Restriction map for
pFN21ASDB3800
(Linear) (Six-base) MAPSORT of: KIBB3800.seq Check: 89 from: 1 to: 3969
KIBB3800.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AccI GT'mk_AC
Cuts at: 0 3046 3969
Size: 3046 923
Acc65I G'GTAC_C
Cuts at: 0 1782 2112 3969
Size: 1782 330 1857
Fragments arranged by size:
1857 1782 330
AclI AA'CG_TT
Cuts at: 0 3515 3969
Size: 3515 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 2414 2736 3015 3775 3969
Size: 701 1713 322 279 760 194
Fragments arranged by size:
1713 760 701 322 279 194
AflIII A'CryG_T
Cuts at: 0 3786 3969
Size: 3786 183
AleI CACnn'nnGTG
Cuts at: 0 632 3969
Size: 632 3337
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3385 3417 3969
Size: 3385 32 552
Fragments arranged by size:
3385 552 32
AlwNI CAG_nnn'CTG
Cuts at: 0 413 2820 3417 3969
Size: 413 2407 597 552
Fragments arranged by size:
2407 597 552 413
ApaI G_GGCC'C
Cuts at: 0 3925 3969
Size: 3925 44
ApoI r'AATT_y
Cuts at: 0 315 615 847 1463 1730 2532 3107 3969
Size: 315 300 232 616 267 802 575 862
Fragments arranged by size:
862 802 616 575 315 300 267 232
AvrII C'CTAG_G
Cuts at: 0 592 3969
Size: 592 3377
BamHI G'GATC_C
Cuts at: 0 333 3969
Size: 333 3636
BanI G'GyrC_C
Cuts at: 0 1782 2112 3178 3289 3882 3969
Size: 1782 330 1066 111 593 87
Fragments arranged by size:
1782 1066 593 330 111 87
BanII G_rGCy'C
Cuts at: 0 2014 2085 2092 3925 3969
Size: 2014 71 7 1833 44
Fragments arranged by size:
2014 1833 71 44 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 3969
Size: 35 3934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2683 2717 3510 3544 3969
Size: 2683 34 793 34 425
Fragments arranged by size:
2683 793 425 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3328 3969
Size: 3328 641
BclI T'GATC_A
Cuts at: 0 63 270 3969
Size: 63 207 3699
Fragments arranged by size:
3699 207 63
BfrBI ATG'CAT
Cuts at: 0 156 2604 3969
Size: 156 2448 1365
Fragments arranged by size:
2448 1365 156
BglII A'GATC_T
Cuts at: 0 364 600 1658 3969
Size: 364 236 1058 2311
Fragments arranged by size:
2311 1058 364 236
BlpI GC'TnA_GC
Cuts at: 0 2153 3969
Size: 2153 1816
Bme1580I G_kGCm'C
Cuts at: 0 3925 3969
Size: 3925 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 2275 3787 3969
Size: 2275 1512 182
Fragments arranged by size:
2275 1512 182
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 888 2366 3969
Size: 888 1478 1603
Fragments arranged by size:
1603 1478 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1673 1757 2159 3969
Size: 1673 84 402 1810
Fragments arranged by size:
1810 1673 402 84
Bpu10I CC'TnA_GC
Cuts at: 0 2069 3969
Size: 2069 1900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1958 1986 3426 3969
Size: 1958 28 1440 543
Fragments arranged by size:
1958 1440 543 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 449 3969
Size: 449 3520
BsaAI yAC'GTr
Cuts at: 0 3787 3969
Size: 3787 182
BsaBI GATnn'nnATC
Cuts at: 0 1706 2866 3969
Size: 1706 1160 1103
Fragments arranged by size:
1706 1160 1103
BsaWI w'CCGG_w
Cuts at: 0 692 1785 2649 3969
Size: 692 1093 864 1320
Fragments arranged by size:
1320 1093 864 692
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2037 2067 2132 2162 3969
Size: 2037 30 65 30 1807
Fragments arranged by size:
2037 1807 65 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 3969
Size: 682 3287
BseYI C'CCAG_C
Cuts at: 0 3112 3969
Size: 3112 857
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2713 3969
Size: 2713 1256
BsiHKAI G_wGCw'C
Cuts at: 0 676 2085 2092 3482 3969
Size: 676 1409 7 1390 487
Fragments arranged by size:
1409 1390 676 487 7
BsmI GAATG_Cn'
Cuts at: 0 1145 1240 1974 2606 3969
Size: 1145 95 734 632 1363
Fragments arranged by size:
1363 1145 734 632 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2311 2989 3969
Size: 2311 678 980
Fragments arranged by size:
2311 980 678
Bsp1286I G_dGCh'C
Cuts at: 0 676 2014 2085 2092 3482 3925 3969
Size: 676 1338 71 7 1390 443 44
Fragments arranged by size:
1390 1338 676 443 71 44 7
BspHI T'CATG_A
Cuts at: 0 3011 3969
Size: 3011 958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1018 3969
Size: 1018 2951
BsrDI GCAATG_nn'
Cuts at: 0 2384 3837 3969
Size: 2384 1453 132
Fragments arranged by size:
2384 1453 132
BsrFI r'CCGG_y
Cuts at: 0 3885 3969
Size: 3885 84
BsrGI T'GTAC_A
Cuts at: 0 3219 3969
Size: 3219 750
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3178 3417 3969
Size: 3178 239 552
Fragments arranged by size:
3178 552 239
BstEII G'GTnAC_C
Cuts at: 0 1309 3969
Size: 1309 2660
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2109 3357 3969
Size: 2109 1248 612
Fragments arranged by size:
2109 1248 612
BstYI r'GATC_y
Cuts at: 0 333 364 600 1658 2192 3969
Size: 333 31 236 1058 534 1777
Fragments arranged by size:
1777 1058 534 333 236 31
BstZ17I GTA'TAC
Cuts at: 0 3047 3969
Size: 3047 922
Bsu36I CC'TnA_GG
Cuts at: 0 977 2076 3969
Size: 977 1099 1893
Fragments arranged by size:
1893 1099 977
BtgI C'CryG_G
Cuts at: 0 3554 3585 3894 3969
Size: 3554 31 309 75
Fragments arranged by size:
3554 309 75 31
BtsI GCAGTG_nn'
Cuts at: 0 78 1851 2615 2818 3415 3969
Size: 78 1773 764 203 597 554
Fragments arranged by size:
1773 764 597 554 203 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2834 2869 3969
Size: 2834 35 1100
Fragments arranged by size:
2834 1100 35
DraI TTT'AAA
Cuts at: 0 1040 1298 2129 2525 3969
Size: 1040 258 831 396 1444
Fragments arranged by size:
1444 1040 831 396 258
DraIII CAC_nnn'GTG
Cuts at: 0 2691 3969
Size: 2691 1278
EaeI y'GGCC_r
Cuts at: 0 3834 3969
Size: 3834 135
EarI CTCTTCn'nnn_
Cuts at: 0 2447 3333 3969
Size: 2447 886 636
Fragments arranged by size:
2447 886 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3472 3969
Size: 3472 497
EcoICRI GAG'CTC
Cuts at: 0 2083 2090 3969
Size: 2083 7 1879
Fragments arranged by size:
2083 1879 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1673 1757 2159 2414 2736 3015 3775
Size: 701 972 84 402 255 322 279 760
Cuts at: 3775 3969
Size: 194
Fragments arranged by size:
972 760 701 402 322 279 255 194
84
EcoO109I rG'GnC_Cy
Cuts at: 0 567 3902 3921 3922 3969
Size: 567 3335 19 1 47
Fragments arranged by size:
3335 567 47 19 1
EcoRI G'AATT_C
Cuts at: 0 315 847 3969
Size: 315 532 3122
Fragments arranged by size:
3122 532 315
EcoRV GAT'ATC
Cuts at: 0 2676 3969
Size: 2676 1293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 850 2419 2694 3969
Size: 850 1569 275 1275
Fragments arranged by size:
1569 1275 850 275
HaeII r_GCGC'y
Cuts at: 0 3317 3878 3969
Size: 3317 561 91
Fragments arranged by size:
3317 561 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 2446 2478 3535 3567 3969
Size: 349 32 2065 32 1057 32 402
Fragments arranged by size:
2065 1057 402 349 32 32 32
HincII GTy'rAC
Cuts at: 0 372 905 3969
Size: 372 533 3064
Fragments arranged by size:
3064 533 372
HindIII A'AGCT_T
Cuts at: 0 3716 3969
Size: 3716 253
HpaI GTT'AAC
Cuts at: 0 372 3969
Size: 372 3597
KpnI G_GTAC'C
Cuts at: 0 1786 2116 3969
Size: 1786 330 1853
Fragments arranged by size:
1853 1786 330
MfeI C'AATT_G
Cuts at: 0 3663 3969
Size: 3663 306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 993 2337 2349 2468 3969
Size: 993 1344 12 119 1501
Fragments arranged by size:
1501 1344 993 119 12
MscI TGG'CCA
Cuts at: 0 3836 3969
Size: 3836 133
MslI CAynn'nnrTG
Cuts at: 0 161 632 2107 3969
Size: 161 471 1475 1862
Fragments arranged by size:
1862 1475 471 161
NcoI C'CATG_G
Cuts at: 0 3554 3585 3894 3969
Size: 3554 31 309 75
Fragments arranged by size:
3554 309 75 31
NsiI A_TGCA'T
Cuts at: 0 158 2606 3969
Size: 158 2448 1363
Fragments arranged by size:
2448 1363 158
NspI r_CATG'y
Cuts at: 0 729 1865 2608 3166 3709 3969
Size: 729 1136 743 558 543 260
Fragments arranged by size:
1136 743 729 558 543 260
PacI TTA_AT'TAA
Cuts at: 0 41 3969
Size: 41 3928
PasI CC'CwG_GG
Cuts at: 0 510 3969
Size: 510 3459
PflMI CCAn_nnn'nTGG
Cuts at: 0 2989 3498 3969
Size: 2989 509 471
Fragments arranged by size:
2989 509 471
PpuMI rG'GwC_Cy
Cuts at: 0 567 3969
Size: 567 3402
PsiI TTA'TAA
Cuts at: 0 209 3969
Size: 209 3760
PspOMI G'GGCC_C
Cuts at: 0 3921 3969
Size: 3921 48
PstI C_TGCA'G
Cuts at: 0 1858 2256 3390 3684 3969
Size: 1858 398 1134 294 285
Fragments arranged by size:
1858 1134 398 294 285
SacI G_AGCT'C
Cuts at: 0 2085 2092 3969
Size: 2085 7 1877
Fragments arranged by size:
2085 1877 7
ScaI AGT'ACT
Cuts at: 0 2834 3969
Size: 2834 1135
SfcI C'TryA_G
Cuts at: 0 51 842 961 1344 1854 1890 2252 2638
Size: 51 791 119 383 510 36 362 386
Cuts at: 2638 3386 3539 3680 3969
Size: 748 153 141 289
Fragments arranged by size:
791 748 510 386 383 362 289 153
141 119 51 36
SmlI C'TyrA_G
Cuts at: 0 1973 2001 3405 3969
Size: 1973 28 1404 564
Fragments arranged by size:
1973 1404 564 28
SphI G_CATG'C
Cuts at: 0 2608 3969
Size: 2608 1361
SspI AAT'ATT
Cuts at: 0 1248 3969
Size: 1248 2721
StuI AGG'CCT
Cuts at: 0 2074 3969
Size: 2074 1895
StyI C'CwwG_G
Cuts at: 0 592 1382 2594 2766 2887 3554 3585 3878
Size: 592 790 1212 172 121 667 31 293
Cuts at: 3878 3894 3969
Size: 16 75
Fragments arranged by size:
1212 790 667 592 293 172 121 75
31 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 363 1750 3486 3969
Size: 363 1387 1736 483
Fragments arranged by size:
1736 1387 483 363
TatI w'GTAC_w
Cuts at: 0 2832 3219 3854 3969
Size: 2832 387 635 115
Fragments arranged by size:
2832 635 387 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2132 2164 2644 2676 3773 3805 3969
Size: 2132 32 480 32 1097 32 164
Fragments arranged by size:
2132 1097 480 164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2281 3969
Size: 2281 1688
XbaI T'CTAG_A
Cuts at: 0 1032 3969
Size: 1032 2937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 517 3969
Size: 517 3452
XmnI GAAnn'nnTTC
Cuts at: 0 956 1873 3433 3580 3969
Size: 956 917 1560 147 389
Fragments arranged by size:
1560 956 917 389 147
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaI ApoI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI CspCI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MscI MslI NcoI NsiI NspI PacI PasI PflMI
PpuMI PsiI PspOMI PstI SacI ScaI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaLI
AscI AseI AsiSI AvaI BaeI BbeI BbvCI BglI
BmgBI BmtI BsaHI BsiEI BsiWI BspEI BsrBI BssHII
BssSI BstBI BtgZI ClaI DrdI EagI EcoNI FseI
FspI FspAI KasI MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI PciI PfoI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI XhoI XmaI
ZraI