(Circular) (Six-base) MAPSORT of: pFN21ASDB0062.seq Check: 2425 from: 1 to: 6739 pFN21ASDB0062 6739 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4549 278 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4082 1937 Size: 2145 4594 Acc65I G'GTAC_C Cuts at: 4060 4060 Size: 6739 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3163 3776 4802 6186 6454 1632 Size: 1531 613 1026 1384 268 1917 Fragments arranged by size: 1917 1531 1384 1026 613 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6542 AflIII A'CryG_T Cuts at: 1203 5638 6615 1203 Size: 4435 977 1327 Fragments arranged by size: 4435 1327 977 AgeI A'CCGG_T Cuts at: 5570 5570 Size: 6739 AhdI GACnn_n'nnGTC Cuts at: 5468 5468 Size: 6739 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2313 1184 Size: 1129 5610 AlwNI CAG_nnn'CTG Cuts at: 3080 4482 6054 3080 Size: 1402 1572 3765 Fragments arranged by size: 3765 1572 1402 ApaLI G'TGCA_C Cuts at: 4796 5952 4796 Size: 1156 5583 ApoI r'AATT_y Cuts at: 1493 2227 2814 2879 3195 3404 4048 4274 4426 Size: 734 587 65 316 209 644 226 152 Cuts at: 4426 6535 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 734 644 587 316 226 209 152 65 AseI AT'TA_AT Cuts at: 160 1032 2390 2475 5293 160 Size: 872 1358 85 2818 1606 Fragments arranged by size: 2818 1606 1358 872 85 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 3513 4064 1870 Size: 73 1570 551 4545 Fragments arranged by size: 4545 1570 551 73 BamHI G'GATC_C Cuts at: 4069 4436 4069 Size: 367 6372 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2860 4060 5515 618 Size: 325 217 627 1073 1200 1455 1842 Fragments arranged by size: 1842 1455 1200 1073 627 325 217 BanII G_rGCy'C Cuts at: 1391 4058 1391 Size: 2667 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4017 928 Size: 971 2118 3650 Fragments arranged by size: 3650 2118 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4930 4964 1903 Size: 34 2993 34 3678 Fragments arranged by size: 3678 2993 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3268 4631 5841 3268 Size: 1363 1210 4166 Fragments arranged by size: 4166 1363 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6739 BfrBI ATG'CAT Cuts at: 3499 3499 Size: 6739 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5350 136 Size: 107 122 71 631 605 3678 1525 Fragments arranged by size: 3678 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6734 6734 Size: 6739 BlpI GC'TnA_GC Cuts at: 4156 4156 Size: 6739 Bme1580I G_kGCm'C Cuts at: 1790 4800 5956 1790 Size: 3010 1156 2573 Fragments arranged by size: 3010 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6739 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2050 4470 5428 Size: 416 53 83 399 24 633 2420 958 Cuts at: 5428 442 Size: 1753 Fragments arranged by size: 2420 1753 958 633 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3957 1056 Size: 2901 3838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2993 3345 5399 1239 Size: 1754 352 2054 2579 Fragments arranged by size: 2579 2054 1754 352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2107 2331 3873 4864 5729 6027 2107 Size: 224 1542 991 865 298 2819 Fragments arranged by size: 2819 1542 991 865 298 224 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3918 5402 882 Size: 617 2419 1484 2219 Fragments arranged by size: 2419 2219 1484 617 BsaAI yAC'GTr Cuts at: 493 1204 4036 4530 493 Size: 711 2832 494 2702 Fragments arranged by size: 2832 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2787 6733 2787 Size: 3946 2793 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 4546 4928 5563 Size: 53 83 186 936 549 2464 382 635 Cuts at: 5563 275 Size: 1451 Fragments arranged by size: 2464 1451 936 635 549 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5172 5570 5844 5991 6402 5172 Size: 398 274 147 411 5509 Fragments arranged by size: 5509 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2386 2416 1178 Size: 30 165 30 983 30 5501 Fragments arranged by size: 5501 983 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3311 3552 1186 Size: 2125 241 4373 Fragments arranged by size: 4373 2125 241 BseYI C'CCAG_C Cuts at: 3840 3963 5942 6438 3840 Size: 123 1979 496 4141 Fragments arranged by size: 4141 1979 496 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6166 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4800 4885 5956 4800 Size: 85 1071 5583 Fragments arranged by size: 5583 1071 85 BsmI GAATG_Cn' Cuts at: 3499 4250 4343 3499 Size: 751 93 5895 Fragments arranged by size: 5895 751 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2089 2089 Size: 6739 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4058 4800 4885 5956 1391 Size: 399 2268 742 85 1071 2174 Fragments arranged by size: 2268 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 4628 6358 4628 Size: 1730 5009 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4094 844 Size: 3250 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4626 1394 Size: 429 2803 3507 Fragments arranged by size: 3507 2803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3526 5234 5408 65 Size: 1183 2278 1708 174 1396 Fragments arranged by size: 2278 1708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5383 5570 1700 Size: 110 3573 187 2869 Fragments arranged by size: 3573 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6739 BssSI C'ACGA_G Cuts at: 4799 5811 4799 Size: 1012 5727 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6739 BstBI TT'CG_AA Cuts at: 2007 5543 2007 Size: 3536 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6739 BstXI CCAn_nnnn'nTGG Cuts at: 3149 3740 3848 3149 Size: 591 108 6040 Fragments arranged by size: 6040 591 108 BstYI r'GATC_y Cuts at: 1972 2378 4069 4436 4821 4838 6290 6376 6388 Size: 406 1691 367 385 17 1452 86 12 Cuts at: 6388 6734 1972 Size: 346 1977 Fragments arranged by size: 1977 1691 1452 406 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3294 3294 Size: 6739 BtgI C'CryG_G Cuts at: 513 1065 2258 513 Size: 552 1193 4994 Fragments arranged by size: 4994 1193 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6000 Fragments arranged by size: 6000 683 56 BtsI GCAGTG_nn' Cuts at: 4255 5049 5069 4255 Size: 794 20 5925 Fragments arranged by size: 5925 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6739 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 3069 4043 3069 Size: 974 5765 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5746 809 Size: 507 4430 1802 Fragments arranged by size: 4430 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3044 4186 5075 Size: 54 1425 50 275 18 1214 1142 889 Cuts at: 5075 6704 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1214 1142 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2817 3182 3921 4669 1353 Size: 1464 365 739 748 3423 Fragments arranged by size: 3423 1464 748 739 365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5326 5700 5846 387 Size: 1064 138 3737 374 146 1280 Fragments arranged by size: 3737 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2993 3163 3345 3776 4802 5399 6186 Size: 393 1361 170 182 431 1026 597 787 Cuts at: 6186 6454 1239 Size: 268 1524 Fragments arranged by size: 1524 1361 1026 787 597 431 393 268 182 170 EcoO109I rG'GnC_Cy Cuts at: 2399 2399 Size: 6739 EcoRI G'AATT_C Cuts at: 4048 4426 4048 Size: 378 6361 EcoRV GAT'ATC Cuts at: 2986 3424 2986 Size: 438 6301 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3234 3781 3860 3234 Size: 547 79 6113 Fragments arranged by size: 6113 547 79 FspI TGC'GCA Cuts at: 5245 5245 Size: 6739 HaeII r_GCGC'y Cuts at: 1678 5886 1678 Size: 4208 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2722 2754 5380 5412 5454 Size: 32 135 32 1150 32 2626 32 42 Cuts at: 5454 5486 1373 Size: 32 2626 Fragments arranged by size: 2626 2626 1150 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2047 4083 4329 669 Size: 988 281 109 2036 246 3079 Fragments arranged by size: 3079 2036 988 281 246 109 HindIII A'AGCT_T Cuts at: 748 2536 748 Size: 1788 4951 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KpnI G_GTAC'C Cuts at: 4064 4064 Size: 6739 MfeI C'AATT_G Cuts at: 4338 4338 Size: 6739 MluI A'CGCG_T Cuts at: 6615 6615 Size: 6739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3528 5853 6037 1305 Size: 2223 2325 184 2007 Fragments arranged by size: 2325 2223 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4917 Fragments arranged by size: 4917 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3701 4697 5056 5215 518 Size: 648 2535 996 359 159 2042 Fragments arranged by size: 2535 2042 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3828 4155 4832 5980 6225 1606 Size: 2222 327 677 1148 245 2120 Fragments arranged by size: 2222 2120 1148 677 327 245 NcoI C'CATG_G Cuts at: 513 1065 2258 513 Size: 552 1193 4994 Fragments arranged by size: 4994 1193 552 NdeI CA'TA_TG Cuts at: 387 2544 387 Size: 2157 4582 NheI G'CTAG_C Cuts at: 1052 3953 1052 Size: 2901 3838 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NsiI A_TGCA'T Cuts at: 3501 3501 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 3322 4097 5642 1132 Size: 97 2093 775 1545 2229 Fragments arranged by size: 2229 2093 1545 775 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6739 PciI A'CATG_T Cuts at: 5638 5638 Size: 6739 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6739 PfoI T'CCnGG_A Cuts at: 2404 2404 Size: 6739 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6321 6353 4808 Size: 32 1481 32 5194 Fragments arranged by size: 5194 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2399 2399 Size: 6739 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6739 PsiI TTA'TAA Cuts at: 2759 4309 2759 Size: 1550 5189 PstI C_TGCA'G Cuts at: 830 3865 4091 830 Size: 3035 226 3478 Fragments arranged by size: 3478 3035 226 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 4987 6699 4987 Size: 1712 5027 SexAI A'CCwGG_T Cuts at: 2333 3908 2333 Size: 1575 5164 SfcI C'TryA_G Cuts at: 826 1045 2901 3477 3861 4087 5222 5903 6094 Size: 219 1856 576 384 226 1135 681 191 Cuts at: 6094 826 Size: 1471 Fragments arranged by size: 1856 1471 1135 681 576 384 226 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2122 2346 3888 4843 5744 6006 Size: 197 926 179 224 1542 955 901 262 Cuts at: 6006 820 Size: 1553 Fragments arranged by size: 1553 1542 955 926 901 262 224 197 179 SnaBI TAC'GTA Cuts at: 493 4036 4530 493 Size: 3543 494 2702 Fragments arranged by size: 3543 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6739 SphI G_CATG'C Cuts at: 1132 4097 1132 Size: 2965 3774 SspI AAT'ATT Cuts at: 5 52 2842 4663 5 Size: 47 2790 1821 2081 Fragments arranged by size: 2790 2081 1821 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6739 StyI C'CwwG_G Cuts at: 513 1065 2258 3047 3532 3979 4178 513 Size: 552 1193 789 485 447 199 3074 Fragments arranged by size: 3074 1193 789 552 485 447 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2251 3866 4766 4783 4936 5121 5566 Size: 1255 671 1615 900 17 153 185 445 Cuts at: 5566 325 Size: 1498 Fragments arranged by size: 1615 1498 1255 900 671 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 4985 6697 Size: 274 80 33 51 1443 484 2524 1712 Cuts at: 6697 96 Size: 138 Fragments arranged by size: 2524 1712 1443 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6699 6731 6699 Size: 32 6707 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6739 XbaI T'CTAG_A Cuts at: 2068 2953 4075 2068 Size: 885 1122 4732 Fragments arranged by size: 4732 1122 885 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3149 3149 Size: 6739 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6739 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 4868 4868 Size: 6739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4547 276 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI