(Circular) (Six-base) MAPSORT of: pFN21AE2504.seq Check: 6803 from: 1 to: 7747 pFN21AE2504 7747 bp With 182 enzymes: SgfI * February 16, 2012 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2949 3390 5557 278 Size: 53 83 186 936 1413 441 2167 2468 Fragments arranged by size: 2468 2167 1413 936 441 186 83 53 AccI GT'mk_AC Cuts at: 1937 4905 5090 1937 Size: 2968 185 4594 Fragments arranged by size: 4594 2968 185 Acc65I G'GTAC_C Cuts at: 2157 2475 5068 2157 Size: 318 2593 4836 Fragments arranged by size: 4836 2593 318 AclI AA'CG_TT Cuts at: 1563 5874 6247 1563 Size: 4311 373 3063 Fragments arranged by size: 4311 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2539 2609 2807 3141 3395 3646 4067 4449 Size: 907 70 198 334 254 251 421 382 Cuts at: 4449 4671 4820 4863 5021 5810 7194 7462 1632 Size: 222 149 43 158 789 1384 268 1917 Fragments arranged by size: 1917 1384 907 789 421 382 334 268 254 251 222 198 158 149 70 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7550 AflIII A'CryG_T Cuts at: 1203 4671 4788 6646 7623 1203 Size: 3468 117 1858 977 1327 Fragments arranged by size: 3468 1858 1327 977 117 AgeI A'CCGG_T Cuts at: 6578 6578 Size: 7747 AhdI GACnn_n'nnGTC Cuts at: 6476 6476 Size: 7747 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7747 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7747 AlwNI CAG_nnn'CTG Cuts at: 3470 3506 3727 3768 4192 5490 7062 3470 Size: 36 221 41 424 1298 1572 4155 Fragments arranged by size: 4155 1572 1298 424 221 41 36 ApaLI G'TGCA_C Cuts at: 5804 6960 5804 Size: 1156 6591 ApoI r'AATT_y Cuts at: 1493 3017 3892 5021 5056 5282 5434 7543 1493 Size: 1524 875 1129 35 226 152 2109 1697 Fragments arranged by size: 2109 1697 1524 1129 875 226 152 35 AscI GG'CGCG_CC Cuts at: 2056 2056 Size: 7747 AseI AT'TA_AT Cuts at: 160 1032 6301 160 Size: 872 5269 1606 Fragments arranged by size: 5269 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7747 AvaI C'yCGr_G Cuts at: 1870 1943 2525 2899 3197 3248 5072 1870 Size: 73 582 374 298 51 1824 4545 Fragments arranged by size: 4545 1824 582 374 298 73 51 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2141 2174 2141 Size: 33 7714 BamHI G'GATC_C Cuts at: 3604 4366 4617 5077 5444 3604 Size: 762 251 460 367 5907 Fragments arranged by size: 5907 762 460 367 251 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2157 2199 2475 2873 3983 Size: 325 217 627 370 42 276 398 1110 Cuts at: 3983 5068 6523 618 Size: 1085 1455 1842 Fragments arranged by size: 1842 1455 1110 1085 627 398 370 325 276 217 42 BanII G_rGCy'C Cuts at: 1391 5066 1391 Size: 3675 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2007 2270 2333 2905 3652 4625 928 Size: 971 108 263 63 572 747 973 4050 Fragments arranged by size: 4050 973 971 747 572 263 108 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2516 2550 2851 2885 5938 5972 1903 Size: 34 579 34 301 34 3053 34 3678 Fragments arranged by size: 3678 3053 579 301 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3915 5639 6849 3915 Size: 1724 1210 4813 Fragments arranged by size: 4813 1724 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7747 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2720 3098 3278 Size: 107 122 71 631 605 1048 378 180 Cuts at: 3278 6358 136 Size: 3080 1525 Fragments arranged by size: 3080 1525 1048 631 605 378 180 122 107 71 BglII A'GATC_T Cuts at: 7742 7742 Size: 7747 BlpI GC'TnA_GC Cuts at: 4106 5164 4106 Size: 1058 6689 Bme1580I G_kGCm'C Cuts at: 1790 2878 5808 6964 1790 Size: 1088 2930 1156 2573 Fragments arranged by size: 2930 2573 1156 1088 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7747 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2949 5478 6436 Size: 416 53 83 399 24 1532 2529 958 Cuts at: 6436 442 Size: 1753 Fragments arranged by size: 2529 1753 1532 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7747 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2914 2914 Size: 7747 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 3365 3662 3749 3947 6407 1239 Size: 1059 1067 297 87 198 2460 2579 Fragments arranged by size: 2579 2460 1067 1059 297 198 87 Bpu10I CC'TnA_GC Cuts at: 4337 4989 4337 Size: 652 7095 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 3023 5872 6737 7035 2531 Size: 492 2849 865 298 3243 Fragments arranged by size: 3243 2849 865 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4711 4737 6410 882 Size: 617 3212 26 1673 2219 Fragments arranged by size: 3212 2219 1673 617 26 BsaAI yAC'GTr Cuts at: 493 1204 2376 5538 493 Size: 711 1172 3162 2702 Fragments arranged by size: 3162 2702 1172 711 BsaBI GATnn'nnATC Cuts at: 2111 7741 2111 Size: 5630 2117 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2946 3387 5554 5936 Size: 53 83 186 936 1413 441 2167 382 Cuts at: 5936 6571 275 Size: 635 1451 Fragments arranged by size: 2167 1451 1413 936 635 441 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2103 2991 6180 6578 6852 6999 7410 2103 Size: 888 3189 398 274 147 411 2440 Fragments arranged by size: 3189 2440 888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 3585 3615 4691 Size: 30 165 30 747 30 1405 30 1076 Cuts at: 4691 4721 1178 Size: 30 4204 Fragments arranged by size: 4204 1405 1076 747 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2295 2522 3139 3606 1186 Size: 1109 227 617 467 5327 Fragments arranged by size: 5327 1109 617 467 227 BseYI C'CCAG_C Cuts at: 2512 4613 6950 7446 2512 Size: 2101 2337 496 2813 Fragments arranged by size: 2813 2337 2101 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 2920 4298 1163 Size: 573 405 779 1378 4612 Fragments arranged by size: 4612 1378 779 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5197 5958 6986 1540 Size: 275 186 3196 761 1028 2301 Fragments arranged by size: 3196 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3843 5808 5893 6964 3843 Size: 1965 85 1071 4626 Fragments arranged by size: 4626 1965 1071 85 BsmI GAATG_Cn' Cuts at: 2900 4541 5258 5351 2900 Size: 1641 717 93 5296 Fragments arranged by size: 5296 1641 717 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3394 4740 3394 Size: 1346 6401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2878 3843 5066 5808 5893 6964 1391 Size: 399 1088 965 1223 742 85 1071 2174 Fragments arranged by size: 2174 1223 1088 1071 965 742 399 85 BspEI T'CCGG_A Cuts at: 2103 7410 2103 Size: 5307 2440 BspHI T'CATG_A Cuts at: 3846 5636 7366 3846 Size: 1790 1730 4227 Fragments arranged by size: 4227 1790 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4314 5102 844 Size: 3470 788 3489 Fragments arranged by size: 3489 3470 788 BsrBI CCG'CTC Cuts at: 1394 1823 2052 4255 5634 1394 Size: 429 229 2203 1379 3507 Fragments arranged by size: 3507 2203 1379 429 229 BsrDI GCAATG_nn' Cuts at: 65 1248 6242 6416 65 Size: 1183 4994 174 1396 Fragments arranged by size: 4994 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6391 6578 1700 Size: 110 4581 187 2869 Fragments arranged by size: 4581 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7747 BssHII G'CGCG_C Cuts at: 1925 2056 1925 Size: 131 7616 BssSI C'ACGA_G Cuts at: 5807 6819 5807 Size: 1012 6735 BstAPI GCAn_nnn'nTGC Cuts at: 794 2073 2354 2818 794 Size: 1279 281 464 5723 Fragments arranged by size: 5723 1279 464 281 BstBI TT'CG_AA Cuts at: 6551 6551 Size: 7747 BstEII G'GTnAC_C Cuts at: 1184 3004 1184 Size: 1820 5927 BstXI CCAn_nnnn'nTGG Cuts at: 2968 4235 2968 Size: 1267 6480 BstYI r'GATC_y Cuts at: 1972 3186 3604 4119 4188 4195 4366 4617 4686 Size: 1214 418 515 69 7 171 251 69 Cuts at: 4686 5077 5444 5829 5846 7298 7384 7396 7742 Size: 391 367 385 17 1452 86 12 346 Cuts at: 7742 1972 Size: 1977 Fragments arranged by size: 1977 1452 1214 515 418 391 385 367 346 251 171 86 69 69 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2004 3096 4059 513 Size: 552 939 1092 963 4201 Fragments arranged by size: 4201 1092 963 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2649 2937 487 Size: 683 56 1423 288 5297 Fragments arranged by size: 5297 1423 683 288 56 BtsI GCAGTG_nn' Cuts at: 2949 3963 5263 6057 6077 2949 Size: 1014 1300 794 20 4619 Fragments arranged by size: 4619 1300 1014 794 20 ClaI AT'CG_AT Cuts at: 1554 4374 4506 1554 Size: 2820 132 4795 Fragments arranged by size: 4795 2820 132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4591 4626 529 Size: 35 4027 35 3650 Fragments arranged by size: 4027 3650 35 35 DraI TTT'AAA Cuts at: 5051 5051 Size: 7747 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4499 6754 809 Size: 507 3183 2255 1802 Fragments arranged by size: 3183 2255 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 3102 3270 Size: 54 1425 50 275 18 279 993 168 Cuts at: 3270 3940 5194 6083 7712 8 Size: 670 1254 889 1629 43 Fragments arranged by size: 1629 1425 1254 993 889 670 279 275 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5194 1537 Size: 275 3382 4090 Fragments arranged by size: 4090 3382 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3945 5677 1353 Size: 2592 1732 3423 Fragments arranged by size: 3423 2592 1732 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3102 3382 6334 6708 6854 Size: 1064 138 435 1078 280 2952 374 146 Cuts at: 6854 387 Size: 1280 Fragments arranged by size: 2952 1280 1078 1064 435 374 280 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2539 2609 2807 3141 3365 3395 Size: 393 666 241 70 198 334 224 30 Cuts at: 3395 3646 3662 3749 3947 4067 4449 4671 4820 Size: 251 16 87 198 120 382 222 149 Cuts at: 4820 4863 5021 5810 6407 7194 7462 1239 Size: 43 158 789 597 787 268 1524 Fragments arranged by size: 1524 789 787 666 597 393 382 334 268 251 241 224 222 198 198 158 149 120 87 70 43 30 16 EcoNI CCTnn'n_nnAGG Cuts at: 4145 4625 4145 Size: 480 7267 EcoO109I rG'GnC_Cy Cuts at: 3765 4015 3765 Size: 250 7497 EcoRI G'AATT_C Cuts at: 3017 5056 5434 3017 Size: 2039 378 5330 Fragments arranged by size: 5330 2039 378 EcoRV GAT'ATC Cuts at: 4510 4510 Size: 7747 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4048 4111 4796 4048 Size: 63 685 6999 Fragments arranged by size: 6999 685 63 FspI TGC'GCA Cuts at: 3633 6253 3633 Size: 2620 5127 HaeII r_GCGC'y Cuts at: 1678 3093 4658 6894 1678 Size: 1415 1565 2236 2531 Fragments arranged by size: 2531 2236 1565 1415 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 3178 3210 3961 Size: 32 135 32 638 32 936 32 751 Cuts at: 3961 3993 4180 4212 4691 4723 6388 6420 6462 Size: 32 187 32 479 32 1665 32 42 Cuts at: 6462 6494 1373 Size: 32 2626 Fragments arranged by size: 2626 1665 936 751 638 479 187 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3261 4381 4417 5091 5337 669 Size: 988 281 1323 1120 36 674 246 3079 Fragments arranged by size: 3079 1323 1120 988 674 281 246 36 HindIII A'AGCT_T Cuts at: 748 2702 748 Size: 1954 5793 HpaI GTT'AAC Cuts at: 5337 5337 Size: 7747 KpnI G_GTAC'C Cuts at: 2161 2479 5072 2161 Size: 318 2593 4836 Fragments arranged by size: 4836 2593 318 MfeI C'AATT_G Cuts at: 5346 5346 Size: 7747 MluI A'CGCG_T Cuts at: 7623 7623 Size: 7747 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2388 3201 6861 7045 1305 Size: 1083 813 3660 184 2007 Fragments arranged by size: 3660 2007 1083 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2111 3942 10 Size: 54 1425 343 279 1831 3815 Fragments arranged by size: 3815 1831 1425 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 4025 4319 4409 5705 6064 6223 Size: 648 951 1908 294 90 1296 359 159 Cuts at: 6223 518 Size: 2042 Fragments arranged by size: 2042 1908 1296 951 648 359 294 159 90 MspA1I CmG'CkG Cuts at: 1606 2270 2472 2908 3599 5163 5840 6988 7233 Size: 664 202 436 691 1564 677 1148 245 Cuts at: 7233 1606 Size: 2120 Fragments arranged by size: 2120 1564 1148 691 677 664 436 245 202 NcoI C'CATG_G Cuts at: 513 1065 2004 4059 513 Size: 552 939 2055 4201 Fragments arranged by size: 4201 2055 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7747 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7747 NotI GC'GGCC_GC Cuts at: 5194 5194 Size: 7747 NspI r_CATG'y Cuts at: 1132 1229 3638 4675 4792 5105 6650 1132 Size: 97 2409 1037 117 313 1545 2229 Fragments arranged by size: 2409 2229 1545 1037 313 117 97 PasI CC'CwG_GG Cuts at: 1348 4627 1348 Size: 3279 4468 PciI A'CATG_T Cuts at: 4671 4788 6646 4671 Size: 117 1858 5772 Fragments arranged by size: 5772 1858 117 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7747 PfoI T'CCnGG_A Cuts at: 3209 3381 3639 4191 4595 3209 Size: 172 258 552 404 6361 Fragments arranged by size: 6361 552 404 258 172 PmeI GTTT'AAAC Cuts at: 5051 5051 Size: 7747 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5816 5848 7329 7361 5816 Size: 32 1481 32 6202 Fragments arranged by size: 6202 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3765 4015 3765 Size: 250 7497 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7747 PsiI TTA'TAA Cuts at: 5317 5317 Size: 7747 PstI C_TGCA'G Cuts at: 830 3723 5099 830 Size: 2893 1376 3478 Fragments arranged by size: 3478 2893 1376 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7747 PvuII CAG'CTG Cuts at: 2908 3599 2908 Size: 691 7056 SalI G'TCGA_C Cuts at: 1936 5089 1936 Size: 3153 4594 SbfI CC_TGCA'GG Cuts at: 5099 5099 Size: 7747 ScaI AGT'ACT Cuts at: 5995 7707 5995 Size: 1712 6035 SfcI C'TryA_G Cuts at: 826 1045 2568 3719 4327 5095 6230 6911 7102 Size: 219 1523 1151 608 768 1135 681 191 Cuts at: 7102 826 Size: 1471 Fragments arranged by size: 1523 1471 1151 1135 768 681 608 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7747 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7747 SmaI CCC'GGG Cuts at: 1872 5074 1872 Size: 3202 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 3038 5851 6752 7014 820 Size: 197 926 603 492 2813 901 262 1553 Fragments arranged by size: 2813 1553 926 901 603 492 262 197 SnaBI TAC'GTA Cuts at: 493 5538 493 Size: 5045 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7747 SphI G_CATG'C Cuts at: 1132 5105 1132 Size: 3973 3774 SspI AAT'ATT Cuts at: 5 52 5671 5 Size: 47 5619 2081 Fragments arranged by size: 5619 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7747 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 3443 3673 3822 4059 4095 Size: 552 939 87 1352 230 149 237 36 Cuts at: 4095 4284 4780 4830 5186 513 Size: 189 496 50 356 3074 Fragments arranged by size: 3074 1352 939 552 496 356 237 230 189 149 87 50 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2324 2862 3001 5774 5791 5944 6129 Size: 1255 744 538 139 2773 17 153 185 Cuts at: 6129 6574 325 Size: 445 1498 Fragments arranged by size: 2773 1498 1255 744 538 445 185 153 139 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2529 4968 5993 Size: 274 80 33 51 1443 552 2439 1025 Cuts at: 5993 7705 96 Size: 1712 138 Fragments arranged by size: 2439 1712 1443 1025 552 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3422 3454 3585 3617 3889 3921 7707 7739 3422 Size: 32 131 32 272 32 3786 32 3430 Fragments arranged by size: 3786 3430 272 131 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4703 1316 Size: 3387 4360 XbaI T'CTAG_A Cuts at: 5083 5083 Size: 7747 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 3094 3307 3676 2306 Size: 788 213 369 6377 Fragments arranged by size: 6377 788 369 213 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7747 XmaI C'CCGG_G Cuts at: 1870 5072 1870 Size: 3202 4545 XmnI GAAnn'nnTTC Cuts at: 3659 5876 3659 Size: 2217 5530 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2947 3388 5555 276 Size: 53 83 186 936 1413 441 2167 2468 Fragments arranged by size: 2468 2167 1413 936 441 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI