(Circular) (Six-base) MAPSORT of: pFN21ASDB1510.seq Check: 7341 from: 1 to: 6472 pFN21ASDB1510 6472 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4282 278 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2901 3815 1937 Size: 964 914 4594 Fragments arranged by size: 4594 964 914 Acc65I G'GTAC_C Cuts at: 2612 3793 2612 Size: 1181 5291 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2958 3205 3599 3608 4535 5919 6187 1632 Size: 1326 247 394 9 927 1384 268 1917 Fragments arranged by size: 1917 1384 1326 927 394 268 247 9 AfeI AGC'GCT Cuts at: 2070 2070 Size: 6472 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2606 3224 1184 Size: 1422 618 4432 Fragments arranged by size: 4432 1422 618 AlwNI CAG_nnn'CTG Cuts at: 2041 2566 2743 3061 3228 4215 5787 2041 Size: 525 177 318 167 987 1572 2726 Fragments arranged by size: 2726 1572 987 525 318 177 167 ApaI G_GGCC'C Cuts at: 2129 2306 2656 2896 2129 Size: 177 350 240 5705 Fragments arranged by size: 5705 350 240 177 ApaLI G'TGCA_C Cuts at: 2619 4529 5685 2619 Size: 1910 1156 3406 Fragments arranged by size: 3406 1910 1156 ApoI r'AATT_y Cuts at: 1493 3781 4007 4159 6268 1493 Size: 2288 226 152 2109 1697 Fragments arranged by size: 2288 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2204 3446 3522 3797 1870 Size: 73 261 1242 76 275 4545 Fragments arranged by size: 4545 1242 275 261 76 73 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2612 3793 5248 618 Size: 325 217 627 825 1181 1455 1842 Fragments arranged by size: 1842 1455 1181 825 627 325 217 BanII G_rGCy'C Cuts at: 1391 2129 2205 2211 2306 2647 2656 2896 3791 Size: 738 76 6 95 341 9 240 895 Cuts at: 3791 1391 Size: 4072 Fragments arranged by size: 4072 895 738 341 240 95 76 9 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2045 2079 2446 2456 2480 2490 4663 Size: 34 108 34 367 10 24 10 2173 Cuts at: 4663 4697 1903 Size: 34 3678 Fragments arranged by size: 3678 2173 367 108 34 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 1548 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2252 2310 5083 Size: 107 122 71 631 605 580 58 2773 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 2773 1525 631 605 580 122 107 71 58 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3429 3889 3429 Size: 460 6012 Bme1580I G_kGCm'C Cuts at: 1790 2129 2306 2623 2656 2896 3487 4533 5689 Size: 339 177 317 33 240 591 1046 1156 Cuts at: 5689 1790 Size: 2573 Fragments arranged by size: 2573 1156 1046 591 339 317 240 177 33 BmgBI CAC'GTC Cuts at: 1318 3466 1318 Size: 2148 4324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4203 5161 442 Size: 416 53 83 399 24 2786 958 1753 Fragments arranged by size: 2786 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2588 2692 3444 3476 3728 5132 1239 Size: 1262 87 104 752 32 252 1404 2579 Fragments arranged by size: 2579 1404 1262 752 252 104 87 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4597 5462 5760 4597 Size: 865 298 5309 Fragments arranged by size: 5309 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2392 3099 3505 5135 882 Size: 617 893 707 406 1630 2219 Fragments arranged by size: 2219 1630 893 707 617 406 BsaAI yAC'GTr Cuts at: 493 1204 3235 3769 4263 493 Size: 711 2031 534 494 2702 Fragments arranged by size: 2702 2031 711 534 494 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4279 4661 5296 275 Size: 53 83 186 936 2746 382 635 1451 Fragments arranged by size: 2746 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6247 Fragments arranged by size: 6247 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2498 3605 3728 3731 1186 Size: 1312 1107 123 3 3927 Fragments arranged by size: 3927 1312 1107 123 3 BseYI C'CCAG_C Cuts at: 2041 2059 2163 2344 2749 2771 3023 5675 6171 Size: 18 104 181 405 22 252 2652 496 Cuts at: 6171 2041 Size: 2342 Fragments arranged by size: 2652 2342 496 405 252 181 104 22 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3423 1163 Size: 573 1687 4212 Fragments arranged by size: 4212 1687 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 2123 2314 3630 3922 4683 Size: 275 186 100 22 191 1316 292 761 Cuts at: 4683 5711 1540 Size: 1028 2301 Fragments arranged by size: 2301 1316 1028 761 292 275 191 186 100 22 BsiHKAI G_wGCw'C Cuts at: 2585 2623 2647 4533 4618 5689 2585 Size: 38 24 1886 85 1071 3368 Fragments arranged by size: 3368 1886 1071 85 38 24 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 BsmBI CGTCTCn'nnnn_ Cuts at: 2225 2452 2225 Size: 227 6245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2205 2211 2306 2585 2623 2647 Size: 399 339 76 6 95 279 38 24 Cuts at: 2647 2656 2896 3487 3791 4533 4618 5689 1391 Size: 9 240 591 304 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 591 399 339 304 279 240 95 85 76 38 24 9 6 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2690 3445 3827 844 Size: 1846 755 382 3489 Fragments arranged by size: 3489 1846 755 382 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2552 4967 5141 65 Size: 1183 1304 2415 174 1396 Fragments arranged by size: 2415 1396 1304 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2376 5116 5303 1700 Size: 110 566 2740 187 2869 Fragments arranged by size: 2869 2740 566 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 2455 4532 5544 2455 Size: 2077 1012 3383 Fragments arranged by size: 3383 2077 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6472 BstBI TT'CG_AA Cuts at: 2007 5276 2007 Size: 3269 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstXI CCAn_nnnn'nTGG Cuts at: 3640 3640 Size: 6472 BstYI r'GATC_y Cuts at: 1972 2403 3802 4169 4554 4571 6023 6109 6121 Size: 431 1399 367 385 17 1452 86 12 Cuts at: 6121 6467 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1399 431 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2047 2146 2950 3279 513 Size: 552 957 25 99 804 329 3706 Fragments arranged by size: 3706 957 804 552 329 99 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3079 3331 487 Size: 683 56 1853 252 3628 Fragments arranged by size: 3628 1853 683 252 56 BtsI GCAGTG_nn' Cuts at: 2527 3309 3342 3440 3485 3988 4782 4802 2527 Size: 782 33 98 45 503 794 20 4197 Fragments arranged by size: 4197 794 782 503 98 45 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5479 809 Size: 507 4163 1802 Fragments arranged by size: 4163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2120 2244 Size: 54 1425 50 275 18 268 22 124 Cuts at: 2244 2311 2670 2953 3385 3627 3642 3919 4808 Size: 67 359 283 432 242 15 277 889 Cuts at: 4808 6437 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 432 359 283 277 275 268 242 124 67 54 50 43 22 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2098 2120 2311 3627 3919 1537 Size: 275 286 22 191 1316 292 4090 Fragments arranged by size: 4090 1316 292 286 275 191 22 EarI CTCTTCn'nnn_ Cuts at: 1353 2875 4402 1353 Size: 1522 1527 3423 Fragments arranged by size: 3423 1527 1522 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2222 2384 3261 5059 5433 5579 Size: 1064 138 633 162 877 1798 374 146 Cuts at: 5579 387 Size: 1280 Fragments arranged by size: 1798 1280 1064 877 633 374 162 146 138 EcoICRI GAG'CTC Cuts at: 2645 2645 Size: 6472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2501 2588 2692 2958 3205 3444 3476 Size: 393 869 87 104 266 247 239 32 Cuts at: 3476 3599 3608 3728 4535 5132 5919 6187 1239 Size: 123 9 120 807 597 787 268 1524 Fragments arranged by size: 1524 869 807 787 597 393 268 266 247 239 123 120 104 87 32 9 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2354 Size: 6472 EcoO109I rG'GnC_Cy Cuts at: 2303 2438 2652 2653 2813 2892 2893 2303 Size: 135 214 1 160 79 1 5882 Fragments arranged by size: 5882 214 160 135 79 1 1 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3709 3709 Size: 6472 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 2042 2072 5619 1678 Size: 364 30 3547 2531 Fragments arranged by size: 3547 2531 364 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3098 3130 5113 5145 5187 Size: 32 135 32 1526 32 1983 32 42 Cuts at: 5187 5219 1373 Size: 32 2626 Fragments arranged by size: 2626 1983 1526 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2902 3816 4062 669 Size: 988 281 964 914 246 3079 Fragments arranged by size: 3079 988 964 914 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 2616 3797 2616 Size: 1181 5291 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2430 3141 3643 5586 5770 1305 Size: 1125 711 502 1943 184 2007 Fragments arranged by size: 2007 1943 1125 711 502 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2672 3644 10 Size: 54 1425 343 840 972 2838 Fragments arranged by size: 2838 1425 972 840 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2678 3128 4430 4789 4948 518 Size: 648 861 651 450 1302 359 159 2042 Fragments arranged by size: 2042 1302 861 651 648 450 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2059 2148 2269 2881 3058 3228 3888 Size: 443 10 89 121 612 177 170 660 Cuts at: 3888 4565 5713 5958 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 660 612 443 245 177 170 121 89 10 NaeI GCC'GGC Cuts at: 2378 2378 Size: 6472 NcoI C'CATG_G Cuts at: 513 1065 2022 2950 513 Size: 552 957 928 4035 Fragments arranged by size: 4035 957 928 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NgoMIV G'CCGG_C Cuts at: 2376 2376 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 3830 5375 1132 Size: 97 2601 1545 2229 Fragments arranged by size: 2601 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3297 1348 Size: 1949 4523 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 2566 2743 1108 Size: 1134 324 177 4837 Fragments arranged by size: 4837 1134 324 177 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 6054 6086 4541 Size: 32 1481 32 4927 Fragments arranged by size: 4927 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2813 2813 Size: 6472 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PspOMI G'GGCC_C Cuts at: 2125 2302 2652 2892 2125 Size: 177 350 240 5705 Fragments arranged by size: 5705 350 240 177 PstI C_TGCA'G Cuts at: 830 2058 2522 3057 3213 3824 830 Size: 1228 464 535 156 611 3478 Fragments arranged by size: 3478 1228 611 535 464 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2059 3058 3228 2059 Size: 999 170 5303 Fragments arranged by size: 5303 999 170 SacI G_AGCT'C Cuts at: 2647 2647 Size: 6472 SacII CC_GC'GG Cuts at: 2050 2149 2050 Size: 99 6373 SalI G'TCGA_C Cuts at: 1936 2900 3814 1936 Size: 964 914 4594 Fragments arranged by size: 4594 964 914 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2054 2518 3053 3209 3313 3820 4955 Size: 219 1009 464 535 156 104 507 1135 Cuts at: 4955 5636 5827 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 535 507 464 219 191 156 104 SfiI GGCCn_nnn'nGGCC Cuts at: 2310 2310 Size: 6472 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 3799 1872 Size: 1927 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4576 5477 5739 820 Size: 197 926 2633 901 262 1553 Fragments arranged by size: 2633 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3769 4263 493 Size: 3276 494 2702 Fragments arranged by size: 3276 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 2943 1517 Size: 1426 5046 StyI C'CwwG_G Cuts at: 513 1065 2022 2667 2766 2950 3911 513 Size: 552 957 645 99 184 961 3074 Fragments arranged by size: 3074 961 957 645 552 184 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3037 4499 4516 4669 4854 5299 325 Size: 1255 1457 1462 17 153 185 445 1498 Fragments arranged by size: 1498 1462 1457 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4718 6430 96 Size: 274 80 33 51 1443 2741 1712 138 Fragments arranged by size: 2741 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3271 3303 6432 6464 3271 Size: 32 3129 32 3279 Fragments arranged by size: 3279 3129 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6472 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 3797 1870 Size: 1927 4545 XmnI GAAnn'nnTTC Cuts at: 3567 4601 3567 Size: 1034 5438 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4280 276 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfoI SrfI SwaI XcmI