(Circular) (Six-base) MAPSORT of: pFN21AB9403.seq Check: 3603 from: 1 to: 8545 pFN21AB9403 8545 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5800 5800 Size: 8545 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4101 6355 278 Size: 53 83 186 936 2565 2254 2468 Fragments arranged by size: 2565 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5888 1937 Size: 3951 4594 Acc65I G'GTAC_C Cuts at: 3255 5055 5631 5866 3255 Size: 1800 576 235 5934 Fragments arranged by size: 5934 1800 576 235 AclI AA'CG_TT Cuts at: 1563 6672 7045 1563 Size: 5109 373 3063 Fragments arranged by size: 5109 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2791 2909 3557 4019 4085 4379 4406 4492 Size: 1159 118 648 462 66 294 27 86 Cuts at: 4492 4721 6608 7992 8260 1632 Size: 229 1887 1384 268 1917 Fragments arranged by size: 1917 1887 1384 1159 648 462 294 268 229 118 86 66 27 AfeI AGC'GCT Cuts at: 2742 3952 5576 2742 Size: 1210 1624 5711 Fragments arranged by size: 5711 1624 1210 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8348 AflIII A'CryG_T Cuts at: 1203 7444 8421 1203 Size: 6241 977 1327 Fragments arranged by size: 6241 1327 977 AgeI A'CCGG_T Cuts at: 7376 7376 Size: 8545 AhdI GACnn_n'nnGTC Cuts at: 7274 7274 Size: 8545 AleI CACnn'nnGTG Cuts at: 1166 4880 1166 Size: 3714 4831 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2669 3707 1184 Size: 1485 1038 6022 Fragments arranged by size: 6022 1485 1038 AlwNI CAG_nnn'CTG Cuts at: 2872 4396 5065 5416 5831 6288 7860 2872 Size: 1524 669 351 415 457 1572 3557 Fragments arranged by size: 3557 1572 1524 669 457 415 351 ApaI G_GGCC'C Cuts at: 3346 5045 3346 Size: 1699 6846 ApaLI G'TGCA_C Cuts at: 2723 6602 7758 2723 Size: 3879 1156 3510 Fragments arranged by size: 3879 3510 1156 ApoI r'AATT_y Cuts at: 1493 2309 4512 5854 6080 6232 8341 1493 Size: 816 2203 1342 226 152 2109 1697 Fragments arranged by size: 2203 2109 1697 1342 816 226 152 AseI AT'TA_AT Cuts at: 160 1032 7099 160 Size: 872 6067 1606 Fragments arranged by size: 6067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8545 AvaI C'yCGr_G Cuts at: 1870 1943 2164 2639 3463 5870 1870 Size: 73 221 475 824 2407 4545 Fragments arranged by size: 4545 2407 824 475 221 73 AvrII C'CTAG_G Cuts at: 5029 5029 Size: 8545 BamHI G'GATC_C Cuts at: 5875 6242 5875 Size: 367 8178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2225 3255 3575 3700 4552 Size: 325 217 627 438 1030 320 125 852 Cuts at: 4552 5055 5631 5866 7321 618 Size: 503 576 235 1455 1842 Fragments arranged by size: 1842 1455 1030 852 627 576 503 438 325 320 235 217 125 BanII G_rGCy'C Cuts at: 1391 2157 2771 3346 3473 4165 4314 5045 5082 Size: 766 614 575 127 692 149 731 37 Cuts at: 5082 5864 1391 Size: 782 4072 Fragments arranged by size: 4072 782 766 731 692 614 575 149 127 37 BbeI G_GCGC'C Cuts at: 2229 2229 Size: 8545 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 3359 3757 4155 928 Size: 971 190 1270 398 398 5318 Fragments arranged by size: 5318 1270 971 398 398 190 BbvCI CC'TCA_GC Cuts at: 4715 5003 5351 4715 Size: 288 348 7909 Fragments arranged by size: 7909 348 288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2116 2150 3116 3150 5644 5678 6736 Size: 34 179 34 966 34 2494 34 1058 Cuts at: 6736 6770 1903 Size: 34 3678 Fragments arranged by size: 3678 2494 1058 966 179 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6437 7647 6437 Size: 1210 7335 BclI T'GATC_A Cuts at: 1548 1548 Size: 8545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2088 3168 5789 Size: 107 122 71 631 605 416 1080 2621 Cuts at: 5789 7156 136 Size: 1367 1525 Fragments arranged by size: 2621 1525 1367 1080 631 605 416 122 107 71 BglII A'GATC_T Cuts at: 4453 5371 8540 4453 Size: 918 3169 4458 Fragments arranged by size: 4458 3169 918 BlpI GC'TnA_GC Cuts at: 4308 5962 4308 Size: 1654 6891 Bme1580I G_kGCm'C Cuts at: 1790 2727 3346 3705 3871 5045 6606 7762 1790 Size: 937 619 359 166 1174 1561 1156 2573 Fragments arranged by size: 2573 1561 1174 1156 937 619 359 166 BmgBI CAC'GTC Cuts at: 1318 5094 1318 Size: 3776 4769 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3343 3951 4614 Size: 416 53 83 399 24 1926 608 663 Cuts at: 4614 5318 6276 7234 442 Size: 704 958 958 1753 Fragments arranged by size: 1926 1753 958 958 704 663 608 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8545 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4328 4328 Size: 8545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2280 2438 3098 3977 4031 4121 4220 4300 Size: 1041 158 660 879 54 90 99 80 Cuts at: 4300 4673 5087 5276 5388 5525 7205 1239 Size: 373 414 189 112 137 1680 2579 Fragments arranged by size: 2579 1680 1041 879 660 414 373 189 158 137 112 99 90 80 54 Bpu10I CC'TnA_GC Cuts at: 2058 4715 4989 5003 5351 5571 2058 Size: 2657 274 14 348 220 5032 Fragments arranged by size: 5032 2657 348 274 220 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4034 4877 5496 5633 6670 7535 7833 4034 Size: 843 619 137 1037 865 298 4746 Fragments arranged by size: 4746 1037 865 843 619 298 137 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2475 3114 4227 4914 5153 7208 882 Size: 617 976 639 1113 687 239 2055 2219 Fragments arranged by size: 2219 2055 1113 976 687 639 617 239 BsaAI yAC'GTr Cuts at: 493 1204 2665 4687 5581 5842 6336 493 Size: 711 1461 2022 894 261 494 2702 Fragments arranged by size: 2702 2022 1461 894 711 494 261 BsaBI GATnn'nnATC Cuts at: 8539 8539 Size: 8545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2226 4098 6352 6734 Size: 53 83 186 936 693 1872 2254 382 Cuts at: 6734 7369 275 Size: 635 1451 Fragments arranged by size: 2254 1872 1451 936 693 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3093 3817 4537 5139 6978 7376 7650 7797 8208 Size: 724 720 602 1839 398 274 147 411 Cuts at: 8208 3093 Size: 3430 Fragments arranged by size: 3430 1839 724 720 602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3896 3926 4955 4985 5107 Size: 30 165 30 2493 30 1029 30 122 Cuts at: 5107 5137 5192 5222 1178 Size: 30 55 30 4501 Fragments arranged by size: 4501 2493 1029 165 122 55 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2234 3143 3463 3841 3844 5261 5468 Size: 1033 15 909 320 378 3 1417 207 Cuts at: 5468 5630 1186 Size: 162 4101 Fragments arranged by size: 4101 1417 1033 909 378 320 207 162 15 3 BseYI C'CCAG_C Cuts at: 2199 2399 2657 3847 4242 4671 4710 4930 5019 Size: 200 258 1190 395 429 39 220 89 Cuts at: 5019 5606 5796 7748 8244 2199 Size: 587 190 1952 496 2500 Fragments arranged by size: 2500 1952 1190 587 496 429 395 258 220 200 190 89 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2107 2519 2847 5262 5495 1163 Size: 573 371 412 328 2415 233 4213 Fragments arranged by size: 4213 2415 573 412 371 328 233 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2882 4973 5360 5995 6756 Size: 275 186 39 842 2091 387 635 761 Cuts at: 6756 7784 1540 Size: 1028 2301 Fragments arranged by size: 2301 2091 1028 842 761 635 387 275 186 39 BsiHKAI G_wGCw'C Cuts at: 2727 3333 3452 4314 6606 6691 7762 2727 Size: 606 119 862 2292 85 1071 3510 Fragments arranged by size: 3510 2292 1071 862 606 119 85 BsiWI C'GTAC_G Cuts at: 5838 5838 Size: 8545 BsmI GAATG_Cn' Cuts at: 2264 3213 6056 6149 2264 Size: 949 2843 93 4660 Fragments arranged by size: 4660 2843 949 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2727 2771 3333 3346 3452 3473 Size: 399 367 570 44 562 13 106 21 Cuts at: 3473 3705 3871 4165 4314 5045 5082 5864 6606 Size: 232 166 294 149 731 37 782 742 Cuts at: 6606 6691 7762 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 782 742 731 570 562 399 367 294 232 166 149 106 85 44 37 21 13 BspEI T'CCGG_A Cuts at: 4537 5139 8208 4537 Size: 602 3069 4874 Fragments arranged by size: 4874 3069 602 BspHI T'CATG_A Cuts at: 2953 6434 8164 2953 Size: 3481 1730 3334 Fragments arranged by size: 3481 3334 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 3501 5043 5415 5800 5900 844 Size: 1352 1305 1542 372 385 100 3489 Fragments arranged by size: 3489 1542 1352 1305 385 372 100 BsrBI CCG'CTC Cuts at: 1394 1823 2181 2914 3708 5362 6432 1394 Size: 429 358 733 794 1654 1070 3507 Fragments arranged by size: 3507 1654 1070 794 733 429 358 BsrDI GCAATG_nn' Cuts at: 65 1248 5118 7040 7214 65 Size: 1183 3870 1922 174 1396 Fragments arranged by size: 3870 1922 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5355 7189 7376 1700 Size: 110 3545 1834 187 2869 Fragments arranged by size: 3545 2869 1834 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8545 BssSI C'ACGA_G Cuts at: 6605 7617 6605 Size: 1012 7533 BstAPI GCAn_nnn'nTGC Cuts at: 794 4276 4396 5482 794 Size: 3482 120 1086 3857 Fragments arranged by size: 3857 3482 1086 120 BstBI TT'CG_AA Cuts at: 2007 7349 2007 Size: 5342 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8545 BstXI CCAn_nnnn'nTGG Cuts at: 2471 3401 4333 2471 Size: 930 932 6683 Fragments arranged by size: 6683 932 930 BstYI r'GATC_y Cuts at: 1972 4021 4453 5371 5590 5649 5875 6242 6627 Size: 2049 432 918 219 59 226 367 385 Cuts at: 6627 6644 8096 8182 8194 8540 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2049 1977 1452 918 432 385 367 346 226 219 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2685 5698 2685 Size: 3013 5532 BtgI C'CryG_G Cuts at: 513 1065 2022 2194 2394 2514 3924 513 Size: 552 957 172 200 120 1410 5134 Fragments arranged by size: 5134 1410 957 552 200 172 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7806 Fragments arranged by size: 7806 683 56 BtsI GCAGTG_nn' Cuts at: 4476 4943 5832 6061 6855 6875 4476 Size: 467 889 229 794 20 6146 Fragments arranged by size: 6146 889 794 467 229 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8510 DraI TTT'AAA Cuts at: 5849 5849 Size: 8545 DraIII CAC_nnn'GTG Cuts at: 3582 3582 Size: 8545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4097 7552 809 Size: 507 2781 3455 1802 Fragments arranged by size: 3455 2781 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2962 3004 Size: 54 1425 50 275 18 259 873 42 Cuts at: 3004 3403 3779 5357 5992 6881 8510 8 Size: 399 376 1578 635 889 1629 43 Fragments arranged by size: 1629 1578 1425 889 873 635 399 376 275 259 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 5357 5992 1537 Size: 275 3545 635 4090 Fragments arranged by size: 4090 3545 635 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2471 2885 3444 3546 4408 4670 5592 Size: 966 152 414 559 102 862 262 922 Cuts at: 5592 6475 1353 Size: 883 3423 Fragments arranged by size: 3423 966 922 883 862 559 414 262 152 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7132 7506 7652 387 Size: 1064 138 5543 374 146 1280 Fragments arranged by size: 5543 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4312 4312 Size: 8545 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2280 2438 2791 2909 3098 3557 3977 Size: 393 648 158 353 118 189 459 420 Cuts at: 3977 4019 4031 4085 4121 4220 4300 4379 4406 Size: 42 12 54 36 99 80 79 27 Cuts at: 4406 4492 4673 4721 5087 5276 5388 5525 6608 Size: 86 181 48 366 189 112 137 1083 Cuts at: 6608 7205 7992 8260 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1083 787 648 597 459 420 393 366 353 268 189 189 181 158 137 118 112 99 86 80 79 54 48 42 36 27 12 EcoNI CCTnn'n_nnAGG Cuts at: 3747 4049 3747 Size: 302 8243 EcoO109I rG'GnC_Cy Cuts at: 2796 2972 3070 3342 3398 3752 4042 4132 4288 Size: 176 98 272 56 354 290 90 156 Cuts at: 4288 4791 4888 5042 5281 2796 Size: 503 97 154 239 6060 Fragments arranged by size: 6060 503 354 290 272 239 176 156 154 98 97 90 56 EcoRI G'AATT_C Cuts at: 2309 4512 5854 6232 2309 Size: 2203 1342 378 4622 Fragments arranged by size: 4622 2203 1342 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5191 5212 5191 Size: 21 8524 FspI TGC'GCA Cuts at: 3516 7051 3516 Size: 3535 5010 HaeII r_GCGC'y Cuts at: 1678 2229 2744 3559 3610 3641 3954 5206 5578 Size: 551 515 815 51 31 313 1252 372 Cuts at: 5578 7692 1678 Size: 2114 2531 Fragments arranged by size: 2531 2114 1252 815 551 515 372 313 51 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2424 2456 2730 Size: 32 135 32 479 32 341 32 274 Cuts at: 2730 2762 2977 3009 3373 3405 3896 3928 4955 Size: 32 215 32 364 32 491 32 1027 Cuts at: 4955 4987 5363 5395 5546 5578 7186 7218 7260 Size: 32 376 32 151 32 1608 32 42 Cuts at: 7260 7292 1373 Size: 32 2626 Fragments arranged by size: 2626 1608 1027 491 479 376 364 341 274 215 151 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 3022 5889 6135 669 Size: 988 281 223 861 2867 246 3079 Fragments arranged by size: 3079 2867 988 861 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 8545 HpaI GTT'AAC Cuts at: 6135 6135 Size: 8545 KasI G'GCGC_C Cuts at: 2225 2225 Size: 8545 KpnI G_GTAC'C Cuts at: 3259 5059 5635 5870 3259 Size: 1800 576 235 5934 Fragments arranged by size: 5934 1800 576 235 MfeI C'AATT_G Cuts at: 6144 6144 Size: 8545 MluI A'CGCG_T Cuts at: 8421 8421 Size: 8545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4344 5478 7659 7843 1305 Size: 3039 1134 2181 184 2007 Fragments arranged by size: 3039 2181 2007 1134 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3006 3405 10 Size: 54 1425 343 1174 399 5150 Fragments arranged by size: 5150 1425 1174 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 2958 3570 3716 4331 4880 6503 Size: 648 1566 226 612 146 615 549 1623 Cuts at: 6503 6862 7021 518 Size: 359 159 2042 Fragments arranged by size: 2042 1623 1566 648 615 612 549 359 226 159 146 MspA1I CmG'CkG Cuts at: 1606 2121 2196 2765 3262 3997 4384 4675 4714 Size: 515 75 569 497 735 387 291 39 Cuts at: 4714 5007 5254 5740 5961 6638 7786 8031 1606 Size: 293 247 486 221 677 1148 245 2120 Fragments arranged by size: 2120 1148 735 677 569 515 497 486 387 293 291 247 245 221 75 39 NaeI GCC'GGC Cuts at: 5357 5357 Size: 8545 NarI GG'CG_CC Cuts at: 2226 2226 Size: 8545 NcoI C'CATG_G Cuts at: 513 1065 2022 3924 513 Size: 552 957 1902 5134 Fragments arranged by size: 5134 1902 957 552 NdeI CA'TA_TG Cuts at: 387 2340 3223 387 Size: 1953 883 5709 Fragments arranged by size: 5709 1953 883 NgoMIV G'CCGG_C Cuts at: 5355 5355 Size: 8545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8545 NotI GC'GGCC_GC Cuts at: 5992 5992 Size: 8545 NspI r_CATG'y Cuts at: 1132 1229 3694 5680 5903 7448 1132 Size: 97 2465 1986 223 1545 2229 Fragments arranged by size: 2465 2229 1986 1545 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8545 PciI A'CATG_T Cuts at: 7444 7444 Size: 8545 PflMI CCAn_nnn'nTGG Cuts at: 1108 4144 4249 1108 Size: 3036 105 5404 Fragments arranged by size: 5404 3036 105 PfoI T'CCnGG_A Cuts at: 4137 4428 4137 Size: 291 8254 PmeI GTTT'AAAC Cuts at: 5849 5849 Size: 8545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6614 6646 8127 8159 6614 Size: 32 1481 32 7000 Fragments arranged by size: 7000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2796 2972 3070 3398 3752 4042 4132 4288 2796 Size: 176 98 328 354 290 90 156 7053 Fragments arranged by size: 7053 354 328 290 176 156 98 90 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8545 PsiI TTA'TAA Cuts at: 6115 6115 Size: 8545 PspOMI G'GGCC_C Cuts at: 3342 5041 3342 Size: 1699 6846 PstI C_TGCA'G Cuts at: 830 4284 4401 4483 5412 5625 5745 5897 830 Size: 3454 117 82 929 213 120 152 3478 Fragments arranged by size: 3478 3454 929 213 152 120 117 82 PvuI CG_AT'CG Cuts at: 2001 2882 2001 Size: 881 7664 PvuII CAG'CTG Cuts at: 3997 4384 4675 4714 5254 5740 3997 Size: 387 291 39 540 486 6802 Fragments arranged by size: 6802 540 486 387 291 39 SacI G_AGCT'C Cuts at: 4314 4314 Size: 8545 SacII CC_GC'GG Cuts at: 2197 2197 Size: 8545 SalI G'TCGA_C Cuts at: 1936 5887 1936 Size: 3951 4594 SanDI GG'GwC_CC Cuts at: 2972 3070 2972 Size: 98 8447 SapI GCTCTTCn'nnn_ Cuts at: 2319 3546 4408 5592 2319 Size: 1227 862 1184 5272 Fragments arranged by size: 5272 1227 1184 862 SbfI CC_TGCA'GG Cuts at: 4401 5897 4401 Size: 1496 7049 ScaI AGT'ACT Cuts at: 6793 8505 6793 Size: 1712 6833 SfcI C'TryA_G Cuts at: 826 1045 4280 4397 4479 4810 5408 5621 5687 Size: 219 3235 117 82 331 598 213 66 Cuts at: 5687 5741 5893 7028 7709 7900 826 Size: 54 152 1135 681 191 1471 Fragments arranged by size: 3235 1471 1135 681 598 331 219 213 191 152 117 82 66 54 SfiI GGCCn_nnn'nGGCC Cuts at: 3168 5789 3168 Size: 2621 5924 SfoI GGC'GCC Cuts at: 2227 2227 Size: 8545 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8545 SmaI CCC'GGG Cuts at: 1872 5872 1872 Size: 4000 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4049 4892 5511 5612 6649 7550 Size: 197 926 2106 843 619 101 1037 901 Cuts at: 7550 7812 820 Size: 262 1553 Fragments arranged by size: 2106 1553 1037 926 901 843 619 262 197 101 SnaBI TAC'GTA Cuts at: 493 5842 6336 493 Size: 5349 494 2702 Fragments arranged by size: 5349 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8545 SphI G_CATG'C Cuts at: 1132 3694 5903 1132 Size: 2562 2209 3774 Fragments arranged by size: 3774 2562 2209 SspI AAT'ATT Cuts at: 5 52 2708 6469 5 Size: 47 2656 3761 2081 Fragments arranged by size: 3761 2656 2081 47 StuI AGG'CCT Cuts at: 1517 4485 5792 1517 Size: 2968 1307 4270 Fragments arranged by size: 4270 2968 1307 StyI C'CwwG_G Cuts at: 513 1065 2022 3372 3649 3758 3860 3924 5029 Size: 552 957 1350 277 109 102 64 1105 Cuts at: 5029 5196 5314 5984 513 Size: 167 118 670 3074 Fragments arranged by size: 3074 1350 1105 957 670 552 277 167 118 109 102 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2834 3537 3921 4719 5620 6572 6589 Size: 1255 1254 703 384 798 901 952 17 Cuts at: 6589 6742 6927 7372 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1254 952 901 798 703 445 384 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5208 6791 8503 Size: 274 80 33 51 1443 3231 1583 1712 Cuts at: 8503 96 Size: 138 Fragments arranged by size: 3231 1712 1583 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2930 2962 5107 5139 8505 8537 2930 Size: 32 2145 32 3366 32 2938 Fragments arranged by size: 3366 2938 2145 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3017 4130 4292 1316 Size: 1701 1113 162 5569 Fragments arranged by size: 5569 1701 1113 162 XbaI T'CTAG_A Cuts at: 5881 5881 Size: 8545 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2512 3922 2512 Size: 1410 7135 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8545 XmaI C'CCGG_G Cuts at: 1870 5870 1870 Size: 4000 4545 XmnI GAAnn'nnTTC Cuts at: 2313 6674 2313 Size: 4361 4184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4099 6353 276 Size: 53 83 186 936 2565 2254 2468 Fragments arranged by size: 2565 2468 2254 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SrfI SwaI