(Circular) (Six-base) MAPSORT of: pFN21AB5641.seq Check: 2362 from: 1 to: 7006 pFN21AB5641 7006 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2100 4023 4816 278 Size: 53 83 186 936 564 1923 793 2468 Fragments arranged by size: 2468 1923 936 793 564 186 83 53 AccI GT'mk_AC Cuts at: 1937 3825 4349 1937 Size: 1888 524 4594 Fragments arranged by size: 4594 1888 524 Acc65I G'GTAC_C Cuts at: 4327 4327 Size: 7006 AclI AA'CG_TT Cuts at: 1563 2813 5133 5506 1563 Size: 1250 2320 373 3063 Fragments arranged by size: 3063 2320 1250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 5069 6453 6721 1632 Size: 1065 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 1065 268 AfeI AGC'GCT Cuts at: 3696 4061 3696 Size: 365 6641 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6809 AflIII A'CryG_T Cuts at: 1203 3432 4104 5905 6882 1203 Size: 2229 672 1801 977 1327 Fragments arranged by size: 2229 1801 1327 977 672 AgeI A'CCGG_T Cuts at: 5837 5837 Size: 7006 AhdI GACnn_n'nnGTC Cuts at: 3901 5735 3901 Size: 1834 5172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3509 1184 Size: 2325 4681 AlwNI CAG_nnn'CTG Cuts at: 2287 2779 3146 4749 6321 2287 Size: 492 367 1603 1572 2972 Fragments arranged by size: 2972 1603 1572 492 367 ApaLI G'TGCA_C Cuts at: 2757 5063 6219 2757 Size: 2306 1156 3544 Fragments arranged by size: 3544 2306 1156 ApoI r'AATT_y Cuts at: 1493 3588 4315 4541 4693 6802 1493 Size: 2095 727 226 152 2109 1697 Fragments arranged by size: 2109 2095 1697 727 226 152 AseI AT'TA_AT Cuts at: 160 1032 3111 5560 160 Size: 872 2079 2449 1606 Fragments arranged by size: 2449 2079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 AvaI C'yCGr_G Cuts at: 1870 1943 2458 4331 1870 Size: 73 515 1873 4545 Fragments arranged by size: 4545 1873 515 73 BamHI G'GATC_C Cuts at: 2269 2769 4336 4703 2269 Size: 500 1567 367 4572 Fragments arranged by size: 4572 1567 500 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 4327 5782 618 Size: 325 217 627 995 1545 1455 1842 Fragments arranged by size: 1842 1545 1455 995 627 325 217 BanII G_rGCy'C Cuts at: 1391 2393 2442 4325 1391 Size: 1002 49 1883 4072 Fragments arranged by size: 4072 1883 1002 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2685 928 Size: 971 786 5249 Fragments arranged by size: 5249 971 786 BbvCI CC'TCA_GC Cuts at: 2533 2533 Size: 7006 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4076 4110 5197 5231 1903 Size: 34 2139 34 1087 34 3678 Fragments arranged by size: 3678 2139 1087 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4898 6108 4898 Size: 1210 5796 BclI T'GATC_A Cuts at: 1548 2872 1548 Size: 1324 5682 BfrBI ATG'CAT Cuts at: 3200 3200 Size: 7006 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5617 136 Size: 107 122 71 631 605 3945 1525 Fragments arranged by size: 3945 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2521 3001 3654 7001 2521 Size: 480 653 3347 2526 Fragments arranged by size: 3347 2526 653 480 BlpI GC'TnA_GC Cuts at: 3461 4423 3461 Size: 962 6044 Bme1580I G_kGCm'C Cuts at: 1790 2761 5067 6223 1790 Size: 971 2306 1156 2573 Fragments arranged by size: 2573 2306 1156 971 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7006 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2659 3156 4737 Size: 416 53 83 399 24 1242 497 1581 Cuts at: 4737 5695 442 Size: 958 1753 Fragments arranged by size: 1753 1581 1242 958 497 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7006 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3676 3676 Size: 7006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2990 3467 5666 1239 Size: 1751 477 2199 2579 Fragments arranged by size: 2579 2199 1751 477 Bpu10I CC'TnA_GC Cuts at: 2387 2533 2387 Size: 146 6860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5131 5996 6294 5131 Size: 865 298 5843 Fragments arranged by size: 5843 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5669 882 Size: 617 4170 2219 Fragments arranged by size: 4170 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3799 4303 4797 493 Size: 711 2595 504 494 2702 Fragments arranged by size: 2702 2595 711 504 494 BsaBI GATnn'nnATC Cuts at: 7000 7000 Size: 7006 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 4020 4813 5195 Size: 53 83 186 936 564 1923 793 382 Cuts at: 5195 5830 275 Size: 635 1451 Fragments arranged by size: 1923 1451 936 793 635 564 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 5439 5837 6111 6258 6669 2766 Size: 2673 398 274 147 411 3103 Fragments arranged by size: 3103 2673 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3031 3061 1178 Size: 30 165 30 1628 30 5123 Fragments arranged by size: 5123 1628 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2810 1186 Size: 1624 5382 BseYI C'CCAG_C Cuts at: 2116 2290 2481 2592 2837 2995 6209 6705 2116 Size: 174 191 111 245 158 3214 496 2417 Fragments arranged by size: 3214 2417 496 245 191 174 158 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2262 2747 3479 3821 4139 1163 Size: 573 526 485 732 342 318 4030 Fragments arranged by size: 4030 732 573 526 485 342 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4456 5217 6245 1540 Size: 275 186 2455 761 1028 2301 Fragments arranged by size: 2455 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2761 5067 5152 6223 2761 Size: 2306 85 1071 3544 Fragments arranged by size: 3544 2306 1071 85 BsmI GAATG_Cn' Cuts at: 3691 4517 4610 3691 Size: 826 93 6087 Fragments arranged by size: 6087 826 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2992 2992 Size: 7006 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2393 2442 2761 4325 5067 5152 6223 Size: 399 603 49 319 1564 742 85 1071 Cuts at: 6223 1391 Size: 2174 Fragments arranged by size: 2174 1564 1071 742 603 399 319 85 49 BspEI T'CCGG_A Cuts at: 6669 6669 Size: 7006 BspHI T'CATG_A Cuts at: 4895 6625 4895 Size: 1730 5276 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4361 844 Size: 3517 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4893 1394 Size: 429 3070 3507 Fragments arranged by size: 3507 3070 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2928 3633 5501 5675 65 Size: 1183 1680 705 1868 174 1396 Fragments arranged by size: 1868 1680 1396 1183 705 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5650 5837 1700 Size: 110 3840 187 2869 Fragments arranged by size: 3840 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7006 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7006 BssSI C'ACGA_G Cuts at: 3650 5066 6078 3650 Size: 1416 1012 4578 Fragments arranged by size: 4578 1416 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7006 BstBI TT'CG_AA Cuts at: 2007 5810 2007 Size: 3803 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7006 BstXI CCAn_nnnn'nTGG Cuts at: 3670 3697 3670 Size: 27 6979 BstYI r'GATC_y Cuts at: 1972 2269 2521 2769 3001 3654 4336 4703 5088 Size: 297 252 248 232 653 682 367 385 Cuts at: 5088 5105 6557 6643 6655 7001 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 682 653 385 367 346 297 252 248 232 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2256 2569 3074 3477 513 Size: 552 1191 313 505 403 4042 Fragments arranged by size: 4042 1191 552 505 403 313 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2805 487 Size: 683 56 1579 4688 Fragments arranged by size: 4688 1579 683 56 BtsI GCAGTG_nn' Cuts at: 2073 2394 2759 4522 5316 5336 2073 Size: 321 365 1763 794 20 3743 Fragments arranged by size: 3743 1763 794 365 321 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7006 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2605 2640 529 Size: 35 2041 35 4895 Fragments arranged by size: 4895 2041 35 35 DraI TTT'AAA Cuts at: 3168 4310 3168 Size: 1142 5864 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2815 3833 6013 809 Size: 507 1499 1018 2180 1802 Fragments arranged by size: 2180 1802 1499 1018 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2253 2683 Size: 54 1425 50 275 18 246 177 430 Cuts at: 2683 3547 4453 5342 6971 8 Size: 864 906 889 1629 43 Fragments arranged by size: 1629 1425 906 889 864 430 275 246 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4453 1537 Size: 275 2641 4090 Fragments arranged by size: 4090 2641 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 3312 3954 4213 4936 1353 Size: 1224 735 642 259 723 3423 Fragments arranged by size: 3423 1224 735 723 642 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5593 5967 6113 387 Size: 1064 138 4004 374 146 1280 Fragments arranged by size: 4004 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2697 2990 3467 5069 5666 6453 6721 Size: 393 1065 293 477 1602 597 787 268 Cuts at: 6721 1239 Size: 1524 Fragments arranged by size: 1602 1524 1065 787 597 477 393 293 268 EcoO109I rG'GnC_Cy Cuts at: 2371 2937 3322 2371 Size: 566 385 6055 Fragments arranged by size: 6055 566 385 EcoRI G'AATT_C Cuts at: 4315 4693 4315 Size: 378 6628 EcoRV GAT'ATC Cuts at: 4142 4142 Size: 7006 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3044 3044 Size: 7006 FspI TGC'GCA Cuts at: 5512 5512 Size: 7006 HaeII r_GCGC'y Cuts at: 1678 3698 4063 6153 1678 Size: 2020 365 2090 2531 Fragments arranged by size: 2531 2090 2020 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2783 2815 2816 2848 3390 Size: 32 135 32 1211 32 1 32 542 Cuts at: 3390 3422 3887 3919 5647 5679 5721 5753 1373 Size: 32 465 32 1728 32 42 32 2626 Fragments arranged by size: 2626 1728 1211 542 465 135 42 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2056 3151 3355 4350 4596 669 Size: 988 281 118 1095 204 995 246 3079 Fragments arranged by size: 3079 1095 995 988 281 246 204 118 HindIII A'AGCT_T Cuts at: 748 748 Size: 7006 HpaI GTT'AAC Cuts at: 3151 4596 3151 Size: 1445 5561 KpnI G_GTAC'C Cuts at: 4331 4331 Size: 7006 MfeI C'AATT_G Cuts at: 4605 4605 Size: 7006 MluI A'CGCG_T Cuts at: 6882 6882 Size: 7006 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3788 3817 3909 6120 6304 1305 Size: 2483 29 92 2211 184 2007 Fragments arranged by size: 2483 2211 2007 184 92 29 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 10 Size: 54 1425 343 246 4938 Fragments arranged by size: 4938 1425 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 3231 3521 3917 4292 4964 5323 5482 Size: 648 2065 290 396 375 672 359 159 Cuts at: 5482 518 Size: 2042 Fragments arranged by size: 2065 2042 672 648 396 375 359 290 159 MspA1I CmG'CkG Cuts at: 1606 2042 2085 2276 2805 4422 5099 6247 6492 Size: 436 43 191 529 1617 677 1148 245 Cuts at: 6492 1606 Size: 2120 Fragments arranged by size: 2120 1617 1148 677 529 436 245 191 43 NcoI C'CATG_G Cuts at: 513 1065 2256 2569 3074 3477 513 Size: 552 1191 313 505 403 4042 Fragments arranged by size: 4042 1191 552 505 403 313 NdeI CA'TA_TG Cuts at: 387 387 Size: 7006 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7006 NotI GC'GGCC_GC Cuts at: 4453 4453 Size: 7006 NsiI A_TGCA'T Cuts at: 3202 3202 Size: 7006 NspI r_CATG'y Cuts at: 1132 1229 3140 3436 3460 3817 4135 4364 5909 Size: 97 1911 296 24 357 318 229 1545 Cuts at: 5909 1132 Size: 2229 Fragments arranged by size: 2229 1911 1545 357 318 296 229 97 24 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7006 PciI A'CATG_T Cuts at: 3432 5905 3432 Size: 2473 4533 PflMI CCAn_nnn'nTGG Cuts at: 1108 2779 1108 Size: 1671 5335 PfoI T'CCnGG_A Cuts at: 3444 3444 Size: 7006 PmeI GTTT'AAAC Cuts at: 4310 4310 Size: 7006 PmlI CAC'GTG Cuts at: 3799 3799 Size: 7006 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5075 5107 6588 6620 5075 Size: 32 1481 32 5461 Fragments arranged by size: 5461 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 2937 3322 2371 Size: 566 385 6055 Fragments arranged by size: 6055 566 385 PshAI GACnn'nnGTC Cuts at: 1613 2665 1613 Size: 1052 5954 PsiI TTA'TAA Cuts at: 4576 4576 Size: 7006 PspXI vC'TCGA_Gb Cuts at: 2458 2458 Size: 7006 PstI C_TGCA'G Cuts at: 830 2356 2401 2649 3707 4358 830 Size: 1526 45 248 1058 651 3478 Fragments arranged by size: 3478 1526 1058 651 248 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7006 PvuII CAG'CTG Cuts at: 2042 2276 2805 2042 Size: 234 529 6243 Fragments arranged by size: 6243 529 234 SalI G'TCGA_C Cuts at: 1936 4348 1936 Size: 2412 4594 SbfI CC_TGCA'GG Cuts at: 4358 4358 Size: 7006 ScaI AGT'ACT Cuts at: 2034 3806 5254 6966 2034 Size: 1772 1448 1712 2074 Fragments arranged by size: 2074 1772 1712 1448 SfcI C'TryA_G Cuts at: 826 1045 2091 2352 2397 2645 3703 3822 4354 Size: 219 1046 261 45 248 1058 119 532 Cuts at: 4354 5489 6170 6361 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1058 1046 681 532 261 248 219 191 119 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7006 SmaI CCC'GGG Cuts at: 1872 4333 1872 Size: 2461 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2458 5110 6011 6273 820 Size: 197 926 515 2652 901 262 1553 Fragments arranged by size: 2652 1553 926 901 515 262 197 SnaBI TAC'GTA Cuts at: 493 4303 4797 493 Size: 3810 494 2702 Fragments arranged by size: 3810 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7006 SphI G_CATG'C Cuts at: 1132 3140 4364 1132 Size: 2008 1224 3774 Fragments arranged by size: 3774 2008 1224 SspI AAT'ATT Cuts at: 5 52 4930 5 Size: 47 4878 2081 Fragments arranged by size: 4878 2081 47 StuI AGG'CCT Cuts at: 1517 2612 1517 Size: 1095 5911 StyI C'CwwG_G Cuts at: 513 1065 2061 2256 2557 2569 3074 3477 4445 Size: 552 996 195 301 12 505 403 968 Cuts at: 4445 513 Size: 3074 Fragments arranged by size: 3074 996 968 552 505 403 301 195 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2460 2851 3995 5033 5050 5203 5388 Size: 1255 880 391 1144 1038 17 153 185 Cuts at: 5388 5833 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1144 1038 880 445 391 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2032 3804 5252 Size: 274 80 33 51 1443 55 1772 1448 Cuts at: 5252 6964 96 Size: 1712 138 Fragments arranged by size: 1772 1712 1448 1443 274 138 80 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6966 6998 6966 Size: 32 6974 Tth111I GACn'n_nGTC Cuts at: 1316 3404 1316 Size: 2088 4918 XbaI T'CTAG_A Cuts at: 4342 4342 Size: 7006 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2314 2314 Size: 7006 XhoI C'TCGA_G Cuts at: 1943 2458 1943 Size: 515 6491 XmaI C'CCGG_G Cuts at: 1870 4331 1870 Size: 2461 4545 XmnI GAAnn'nnTTC Cuts at: 2034 5135 2034 Size: 3101 3905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2098 4021 4814 276 Size: 53 83 186 936 564 1923 793 2468 Fragments arranged by size: 2468 1923 936 793 564 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI