size: 2579 1451 824 472 336 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3787 4652 4950 3787 Size: 865 298 4499 Fragments arranged by size: 4499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4325 882 Size: 617 2826 2219 Fragments arranged by size: 2826 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2959 3453 493 Size: 711 1755 494 2702 Fragments arranged by size: 2702 1755 711 494 BsaBI GATnn'nnATC Cuts at: 5656 5656 Size: 5662 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 2901 3469 3851 Size: 53 83 186 936 495 873 568 382 Cuts at: 3851 4486 275 Size: 635 1451 Fragments arranged by size: 1451 936 873 635 568 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2107 4095 4493 4767 4914 5325 2107 Size: 1988 398 274 147 411 2444 Fragments arranged by size: 2444 1988 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 1178 Size: 30 165 30 1152 30 4255 Fragments arranged by size: 4255 1152 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2563 2874 1186 Size: 1377 311 3974 Fragments arranged by size: 3974 1377 311 BseYI C'CCAG_C Cuts at: 2061 2823 2867 4865 5361 2061 Size: 762 44 1998 496 2362 Fragments arranged by size: 2362 1998 762 496 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 2303 2546 1163 Size: 573 546 21 243 4279 Fragments arranged by size: 4279 573 546 243 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2496 3723 3808 4879 2496 Size: 1227 85 1071 3279 Fragments arranged by size: 3279 1227 1071 85 BsmI GAATG_Cn' Cuts at: 3173 3266 3173 Size: 93 5569 BsmBI CGTCTCn'nnnn_ Cuts at: 2892 2892 Size: 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2214 2496 2704 2868 2981 3723 Size: 399 408 16 282 208 164 113 742 Cuts at: 3723 3808 4879 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 282 208 164 113 85 16 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 3551 5281 3551 Size: 1730 3932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2189 2276 2325 2512 2779 3017 844 Size: 1345 87 49 187 267 238 3489 Fragments arranged by size: 3489 1345 267 238 187 87 49 BsrBI CCG'CTC Cuts at: 1394 1823 3549 1394 Size: 429 1726 3507 Fragments arranged by size: 3507 1726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4157 4331 65 Size: 1183 2909 174 1396 Fragments arranged by size: 2909 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2280 4306 4493 1700 Size: 110 238 232 2026 187 2869 Fragments arranged by size: 2869 2026 238 232 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 3722 4734 3722 Size: 1012 4650 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 2007 4466 2007 Size: 2459 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5662 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2557 2041 Size: 516 5146 BstYI r'GATC_y Cuts at: 1972 2066 2992 3359 3744 3761 5213 5299 5311 Size: 94 926 367 385 17 1452 86 12 Cuts at: 5311 5657 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 926 385 367 346 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2034 2323 2371 2398 2503 2511 Size: 552 957 12 289 48 27 105 8 Cuts at: 2511 513 Size: 3664 Fragments arranged by size: 3664 957 552 289 105 48 27 12 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4923 Fragments arranged by size: 4923 683 56 BtsI GCAGTG_nn' Cuts at: 3178 3972 3992 3178 Size: 794 20 4848 Fragments arranged by size: 4848 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5627 DraI TTT'AAA Cuts at: 2966 2966 Size: 5662 DraIII CAC_nnn'GTG Cuts at: 2218 2218 Size: 5662 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3109 3998 5627 Size: 54 1425 50 275 18 1279 889 1629 Cuts at: 5627 8 Size: 43 Fragments arranged by size: 1629 1425 1279 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2090 3592 1353 Size: 728 9 1502 3423 Fragments arranged by size: 3423 1502 728 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2399 2871 3725 4322 5109 5377 Size: 393 431 336 472 854 597 787 268 Cuts at: 5377 1239 Size: 1524 Fragments arranged by size: 1524 854 787 597 472 431 393 336 268 EcoO109I rG'GnC_Cy Cuts at: 2195 2308 2891 2195 Size: 113 583 4966 Fragments arranged by size: 4966 583 113 EcoRI G'AATT_C Cuts at: 2971 3349 2971 Size: 378 5284 FspI TGC'GCA Cuts at: 2423 4168 2423 Size: 1745 3917 HaeII r_GCGC'y Cuts at: 1678 2062 2281 2371 4809 1678 Size: 384 219 90 2438 2531 Fragments arranged by size: 2531 2438 384 219 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2531 2563 4303 Size: 32 135 32 486 32 441 32 1740 Cuts at: 4303 4335 4377 4409 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1740 486 441 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3006 3252 669 Size: 988 281 1068 246 3079 Fragments arranged by size: 3079 1068 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2242 2987 2242 Size: 745 4917 MfeI C'AATT_G Cuts at: 3261 3261 Size: 5662 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2415 2574 2715 4776 4960 1305 Size: 1110 159 141 2061 184 2007 Fragments arranged by size: 2061 2007 1110 184 159 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3840 Fragments arranged by size: 3840 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 3620 3979 4138 518 Size: 648 1251 1203 359 159 2042 Fragments arranged by size: 2042 1251 1203 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2827 3078 3755 4903 5148 1606 Size: 449 772 251 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 449 251 245 NaeI GCC'GGC Cuts at: 2050 2282 2050 Size: 232 5430 NcoI C'CATG_G Cuts at: 513 1065 2022 2323 2398 2503 2511 513 Size: 552 957 301 75 105 8 3664 Fragments arranged by size: 3664 957 552 301 105 75 8 NdeI CA'TA_TG Cuts at: 387 387 Size: 5662 NgoMIV G'CCGG_C Cuts at: 2048 2280 2048 Size: 232 5430 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5662 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 3020 4565 1132 Size: 97 1791 1545 2229 Fragments arranged by size: 2229 1791 1545 97 PasI CC'CwG_GG Cuts at: 1348 2776 1348 Size: 1428 4234 PciI A'CATG_T Cuts at: 4561 4561 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 1108 2359 2377 2710 1108 Size: 1251 18 333 4060 Fragments arranged by size: 4060 1251 333 18 PfoI T'CCnGG_A Cuts at: 2304 2304 Size: 5662 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3763 5244 5276 3731 Size: 32 1481 32 4117 Fragments arranged by size: 4117 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2308 Size: 5662 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5662 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PspOMI G'GGCC_C Cuts at: 2194 2194 Size: 5662 PspXI vC'TCGA_Gb Cuts at: 2248 2248 Size: 5662 PstI C_TGCA'G Cuts at: 830 2186 2523 2605 3014 830 Size: 1356 337 82 409 3478 Fragments arranged by size: 3478 1356 409 337 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 PvuII CAG'CTG Cuts at: 2055 2055 Size: 5662 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SapI GCTCTTCn'nnn_ Cuts at: 2081 2081 Size: 5662 SbfI CC_TGCA'GG Cuts at: 2186 3014 2186 Size: 828 4834 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SfcI C'TryA_G Cuts at: 826 1045 2182 2519 2601 3010 4145 4826 5017 Size: 219 1137 337 82 409 1135 681 191 Cuts at: 5017 826 Size: 1471 Fragments arranged by size: 1471 1137 1135 681 409 337 219 191 82 SfiI GGCCn_nnn'nGGCC Cuts at: 2045 2045 Size: 5662 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2989 1872 Size: 1117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2248 3766 4667 4929 820 Size: 197 926 305 1518 901 262 1553 Fragments arranged by size: 1553 1518 926 901 305 262 197 SnaBI TAC'GTA Cuts at: 493 2959 3453 493 Size: 2466 494 2702 Fragments arranged by size: 2702 2466 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 2022 2101 2323 2398 2503 2511 3101 Size: 552 957 79 222 75 105 8 590 Cuts at: 3101 513 Size: 3074 Fragments arranged by size: 3074 957 590 552 222 105 79 75 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3689 3706 3859 4044 4489 325 Size: 1255 2109 17 153 185 445 1498 Fragments arranged by size: 2109 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3908 5620 96 Size: 274 80 33 51 1443 1931 1712 138 Fragments arranged by size: 1931 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2795 2827 5622 5654 2795 Size: 32 2795 32 2803 Fragments arranged by size: 2803 2795 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2924 1316 Size: 1608 4054 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2356 Size: 5662 XhoI C'TCGA_G Cuts at: 1943 2248 1943 Size: 305 5357 XmaI C'CCGG_G Cuts at: 1870 2987 1870 Size: 1117 4545 XmnI GAAnn'nnTTC Cuts at: 3791 3791 Size: 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2902 3470 276 Size: 53 83 186 936 1368 568 2468 Fragments arranged by size: 2468 1368 936 568 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI