Restriction map for
pFN21ASDB0059
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BsaAI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BstBI ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XbaI
XhoI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AccI 3070 GTATAC GT^[AC][GT]AC
AcsI 1984 GAATTC [GA]^AATT[CT]
Alw44I 419 GTGCAC G^TGCAC
ApaLI 419 GTGCAC G^TGCAC
ApoI 1984 GAATTC [GA]^AATT[CT]
Asp700I 1227 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 3154 CTAG C^TAG
BglII 1922 AGATCT A^GATCT
BsaBI 3385 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1598 ACCGGA [AT]^CCGG[AT]
BsmI 2223 GAATGC GAATGC(1/-1)
BspHI 2598 TCATGA T^CATGA
BssSI 2390 CACGAG CACGAG(-5/-1)
Bst1107I 3070 GTATAC GTA^TAC
BstZ17I 3070 GTATAC GTA^TAC
DrdI 1510 GACCTAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 888 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1984 GAATTC G^AATTC
HindIII 1753 AAGCTT A^AGCTT
MaeI 3154 CTAG C^TAG
MamI 3385 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 3153 GCTAGC G^CTAGC
PstI 1939 CTGCAG CTGCA^G
RcaI 2598 TCATGA T^CATGA
ScaI 2960 AGTACT AGT^ACT
XmnI 1227 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AcyI 557 GACGCC G[GA]^CG[CT]C
1717 GACGCC
AocI 1512 CCTAAGG CC^T[ACGT]AGG
3297 CCTGAGG
AspI 1702 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1773 GACCTTGTC
AviII 125 TGCGCA TGC^GCA
890 TGCGCA
BcgI 146 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
249 GCAGGCGAATCG
BlpI 495 GCTCAGC GC^T[ACGT]AGC
834 GCTGAGC
Bpu1102I 495 GCTCAGC GC^T[ACGT]AGC
834 GCTGAGC
BsaHI 557 GACGCC G[GA]^CG[CT]C
1717 GACGCC
BsaI 2320 GAGACC GGTCTC(1/5)
1440 GGTCTC
BsrBI 2497 GAGCGG CCGCTC(-3/-3)
2776 GAGCGG
BsrDI 1533 CATTGC GCAATG(2/0)
2424 CATTGC
BsrFI 176 ACCGGC [GA]^CCGG[CT]
743 ACCGGC
BstAPI 1198 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2367 GCAGGAGCTGC
BstEII 2070 GGTCACC G^GT[ACGT]ACC
2277 GGTCACC
BstPI 2070 GGTCACC G^GT[ACGT]ACC
2277 GGTCACC
Bsu36I 1512 CCTAAGG CC^T[ACGT]AGG
3297 CCTGAGG
CelII 495 GCTCAGC GC^T[ACGT]AGC
834 GCTGAGC
Cfr10I 176 ACCGGC [GA]^CCGG[CT]
743 ACCGGC
DraIII 1677 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2033 CACTGAGTG
Eco81I 1512 CCTAAGG CC^T[ACGT]AGG
3297 CCTGAGG
EcoO65I 2070 GGTCACC G^GT[ACGT]ACC
2277 GGTCACC
FspI 125 TGCGCA TGC^GCA
890 TGCGCA
Hin1I 557 GACGCC G[GA]^CG[CT]C
1717 GACGCC
MaeII 110 ACGT A^CGT
1630 ACGT
NspI 2792 GCATGT [GA]CATG^[CT]
2919 GCATGT
PflFI 1702 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1773 GACCTTGTC
PpuMI 503 AGGACCT [GA]G^G[AT]CC[CT]
1166 GGGACCT
SmaI 1967 CCCGGG CCC^GGG
2852 CCCGGG
StuI 267 AGGCCT AGG^CCT
1667 AGGCCT
Tth111I 1702 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1773 GACCTTGTC
XmaCI 1967 CCCGGG C^CCGGG
2852 CCCGGG
XmaI 1967 CCCGGG C^CCGGG
2852 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII