Restriction map for
pFN21ASDB0062
(Linear) (Six-base) MAPSORT of: KIBB0062.seq Check: 5712 from: 1 to: 2010
KIBB0062.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1753 2010
Size: 1140 613 257
Fragments arranged by size:
1140 613 257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 290 2010
Size: 290 1720
AlwNI CAG_nnn'CTG
Cuts at: 0 1057 2010
Size: 1057 953
ApoI r'AATT_y
Cuts at: 0 204 791 856 1172 1381 2010
Size: 204 587 65 316 209 629
Fragments arranged by size:
629 587 316 209 204 65
AseI AT'TA_AT
Cuts at: 0 367 452 2010
Size: 367 85 1558
Fragments arranged by size:
1558 367 85
AvaI C'yCGr_G
Cuts at: 0 1490 2010
Size: 1490 520
BanI G'GyrC_C
Cuts at: 0 837 2010
Size: 837 1173
BbsI GAAGACnn'nnnn_
Cuts at: 0 1994 2010
Size: 1994 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 1245 2010
Size: 1245 765
BfrBI ATG'CAT
Cuts at: 0 1476 2010
Size: 1476 534
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 2010
Size: 27 1983
BmtI G_CTAG'C
Cuts at: 0 1934 2010
Size: 1934 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1322 2010
Size: 970 352 688
Fragments arranged by size:
970 688 352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 308 1850 2010
Size: 84 224 1542 160
Fragments arranged by size:
1542 224 160 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 1895 2010
Size: 1895 115
BsaBI GATnn'nnATC
Cuts at: 0 764 2010
Size: 764 1246
BsaHI Gr'CG_yC
Cuts at: 0 59 2010
Size: 59 1951
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 363 393 2010
Size: 363 30 1617
Fragments arranged by size:
1617 363 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1288 1529 2010
Size: 1288 241 481
Fragments arranged by size:
1288 481 241
BseYI C'CCAG_C
Cuts at: 0 1817 1940 2010
Size: 1817 123 70
Fragments arranged by size:
1817 123 70
BsmI GAATG_Cn'
Cuts at: 0 1476 2010
Size: 1476 534
BsmBI CGTCTCn'nnnn_
Cuts at: 0 66 2010
Size: 66 1944
BsrDI GCAATG_nn'
Cuts at: 0 1503 2010
Size: 1503 507
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1126 1717 1825 2010
Size: 1126 591 108 185
Fragments arranged by size:
1126 591 185 108
BstYI r'GATC_y
Cuts at: 0 355 2010
Size: 355 1655
Bsu36I CC'TnA_GG
Cuts at: 0 1271 2010
Size: 1271 739
BtgI C'CryG_G
Cuts at: 0 235 2010
Size: 235 1775
DraI TTT'AAA
Cuts at: 0 1046 2010
Size: 1046 964
EaeI y'GGCC_r
Cuts at: 0 1021 2010
Size: 1021 989
EarI CTCTTCn'nnn_
Cuts at: 0 794 1159 1898 2010
Size: 794 365 739 112
Fragments arranged by size:
794 739 365 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1140 1322 1753 2010
Size: 970 170 182 431 257
Fragments arranged by size:
970 431 257 182 170
EcoO109I rG'GnC_Cy
Cuts at: 0 376 2010
Size: 376 1634
EcoRV GAT'ATC
Cuts at: 0 963 1401 2010
Size: 963 438 609
Fragments arranged by size:
963 609 438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1211 1758 1837 2010
Size: 1211 547 79 173
Fragments arranged by size:
1211 547 173 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 699 731 2010
Size: 699 32 1279
Fragments arranged by size:
1279 699 32
HincII GTy'rAC
Cuts at: 0 24 2010
Size: 24 1986
HindIII A'AGCT_T
Cuts at: 0 513 2010
Size: 513 1497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1505 2010
Size: 1505 505
MslI CAynn'nnrTG
Cuts at: 0 1678 2010
Size: 1678 332
MspA1I CmG'CkG
Cuts at: 0 1805 2010
Size: 1805 205
NcoI C'CATG_G
Cuts at: 0 235 2010
Size: 235 1775
NdeI CA'TA_TG
Cuts at: 0 521 2010
Size: 521 1489
NheI G'CTAG_C
Cuts at: 0 1930 2010
Size: 1930 80
NsiI A_TGCA'T
Cuts at: 0 1478 2010
Size: 1478 532
NspI r_CATG'y
Cuts at: 0 1299 2010
Size: 1299 711
PfoI T'CCnGG_A
Cuts at: 0 381 2010
Size: 381 1629
PpuMI rG'GwC_Cy
Cuts at: 0 376 2010
Size: 376 1634
PsiI TTA'TAA
Cuts at: 0 736 2010
Size: 736 1274
PstI C_TGCA'G
Cuts at: 0 1842 2010
Size: 1842 168
SexAI A'CCwGG_T
Cuts at: 0 310 1885 2010
Size: 310 1575 125
Fragments arranged by size:
1575 310 125
SfcI C'TryA_G
Cuts at: 0 878 1454 1838 2010
Size: 878 576 384 172
Fragments arranged by size:
878 576 384 172
SmlI C'TyrA_G
Cuts at: 0 99 323 1865 2010
Size: 99 224 1542 145
Fragments arranged by size:
1542 224 145 99
SspI AAT'ATT
Cuts at: 0 819 2010
Size: 819 1191
StyI C'CwwG_G
Cuts at: 0 235 1024 1509 1956 2010
Size: 235 789 485 447 54
Fragments arranged by size:
789 485 447 235 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 228 1843 2010
Size: 228 1615 167
Fragments arranged by size:
1615 228 167
TatI w'GTAC_w
Cuts at: 0 438 2010
Size: 438 1572
XbaI T'CTAG_A
Cuts at: 0 45 930 2010
Size: 45 885 1080
Fragments arranged by size:
1080 885 45
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1126 2010
Size: 1126 884
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AseI AvaI BanI BbsI
BciVI BfrBI BmrI BmtI BpmI BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsmI BsmBI BsrDI BstXI
BstYI Bsu36I BtgI DraI EaeI EarI Eco57MI EcoO109I
EcoRV FalI Hin4I HincII HindIII MmeI MslI MspA1I
NcoI NdeI NheI NsiI NspI PfoI PpuMI PsiI
PstI SexAI SfcI SmlI SspI StyI TaqII TatI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BplI Bpu10I BsaAI
BsaWI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NgoMIV NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XhoI XmaI
XmnI ZraI