Restriction map for
pFN21AB9403
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# NO CUT ENZYMES ( 73 enzymes) #
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AccI AclI AflII AflIII AgeI
AhdI AscI AseI AsnI AspEI
BamHI BbrPI BciVI BclI BfrI
BsaBI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI Eam1105I EcoO65I EcoRV
EcoT22I FbaI FseI HindIII HpaI
MamI MfeI MluI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RsrII SalI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 2074 GACGTC GACGT^C
Alw44I 700 GTGCAC G^TGCAC
ApaLI 700 GTGCAC G^TGCAC
Asp700I 286 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1491 TGCGCA TGC^GCA
AvrII 3006 CCTAGG C^CTAGG
BbeI 202 GGCGCC GGCGC^C
BlnI 3006 CCTAGG C^CTAGG
BlpI 2284 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2284 GCTGAGC GC^T[ACGT]AGC
BspHI 930 TCATGA T^CATGA
BsrDI 3096 CATTGC GCAATG(2/0)
BsrFI 3332 GCCGGC [GA]^CCGG[CT]
CelII 2284 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3332 GCCGGC [GA]^CCGG[CT]
DraIII 1554 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2068 GACACTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3334 CGGCCG C^GGCCG
EclXI 3334 CGGCCG C^GGCCG
Eco52I 3334 CGGCCG C^GGCCG
FspI 1491 TGCGCA TGC^GCA
KasI 202 GGCGCC G^GCGCC
KspI 171 CCGCGG CCGC^GG
NaeI 3332 GCCGGC GCC^GGC
NarI 202 GGCGCC GG^CGCC
NcoI 1901 CCATGG C^CATGG
NgoMIV 3332 GCCGGC G^CCGGC
PvuI 856 CGATCG CGAT^CG
RcaI 930 TCATGA T^CATGA
SacI 2287 GAGCTC GAGCT^C
SacII 171 CCGCGG CCGC^GG
SbfI 2373 CCTGCAGG CCTGCA^GG
SfoI 202 GGCGCC GGC^GCC
SphI 1667 GCATGC GCATG^C
Sse8387I 2373 CCTGCAGG CCTGCA^GG
SspI 683 AATATT AAT^ATT
XmnI 286 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccIII 2514 TCCGGA T^CCGGA
3116 TCCGGA
AcsI 286 GAATTC [GA]^AATT[CT]
2489 GAATTC
AcyI 202 GGCGCC G[GA]^CG[CT]C
2074 GACGTC
AocI 661 CCTGAGG CC^T[ACGT]AGG
3674 CCTCAGG
ApaI 1319 GGGCCC GGGCC^C
3018 GGGCCC
ApoI 286 GAATTC [GA]^AATT[CT]
2489 GAATTC
BalI 981 TGGCCA TGG^CCA
1380 TGGCCA
BglII 2430 AGATCT A^GATCT
3348 AGATCT
BsaHI 202 GGCGCC G[GA]^CG[CT]C
2074 GACGTC
BseAI 2514 TCCGGA T^CCGGA
3116 TCCGGA
BsmI 1184 GAATGC GAATGC(1/-1)
241 GCATTC
BspEI 2514 TCCGGA T^CCGGA
3116 TCCGGA
Bsu36I 661 CCTGAGG CC^T[ACGT]AGG
3674 CCTCAGG
Eco81I 661 CCTGAGG CC^T[ACGT]AGG
3674 CCTCAGG
EcoNI 1720 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2022 CCTTCTCAAGG
EcoRI 286 GAATTC G^AATTC
2489 GAATTC
Hin1I 202 GGCGCC G[GA]^CG[CT]C
2074 GACGTC
HincII 136 GTCAAC GT[CT]^[GA]AC
997 GTCAAC
HindII 136 GTCAAC GT[CT]^[GA]AC
997 GTCAAC
MluNI 981 TGGCCA TGG^CCA
1380 TGGCCA
MroI 2514 TCCGGA T^CCGGA
3116 TCCGGA
MscI 981 TGGCCA TGG^CCA
1380 TGGCCA
MseI 692 TTAA T^TAA
2144 TTAA
NdeI 316 CATATG CA^TATG
1199 CATATG
NspI 1667 GCATGC [GA]CATG^[CT]
3653 GCATGT
PflMI 2115 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2220 CCAGCACTTGG
SfiI 1138 GGCCCAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3759 GGCCTCCGAGGCC
StuI 2460 AGGCCT AGG^CCT
3767 AGGCCT
Tru9I 692 TTAA T^TAA
2144 TTAA
Van91I 2115 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2220 CCAGCACTTGG
XcmI 482 CCAATGTTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1892 CCATGCCCTCCATGG
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI Aor51HI Asp718I AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII