Restriction map for
pFN21AE2504
(Linear) (Six-base) MAPSORT of: KIEE2504.seq Check: 8729 from: 1 to: 3042
KIEE2504.orf
With 182 enzymes: SgfI *
February 16, 2012 13:36 ..
AatII G_ACGT'C
Cuts at: 0 944 1385 3042
Size: 944 441 1657
Fragments arranged by size:
1657 944 441
AccI GT'mk_AC
Cuts at: 0 2900 3042
Size: 2900 142
Acc65I G'GTAC_C
Cuts at: 0 152 470 3042
Size: 152 318 2572
Fragments arranged by size:
2572 318 152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 604 802 1136 1390 1641 2062 2444
Size: 534 70 198 334 254 251 421 382
Cuts at: 2444 2666 2815 2858 3016 3042
Size: 222 149 43 158 26
Fragments arranged by size:
534 421 382 334 254 251 222 198
158 149 70 43 26
AflIII A'CryG_T
Cuts at: 0 2666 2783 3042
Size: 2666 117 259
Fragments arranged by size:
2666 259 117
AlwNI CAG_nnn'CTG
Cuts at: 0 1465 1501 1722 1763 2187 3042
Size: 1465 36 221 41 424 855
Fragments arranged by size:
1465 855 424 221 41 36
ApoI r'AATT_y
Cuts at: 0 1012 1887 3016 3042
Size: 1012 875 1129 26
Fragments arranged by size:
1129 1012 875 26
AscI GG'CGCG_CC
Cuts at: 0 51 3042
Size: 51 2991
AvaI C'yCGr_G
Cuts at: 0 520 894 1192 1243 3042
Size: 520 374 298 51 1799
Fragments arranged by size:
1799 520 374 298 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 136 169 3042
Size: 136 33 2873
Fragments arranged by size:
2873 136 33
BamHI G'GATC_C
Cuts at: 0 1599 2361 2612 3042
Size: 1599 762 251 430
Fragments arranged by size:
1599 762 430 251
BanI G'GyrC_C
Cuts at: 0 152 194 470 868 1978 3042
Size: 152 42 276 398 1110 1064
Fragments arranged by size:
1110 1064 398 276 152 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 2 265 328 900 1647 2620 3042
Size: 2 263 63 572 747 973 422
Fragments arranged by size:
973 747 572 422 263 63 2
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 511 545 846 880 3042
Size: 511 34 301 34 2162
Fragments arranged by size:
2162 511 301 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1910 3042
Size: 1910 1132
BglI GCCn_nnn'nGGC
Cuts at: 0 715 1093 1273 3042
Size: 715 378 180 1769
Fragments arranged by size:
1769 715 378 180
BlpI GC'TnA_GC
Cuts at: 0 2101 3042
Size: 2101 941
Bme1580I G_kGCm'C
Cuts at: 0 873 3042
Size: 873 2169
BmrI ACTGGGnnnn_n'
Cuts at: 0 944 3042
Size: 944 2098
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 909 3042
Size: 909 2133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1360 1657 1744 1942 3042
Size: 293 1067 297 87 198 1100
Fragments arranged by size:
1100 1067 297 293 198 87
Bpu10I CC'TnA_GC
Cuts at: 0 2332 2984 3042
Size: 2332 652 58
Fragments arranged by size:
2332 652 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1018 3042
Size: 526 492 2024
Fragments arranged by size:
2024 526 492
BsaI GGTCTCn'nnnn_
Cuts at: 0 2706 2732 3042
Size: 2706 26 310
Fragments arranged by size:
2706 310 26
BsaAI yAC'GTr
Cuts at: 0 371 3042
Size: 371 2671
BsaBI GATnn'nnATC
Cuts at: 0 106 3042
Size: 106 2936
BsaHI Gr'CG_yC
Cuts at: 0 941 1382 3042
Size: 941 441 1660
Fragments arranged by size:
1660 941 441
BsaWI w'CCGG_w
Cuts at: 0 98 986 3042
Size: 98 888 2056
Fragments arranged by size:
2056 888 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 1580 1610 2686 2716 3042
Size: 145 30 1405 30 1076 30 326
Fragments arranged by size:
1405 1076 326 145 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 290 517 1134 1601 3042
Size: 290 227 617 467 1441
Fragments arranged by size:
1441 617 467 290 227
BseYI C'CCAG_C
Cuts at: 0 507 2608 3042
Size: 507 2101 434
Fragments arranged by size:
2101 507 434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 915 2293 3042
Size: 136 779 1378 749
Fragments arranged by size:
1378 779 749 136
BsiHKAI G_wGCw'C
Cuts at: 0 1838 3042
Size: 1838 1204
BsmI GAATG_Cn'
Cuts at: 0 895 2536 3042
Size: 895 1641 506
Fragments arranged by size:
1641 895 506
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1389 2735 3042
Size: 1389 1346 307
Fragments arranged by size:
1389 1346 307
Bsp1286I G_dGCh'C
Cuts at: 0 873 1838 3042
Size: 873 965 1204
Fragments arranged by size:
1204 965 873
BspEI T'CCGG_A
Cuts at: 0 98 3042
Size: 98 2944
BspHI T'CATG_A
Cuts at: 0 1841 3042
Size: 1841 1201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2309 3042
Size: 2309 733
BsrBI CCG'CTC
Cuts at: 0 47 2250 3042
Size: 47 2203 792
Fragments arranged by size:
2203 792 47
BssHII G'CGCG_C
Cuts at: 0 51 3042
Size: 51 2991
BstAPI GCAn_nnn'nTGC
Cuts at: 0 68 349 813 3042
Size: 68 281 464 2229
Fragments arranged by size:
2229 464 281 68
BstEII G'GTnAC_C
Cuts at: 0 999 3042
Size: 999 2043
BstXI CCAn_nnnn'nTGG
Cuts at: 0 963 2230 3042
Size: 963 1267 812
Fragments arranged by size:
1267 963 812
BstYI r'GATC_y
Cuts at: 0 1181 1599 2114 2183 2190 2361 2612 2681
Size: 1181 418 515 69 7 171 251 69
Cuts at: 2681 3042
Size: 361
Fragments arranged by size:
1181 515 418 361 251 171 69 69
7
BtgI C'CryG_G
Cuts at: 0 1091 2054 3042
Size: 1091 963 988
Fragments arranged by size:
1091 988 963
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 644 932 3042
Size: 644 288 2110
Fragments arranged by size:
2110 644 288
BtsI GCAGTG_nn'
Cuts at: 0 944 1958 3042
Size: 944 1014 1084
Fragments arranged by size:
1084 1014 944
ClaI AT'CG_AT
Cuts at: 0 2369 2501 3042
Size: 2369 132 541
Fragments arranged by size:
2369 541 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2586 2621 3042
Size: 2586 35 421
Fragments arranged by size:
2586 421 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2494 3042
Size: 2494 548
EaeI y'GGCC_r
Cuts at: 0 104 1097 1265 1935 3042
Size: 104 993 168 670 1107
Fragments arranged by size:
1107 993 670 168 104
EarI CTCTTCn'nnn_
Cuts at: 0 1940 3042
Size: 1940 1102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1097 1377 3042
Size: 19 1078 280 1665
Fragments arranged by size:
1665 1078 280 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 534 604 802 1136 1360 1390 1641
Size: 293 241 70 198 334 224 30 251
Cuts at: 1641 1657 1744 1942 2062 2444 2666 2815 2858
Size: 16 87 198 120 382 222 149 43
Cuts at: 2858 3016 3042
Size: 158 26
Fragments arranged by size:
382 334 293 251 241 224 222 198
198 158 149 120 87 70 43 30
26 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2140 2620 3042
Size: 2140 480 422
Fragments arranged by size:
2140 480 422
EcoO109I rG'GnC_Cy
Cuts at: 0 1760 2010 3042
Size: 1760 250 1032
Fragments arranged by size:
1760 1032 250
EcoRI G'AATT_C
Cuts at: 0 1012 3042
Size: 1012 2030
EcoRV GAT'ATC
Cuts at: 0 2505 3042
Size: 2505 537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2043 2106 2791 3042
Size: 2043 63 685 251
Fragments arranged by size:
2043 685 251 63
FspI TGC'GCA
Cuts at: 0 1628 3042
Size: 1628 1414
HaeII r_GCGC'y
Cuts at: 0 1088 2653 3042
Size: 1088 1565 389
Fragments arranged by size:
1565 1088 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 1173 1205 1956 1988 2175 2207
Size: 205 32 936 32 751 32 187 32
Cuts at: 2207 2686 2718 3042
Size: 479 32 324
Fragments arranged by size:
936 751 479 324 205 187 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1256 2376 2412 3042
Size: 1256 1120 36 630
Fragments arranged by size:
1256 1120 630 36
HindIII A'AGCT_T
Cuts at: 0 697 3042
Size: 697 2345
KpnI G_GTAC'C
Cuts at: 0 156 474 3042
Size: 156 318 2568
Fragments arranged by size:
2568 318 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1196 3042
Size: 383 813 1846
Fragments arranged by size:
1846 813 383
MscI TGG'CCA
Cuts at: 0 106 1937 3042
Size: 106 1831 1105
Fragments arranged by size:
1831 1105 106
MslI CAynn'nnrTG
Cuts at: 0 112 2020 2314 2404 3042
Size: 112 1908 294 90 638
Fragments arranged by size:
1908 638 294 112 90
MspA1I CmG'CkG
Cuts at: 0 265 467 903 1594 3042
Size: 265 202 436 691 1448
Fragments arranged by size:
1448 691 436 265 202
NcoI C'CATG_G
Cuts at: 0 2054 3042
Size: 2054 988
NspI r_CATG'y
Cuts at: 0 1633 2670 2787 3042
Size: 1633 1037 117 255
Fragments arranged by size:
1633 1037 255 117
PasI CC'CwG_GG
Cuts at: 0 2622 3042
Size: 2622 420
PciI A'CATG_T
Cuts at: 0 2666 2783 3042
Size: 2666 117 259
Fragments arranged by size:
2666 259 117
PfoI T'CCnGG_A
Cuts at: 0 1204 1376 1634 2186 2590 3042
Size: 1204 172 258 552 404 452
Fragments arranged by size:
1204 552 452 404 258 172
PpuMI rG'GwC_Cy
Cuts at: 0 1760 2010 3042
Size: 1760 250 1032
Fragments arranged by size:
1760 1032 250
PstI C_TGCA'G
Cuts at: 0 1718 3042
Size: 1718 1324
PvuII CAG'CTG
Cuts at: 0 903 1594 3042
Size: 903 691 1448
Fragments arranged by size:
1448 903 691
SfcI C'TryA_G
Cuts at: 0 563 1714 2322 3042
Size: 563 1151 608 720
Fragments arranged by size:
1151 720 608 563
SmlI C'TyrA_G
Cuts at: 0 541 1033 3042
Size: 541 492 2009
Fragments arranged by size:
2009 541 492
StyI C'CwwG_G
Cuts at: 0 86 1438 1668 1817 2054 2090 2279 2775
Size: 86 1352 230 149 237 36 189 496
Cuts at: 2775 2825 3042
Size: 50 217
Fragments arranged by size:
1352 496 237 230 217 189 149 86
50 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 857 996 3042
Size: 319 538 139 2046
Fragments arranged by size:
2046 538 319 139
TatI w'GTAC_w
Cuts at: 0 524 2963 3042
Size: 524 2439 79
Fragments arranged by size:
2439 524 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1417 1449 1580 1612 1884 1916 3042
Size: 1417 32 131 32 272 32 1126
Fragments arranged by size:
1417 1126 272 131 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2698 3042
Size: 2698 344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 1089 1302 1671 3042
Size: 301 788 213 369 1371
Fragments arranged by size:
1371 788 369 301 213
XmnI GAAnn'nnTTC
Cuts at: 0 1654 3042
Size: 1654 1388
ZraI GAC'GTC
Cuts at: 0 942 1383 3042
Size: 942 441 1659
Fragments arranged by size:
1659 942 441
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AlwNI ApoI AscI
AvaI BaeI BamHI BanI BbsI BcgI BciVI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NcoI NspI
PasI PciI PfoI PpuMI PstI PvuII SfcI SmlI
StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AseI AsiSI AvrII BanII BbeI
BbvCI BclI BfrBI BglII BmgBI BmtI BsiEI BsiWI
BsrDI BsrFI BsrGI BssSI BstBI BstZ17I Bsu36I DraI
DraIII EagI EcoICRI FseI FspAI HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmaI